Restriction map for pF1KB3338
 (Circular) (Six-base) MAPSORT of: pF1KB3338.seq  Check: 2286  from: 1  to: 6596



pF1KB3338  6596 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2518    2518 
   Size:       6596
 
AatII G_ACGT'C

Cuts at:   3194    3194 
   Size:       6596
 
AccI GT'mk_AC

Cuts at:   1763    2870    3398    3634    1763 
   Size:       1107     528     236    4725

  Fragments arranged by size: 

                4725    1107     528     236
 
Acc65I G'GTAC_C

Cuts at:   3612    3612 
   Size:       6596
 
AclI AA'CG_TT

Cuts at:   6436    6436 
   Size:       6596
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    715    1155    2263    3186    3253    3548    4421    4853    5600 
   Size:        440    1108     923      67     295     873     432     747

Cuts at:   5600    5868     715 
   Size:        268    1443

  Fragments arranged by size: 

                1443    1108     923     873     747     440     432     295
                 268      67
 
AflIII A'CryG_T

Cuts at:    368    1040    1280    3948    5052    6029     368 
   Size:        672     240    2668    1104     977     935

  Fragments arranged by size: 

                2668    1104     977     935     672     240
 
AgeI A'CCGG_T

Cuts at:   2015    4984    2015 
   Size:       2969    3627
 
AleI CACnn'nnGTG

Cuts at:   1417    3322    1417 
   Size:       1905    4691
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1027    1059    1027 
   Size:         32    6564
 
AlwNI CAG_nnn'CTG

Cuts at:    446     747    1005    1362    1866    2343    2475    2684    3901 
   Size:        301     258     357     504     477     132     209    1217

Cuts at:   3901    5468     446 
   Size:       1567    1574

  Fragments arranged by size: 

                1574    1567    1217     504     477     357     301     258
                 209     132
 
ApaI G_GGCC'C

Cuts at:    843    1377    1545    1734    1923     843 
   Size:        534     168     189     189    5516

  Fragments arranged by size: 

                5516     534     189     189     168
 
ApaLI G'TGCA_C

Cuts at:   5366    5366 
   Size:       6596
 
ApoI r'AATT_y

Cuts at:   3600    5949    6205    3600 
   Size:       2349     256    3991

  Fragments arranged by size: 

                3991    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6596
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6596
 
AvaI C'yCGr_G

Cuts at:    732    1565    1734    1922    3616     732 
   Size:        833     169     188    1694    3712

  Fragments arranged by size: 

                3712    1694     833     188     169
 
AvrII C'CTAG_G

Cuts at:   1597    1597 
   Size:       6596
 
BamHI G'GATC_C

Cuts at:    753    3621     753 
   Size:       2868    3728
 
BanI G'GyrC_C

Cuts at:   1237    2725    3612    4026    4274    4309    1237 
   Size:       1488     887     414     248      35    3524

  Fragments arranged by size: 

                3524    1488     887     414     248      35
 
BanII G_rGCy'C

Cuts at:    493     843    1267    1377    1545    1734    1923    2284    2324 
   Size:        350     424     110     168     189     189     361      40

Cuts at:   2324    2368    2491    2501    2604    2719    2852    3366    3441 
   Size:         44     123      10     103     115     133     514      75

Cuts at:   3441    3610    4640     493 
   Size:        169    1030    2449

  Fragments arranged by size: 

                2449    1030     514     424     361     350     189     189
                 169     168     133     123     115     110     103      75
                  44      40      10
 
BbeI G_GCGC'C

Cuts at:   4030    4278    4030 
   Size:        248    6348
 
BbsI GAAGACnn'nnnn_

Cuts at:    555    3395    3536     555 
   Size:       2840     141    3615

  Fragments arranged by size: 

                3615    2840     141
 
BbvCI CC'TCA_GC

Cuts at:   2452    2977    2452 
   Size:        525    6071
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2561    2595    2561 
   Size:         34    6562
 
BciVI GTATCCnnnnn_n'

Cuts at:   1259    4494    5255    6596    1259 
   Size:       3235     761    1341    1259

  Fragments arranged by size: 

                3235    1341    1259     761
 
BclI T'GATC_A

Cuts at:   2879    4116    2879 
   Size:       1237    5359
 
BglI GCCn_nnn'nGGC

Cuts at:   1729    1918    1729 
   Size:        189    6407
 
BglII A'GATC_T

Cuts at:   1100    2988    4111    6148    1100 
   Size:       1888    1123    2037    1548

  Fragments arranged by size: 

                2037    1888    1548    1123
 
BlpI GC'TnA_GC

Cuts at:   1020    2951    3708    1020 
   Size:       1931     757    3908

  Fragments arranged by size: 

                3908    1931     757
 
Bme1580I G_kGCm'C

Cuts at:    655     843    1377    1545    1734    1923    2043    2628    2654 
   Size:        188     534     168     189     189     120     585      26

Cuts at:   2654    4221    4314    5370     655 
   Size:       1567      93    1056    1881

  Fragments arranged by size: 

                1881    1567    1056     585     534     189     189     188
                 168     120      93      26
 
BmgBI CAC'GTC

Cuts at:   1719    1719 
   Size:       6596
 
BmrI ACTGGGnnnn_n'

Cuts at:   1116    2030    2230    3808    3889    4224    6368    1116 
   Size:        914     200    1578      81     335    2144    1344

  Fragments arranged by size: 

                2144    1578    1344     914     335     200      81
 
BmtI G_CTAG'C

Cuts at:   1339    1339 
   Size:       6596
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3119    4963    3119 
   Size:       1844    4752
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    286    1253    2515    2866    3535     286 
   Size:        967    1262     351     669    3347

  Fragments arranged by size: 

                3347    1262     967     669     351
 
Bpu10I CC'TnA_GC

Cuts at:   1942    2452    2977    1942 
   Size:        510     525    5561

  Fragments arranged by size: 

                5561     525     510
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3772    4651    5143    5441    3772 
   Size:        879     492     298    4927

  Fragments arranged by size: 

                4927     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   6300    6300 
   Size:       6596
 
BsaAI yAC'GTr

Cuts at:   1041    3949    1041 
   Size:       2908    3688
 
BsaBI GATnn'nnATC

Cuts at:      6     556    4110    6147       6 
   Size:        550    3554    2037     455

  Fragments arranged by size: 

                3554    2037     550     455
 
BsaHI Gr'CG_yC

Cuts at:    524    2057    3191    4027    4275    4977    6473     524 
   Size:       1533    1134     836     248     702    1496     647

  Fragments arranged by size: 

                1533    1496    1134     836     702     647     248
 
BsaWI w'CCGG_w

Cuts at:    722    2015    3785    4306    4984    5258    5405    5816     722 
   Size:       1293    1770     521     678     274     147     411    1502

  Fragments arranged by size: 

                1770    1502    1293     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   3181    3211    3181 
   Size:         30    6566
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    190    3229     190 
   Size:       3039    3557
 
BseYI C'CCAG_C

Cuts at:    486     497    1829    2021    2050    3107    3254    3332    4021 
   Size:         11    1332     192      29    1057     147      78     689

Cuts at:   4021    4779    5356    5852     486 
   Size:        758     577     496    1230

  Fragments arranged by size: 

                1332    1230    1057     758     689     577     496     192
                 147      78      29      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     328     421    1630    2278     247 
   Size:         81      93    1209     648    4565

  Fragments arranged by size: 

                4565    1209     648      93      81
 
BsiEI CG_ry'CG

Cuts at:    104    4138    4184    5392     104 
   Size:       4034      46    1208    1308

  Fragments arranged by size: 

                4034    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    261    1937    2491    2501    2852    3366    3610    4388    4578 
   Size:       1676     554      10     351     514     244     778     190

Cuts at:   4578    5370     261 
   Size:        792    1487

  Fragments arranged by size: 

                1676    1487     792     778     554     514     351     244
                 190      10
 
BsmI GAATG_Cn'

Cuts at:   3111    3111 
   Size:       6596
 
Bsp1286I G_dGCh'C

Cuts at:    261     493     655     843    1267    1377    1545    1734    1923 
   Size:        232     162     188     424     110     168     189     189

Cuts at:   1923    1937    2043    2284    2324    2368    2491    2501    2604 
   Size:         14     106     241      40      44     123      10     103

Cuts at:   2604    2628    2654    2719    2852    3366    3441    3610    4221 
   Size:         24      26      65     133     514      75     169     611

Cuts at:   4221    4314    4388    4578    4640    5370     261 
   Size:         93      74     190      62     730    1487

  Fragments arranged by size: 

                1487     730     611     514     424     241     232     190
                 189     189     188     169     168     162     133     123
                 110     106     103      93      75      74      65      62
                  44      40      26      24      14      10
 
BspEI T'CCGG_A

Cuts at:    722    5816     722 
   Size:       5094    1502
 
BspHI T'CATG_A

Cuts at:   5772    5772 
   Size:       6596
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2351    2518    3646    3860    4162    4543    2351 
   Size:        167    1128     214     302     381    4404

  Fragments arranged by size: 

                4404    1128     381     302     214     167
 
BsrBI CCG'CTC

Cuts at:     50    4888    4942    6426    6591      50 
   Size:       4838      54    1484     165      55

  Fragments arranged by size: 

                4838    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1435    3161    3268    4508    1435 
   Size:       1726     107    1240    3523

  Fragments arranged by size: 

                3523    1726    1240     107
 
BsrFI r'CCGG_y

Cuts at:    319    1953    2015    4594    4775    4984     319 
   Size:       1634      62    2579     181     209    1931

  Fragments arranged by size: 

                2579    1931    1634     209     181      62
 
BsrGI T'GTAC_A

Cuts at:   3473    3473 
   Size:       6596
 
BssSI C'ACGA_G

Cuts at:    247    1150    2162    4867    5225     247 
   Size:        903    1012    2705     358    1618

  Fragments arranged by size: 

                2705    1618    1012     903     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2684    3973    2684 
   Size:       1289    5307
 
BstBI TT'CG_AA

Cuts at:   4957    4957 
   Size:       6596
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2043    3420    2043 
   Size:       1377    5219
 
BstYI r'GATC_y

Cuts at:      1     238     698     753     860    1100    2988    3621    4088 
   Size:        237     460      55     107     240    1888     633     467

Cuts at:   4088    4111    4446    4692    5704    5790    5802    6148       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1888    1012     633     467     460     449     346     335
                 246     240     237     107      86      55      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:    356     356 
   Size:       6596
 
BtgI C'CryG_G

Cuts at:    791    1496    1635    1913    2360    2480    2549     791 
   Size:        705     139     278     447     120      69    4838

  Fragments arranged by size: 

                4838     705     447     278     139     120      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1526    4546    4727    4890    1526 
   Size:       3020     181     163    3232

  Fragments arranged by size: 

                3232    3020     181     163
 
BtsI GCAGTG_nn'

Cuts at:    989    1618    1624    2381    2694    2808    3533    3839     989 
   Size:        629       6     757     313     114     725     306    3746

  Fragments arranged by size: 

                3746     757     725     629     313     306     114       6
            
 
ClaI AT'CG_AT

Cuts at:   2958    2958 
   Size:       6596
 
DraI TTT'AAA

Cuts at:   3595    3595 
   Size:       6596
 
DrdI GACnn_nn'nnGTC

Cuts at:   2062    2443    4302    5160    2062 
   Size:        381    1859     858    3498

  Fragments arranged by size: 

                3498    1859     858     381
 
EaeI y'GGCC_r

Cuts at:    278     788    1721    1910    1973    2000    2357    2993    4181 
   Size:        510     933     189      63      27     357     636    1188

Cuts at:   4181    4355    4746    4773    6118     278 
   Size:        174     391      27    1345     756

  Fragments arranged by size: 

                1345    1188     933     756     636     510     391     357
                 189     174      63      27      27
 
EagI C'GGCC_G

Cuts at:   4181    4181 
   Size:       6596
 
EarI CTCTTCn'nnn_

Cuts at:   1204    2021    2383    4619    4829    1204 
   Size:        817     362    2236     210    2971

  Fragments arranged by size: 

                2971    2236     817     362     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    340    4804    5114    5260    6406     340 
   Size:       4464     310     146    1146     530

  Fragments arranged by size: 

                4464    1146     530     310     146
 
EcoICRI GAG'CTC

Cuts at:   2489    2499    2850    3364    3608    2489 
   Size:         10     351     514     244    5477

  Fragments arranged by size: 

                5477     514     351     244      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    286     715    1155    1253    2263    2515    2866    3186    3253 
   Size:        429     440      98    1010     252     351     320      67

Cuts at:   3253    3535    3548    4421    4853    5600    5868     286 
   Size:        282      13     873     432     747     268    1014

  Fragments arranged by size: 

                1014    1010     873     747     440     432     429     351
                 320     282     268     252      98      67      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1345    1596    1888    1345 
   Size:        251     292    6053

  Fragments arranged by size: 

                6053     292     251
 
EcoO109I rG'GnC_Cy

Cuts at:    839     985    1373    1374    1481    1541    1542    1730    1731 
   Size:        146     388       1     107      60       1     188       1

Cuts at:   1731    1881    1919    2436    2667    3063    3735     839 
   Size:        150      38     517     231     396     672    3700

  Fragments arranged by size: 

                3700     672     517     396     388     231     188     150
                 146     107      60      38       1       1       1
 
EcoRI G'AATT_C

Cuts at:   3600    3600 
   Size:       6596
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    720    2853    3501    4615     720 
   Size:       2133     648    1114    2701

  Fragments arranged by size: 

                2701    2133    1114     648
 
FspI TGC'GCA

Cuts at:    309    4377     309 
   Size:       4068    2528
 
HaeII r_GCGC'y

Cuts at:   1027    1867    3457    4030    4278    5300    6279    1027 
   Size:        840    1590     573     248    1022     979    1344

  Fragments arranged by size: 

                1590    1344    1022     979     840     573     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    230     262    1860    1892    2309    2341    3052    3084    3181 
   Size:         32    1598      32     417      32     711      32      97

Cuts at:   3181    3213    4438    4468    4470    4500     230 
   Size:         32    1225      30       2      30    2326

  Fragments arranged by size: 

                2326    1598    1225     711     417      97      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   2694    3635    2694 
   Size:        941    5655
 
HindIII A'AGCT_T

Cuts at:   3478    3478 
   Size:       6596
 
KasI G'GCGC_C

Cuts at:   4026    4274    4026 
   Size:        248    6348
 
KpnI G_GTAC'C

Cuts at:   3616    3616 
   Size:       6596
 
MluI A'CGCG_T

Cuts at:   6029    6029 
   Size:       6596
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    789    5267    5451     789 
   Size:       4478     184    1934

  Fragments arranged by size: 

                4478    1934     184
 
MscI TGG'CCA

Cuts at:   1723    1975    2002    2995    4357    1723 
   Size:        252      27     993    1362    3962

  Fragments arranged by size: 

                3962    1362     993     252      27
 
MslI CAynn'nnrTG

Cuts at:    322     833    1279    1417    1553    2621    3322    3418    4585 
   Size:        511     446     138     136    1068     701      96    1167

Cuts at:   4585    4712     322 
   Size:        127    2206

  Fragments arranged by size: 

                2206    1167    1068     701     511     446     138     136
                 127      96
 
MspA1I CmG'CkG

Cuts at:    313     342    1089    1815    2684    2844    3185    3563    3707 
   Size:         29     747     726     869     160     341     378     144

Cuts at:   3707    4021    4381    5394    5639     313 
   Size:        314     360    1013     245    1270

  Fragments arranged by size: 

                1270    1013     869     747     726     378     360     341
                 314     245     160     144      29
 
NaeI GCC'GGC

Cuts at:   4777    4777 
   Size:       6596
 
NarI GG'CG_CC

Cuts at:   4027    4275    4027 
   Size:        248    6348
 
NgoMIV G'CCGG_C

Cuts at:   4775    4775 
   Size:       6596
 
NheI G'CTAG_C

Cuts at:   1335    1335 
   Size:       6596
 
NspI r_CATG'y

Cuts at:    327     372    1284    2642    3649    4149    5056     327 
   Size:         45     912    1358    1007     500     907    1867

  Fragments arranged by size: 

                1867    1358    1007     912     907     500      45
 
PasI CC'CwG_GG

Cuts at:   1655    2214    2766    1655 
   Size:        559     552    5485

  Fragments arranged by size: 

                5485     559     552
 
PciI A'CATG_T

Cuts at:    368    1280    5052     368 
   Size:        912    3772    1912

  Fragments arranged by size: 

                3772    1912     912
 
PflMI CCAn_nnn'nTGG

Cuts at:   1430    1430 
   Size:       6596
 
PfoI T'CCnGG_A

Cuts at:    191    3209    3339     191 
   Size:       3018     130    3448

  Fragments arranged by size: 

                3448    3018     130
 
PmeI GTTT'AAAC

Cuts at:   3595    3595 
   Size:       6596
 
PmlI CAC'GTG

Cuts at:   1041    1041 
   Size:       6596
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    448     480    5735    5767     448 
   Size:         32    5255      32    1277

  Fragments arranged by size: 

                5255    1277      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    985    1881    2436     985 
   Size:        896     555    5145

  Fragments arranged by size: 

                5145     896     555
 
PshAI GACnn'nnGTC

Cuts at:   2147    2592    2147 
   Size:        445    6151
 
PspOMI G'GGCC_C

Cuts at:    839    1373    1541    1730    1919     839 
   Size:        534     168     189     189    5516

  Fragments arranged by size: 

                5516     534     189     189     168
 
PstI C_TGCA'G

Cuts at:   1094    1850    2471    2689    3179    3643    4328    1094 
   Size:        756     621     218     490     464     685    3362

  Fragments arranged by size: 

                3362     756     685     621     490     464     218
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6596
 
PvuII CAG'CTG

Cuts at:    313    1089    2844    3563    4021    4381     313 
   Size:        776    1755     719     458     360    2528

  Fragments arranged by size: 

                2528    1755     776     719     458     360
 
RsrII CG'GwC_CG

Cuts at:   4791    4791 
   Size:       6596
 
SacI G_AGCT'C

Cuts at:   2491    2501    2852    3366    3610    2491 
   Size:         10     351     514     244    5477

  Fragments arranged by size: 

                5477     514     351     244      10
 
SalI G'TCGA_C

Cuts at:   3633    3633 
   Size:       6596
 
SanDI GG'GwC_CC

Cuts at:    985     985 
   Size:       6596
 
SapI GCTCTTCn'nnn_

Cuts at:   4619    4829    4619 
   Size:        210    6386
 
SbfI CC_TGCA'GG

Cuts at:   3643    3643 
   Size:       6596
 
ScaI AGT'ACT

Cuts at:    682    2908    6113     682 
   Size:       2226    3205    1165

  Fragments arranged by size: 

                3205    2226    1165
 
SexAI A'CCwGG_T

Cuts at:   1361    1361 
   Size:       6596
 
SfcI C'TryA_G

Cuts at:     33    1090    1846    2467    2685    3175    3639    4324    5317 
   Size:       1057     756     621     218     490     464     685     993

Cuts at:   5317    5508      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121    1057     993     756     685     621     490     464
                 218     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1729    1918    1729 
   Size:        189    6407
 
SfoI GGC'GCC

Cuts at:   4028    4276    4028 
   Size:        248    6348
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6596
 
SmaI CCC'GGG

Cuts at:   1736    1924    3618    1736 
   Size:        188    1694    4714

  Fragments arranged by size: 

                4714    1694     188
 
SmlI C'TyrA_G

Cuts at:   3751    4666    5158    5420    3751 
   Size:        915     492     262    4927

  Fragments arranged by size: 

                4927     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6596
 
SphI G_CATG'C

Cuts at:   3649    4149    3649 
   Size:        500    6096
 
StuI AGG'CCT

Cuts at:    776    2465     776 
   Size:       1689    4907
 
StyI C'CwwG_G

Cuts at:    374    1068    1597    2003    3730    4084     374 
   Size:        694     529     406    1727     354    2886

  Fragments arranged by size: 

                2886    1727     694     529     406     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    730    1429    1903    2431    4179    4312    4771    4980     730 
   Size:        699     474     528    1748     133     459     209    2346

  Fragments arranged by size: 

                2346    1748     699     528     474     459     209     133
            
 
TatI w'GTAC_w

Cuts at:    680     890    2906    3326    3473    6111     680 
   Size:        210    2016     420     147    2638    1165

  Fragments arranged by size: 

                2638    2016    1165     420     210     147
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1141    1173    2217    2249    2514    2546    6113    6145    1141 
   Size:         32    1044      32     265      32    3567      32    1592

  Fragments arranged by size: 

                3567    1592    1044     265      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:    559    3394    4393     559 
   Size:       2835     999    2762

  Fragments arranged by size: 

                2835    2762     999
 
XbaI T'CTAG_A

Cuts at:   3627    3627 
   Size:       6596
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2841    4091    2841 
   Size:       1250    5346
 
XmaI C'CCGG_G

Cuts at:   1734    1922    3616    1734 
   Size:        188    1694    4714

  Fragments arranged by size: 

                4714    1694     188
 
ZraI GAC'GTC

Cuts at:   3192    3192 
   Size:       6596
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AlfI     AscI     BaeI    BfrBI    BsiWI
    BsmBI   BssHII   BstEII  BstZ17I    CspCI   DraIII    EcoRV     FseI
    FspAI     HpaI     MfeI     NcoI     NdeI     NotI     NruI     NsiI
     PacI     PsiI    PspXI     PsrI    SacII    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI
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