Restriction map for pF1KB3287
 (Linear) (Six-base) MAPSORT of: KIBB3287.seq  Check: 5279  from: 1  to: 2868



KIBB3287.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1312    2356    2868 
   Size:        706     606    1044     512

  Fragments arranged by size: 

                1044     706     606     512
 
AflIII A'CryG_T

Cuts at:      0    2078    2868 
   Size:       2078     790
 
AhdI GACnn_n'nnGTC

Cuts at:      0     564    2868 
   Size:        564    2304
 
AleI CACnn'nnGTG

Cuts at:      0    1733    2868 
   Size:       1733    1135
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1966    2868 
   Size:       1966     902
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1756    1788    2868 
   Size:       1756      32    1080

  Fragments arranged by size: 

                1756    1080      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     963    1790    2712    2790    2868 
   Size:        963     827     922      78      78

  Fragments arranged by size: 

                 963     922     827      78      78
 
ApoI r'AATT_y

Cuts at:      0    1090    2040    2142    2868 
   Size:       1090     950     102     726

  Fragments arranged by size: 

                1090     950     726     102
 
AscI GG'CGCG_CC

Cuts at:      0    2592    2868 
   Size:       2592     276
 
AvaI C'yCGr_G

Cuts at:      0     590    1426    2626    2767    2863    2868 
   Size:        590     836    1200     141      96       5

  Fragments arranged by size: 

                1200     836     590     141      96       5
 
BamHI G'GATC_C

Cuts at:      0     455    2868 
   Size:        455    2413
 
BanI G'GyrC_C

Cuts at:      0      15    1030    1438    2868 
   Size:         15    1015     408    1430

  Fragments arranged by size: 

                1430    1015     408      15
 
BanII G_rGCy'C

Cuts at:      0    1825    2693    2864    2868 
   Size:       1825     868     171       4

  Fragments arranged by size: 

                1825     868     171       4
 
BbeI G_GCGC'C

Cuts at:      0      19    2868 
   Size:         19    2849
 
BbvCI CC'TCA_GC

Cuts at:      0    1192    2868 
   Size:       1192    1676
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2244    2278    2786    2820    2868 
   Size:       2244      34     508      34      48

  Fragments arranged by size: 

                2244     508      48      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2584    2868 
   Size:       2584     284
 
BclI T'GATC_A

Cuts at:      0    1889    2868 
   Size:       1889     979
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1666    1918    2613    2734    2868 
   Size:       1666     252     695     121     134

  Fragments arranged by size: 

                1666     695     252     134     121
 
BglII A'GATC_T

Cuts at:      0    1016    1482    2868 
   Size:       1016     466    1386

  Fragments arranged by size: 

                1386    1016     466
 
BlpI GC'TnA_GC

Cuts at:      0    2659    2868 
   Size:       2659     209
 
Bme1580I G_kGCm'C

Cuts at:      0     890    1740    1955    2368    2650    2799    2868 
   Size:        890     850     215     413     282     149      69

  Fragments arranged by size: 

                 890     850     413     282     215     149      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     905    2868 
   Size:        905    1963
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     144    2868 
   Size:        144    2724
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     739     763    2004    2287    2347    2851    2868 
   Size:        291     448      24    1241     283      60     504      17

  Fragments arranged by size: 

                1241     504     448     291     283      60      24      17
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1192    2868 
   Size:       1192    1676
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     595    1339    2868 
   Size:         93     502     744    1529

  Fragments arranged by size: 

                1529     744     502      93
 
BsaAI yAC'GTr

Cuts at:      0    1179    2868 
   Size:       1179    1689
 
BsaBI GATnn'nnATC

Cuts at:      0    1894    2868 
   Size:       1894     974
 
BsaHI Gr'CG_yC

Cuts at:      0      16    1637    1705    1868    2868 
   Size:         16    1621      68     163    1000

  Fragments arranged by size: 

                1621    1000     163      68      16
 
BsaWI w'CCGG_w

Cuts at:      0     968    1797    2868 
   Size:        968     829    1071

  Fragments arranged by size: 

                1071     968     829
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1756    1786    2002    2032    2868 
   Size:       1756      30     216      30     836

  Fragments arranged by size: 

                1756     836     216      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     598     900    2134    2644    2836    2868 
   Size:        385     213     302    1234     510     192      32

  Fragments arranged by size: 

                1234     510     385     302     213     192      32
 
BseYI C'CCAG_C

Cuts at:      0     174     300     421     451    2095    2868 
   Size:        174     126     121      30    1644     773

  Fragments arranged by size: 

                1644     773     174     126     121      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     459     976    1957    2730    2868 
   Size:        129     330     517     981     773     138

  Fragments arranged by size: 

                 981     773     517     330     138     129
 
BsiEI CG_ry'CG

Cuts at:      0    1802    2641    2803    2868 
   Size:       1802     839     162      65

  Fragments arranged by size: 

                1802     839     162      65
 
BsiHKAI G_wGCw'C

Cuts at:      0    2116    2868 
   Size:       2116     752
 
BsmI GAATG_Cn'

Cuts at:      0     514    1568    1879    2868 
   Size:        514    1054     311     989

  Fragments arranged by size: 

                1054     989     514     311
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      15    2868 
   Size:         15    2853
 
Bsp1286I G_dGCh'C

Cuts at:      0     890    1740    1825    1955    2116    2368    2650    2693 
   Size:        890     850      85     130     161     252     282      43

Cuts at:   2693    2799    2864    2868 
   Size:        106      65       4

  Fragments arranged by size: 

                 890     850     282     252     161     130     106      85
                  65      43       4
 
BspHI T'CATG_A

Cuts at:      0    1040    1550    2868 
   Size:       1040     510    1318

  Fragments arranged by size: 

                1318    1040     510
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1058    2868 
   Size:       1058    1810
 
BsrBI CCG'CTC

Cuts at:      0     123     606    2868 
   Size:        123     483    2262

  Fragments arranged by size: 

                2262     483     123
 
BsrDI GCAATG_nn'

Cuts at:      0    1326    2868 
   Size:       1326    1542
 
BsrFI r'CCGG_y

Cuts at:      0    1078    1745    2585    2868 
   Size:       1078     667     840     283

  Fragments arranged by size: 

                1078     840     667     283
 
BssHII G'CGCG_C

Cuts at:      0    2592    2868 
   Size:       2592     276
 
BssSI C'ACGA_G

Cuts at:      0      43    2868 
   Size:         43    2825
 
BstEII G'GTnAC_C

Cuts at:      0    2535    2868 
   Size:       2535     333
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138     270    1269    2463    2868 
   Size:        138     132     999    1194     405

  Fragments arranged by size: 

                1194     999     405     138     132
 
BstYI r'GATC_y

Cuts at:      0     254     455     617    1016    1370    1391    1482    2330 
   Size:        254     201     162     399     354      21      91     848

Cuts at:   2330    2868 
   Size:        538

  Fragments arranged by size: 

                 848     538     399     354     254     201     162      91
                  21
 
BtgI C'CryG_G

Cuts at:      0     101    2641    2739    2868 
   Size:        101    2540      98     129

  Fragments arranged by size: 

                2540     129     101      98
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1815    2868 
   Size:       1815    1053
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     312     347    2050    2085    2868 
   Size:        312      35    1703      35     783

  Fragments arranged by size: 

                1703     783     312      35      35
 
DraI TTT'AAA

Cuts at:      0     518    2868 
   Size:        518    2350
 
EaeI y'GGCC_r

Cuts at:      0     140    1080    1167    1667    1910    2294    2453    2800 
   Size:        140     940      87     500     243     384     159     347

Cuts at:   2800    2868 
   Size:         68

  Fragments arranged by size: 

                 940     500     384     347     243     159     140      87
                  68
 
EagI C'GGCC_G

Cuts at:      0    2800    2868 
   Size:       2800      68
 
EarI CTCTTCn'nnn_

Cuts at:      0    1243    1651    1690    2104    2868 
   Size:       1243     408      39     414     764

  Fragments arranged by size: 

                1243     764     414     408      39
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1384    2868 
   Size:       1384    1484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     706     739     763    1312    2004    2287    2347 
   Size:        291     415      33      24     549     692     283      60

Cuts at:   2347    2356    2851    2868 
   Size:          9     495      17

  Fragments arranged by size: 

                 692     549     495     415     291     283      60      33
                  24      17       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262     685    2563    2868 
   Size:        262     423    1878     305

  Fragments arranged by size: 

                1878     423     305     262
 
EcoO109I rG'GnC_Cy

Cuts at:      0     345     594    1188    2223    2787    2868 
   Size:        345     249     594    1035     564      81

  Fragments arranged by size: 

                1035     594     564     345     249      81
 
EcoRI G'AATT_C

Cuts at:      0    1090    2142    2868 
   Size:       1090    1052     726

  Fragments arranged by size: 

                1090    1052     726
 
FspI TGC'GCA

Cuts at:      0    1054    2548    2868 
   Size:       1054    1494     320

  Fragments arranged by size: 

                1494    1054     320
 
HaeII r_GCGC'y

Cuts at:      0      19    2637    2868 
   Size:         19    2618     231

  Fragments arranged by size: 

                2618     231      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     550     582    2868 
   Size:        550      32    2286

  Fragments arranged by size: 

                2286     550      32
 
HincII GTy'rAC

Cuts at:      0     789    1703    2868 
   Size:        789     914    1165

  Fragments arranged by size: 

                1165     914     789
 
KasI G'GCGC_C

Cuts at:      0      15    2868 
   Size:         15    2853
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1139    1244    2171    2453    2868 
   Size:       1139     105     927     282     415

  Fragments arranged by size: 

                1139     927     415     282     105
 
MscI TGG'CCA

Cuts at:      0     142    2296    2455    2868 
   Size:        142    2154     159     413

  Fragments arranged by size: 

                2154     413     159     142
 
MslI CAynn'nnrTG

Cuts at:      0     439    1211    1731    1733    1948    2461    2868 
   Size:        439     772     520       2     215     513     407

  Fragments arranged by size: 

                 772     520     513     439     407     215       2
 
MspA1I CmG'CkG

Cuts at:      0    1513    1676    1856    2307    2643    2741    2868 
   Size:       1513     163     180     451     336      98     127

  Fragments arranged by size: 

                1513     451     336     180     163     127      98
 
NaeI GCC'GGC

Cuts at:      0    2587    2868 
   Size:       2587     281
 
NarI GG'CG_CC

Cuts at:      0      16    2868 
   Size:         16    2852
 
NcoI C'CATG_G

Cuts at:      0     101    2868 
   Size:        101    2767
 
NdeI CA'TA_TG

Cuts at:      0    1586    2868 
   Size:       1586    1282
 
NgoMIV G'CCGG_C

Cuts at:      0    2585    2868 
   Size:       2585     283
 
NspI r_CATG'y

Cuts at:      0     579    1353    2070    2082    2259    2868 
   Size:        579     774     717      12     177     609

  Fragments arranged by size: 

                 774     717     609     579     177      12
 
PasI CC'CwG_GG

Cuts at:      0    1740    1776    2868 
   Size:       1740      36    1092

  Fragments arranged by size: 

                1740    1092      36
 
PciI A'CATG_T

Cuts at:      0    2078    2868 
   Size:       2078     790
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     762    1311    2532    2868 
   Size:        762     549    1221     336

  Fragments arranged by size: 

                1221     762     549     336
 
PfoI T'CCnGG_A

Cuts at:      0     620    1268    1616    2162    2276    2868 
   Size:        620     648     348     546     114     592

  Fragments arranged by size: 

                 648     620     592     546     348     114
 
PmlI CAC'GTG

Cuts at:      0    1179    2868 
   Size:       1179    1689
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1756    1788    2868 
   Size:       1756      32    1080

  Fragments arranged by size: 

                1756    1080      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     345     594    2868 
   Size:        345     249    2274

  Fragments arranged by size: 

                2274     345     249
 
PshAI GACnn'nnGTC

Cuts at:      0     291    2868 
   Size:        291    2577
 
PstI C_TGCA'G

Cuts at:      0      61     240    2312    2868 
   Size:         61     179    2072     556

  Fragments arranged by size: 

                2072     556     179      61
 
PvuII CAG'CTG

Cuts at:      0    1513    1676    2307    2868 
   Size:       1513     163     631     561

  Fragments arranged by size: 

                1513     631     561     163
 
SacII CC_GC'GG

Cuts at:      0    2644    2742    2868 
   Size:       2644      98     126

  Fragments arranged by size: 

                2644     126      98
 
SanDI GG'GwC_CC

Cuts at:      0     594    2868 
   Size:        594    2274
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1651    2104    2868 
   Size:       1651     453     764

  Fragments arranged by size: 

                1651     764     453
 
ScaI AGT'ACT

Cuts at:      0    1771    2185    2868 
   Size:       1771     414     683

  Fragments arranged by size: 

                1771     683     414
 
SfcI C'TryA_G

Cuts at:      0      57     236     446    2308    2868 
   Size:         57     179     210    1862     560

  Fragments arranged by size: 

                1862     560     210     179      57
 
SfoI GGC'GCC

Cuts at:      0      17    2868 
   Size:         17    2851
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2585    2868 
   Size:       2585     283
 
SmaI CCC'GGG

Cuts at:      0     592    2868 
   Size:        592    2276
 
SmlI C'TyrA_G

Cuts at:      0      72     610    1354    2868 
   Size:         72     538     744    1514

  Fragments arranged by size: 

                1514     744     538      72
 
SphI G_CATG'C

Cuts at:      0     579    2259    2868 
   Size:        579    1680     609

  Fragments arranged by size: 

                1680     609     579
 
SspI AAT'ATT

Cuts at:      0    2412    2868 
   Size:       2412     456
 
StuI AGG'CCT

Cuts at:      0    1499    2396    2868 
   Size:       1499     897     472

  Fragments arranged by size: 

                1499     897     472
 
StyI C'CwwG_G

Cuts at:      0     101     185     263     554    2237    2369    2868 
   Size:        101      84      78     291    1683     132     499

  Fragments arranged by size: 

                1683     499     291     132     101      84      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1745    2554    2868 
   Size:       1745     809     314

  Fragments arranged by size: 

                1745     809     314
 
TatI w'GTAC_w

Cuts at:      0    1769    2183    2868 
   Size:       1769     414     685

  Fragments arranged by size: 

                1769     685     414
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2002    2034    2411    2443    2868 
   Size:       2002      32     377      32     425

  Fragments arranged by size: 

                2002     425     377      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192    1962    2868 
   Size:        192    1770     906

  Fragments arranged by size: 

                1770     906     192
 
XmaI C'CCGG_G

Cuts at:      0     590    2868 
   Size:        590    2278
 
XmnI GAAnn'nnTTC

Cuts at:      0     466    2868 
   Size:        466    2402
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApoI
     AscI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstEII
    BstXI    BstYI     BtgI    BtgZI    CspCI     DraI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PstI    PvuII    SacII
    SanDI     SapI     ScaI     SfcI     SfoI    SgrAI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     ApaI    ApaLI     AseI    AsiSI    AvrII     BaeI     BbsI    BfrBI
    BmgBI     BmtI     BsaI    BsiWI    BspEI    BsrGI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtsI     ClaI   DraIII     DrdI  EcoICRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SbfI    SexAI
     SfiI     SgfI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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