Restriction map for
pF1KB3272
##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BbrPI BciVI BfrI BlnI BsiWI
BspHI BssHII Bst1107I BstBI BstXI
BstZ17I CpoI Eam1105I EcoNI EcoT22I
FseI KasI KpnI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 2012 TCCGGA T^CCGGA
Aor51HI 2222 AGCGCT AGC^GCT
Asp700I 1590 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1250 TGGCCA TGG^CCA
BglI 1376 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3263 AGATCT A^GATCT
BsaBI 1350 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2012 TCCGGA T^CCGGA
BsgI 1156 GTGCAG GTGCAG(16/14)
BsiEI 2094 CGGCCG CG[GA][CT]^CG
Bsp1407I 1442 TGTACA T^GTACA
BspDI 645 ATCGAT AT^CGAT
BspEI 2012 TCCGGA T^CCGGA
BspLU11I 1445 ACATGT A^CATGT
BsrGI 1442 TGTACA T^GTACA
BstAPI 2100 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 997 GGTGACC G^GT[ACGT]ACC
BstPI 997 GGTGACC G^GT[ACGT]ACC
ClaI 645 ATCGAT AT^CGAT
DraI 523 TTTAAA TTT^AAA
DraIII 2101 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2794 GACCTGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2094 CGGCCG C^GGCCG
EclXI 2094 CGGCCG C^GGCCG
Eco47III 2222 AGCGCT AGC^GCT
Eco52I 2094 CGGCCG C^GGCCG
EcoO65I 997 GGTGACC G^GT[ACGT]ACC
EcoRI 658 GAATTC G^AATTC
EcoRV 196 GATATC GAT^ATC
HaeII 2222 AGCGCT [GA]GCGC^[CT]
HindIII 1028 AAGCTT A^AGCTT
HpaI 358 GTTAAC GTT^AAC
KspI 1956 CCGCGG CCGC^GG
MamI 1350 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1250 TGGCCA TGG^CCA
MroI 2012 TCCGGA T^CCGGA
MscI 1250 TGGCCA TGG^CCA
NaeI 2096 GCCGGC GCC^GGC
NgoMIV 2096 GCCGGC G^CCGGC
PaeR7I 1188 CTCGAG C^TCGAG
PflMI 1928 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2946 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1956 CCGCGG CCGC^GG
SalI 2914 GTCGAC G^TCGAC
SapI 42 GAAGAGC GCTCTTC(1/4)
SexAI 2795 ACCTGGT A^CC[AT]GGT
SmlI 1188 CTCGAG C^T[CT][GA]AG
SspBI 1442 TGTACA T^GTACA
SspI 2553 AATATT AAT^ATT
StuI 1256 AGGCCT AGG^CCT
Van91I 1928 CCAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2666 CCACAGACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1188 CTCGAG C^TCGAG
XmnI 1590 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1150 GACGTC GACGT^C
2260 GACGTC
AcsI 658 GAATTC [GA]^AATT[CT]
3368 AAATTT
AcyI 1150 GACGTC G[GA]^CG[CT]C
2260 GACGTC
AflIII 983 ACACGT A^C[GA][CT]GT
1445 ACATGT
AlwNI 778 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
897 CAGCATCTG
AocI 2531 CCTTAGG CC^T[ACGT]AGG
2590 CCTCAGG
ApaI 1087 GGGCCC GGGCC^C
1374 GGGCCC
ApoI 658 GAATTC [GA]^AATT[CT]
3368 AAATTT
AviII 1046 TGCGCA TGC^GCA
3668 TGCGCA
BanI 1675 GGCACC G^G[CT][GA]CC
2377 GGTGCC
BcgI 2031 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
302 GCACTCAGTTCG
BclI 134 TGATCA T^GATCA
515 TGATCA
BfaI 2057 CTAG C^TAG
2503 CTAG
BsaAI 873 CACGTA [CT]AC^GT[GA]
984 CACGTA
BsaHI 1150 GACGTC G[GA]^CG[CT]C
2260 GACGTC
BsaI 1699 GAGACC GGTCTC(1/5)
2537 GGTCTC
BsaWI 2012 TCCGGA [AT]^CCGG[AT]
2652 TCCGGT
BsmI 402 GAATGC GAATGC(1/-1)
2166 GCATTC
BsrDI 1491 CATTGC GCAATG(2/0)
3671 GCAATG
BsrFI 1101 GCCGGT [GA]^CCGG[CT]
2096 GCCGGC
BssSI 887 CACGAG CACGAG(-5/-1)
1921 CACGAG
Bsu36I 2531 CCTTAGG CC^T[ACGT]AGG
2590 CCTCAGG
Cfr10I 1101 GCCGGT [GA]^CCGG[CT]
2096 GCCGGC
Eco81I 2531 CCTTAGG CC^T[ACGT]AGG
2590 CCTCAGG
FbaI 134 TGATCA T^GATCA
515 TGATCA
FspI 1046 TGCGCA TGC^GCA
3668 TGCGCA
Hin1I 1150 GACGTC G[GA]^CG[CT]C
2260 GACGTC
MaeI 2057 CTAG C^TAG
2503 CTAG
NcoI 1113 CCATGG C^CATGG
1772 CCATGG
PpuMI 1783 GGGACCC [GA]G^G[AT]CC[CT]
2113 GGGACCT
ScaI 2450 AGTACT AGT^ACT
2753 AGTACT
SmaI 1991 CCCGGG CCC^GGG
2407 CCCGGG
XmaCI 1991 CCCGGG C^CCGGG
2407 CCCGGG
XmaI 1991 CCCGGG C^CCGGG
2407 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaI AvaII BamHI
BanII BbsI BbvCI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII