Restriction map for
pF1KB3287
(Circular) (Six-base) MAPSORT of: pF1KB3287.seq Check: 868 from: 1 to: 5981
pF1KB3287 5981 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3019 3019
Size: 5981
Acc65I G'GTAC_C
Cuts at: 2997 2997
Size: 5981
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1420 2464 3806 4238 4591 5253 814
Size: 606 1044 1342 432 353 662 1542
Fragments arranged by size:
1542 1342 1044 662 606 432 353
AflIII A'CryG_T
Cuts at: 2186 3333 5133 5414 2186
Size: 1147 1800 281 2753
Fragments arranged by size:
2753 1800 1147 281
AhdI GACnn_n'nnGTC
Cuts at: 672 672
Size: 5981
AleI CACnn'nnGTG
Cuts at: 1841 1841
Size: 5981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2074 2074
Size: 5981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1864 1896 1864
Size: 32 5949
AlwNI CAG_nnn'CTG
Cuts at: 1071 1898 2820 2898 3286 4724 1071
Size: 827 922 78 388 1438 2328
Fragments arranged by size:
2328 1438 922 827 388 78
ApaLI G'TGCA_C
Cuts at: 4819 4819
Size: 5981
ApoI r'AATT_y
Cuts at: 1198 2148 2250 2985 5334 5590 1198
Size: 950 102 735 2349 256 1589
Fragments arranged by size:
2349 1589 950 735 256 102
AscI GG'CGCG_CC
Cuts at: 2700 2700
Size: 5981
AseI AT'TA_AT
Cuts at: 20 20
Size: 5981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 698 1534 2734 2875 2971 3001 698
Size: 836 1200 141 96 30 3678
Fragments arranged by size:
3678 1200 836 141 96 30
BamHI G'GATC_C
Cuts at: 563 3006 563
Size: 2443 3538
BanI G'GyrC_C
Cuts at: 123 1138 1546 2997 3411 3659 3694 123
Size: 1015 408 1451 414 248 35 2410
Fragments arranged by size:
2410 1451 1015 414 408 248 35
BanII G_rGCy'C
Cuts at: 1933 2801 2972 2995 4025 1933
Size: 868 171 23 1030 3889
Fragments arranged by size:
3889 1030 868 171 23
BbeI G_GCGC'C
Cuts at: 127 3415 3663 127
Size: 3288 248 2445
Fragments arranged by size:
3288 2445 248
BbvCI CC'TCA_GC
Cuts at: 1300 1300
Size: 5981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2352 2386 2894 2928 90
Size: 34 2228 34 508 34 3143
Fragments arranged by size:
3143 2228 508 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2692 3879 4935 5981 2692
Size: 1187 1056 1046 2692
Fragments arranged by size:
2692 1187 1056 1046
BclI T'GATC_A
Cuts at: 1997 3501 1997
Size: 1504 4477
BglI GCCn_nnn'nGGC
Cuts at: 1774 2026 2721 2842 1774
Size: 252 695 121 4913
Fragments arranged by size:
4913 695 252 121
BglII A'GATC_T
Cuts at: 1124 1590 3496 5533 1124
Size: 466 1906 2037 1572
Fragments arranged by size:
2037 1906 1572 466
BlpI GC'TnA_GC
Cuts at: 2767 3093 2767
Size: 326 5655
Bme1580I G_kGCm'C
Cuts at: 998 1848 2063 2476 2758 2907 3606 3699 4823
Size: 850 215 413 282 149 699 93 1124
Cuts at: 4823 998
Size: 2156
Fragments arranged by size:
2156 1124 850 699 413 282 215 149
93
BmrI ACTGGGnnnn_n'
Cuts at: 1013 3193 3274 3609 5753 1013
Size: 2180 81 335 2144 1241
Fragments arranged by size:
2180 2144 1241 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 4348 252
Size: 4096 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 847 871 2112 2395 2455 2959 399
Size: 448 24 1241 283 60 504 3421
Fragments arranged by size:
3421 1241 504 448 283 60 24
Bpu10I CC'TnA_GC
Cuts at: 1300 1300
Size: 5981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 703 1447 3157 4036 4750 5048 201
Size: 502 744 1710 879 714 298 1134
Fragments arranged by size:
1710 1134 879 744 714 502 298
BsaI GGTCTCn'nnnn_
Cuts at: 5685 5685
Size: 5981
BsaAI yAC'GTr
Cuts at: 1287 3334 1287
Size: 2047 3934
BsaBI GATnn'nnATC
Cuts at: 6 2002 3495 5532 6
Size: 1996 1493 2037 455
Fragments arranged by size:
2037 1996 1493 455
BsaHI Gr'CG_yC
Cuts at: 124 1745 1813 1976 3412 3660 4362 5858 124
Size: 1621 68 163 1436 248 702 1496 247
Fragments arranged by size:
1621 1496 1436 702 248 247 163 68
BsaWI w'CCGG_w
Cuts at: 1076 1905 3170 3691 4369 4780 4927 5201 1076
Size: 829 1265 521 678 411 147 274 1856
Fragments arranged by size:
1856 1265 829 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1864 1894 2110 2140 1864
Size: 30 216 30 5705
Fragments arranged by size:
5705 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 706 1008 2242 2752 2944 493
Size: 213 302 1234 510 192 3530
Fragments arranged by size:
3530 1234 510 302 213 192
BseYI C'CCAG_C
Cuts at: 282 408 529 559 2203 3406 4164 4829 5237
Size: 126 121 30 1644 1203 758 665 408
Cuts at: 5237 282
Size: 1026
Fragments arranged by size:
1644 1203 1026 758 665 408 126 121
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 567 1084 2065 2838 237
Size: 330 517 981 773 3380
Fragments arranged by size:
3380 981 773 517 330
BsiEI CG_ry'CG
Cuts at: 104 1910 2749 2911 3523 3569 4799 104
Size: 1806 839 162 612 46 1230 1286
Fragments arranged by size:
1806 1286 1230 839 612 162 46
BsiHKAI G_wGCw'C
Cuts at: 2224 2995 3773 3963 4823 2224
Size: 771 778 190 860 3382
Fragments arranged by size:
3382 860 778 771 190
BsmI GAATG_Cn'
Cuts at: 622 1676 1987 622
Size: 1054 311 4616
Fragments arranged by size:
4616 1054 311
BsmBI CGTCTCn'nnnn_
Cuts at: 123 123
Size: 5981
Bsp1286I G_dGCh'C
Cuts at: 998 1848 1933 2063 2224 2476 2758 2801 2907
Size: 850 85 130 161 252 282 43 106
Cuts at: 2907 2972 2995 3606 3699 3773 3963 4025 4823
Size: 65 23 611 93 74 190 62 798
Cuts at: 4823 998
Size: 2156
Fragments arranged by size:
2156 850 798 611 282 252 190 161
130 106 93 85 74 65 62 43
23
BspHI T'CATG_A
Cuts at: 1148 1658 4413 1148
Size: 510 2755 2716
Fragments arranged by size:
2755 2716 510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1166 3031 3245 3547 3928 1166
Size: 1865 214 302 381 3219
Fragments arranged by size:
3219 1865 381 302 214
BsrBI CCG'CTC
Cuts at: 50 231 714 4273 4327 5811 5976 50
Size: 181 483 3559 54 1484 165 55
Fragments arranged by size:
3559 1484 483 181 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1434 3893 1434
Size: 2459 3522
BsrFI r'CCGG_y
Cuts at: 1186 1853 2693 3979 4160 1186
Size: 667 840 1286 181 3007
Fragments arranged by size:
3007 1286 840 667 181
BssHII G'CGCG_C
Cuts at: 2700 2700
Size: 5981
BssSI C'ACGA_G
Cuts at: 151 4252 4960 151
Size: 4101 708 1172
Fragments arranged by size:
4101 1172 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3358 3358
Size: 5981
BstBI TT'CG_AA
Cuts at: 4342 4342
Size: 5981
BstEII G'GTnAC_C
Cuts at: 2643 2643
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 246 378 1377 2571 246
Size: 132 999 1194 3656
Fragments arranged by size:
3656 1194 999 132
BstYI r'GATC_y
Cuts at: 1 362 563 725 1124 1478 1499 1590 2438
Size: 361 201 162 399 354 21 91 848
Cuts at: 2438 3006 3473 3496 3831 4077 4383 4395 4481
Size: 568 467 23 335 246 306 12 86
Cuts at: 4481 5533 1
Size: 1052 449
Fragments arranged by size:
1052 848 568 467 449 399 361 354
335 306 246 201 162 91 86 23
21 12
BtgI C'CryG_G
Cuts at: 209 2749 2847 209
Size: 2540 98 3343
Fragments arranged by size:
3343 2540 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1923 3931 4112 4275 1923
Size: 2008 181 163 3629
Fragments arranged by size:
3629 2008 181 163
BtsI GCAGTG_nn'
Cuts at: 3224 3224
Size: 5981
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 420 455 2158 2193 420
Size: 35 1703 35 4208
Fragments arranged by size:
4208 1703 35 35
DraI TTT'AAA
Cuts at: 626 2980 626
Size: 2354 3627
DrdI GACnn_nn'nnGTC
Cuts at: 3687 5031 3687
Size: 1344 4637
EaeI y'GGCC_r
Cuts at: 248 1188 1275 1775 2018 2402 2561 2908 3566
Size: 940 87 500 243 384 159 347 658
Cuts at: 3566 3740 4131 4158 5503 248
Size: 174 391 27 1345 726
Fragments arranged by size:
1345 940 726 658 500 391 384 347
243 174 159 87 27
EagI C'GGCC_G
Cuts at: 2908 3566 2908
Size: 658 5323
EarI CTCTTCn'nnn_
Cuts at: 1351 1759 1798 2212 4004 4214 1351
Size: 408 39 414 1792 210 3118
Fragments arranged by size:
3118 1792 414 408 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1492 4189 4931 5077 5791 1492
Size: 2697 742 146 714 1682
Fragments arranged by size:
2697 1682 742 714 146
EcoICRI GAG'CTC
Cuts at: 2993 2993
Size: 5981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 814 847 871 1420 2112 2395 2455 2464
Size: 415 33 24 549 692 283 60 9
Cuts at: 2464 2959 3806 4238 4591 5253 399
Size: 495 847 432 353 662 1127
Fragments arranged by size:
1127 847 692 662 549 495 432 415
353 283 60 33 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 370 793 2671 370
Size: 423 1878 3680
Fragments arranged by size:
3680 1878 423
EcoO109I rG'GnC_Cy
Cuts at: 453 702 1296 2331 2895 3120 453
Size: 249 594 1035 564 225 3314
Fragments arranged by size:
3314 1035 594 564 249 225
EcoRI G'AATT_C
Cuts at: 1198 2250 2985 1198
Size: 1052 735 4194
Fragments arranged by size:
4194 1052 735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5981
FspI TGC'GCA
Cuts at: 1162 2656 3762 1162
Size: 1494 1106 3381
Fragments arranged by size:
3381 1494 1106
HaeII r_GCGC'y
Cuts at: 127 2745 3415 3663 4893 5664 127
Size: 2618 670 248 1230 771 444
Fragments arranged by size:
2618 1230 771 670 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 3823 3853 3855 3885 658
Size: 32 3133 30 2 30 2754
Fragments arranged by size:
3133 2754 32 30 30 2
HincII GTy'rAC
Cuts at: 897 1811 3020 897
Size: 914 1209 3858
Fragments arranged by size:
3858 1209 914
KasI G'GCGC_C
Cuts at: 123 3411 3659 123
Size: 3288 248 2445
Fragments arranged by size:
3288 2445 248
KpnI G_GTAC'C
Cuts at: 3001 3001
Size: 5981
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1352 2279 2561 4740 4924 1247
Size: 105 927 282 2179 184 2304
Fragments arranged by size:
2304 2179 927 282 184 105
MscI TGG'CCA
Cuts at: 250 2404 2563 3742 250
Size: 2154 159 1179 2489
Fragments arranged by size:
2489 2154 1179 159
MslI CAynn'nnrTG
Cuts at: 547 1319 1839 1841 2056 2569 3970 4097 547
Size: 772 520 2 215 513 1401 127 2431
Fragments arranged by size:
2431 1401 772 520 513 215 127 2
MspA1I CmG'CkG
Cuts at: 1621 1784 1964 2415 2751 2849 3092 3406 3766
Size: 163 180 451 336 98 243 314 360
Cuts at: 3766 4550 4795 1621
Size: 784 245 2807
Fragments arranged by size:
2807 784 451 360 336 314 245 243
180 163 98
NaeI GCC'GGC
Cuts at: 2695 4162 2695
Size: 1467 4514
NarI GG'CG_CC
Cuts at: 124 3412 3660 124
Size: 3288 248 2445
Fragments arranged by size:
3288 2445 248
NcoI C'CATG_G
Cuts at: 209 209
Size: 5981
NdeI CA'TA_TG
Cuts at: 1694 1694
Size: 5981
NgoMIV G'CCGG_C
Cuts at: 2693 4160 2693
Size: 1467 4514
NspI r_CATG'y
Cuts at: 687 1461 2178 2190 2367 3034 3534 5137 687
Size: 774 717 12 177 667 500 1603 1531
Fragments arranged by size:
1603 1531 774 717 667 500 177 12
PasI CC'CwG_GG
Cuts at: 1848 1884 1848
Size: 36 5945
PciI A'CATG_T
Cuts at: 2186 5133 2186
Size: 2947 3034
PflMI CCAn_nnn'nTGG
Cuts at: 870 1419 2640 870
Size: 549 1221 4211
Fragments arranged by size:
4211 1221 549
PfoI T'CCnGG_A
Cuts at: 728 1376 1724 2270 2384 728
Size: 648 348 546 114 4325
Fragments arranged by size:
4325 648 546 348 114
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PmlI CAC'GTG
Cuts at: 1287 1287
Size: 5981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1896 4427 4459 1864
Size: 32 2531 32 3386
Fragments arranged by size:
3386 2531 32 32
PpuMI rG'GwC_Cy
Cuts at: 453 702 453
Size: 249 5732
PshAI GACnn'nnGTC
Cuts at: 399 399
Size: 5981
PstI C_TGCA'G
Cuts at: 169 348 2420 3028 3713 169
Size: 179 2072 608 685 2437
Fragments arranged by size:
2437 2072 685 608 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5981
PvuII CAG'CTG
Cuts at: 1621 1784 2415 3406 3766 1621
Size: 163 631 991 360 3836
Fragments arranged by size:
3836 991 631 360 163
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5981
SacI G_AGCT'C
Cuts at: 2995 2995
Size: 5981
SacII CC_GC'GG
Cuts at: 2752 2850 2752
Size: 98 5883
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SanDI GG'GwC_CC
Cuts at: 702 702
Size: 5981
SapI GCTCTTCn'nnn_
Cuts at: 1759 2212 4004 4214 1759
Size: 453 1792 210 3526
Fragments arranged by size:
3526 1792 453 210
SbfI CC_TGCA'GG
Cuts at: 3028 3028
Size: 5981
ScaI AGT'ACT
Cuts at: 1879 2293 5498 1879
Size: 414 3205 2362
Fragments arranged by size:
3205 2362 414
SfcI C'TryA_G
Cuts at: 33 165 344 554 2416 3024 3709 4677 4868
Size: 132 179 210 1862 608 685 968 191
Cuts at: 4868 33
Size: 1146
Fragments arranged by size:
1862 1146 968 685 608 210 191 179
132
SfoI GGC'GCC
Cuts at: 125 3413 3661 125
Size: 3288 248 2445
Fragments arranged by size:
3288 2445 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SgrAI Cr'CCGG_yG
Cuts at: 2693 2693
Size: 5981
SmaI CCC'GGG
Cuts at: 700 3003 700
Size: 2303 3678
SmlI C'TyrA_G
Cuts at: 180 718 1462 3136 4051 4765 5027 180
Size: 538 744 1674 915 714 262 1134
Fragments arranged by size:
1674 1134 915 744 714 538 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 687 2367 3034 3534 687
Size: 1680 667 500 3134
Fragments arranged by size:
3134 1680 667 500
SspI AAT'ATT
Cuts at: 2520 2520
Size: 5981
StuI AGG'CCT
Cuts at: 1607 2504 1607
Size: 897 5084
StyI C'CwwG_G
Cuts at: 209 293 371 662 2345 2477 3115 3469 209
Size: 84 78 291 1683 132 638 354 2721
Fragments arranged by size:
2721 1683 638 354 291 132 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1853 2662 3564 3697 4156 4365 1853
Size: 809 902 133 459 209 3469
Fragments arranged by size:
3469 902 809 459 209 133
TatI w'GTAC_w
Cuts at: 1877 2291 5496 1877
Size: 414 3205 2362
Fragments arranged by size:
3205 2362 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 2519 2551 5498 5530 2110
Size: 32 377 32 2947 32 2561
Fragments arranged by size:
2947 2561 377 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3778 3778
Size: 5981
XbaI T'CTAG_A
Cuts at: 3012 3012
Size: 5981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 2070 3476 300
Size: 1770 1406 2805
Fragments arranged by size:
2805 1770 1406
XmaI C'CCGG_G
Cuts at: 698 3001 698
Size: 2303 3678
XmnI GAAnn'nnTTC
Cuts at: 574 574
Size: 5981
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI ApaI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BspEI BsrGI BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PsiI PspOMI
PspXI PsrI SexAI SfiI SnaBI SrfI SwaI XhoI
ZraI