Restriction map for pF1KB3312
 (Linear) (Six-base) MAPSORT of: KIBB3312.seq  Check: 7559  from: 1  to: 2472



KIBB3312.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1862    2472 
   Size:       1862     610
 
AfeI AGC'GCT

Cuts at:      0    1982    2472 
   Size:       1982     490
 
AflIII A'CryG_T

Cuts at:      0     366    2472 
   Size:        366    2106
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     235     553    2472 
   Size:        235     318    1919

  Fragments arranged by size: 

                1919     318     235
 
AlwNI CAG_nnn'CTG

Cuts at:      0     490    2472 
   Size:        490    1982
 
ApoI r'AATT_y

Cuts at:      0     505     645    1692    1762    1948    2116    2274    2467 
   Size:        505     140    1047      70     186     168     158     193

Cuts at:   2467    2472 
   Size:          5

  Fragments arranged by size: 

                1047     505     193     186     168     158     140      70
                   5
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     545     578    2472 
   Size:        545      33    1894

  Fragments arranged by size: 

                1894     545      33
 
BamHI G'GATC_C

Cuts at:      0     883    2472 
   Size:        883    1589
 
BanII G_rGCy'C

Cuts at:      0     945    1093    2472 
   Size:        945     148    1379

  Fragments arranged by size: 

                1379     945     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     987    2472 
   Size:        987    1485
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2061    2472 
   Size:       2061     411
 
BfrBI ATG'CAT

Cuts at:      0    1909    1968    2472 
   Size:       1909      59     504

  Fragments arranged by size: 

                1909     504      59
 
BglII A'GATC_T

Cuts at:      0    1610    2472 
   Size:       1610     862
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     748    2472 
   Size:        748    1724
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1632    2472 
   Size:       1632     840
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    2472 
   Size:        896    1576
 
Bpu10I CC'TnA_GC

Cuts at:      0     150    2472 
   Size:        150    2322
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     452    2472 
   Size:        452    2020
 
BsaBI GATnn'nnATC

Cuts at:      0    2297    2472 
   Size:       2297     175
 
BsaWI w'CCGG_w

Cuts at:      0     937    2472 
   Size:        937    1535
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2377    2472 
   Size:       2377      95
 
Bsp1286I G_dGCh'C

Cuts at:      0     945    1093    2472 
   Size:        945     148    1379

  Fragments arranged by size: 

                1379     945     148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     227    2472 
   Size:        227    2245
 
BsrBI CCG'CTC

Cuts at:      0     149    2472 
   Size:        149    2323
 
BsrDI GCAATG_nn'

Cuts at:      0     214    2472 
   Size:        214    2258
 
BstBI TT'CG_AA

Cuts at:      0    1872    2472 
   Size:       1872     600
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1706    2472 
   Size:       1706     766
 
BstYI r'GATC_y

Cuts at:      0     486     883    1610    2298    2472 
   Size:        486     397     727     688     174

  Fragments arranged by size: 

                 727     688     486     397     174
 
Bsu36I CC'TnA_GG

Cuts at:      0    1079    2472 
   Size:       1079    1393
 
BtsI GCAGTG_nn'

Cuts at:      0    1117    2472 
   Size:       1117    1355
 
DraI TTT'AAA

Cuts at:      0     431     504    2472 
   Size:        431      73    1968

  Fragments arranged by size: 

                1968     431      73
 
DraIII CAC_nnn'GTG

Cuts at:      0    1006    2472 
   Size:       1006    1466
 
EaeI y'GGCC_r

Cuts at:      0     972    2472 
   Size:        972    1500
 
EarI CTCTTCn'nnn_

Cuts at:      0    1305    2472 
   Size:       1305    1167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1862    2472 
   Size:        896     966     610

  Fragments arranged by size: 

                 966     896     610
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1520    2472 
   Size:       1520     952
 
EcoRV GAT'ATC

Cuts at:      0    1989    2472 
   Size:       1989     483
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     690    1011    2472 
   Size:        690     321    1461

  Fragments arranged by size: 

                1461     690     321
 
HaeII r_GCGC'y

Cuts at:      0    1984    2472 
   Size:       1984     488
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     916     948    2472 
   Size:        916      32    1524

  Fragments arranged by size: 

                1524     916      32
 
HindIII A'AGCT_T

Cuts at:      0     250    2472 
   Size:        250    2222
 
MfeI C'AATT_G

Cuts at:      0     626    2333    2472 
   Size:        626    1707     139

  Fragments arranged by size: 

                1707     626     139
 
MslI CAynn'nnrTG

Cuts at:      0     207    1904    2472 
   Size:        207    1697     568

  Fragments arranged by size: 

                1697     568     207
 
MspA1I CmG'CkG

Cuts at:      0     712    2455    2472 
   Size:        712    1743      17

  Fragments arranged by size: 

                1743     712      17
 
NsiI A_TGCA'T

Cuts at:      0    1911    1970    2472 
   Size:       1911      59     502

  Fragments arranged by size: 

                1911     502      59
 
NspI r_CATG'y

Cuts at:      0     370    1548    2041    2472 
   Size:        370    1178     493     431

  Fragments arranged by size: 

                1178     493     431     370
 
PciI A'CATG_T

Cuts at:      0     366    2472 
   Size:        366    2106
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     790     844     874    2472 
   Size:        790      54      30    1598

  Fragments arranged by size: 

                1598     790      54      30
 
PfoI T'CCnGG_A

Cuts at:      0     873    2472 
   Size:        873    1599
 
PsiI TTA'TAA

Cuts at:      0    1904    2472 
   Size:       1904     568
 
PstI C_TGCA'G

Cuts at:      0      14    1719    2472 
   Size:         14    1705     753

  Fragments arranged by size: 

                1705     753      14
 
PvuII CAG'CTG

Cuts at:      0     712    2455    2472 
   Size:        712    1743      17

  Fragments arranged by size: 

                1743     712      17
 
ScaI AGT'ACT

Cuts at:      0     670    2472 
   Size:        670    1802
 
SfcI C'TryA_G

Cuts at:      0      10    1715    2472 
   Size:         10    1705     757

  Fragments arranged by size: 

                1705     757      10
 
SpeI A'CTAG_T

Cuts at:      0    1147    2472 
   Size:       1147    1325
 
SspI AAT'ATT

Cuts at:      0    1458    1562    2472 
   Size:       1458     104     910

  Fragments arranged by size: 

                1458     910     104
 
StuI AGG'CCT

Cuts at:      0    1351    1705    2472 
   Size:       1351     354     767

  Fragments arranged by size: 

                1351     767     354
 
StyI C'CwwG_G

Cuts at:      0    1166    2472 
   Size:       1166    1306
 
SwaI ATTT'AAAT

Cuts at:      0     504    2472 
   Size:        504    1968
 
TatI w'GTAC_w

Cuts at:      0     102     196     668    1069    1541    2472 
   Size:        102      94     472     401     472     931

  Fragments arranged by size: 

                 931     472     472     401     102      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1193    1225    2472 
   Size:       1193      32    1247

  Fragments arranged by size: 

                1247    1193      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1489    2472 
   Size:       1489     983
 
XmnI GAAnn'nnTTC

Cuts at:      0    1131    1726    2472 
   Size:       1131     595     746

  Fragments arranged by size: 

                1131     746     595
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AlfI    AlwNI     ApoI     BaeI    BamHI
    BanII     BbsI    BciVI    BfrBI    BglII     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaWI    BseRI Bsp1286I    BspMI    BsrBI
    BsrDI    BstBI    BstXI    BstYI   Bsu36I     BtsI     DraI   DraIII
     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I
  HindIII     MfeI     MslI   MspA1I     NsiI     NspI     PciI    PflMI
     PfoI     PsiI     PstI    PvuII     ScaI     SfcI     SpeI     SspI
     StuI     StyI     SwaI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BanI     BbeI    BbvCI     BcgI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmtI    BpuEI    BsaAI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI    TaqII
  Tth111I     XbaI     XhoI     XmaI     ZraI
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