Restriction map for pF1KB3283
 (Linear) (Six-base) MAPSORT of: KIBB3283.seq  Check: 711  from: 1  to: 3567



KIBB3283.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     490     716    1679    3500    3567 
   Size:        490     226     963    1821      67

  Fragments arranged by size: 

                1821     963     490     226      67
 
AccI GT'mk_AC

Cuts at:      0     560    3567 
   Size:        560    3007
 
Acc65I G'GTAC_C

Cuts at:      0     908    3567 
   Size:        908    2659
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     579    1156    1429    3567 
   Size:         43     536     577     273    2138

  Fragments arranged by size: 

                2138     577     536     273      43
 
AflIII A'CryG_T

Cuts at:      0    3164    3567 
   Size:       3164     403
 
AleI CACnn'nnGTG

Cuts at:      0     936    3567 
   Size:        936    2631
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2044    3067    3567 
   Size:       2044    1023     500

  Fragments arranged by size: 

                2044    1023     500
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2408    2440    3567 
   Size:       2408      32    1127

  Fragments arranged by size: 

                2408    1127      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     817    1109    1312    1891    2700    3387    3567 
   Size:        817     292     203     579     809     687     180

  Fragments arranged by size: 

                 817     809     687     579     292     203     180
 
ApaI G_GGCC'C

Cuts at:      0     205     241    1230    1740    2100    2255    3567 
   Size:        205      36     989     510     360     155    1312

  Fragments arranged by size: 

                1312     989     510     360     205     155      36
 
ApaLI G'TGCA_C

Cuts at:      0    1941    3567 
   Size:       1941    1626
 
AvaI C'yCGr_G

Cuts at:      0      95    1249    1380    1437    2087    2101    2463    3567 
   Size:         95    1154     131      57     650      14     362    1104

  Fragments arranged by size: 

                1154    1104     650     362     131      95      57      14
            
 
BamHI G'GATC_C

Cuts at:      0     627    2627    3567 
   Size:        627    2000     940

  Fragments arranged by size: 

                2000     940     627
 
BanI G'GyrC_C

Cuts at:      0      28     474     704     908     938    1051    3080    3232 
   Size:         28     446     230     204      30     113    2029     152

Cuts at:   3232    3247    3287    3354    3567 
   Size:         15      40      67     213

  Fragments arranged by size: 

                2029     446     230     213     204     152     113      67
                  40      30      28      15
 
BanII G_rGCy'C

Cuts at:      0      83     205     241     465     659    1230    1740    2100 
   Size:         83     122      36     224     194     571     510     360

Cuts at:   2100    2255    2552    2707    2800    2957    2986    3128    3369 
   Size:        155     297     155      93     157      29     142     241

Cuts at:   3369    3567 
   Size:        198

  Fragments arranged by size: 

                 571     510     360     297     241     224     198     194
                 157     155     155     142     122      93      83      36
                  29
 
BbeI G_GCGC'C

Cuts at:      0    3236    3251    3291    3567 
   Size:       3236      15      40     276

  Fragments arranged by size: 

                3236     276      40      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52    1147    1595    3567 
   Size:         52    1095     448    1972

  Fragments arranged by size: 

                1972    1095     448      52
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1641    1675    3567 
   Size:       1641      34    1892

  Fragments arranged by size: 

                1892    1641      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     724    1037    3078    3567 
   Size:        724     313    2041     489

  Fragments arranged by size: 

                2041     724     489     313
 
BlpI GC'TnA_GC

Cuts at:      0    1648    2017    2701    3449    3567 
   Size:       1648     369     684     748     118

  Fragments arranged by size: 

                1648     748     684     369     118
 
Bme1580I G_kGCm'C

Cuts at:      0      31     205     241     346     479     587     707     824 
   Size:         31     174      36     105     133     108     120     117

Cuts at:    824     943    1230    1503    1529    1740    1945    1961    2066 
   Size:        119     287     273      26     211     205      16     105

Cuts at:   2066    2100    2255    2679    3090    3177    3220    3567 
   Size:         34     155     424     411      87      43     347

  Fragments arranged by size: 

                 424     411     347     287     273     211     205     174
                 155     133     120     119     117     108     105     105
                  87      43      36      34      31      26      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1901    1980    2357    3522    3567 
   Size:       1901      79     377    1165      45

  Fragments arranged by size: 

                1901    1165     377      79      45
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1577    2778    3567 
   Size:       1577    1201     789

  Fragments arranged by size: 

                1577    1201     789
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     475    1063    1414    1532    1590    1980    2483 
   Size:        460      15     588     351     118      58     390     503

Cuts at:   2483    2616    2971    3190    3259    3313    3567 
   Size:        133     355     219      69      54     254

  Fragments arranged by size: 

                 588     503     460     390     355     351     254     219
                 133     118      69      58      54      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     160     371     600     950    2771    3567 
   Size:        160     211     229     350    1821     796

  Fragments arranged by size: 

                1821     796     350     229     211     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    3567 
   Size:        376    3191
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     436    2197    2215    3137    3424    3567 
   Size:        436    1761      18     922     287     143

  Fragments arranged by size: 

                1761     922     436     287     143      18
 
BsaAI yAC'GTr

Cuts at:      0    3165    3567 
   Size:       3165     402
 
BsaHI Gr'CG_yC

Cuts at:      0     155    3182    3233    3248    3288    3567 
   Size:        155    3027      51      15      40     279

  Fragments arranged by size: 

                3027     279     155      51      40      15
 
BsaWI w'CCGG_w

Cuts at:      0    1293    2877    3567 
   Size:       1293    1584     690

  Fragments arranged by size: 

                1584    1293     690
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2675    2705    3332    3362    3406    3436    3567 
   Size:       2675      30     627      30      44      30     131

  Fragments arranged by size: 

                2675     627     131      44      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1216    1535    1951    2215    3313    3567 
   Size:       1216     319     416     264    1098     254

  Fragments arranged by size: 

                1216    1098     416     319     264     254
 
BseYI C'CCAG_C

Cuts at:      0      13     270     883     915     965    1072    1117    1327 
   Size:         13     257     613      32      50     107      45     210

Cuts at:   1327    1739    2226    2552    2720    2725    2957    3185    3469 
   Size:        412     487     326     168       5     232     228     284

Cuts at:   3469    3567 
   Size:         98

  Fragments arranged by size: 

                 613     487     412     326     284     257     232     228
                 210     168     107      98      50      45      32      13
                   5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     727    2713    2797    3400    3567 
   Size:         91     636    1986      84     603     167

  Fragments arranged by size: 

                1986     636     603     167      91      84
 
BsiEI CG_ry'CG

Cuts at:      0    3224    3567 
   Size:       3224     343
 
BsiHKAI G_wGCw'C

Cuts at:      0     166     465    1945    2023    2594    3397    3567 
   Size:        166     299    1480      78     571     803     170

  Fragments arranged by size: 

                1480     803     571     299     170     166      78
 
BsmI GAATG_Cn'

Cuts at:      0     325     635    1784    3567 
   Size:        325     310    1149    1783

  Fragments arranged by size: 

                1783    1149     325     310
 
Bsp1286I G_dGCh'C

Cuts at:      0      31      83     166     205     241     346     465     479 
   Size:         31      52      83      39      36     105     119      14

Cuts at:    479     587     659     707     824     943    1230    1503    1529 
   Size:        108      72      48     117     119     287     273      26

Cuts at:   1529    1740    1945    1961    2023    2066    2100    2255    2552 
   Size:        211     205      16      62      43      34     155     297

Cuts at:   2552    2594    2679    2707    2800    2957    2986    3090    3128 
   Size:         42      85      28      93     157      29     104      38

Cuts at:   3128    3177    3220    3369    3397    3567 
   Size:         49      43     149      28     170

  Fragments arranged by size: 

                 297     287     273     211     205     170     157     155
                 149     119     119     117     108     105     104      93
                  85      83      72      62      52      49      48      43
                  43      42      39      38      36      34      31      29
                  28      28      26      16      14
 
BspEI T'CCGG_A

Cuts at:      0    2877    3567 
   Size:       2877     690
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     490     716    1679    3500    3567 
   Size:        490     226     963    1821      67

  Fragments arranged by size: 

                1821     963     490     226      67
 
BsrBI CCG'CTC

Cuts at:      0     795    1719    3567 
   Size:        795     924    1848

  Fragments arranged by size: 

                1848     924     795
 
BsrDI GCAATG_nn'

Cuts at:      0    1271    2033    2172    2638    3567 
   Size:       1271     762     139     466     929

  Fragments arranged by size: 

                1271     929     762     466     139
 
BsrFI r'CCGG_y

Cuts at:      0     773    1209    1643    1856    3567 
   Size:        773     436     434     213    1711

  Fragments arranged by size: 

                1711     773     436     434     213
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1344    1805    3567 
   Size:       1344     461    1762

  Fragments arranged by size: 

                1762    1344     461
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     176     453     533     754    1510    2388    2733    2847 
   Size:        176     277      80     221     756     878     345     114

Cuts at:   2847    3567 
   Size:        720

  Fragments arranged by size: 

                 878     756     720     345     277     221     176     114
                  80
 
BstYI r'GATC_y

Cuts at:      0     449     627    1008    2423    2627    3567 
   Size:        449     178     381    1415     204     940

  Fragments arranged by size: 

                1415     940     449     381     204     178
 
BstZ17I GTA'TAC

Cuts at:      0     561    3567 
   Size:        561    3006
 
Bsu36I CC'TnA_GG

Cuts at:      0    1193    3567 
   Size:       1193    2374
 
BtgI C'CryG_G

Cuts at:      0     111    1812    1931    2294    2895    3542    3567 
   Size:        111    1701     119     363     601     647      25

  Fragments arranged by size: 

                1701     647     601     363     119     111      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     908    1851    3567 
   Size:        908     943    1716

  Fragments arranged by size: 

                1716     943     908
 
BtsI GCAGTG_nn'

Cuts at:      0     423     739    1310    2021    2064    3067    3536    3567 
   Size:        423     316     571     711      43    1003     469      31

  Fragments arranged by size: 

                1003     711     571     469     423     316      43      31
            
 
ClaI AT'CG_AT

Cuts at:      0    1357    3567 
   Size:       1357    2210
 
DraIII CAC_nnn'GTG

Cuts at:      0    3158    3378    3567 
   Size:       3158     220     189

  Fragments arranged by size: 

                3158     220     189
 
EaeI y'GGCC_r

Cuts at:      0     384     771    1288    1416    2244    3029    3221    3539 
   Size:        384     387     517     128     828     785     192     318

Cuts at:   3539    3567 
   Size:         28

  Fragments arranged by size: 

                 828     785     517     387     384     318     192     128
                  28
 
EagI C'GGCC_G

Cuts at:      0    3221    3567 
   Size:       3221     346
 
EarI CTCTTCn'nnn_

Cuts at:      0     802    1825    2587    3567 
   Size:        802    1023     762     980

  Fragments arranged by size: 

                1023     980     802     762
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3184    3567 
   Size:       3184     383
 
EcoICRI GAG'CTC

Cuts at:      0     463    3567 
   Size:        463    3104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     460     475     579    1063    1156    1414    1429 
   Size:         43     417      15     104     484      93     258      15

Cuts at:   1429    1532    1590    1980    2483    2616    2971    3190    3259 
   Size:        103      58     390     503     133     355     219      69

Cuts at:   3259    3313    3567 
   Size:         54     254

  Fragments arranged by size: 

                 503     484     417     390     355     258     254     219
                 133     104     103      93      69      58      54      43
                  15      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     147    1304    1565    2522    2584    3295    3328    3567 
   Size:        147    1157     261     957      62     711      33     239

  Fragments arranged by size: 

                1157     957     711     261     239     147      62      33
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     202     237     238    1038    1106    1227    1405 
   Size:        201       1      35       1     800      68     121     178

Cuts at:   1405    1434    1725    2097    2251    2252    2478    3333    3384 
   Size:         29     291     372     154       1     226     855      51

Cuts at:   3384    3466    3567 
   Size:         82     101

  Fragments arranged by size: 

                 855     800     372     291     226     201     178     154
                 121     101      82      68      51      35      29       1
                   1       1
 
EcoRV GAT'ATC

Cuts at:      0    2055    3567 
   Size:       2055    1512
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     675    3118    3567 
   Size:        675    2443     449

  Fragments arranged by size: 

                2443     675     449
 
FspI TGC'GCA

Cuts at:      0    2473    3567 
   Size:       2473    1094
 
HaeII r_GCGC'y

Cuts at:      0    2701    3236    3251    3291    3567 
   Size:       2701     535      15      40     276

  Fragments arranged by size: 

                2701     535     276      40      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1363    1395    2652    2684    2782    2814    3567 
   Size:       1363      32    1257      32      98      32     753

  Fragments arranged by size: 

                1363    1257     753      98      32      32      32
 
HincII GTy'rAC

Cuts at:      0     264    3567 
   Size:        264    3303
 
KasI G'GCGC_C

Cuts at:      0    3232    3247    3287    3567 
   Size:       3232      15      40     280

  Fragments arranged by size: 

                3232     280      40      15
 
KpnI G_GTAC'C

Cuts at:      0     912    3567 
   Size:        912    2655
 
MscI TGG'CCA

Cuts at:      0     386    1418    3567 
   Size:        386    1032    2149

  Fragments arranged by size: 

                2149    1032     386
 
MslI CAynn'nnrTG

Cuts at:      0     380     527     936    2026    3049    3567 
   Size:        380     147     409    1090    1023     518

  Fragments arranged by size: 

                1090    1023     518     409     380     147
 
MspA1I CmG'CkG

Cuts at:      0     120     321     667     915     969    1510    1743    1847 
   Size:        120     201     346     248      54     541     233     104

Cuts at:   1847    1933    2218    2296    3260    3567 
   Size:         86     285      78     964     307

  Fragments arranged by size: 

                 964     541     346     307     285     248     233     201
                 120     104      86      78      54
 
NaeI GCC'GGC

Cuts at:      0     775    1645    3567 
   Size:        775     870    1922

  Fragments arranged by size: 

                1922     870     775
 
NarI GG'CG_CC

Cuts at:      0    3233    3248    3288    3567 
   Size:       3233      15      40     279

  Fragments arranged by size: 

                3233     279      40      15
 
NcoI C'CATG_G

Cuts at:      0     111    1812    2895    3567 
   Size:        111    1701    1083     672

  Fragments arranged by size: 

                1701    1083     672     111
 
NgoMIV G'CCGG_C

Cuts at:      0     773    1643    3567 
   Size:        773     870    1924

  Fragments arranged by size: 

                1924     870     773
 
NspI r_CATG'y

Cuts at:      0     216    3058    3567 
   Size:        216    2842     509

  Fragments arranged by size: 

                2842     509     216
 
PasI CC'CwG_GG

Cuts at:      0     198     554    1103    1624    2439    2524    3567 
   Size:        198     356     549     521     815      85    1043

  Fragments arranged by size: 

                1043     815     549     521     356     198      85
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     321     895    2007    3567 
   Size:        321     574    1112    1560

  Fragments arranged by size: 

                1560    1112     574     321
 
PfoI T'CCnGG_A

Cuts at:      0     452    1430    2387    2426    2585    3567 
   Size:        452     978     957      39     159     982

  Fragments arranged by size: 

                 982     978     957     452     159      39
 
PmlI CAC'GTG

Cuts at:      0    3165    3567 
   Size:       3165     402
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1078    1110    3567 
   Size:       1078      32    2457

  Fragments arranged by size: 

                2457    1078      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1405    1434    3466    3567 
   Size:       1405      29    2032     101

  Fragments arranged by size: 

                2032    1405     101      29
 
PspOMI G'GGCC_C

Cuts at:      0     201     237    1226    1736    2096    2251    3567 
   Size:        201      36     989     510     360     155    1316

  Fragments arranged by size: 

                1316     989     510     360     201     155      36
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     892     924    3567 
   Size:        892      32    2643

  Fragments arranged by size: 

                2643     892      32
 
PstI C_TGCA'G

Cuts at:      0     713    1317    1580    1801    1852    2546    3363    3567 
   Size:        713     604     263     221      51     694     817     204

  Fragments arranged by size: 

                 817     713     694     604     263     221     204      51
            
 
PvuII CAG'CTG

Cuts at:      0     667     915    1510    3260    3567 
   Size:        667     248     595    1750     307

  Fragments arranged by size: 

                1750     667     595     307     248
 
SacI G_AGCT'C

Cuts at:      0     465    3567 
   Size:        465    3102
 
SacII CC_GC'GG

Cuts at:      0    1934    2297    3567 
   Size:       1934     363    1270

  Fragments arranged by size: 

                1934    1270     363
 
SanDI GG'GwC_CC

Cuts at:      0    3466    3567 
   Size:       3466     101
 
SapI GCTCTTCn'nnn_

Cuts at:      0     802    3567 
   Size:        802    2765
 
SbfI CC_TGCA'GG

Cuts at:      0    3363    3567 
   Size:       3363     204
 
ScaI AGT'ACT

Cuts at:      0    2884    3567 
   Size:       2884     683
 
SexAI A'CCwGG_T

Cuts at:      0    2033    3035    3374    3567 
   Size:       2033    1002     339     193

  Fragments arranged by size: 

                2033    1002     339     193
 
SfcI C'TryA_G

Cuts at:      0     709    1313    1576    1797    1848    2542    3359    3567 
   Size:        709     604     263     221      51     694     817     208

  Fragments arranged by size: 

                 817     709     694     604     263     221     208      51
            
 
SfoI GGC'GCC

Cuts at:      0    3234    3249    3289    3567 
   Size:       3234      15      40     278

  Fragments arranged by size: 

                3234     278      40      15
 
SmaI CCC'GGG

Cuts at:      0    1439    2089    2103    2465    3567 
   Size:       1439     650      14     362    1102

  Fragments arranged by size: 

                1439    1102     650     362      14
 
SmlI C'TyrA_G

Cuts at:      0      95     391    3567 
   Size:         95     296    3176

  Fragments arranged by size: 

                3176     296      95
 
SphI G_CATG'C

Cuts at:      0    3058    3567 
   Size:       3058     509
 
StuI AGG'CCT

Cuts at:      0     854    1093    1658    3110    3567 
   Size:        854     239     565    1452     457

  Fragments arranged by size: 

                1452     854     565     457     239
 
StyI C'CwwG_G

Cuts at:      0     111     206     659     903     934    1752    1812    2895 
   Size:        111      95     453     244      31     818      60    1083

Cuts at:   2895    3567 
   Size:        672

  Fragments arranged by size: 

                1083     818     672     453     244     111      95      60
                  31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462     979    1008    1706    1843    2191    3567 
   Size:        462     517      29     698     137     348    1376

  Fragments arranged by size: 

                1376     698     517     462     348     137      29
 
TatI w'GTAC_w

Cuts at:      0    2882    3567 
   Size:       2882     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1681    1713    2675    2707    3332    3364    3406    3438 
   Size:       1681      32     962      32     625      32      42      32

Cuts at:   3438    3567 
   Size:        129

  Fragments arranged by size: 

                1681     962     625     129      42      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3037    3567 
   Size:       3037     530
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2004    3074    3567 
   Size:       2004    1070     493

  Fragments arranged by size: 

                2004    1070     493
 
XhoI C'TCGA_G

Cuts at:      0      95    3567 
   Size:         95    3472
 
XmaI C'CCGG_G

Cuts at:      0    1437    2087    2101    2463    3567 
   Size:       1437     650      14     362    1104

  Fragments arranged by size: 

                1437    1104     650     362      14
 
XmnI GAAnn'nnTTC

Cuts at:      0    3097    3567 
   Size:       3097     470
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI    BsaBI    BsiWI    BsmBI    BspHI    BsrGI
   BssHII    BssSI    BstBI   BstEII    CspCI     DraI     DrdI    EcoRI
     FseI    FspAI  HindIII     HpaI     MfeI     MluI     MmeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI    PshAI
     PsiI    PspXI     PvuI    RsrII     SalI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     ZraI
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