Restriction map for pF1KB3294
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BfrI        BglI        BglII       BlnI        
BsaBI       BseAI       BsiWI       BsmBI       BspEI       
BspHI       BspLU11I    BsrDI       BstAPI      BstBI       
Bsu36I      CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MroI        MunI        NcoI        NdeI        
NheI        NotI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
Sse8387I    StyI        SwaI        Tth111I     Van91I      
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          736  GTATAC               GT^[AC][GT]AC
BalI         2515  TGGCCA               TGG^CCA
BcgI         1097  GCACCGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2524  GTATCC               GTATCC(6/5)
BlpI          513  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      513  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1519  TACGTG               [CT]AC^GT[GA]
BsaI         2761  GAGACC               GGTCTC(1/5)
BsiEI         270  CGATCG               CG[GA][CT]^CG
BsmI         2477  GCATTC               GAATGC(1/-1)
BspDI         268  ATCGAT               AT^CGAT
Bst1107I      736  GTATAC               GTA^TAC
BstEII       1272  GGTGACC              G^GT[ACGT]ACC
BstPI        1272  GGTGACC              G^GT[ACGT]ACC
BstZ17I       736  GTATAC               GTA^TAC
CelII         513  GCTCAGC              GC^T[ACGT]AGC
ClaI          268  ATCGAT               AT^CGAT
DraI         2605  TTTAAA               TTT^AAA
EarI          497  CTCTTC               CTCTTC(1/4)
EcoO65I      1272  GGTGACC              G^GT[ACGT]ACC
Ksp632I       497  CTCTTC               CTCTTC(1/4)
MluI         1811  ACGCGT               A^CGCGT
MluNI        2515  TGGCCA               TGG^CCA
MscI         2515  TGGCCA               TGG^CCA
NspI         2444  GCATGC               [GA]CATG^[CT]
PaeR7I       1703  CTCGAG               C^TCGAG
PshAI        1899  GACTCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          270  CGATCG               CGAT^CG
PvuII        2595  CAGCTG               CAG^CTG
SapI          496  GCTCTTC              GCTCTTC(1/4)
SgrAI        1685  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI           32  CCCGGG               CCC^GGG
SmlI         1703  CTCGAG               C^T[CT][GA]AG
SphI         2444  GCATGC               GCATG^C
StuI         2322  AGGCCT               AGG^CCT
XbaI          433  TCTAGA               T^CTAGA
XcmI         1316  CCAGGGTGTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1703  CTCGAG               C^TCGAG
XmaCI          32  CCCGGG               C^CCGGG
XmaI           32  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I       1528  GTGCAC               G^TGCAC
             2458  GTGCAC              
AlwNI         148  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1561  CAGCCCCTG           
ApaI         2061  GGGCCC               GGGCC^C
             2286  GGGCCC              
ApaLI        1528  GTGCAC               G^TGCAC
             2458  GTGCAC              
BbeI         1698  GGCGCC               GGCGC^C
             2022  GGCGCC              
BbsI          394  GTCTTC               GAAGAC(2/6)
             2034  GTCTTC              
BpuAI         394  GTCTTC               GAAGAC(2/6)
             2034  GTCTTC              
BsaWI         657  ACCGGA               [AT]^CCGG[AT]
             2635  TCCGGT              
BsgI          303  GTGCAG               GTGCAG(16/14)
             1724  GTGCAG              
BsmAI         757  GAGAC                GTCTC(1/5)
             2761  GAGAC               
Bsp1407I     1877  TGTACA               T^GTACA
             2150  TGTACA              
BsrGI        1877  TGTACA               T^GTACA
             2150  TGTACA              
BstXI        2123  CCAGCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2225  CCAGCGCGTTGG        
EcoRI         258  GAATTC               G^AATTC
              444  GAATTC              
HincII       2086  GTCAAC               GT[CT]^[GA]AC
             2584  GTCAAC              
HindII       2086  GTCAAC               GT[CT]^[GA]AC
             2584  GTCAAC              
KasI         1698  GGCGCC               G^GCGCC
             2022  GGCGCC              
MslI         1113  CATCACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2211  CACTCTGGTG          
MspA1I        337  CCGCTG               C[AC]G^C[GT]G
             2595  CAGCTG              
NarI         1698  GGCGCC               GG^CGCC
             2022  GGCGCC              
NruI          273  TCGCGA               TCG^CGA
             1242  TCGCGA              
PpuMI         149  AGGACCT              [GA]G^G[AT]CC[CT]
              215  GGGACCT             
SfoI         1698  GGCGCC               GGC^GCC
             2022  GGCGCC              
SspBI        1877  TGTACA               T^GTACA
             2150  TGTACA              
SspI          417  AATATT               AAT^ATT
              593  AATATT              
TfiI          430  GAATC                G^A[AT]TC
              886  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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