Restriction map for
pF1KB3272
(Circular) (Six-base) MAPSORT of: pF1KB3272.seq Check: 9636 from: 1 to: 6908
pF1KB3272 6908 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 249 1979 249
Size: 1730 5178
AatII G_ACGT'C
Cuts at: 1262 2372 1262
Size: 1110 5798
AccI GT'mk_AC
Cuts at: 2912 3023 3062 3946 2912
Size: 111 39 884 5874
Fragments arranged by size:
5874 884 111 39
Acc65I G'GTAC_C
Cuts at: 3924 3924
Size: 6908
AclI AA'CG_TT
Cuts at: 6748 6748
Size: 6908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1605 1911 2142 3052 3628 4733 5165 5912 6180
Size: 306 231 910 576 1105 432 747 268
Cuts at: 6180 1605
Size: 2333
Fragments arranged by size:
2333 1105 910 747 576 432 306 268
231
AfeI AGC'GCT
Cuts at: 2332 2332
Size: 6908
AflIII A'CryG_T
Cuts at: 1091 1553 4260 5364 6341 1091
Size: 462 2707 1104 977 1658
Fragments arranged by size:
2707 1658 1104 977 462
AgeI A'CCGG_T
Cuts at: 5296 5296
Size: 6908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1687 1719 1687
Size: 32 6876
AlwNI CAG_nnn'CTG
Cuts at: 891 1010 4213 5780 891
Size: 119 3203 1567 2019
Fragments arranged by size:
3203 2019 1567 119
ApaI G_GGCC'C
Cuts at: 1199 1486 1199
Size: 287 6621
ApaLI G'TGCA_C
Cuts at: 5678 5678
Size: 6908
ApoI r'AATT_y
Cuts at: 766 3476 3912 6261 6517 766
Size: 2710 436 2349 256 1157
Fragments arranged by size:
2710 2349 1157 436 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
AvaI C'yCGr_G
Cuts at: 220 1296 1487 2099 2317 2515 3265 3928 220
Size: 1076 191 612 218 198 750 663 3200
Fragments arranged by size:
3200 1076 750 663 612 218 198 191
BamHI G'GATC_C
Cuts at: 1868 2388 2549 3933 1868
Size: 520 161 1384 4843
Fragments arranged by size:
4843 1384 520 161
BanI G'GyrC_C
Cuts at: 1783 2485 3924 4338 4586 4621 1783
Size: 702 1439 414 248 35 4070
Fragments arranged by size:
4070 1439 702 414 248 35
BanII G_rGCy'C
Cuts at: 171 698 885 1199 1486 2255 2324 2516 2741
Size: 527 187 314 287 769 69 192 225
Cuts at: 2741 2795 2897 3450 3922 4952 171
Size: 54 102 553 472 1030 2127
Fragments arranged by size:
2127 1030 769 553 527 472 314 287
225 192 187 102 69 54
BbeI G_GCGC'C
Cuts at: 4342 4590 4342
Size: 248 6660
BbsI GAAGACnn'nnnn_
Cuts at: 389 613 2636 2718 389
Size: 224 2023 82 4579
Fragments arranged by size:
4579 2023 224 82
BbvCI CC'TCA_GC
Cuts at: 424 3433 3715 424
Size: 3009 282 3617
Fragments arranged by size:
3617 3009 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 399 433 2128 2162 399
Size: 34 1695 34 5145
Fragments arranged by size:
5145 1695 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4806 5567 6908 4806
Size: 761 1341 4806
Fragments arranged by size:
4806 1341 761
BclI T'GATC_A
Cuts at: 242 623 4428 242
Size: 381 3805 2722
Fragments arranged by size:
3805 2722 381
BglI GCCn_nnn'nGGC
Cuts at: 1490 1490
Size: 6908
BglII A'GATC_T
Cuts at: 3371 4423 6460 3371
Size: 1052 2037 3819
Fragments arranged by size:
3819 2037 1052
BlpI GC'TnA_GC
Cuts at: 433 697 950 2470 4020 433
Size: 264 253 1520 1550 3321
Fragments arranged by size:
3321 1550 1520 264 253
Bme1580I G_kGCm'C
Cuts at: 1199 1486 1661 2015 2490 4533 4626 5682 1199
Size: 287 175 354 475 2043 93 1056 2425
Fragments arranged by size:
2425 2043 1056 475 354 287 175 93
BmrI ACTGGGnnnn_n'
Cuts at: 1300 2387 2874 4120 4201 4536 6680 1300
Size: 1087 487 1246 81 335 2144 1528
Fragments arranged by size:
2144 1528 1246 1087 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2604 5275 2604
Size: 2671 4237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 288 2347 2755 3697 3817 193
Size: 95 2059 408 942 120 3284
Fragments arranged by size:
3284 2059 942 408 120 95
Bpu10I CC'TnA_GC
Cuts at: 424 884 2059 3433 3715 424
Size: 460 1175 1374 282 3617
Fragments arranged by size:
3617 1374 1175 460 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4963 5455 5753 4084
Size: 879 492 298 5239
Fragments arranged by size:
5239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1801 2651 6612 1801
Size: 850 3961 2097
Fragments arranged by size:
3961 2097 850
BsaAI yAC'GTr
Cuts at: 983 1094 4261 983
Size: 111 3167 3630
Fragments arranged by size:
3630 3167 111
BsaBI GATnn'nnATC
Cuts at: 6 1462 4422 6459 6
Size: 1456 2960 2037 455
Fragments arranged by size:
2960 2037 1456 455
BsaHI Gr'CG_yC
Cuts at: 1259 2369 4339 4587 5289 6785 1259
Size: 1110 1970 248 702 1496 1382
Fragments arranged by size:
1970 1496 1382 1110 702 248
BsaWI w'CCGG_w
Cuts at: 2120 2760 4097 4618 5296 5570 5717 6128 2120
Size: 640 1337 521 678 274 147 411 2900
Fragments arranged by size:
2900 1337 678 640 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1060 1090 1687 1717 2488 2518 1060
Size: 30 597 30 771 30 5450
Fragments arranged by size:
5450 771 597 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1068 1339 1663 2620 2803 3690 3732 1068
Size: 271 324 957 183 887 42 4244
Fragments arranged by size:
4244 957 887 324 271 183 42
BseYI C'CCAG_C
Cuts at: 177 1187 1438 1479 2217 4333 5091 5668 6164
Size: 1010 251 41 738 2116 758 577 496
Cuts at: 6164 177
Size: 921
Fragments arranged by size:
2116 1010 921 758 738 577 496 251
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1285
Size: 6908
BsiEI CG_ry'CG
Cuts at: 104 2205 4450 4496 5704 104
Size: 2101 2245 46 1208 1308
Fragments arranged by size:
2245 2101 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 684 698 1331 2255 2324 2795 2897 3318 3922
Size: 14 633 924 69 471 102 421 604
Cuts at: 3922 4700 4890 5682 684
Size: 778 190 792 1910
Fragments arranged by size:
1910 924 792 778 633 604 471 421
190 102 69 14
BsmI GAATG_Cn'
Cuts at: 516 2274 516
Size: 1758 5150
BsmBI CGTCTCn'nnnn_
Cuts at: 244 1759 1957 2980 244
Size: 1515 198 1023 4172
Fragments arranged by size:
4172 1515 1023 198
Bsp1286I G_dGCh'C
Cuts at: 171 684 698 885 1199 1331 1486 1661 2015
Size: 513 14 187 314 132 155 175 354
Cuts at: 2015 2255 2324 2490 2516 2741 2795 2897 3318
Size: 240 69 166 26 225 54 102 421
Cuts at: 3318 3450 3922 4533 4626 4700 4890 4952 5682
Size: 132 472 611 93 74 190 62 730
Cuts at: 5682 171
Size: 1397
Fragments arranged by size:
1397 730 611 513 472 421 354 314
240 225 190 187 175 166 155 132
132 102 93 74 69 62 54 26
14
BspEI T'CCGG_A
Cuts at: 2120 6128 2120
Size: 4008 2900
BspHI T'CATG_A
Cuts at: 6084 6084
Size: 6908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 249 842 1397 1979 3958 4172 4474 4855 249
Size: 593 555 582 1979 214 302 381 2302
Fragments arranged by size:
2302 1979 593 582 555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 225 909 1874 2975 5200 5254 6738 6903
Size: 175 684 965 1101 2225 54 1484 165
Cuts at: 6903 50
Size: 55
Fragments arranged by size:
2225 1484 1101 965 684 175 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1598 3786 4820 1598
Size: 2188 1034 3686
Fragments arranged by size:
3686 2188 1034
BsrFI r'CCGG_y
Cuts at: 1209 2204 4906 5087 5296 1209
Size: 995 2702 181 209 2821
Fragments arranged by size:
2821 2702 995 209 181
BsrGI T'GTAC_A
Cuts at: 1550 1550
Size: 6908
BssSI C'ACGA_G
Cuts at: 995 2029 5179 5537 995
Size: 1034 3150 358 2366
Fragments arranged by size:
3150 2366 1034 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2214 4285 2214
Size: 2071 4837
BstBI TT'CG_AA
Cuts at: 5269 5269
Size: 6908
BstEII G'GTnAC_C
Cuts at: 1105 1105
Size: 6908
BstYI r'GATC_y
Cuts at: 1 888 1073 1868 2388 2549 3281 3371 3933
Size: 887 185 795 520 161 732 90 562
Cuts at: 3933 4400 4423 4758 5004 6016 6102 6114 6460
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6460 1
Size: 449
Fragments arranged by size:
1012 887 795 732 562 520 467 449
346 335 246 185 161 90 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 2640 2699 2640
Size: 59 6849
BtgI C'CryG_G
Cuts at: 116 1221 1406 1880 2041 2064 2481 116
Size: 1105 185 474 161 23 417 4543
Fragments arranged by size:
4543 1105 474 417 185 161 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 520 1508 2477 2868 4858 5039 5202 520
Size: 988 969 391 1990 181 163 2226
Fragments arranged by size:
2226 1990 988 969 391 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1329 1860 4151 648
Size: 681 531 2291 3405
Fragments arranged by size:
3405 2291 681 531
ClaI AT'CG_AT
Cuts at: 754 754
Size: 6908
DraI TTT'AAA
Cuts at: 633 3907 633
Size: 3274 3634
DraIII CAC_nnn'GTG
Cuts at: 2214 2214
Size: 6908
DrdI GACnn_nn'nnGTC
Cuts at: 2908 4614 5472 2908
Size: 1706 858 4344
Fragments arranged by size:
4344 1706 858
EaeI y'GGCC_r
Cuts at: 1358 2202 3667 4493 4667 5058 5085 6430 1358
Size: 844 1465 826 174 391 27 1345 1836
Fragments arranged by size:
1836 1465 1345 844 826 391 174 27
EagI C'GGCC_G
Cuts at: 2202 4493 2202
Size: 2291 4617
EarI CTCTTCn'nnn_
Cuts at: 145 724 1717 3093 3680 3868 4931 5141 145
Size: 579 993 1376 587 188 1063 210 1912
Fragments arranged by size:
1912 1376 1063 993 587 579 210 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 316 2217 3097 5116 5426 5572 6718 316
Size: 1901 880 2019 310 146 1146 506
Fragments arranged by size:
2019 1901 1146 880 506 310 146
EcoICRI GAG'CTC
Cuts at: 696 2253 2322 2793 2895 3920 696
Size: 1557 69 471 102 1025 3684
Fragments arranged by size:
3684 1557 1025 471 102 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 288 1605 1911 2142 2347 2755 3052 3628
Size: 95 1317 306 231 205 408 297 576
Cuts at: 3628 3697 3817 4733 5165 5912 6180 193
Size: 69 120 916 432 747 268 921
Fragments arranged by size:
1317 921 916 747 576 432 408 306
297 268 231 205 120 95 69
EcoO109I rG'GnC_Cy
Cuts at: 1483 1892 2108 2222 3048 4047 1483
Size: 409 216 114 826 999 4344
Fragments arranged by size:
4344 999 826 409 216 114
EcoRI G'AATT_C
Cuts at: 766 3912 766
Size: 3146 3762
EcoRV GAT'ATC
Cuts at: 306 306
Size: 6908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 1635 2961 3709 4927 630
Size: 1005 1326 748 1218 2611
Fragments arranged by size:
2611 1326 1218 1005 748
FspI TGC'GCA
Cuts at: 1156 3778 4689 1156
Size: 2622 911 3375
Fragments arranged by size:
3375 2622 911
HaeII r_GCGC'y
Cuts at: 2334 4342 4590 5612 6591 2334
Size: 2008 248 1022 979 2651
Fragments arranged by size:
2651 2008 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2341 2373 4750 4780 4782 4812 2341
Size: 32 2377 30 2 30 4437
Fragments arranged by size:
4437 2377 32 30 30 2
HincII GTy'rAC
Cuts at: 468 1397 1506 3024 3084 3947 468
Size: 929 109 1518 60 863 3429
Fragments arranged by size:
3429 1518 929 863 109 60
HindIII A'AGCT_T
Cuts at: 1136 1136
Size: 6908
HpaI GTT'AAC
Cuts at: 468 468
Size: 6908
KasI G'GCGC_C
Cuts at: 4338 4586 4338
Size: 248 6660
KpnI G_GTAC'C
Cuts at: 3928 3928
Size: 6908
MluI A'CGCG_T
Cuts at: 6341 6341
Size: 6908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 3475 5579 5763 2594
Size: 881 2104 184 3739
Fragments arranged by size:
3739 2104 881 184
MscI TGG'CCA
Cuts at: 1360 4669 1360
Size: 3309 3599
MslI CAynn'nnrTG
Cuts at: 689 2086 3172 4897 5024 689
Size: 1397 1086 1725 127 2573
Fragments arranged by size:
2573 1725 1397 1086 127
MspA1I CmG'CkG
Cuts at: 445 1113 1148 1428 1446 2066 3714 3837 4019
Size: 668 35 280 18 620 1648 123 182
Cuts at: 4019 4333 4693 5706 5951 445
Size: 314 360 1013 245 1402
Fragments arranged by size:
1648 1402 1013 668 620 360 314 280
245 182 123 35 18
NaeI GCC'GGC
Cuts at: 2206 5089 2206
Size: 2883 4025
NarI GG'CG_CC
Cuts at: 4339 4587 4339
Size: 248 6660
NcoI C'CATG_G
Cuts at: 1221 1880 1221
Size: 659 6249
NgoMIV G'CCGG_C
Cuts at: 2204 5087 2204
Size: 2883 4025
NspI r_CATG'y
Cuts at: 1041 1557 2085 3171 3961 4461 5368 1041
Size: 516 528 1086 790 500 907 2581
Fragments arranged by size:
2581 1086 907 790 528 516 500
PasI CC'CwG_GG
Cuts at: 331 331
Size: 6908
PciI A'CATG_T
Cuts at: 1553 5364 1553
Size: 3811 3097
PflMI CCAn_nnn'nTGG
Cuts at: 2042 2042
Size: 6908
PfoI T'CCnGG_A
Cuts at: 2324 2732 3674 2324
Size: 408 942 5558
Fragments arranged by size:
5558 942 408
PmeI GTTT'AAAC
Cuts at: 3907 3907
Size: 6908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1687 1719 6047 6079 1687
Size: 32 4328 32 2516
Fragments arranged by size:
4328 2516 32 32
PpuMI rG'GwC_Cy
Cuts at: 1892 2222 1892
Size: 330 6578
PshAI GACnn'nnGTC
Cuts at: 3058 3058
Size: 6908
PspOMI G'GGCC_C
Cuts at: 1195 1482 1195
Size: 287 6621
PspXI vC'TCGA_Gb
Cuts at: 1296 1296
Size: 6908
PstI C_TGCA'G
Cuts at: 1336 1751 2598 3224 3955 4640 1336
Size: 415 847 626 731 685 3604
Fragments arranged by size:
3604 847 731 685 626 415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6908
PvuII CAG'CTG
Cuts at: 445 1113 1428 1446 3714 3837 4333 4693 445
Size: 668 315 18 2268 123 496 360 2660
Fragments arranged by size:
2660 2268 668 496 360 315 123 18
RsrII CG'GwC_CG
Cuts at: 5103 5103
Size: 6908
SacI G_AGCT'C
Cuts at: 698 2255 2324 2795 2897 3922 698
Size: 1557 69 471 102 1025 3684
Fragments arranged by size:
3684 1557 1025 471 102 69
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 6908
SalI G'TCGA_C
Cuts at: 3022 3945 3022
Size: 923 5985
SanDI GG'GwC_CC
Cuts at: 1892 1892
Size: 6908
SapI GCTCTTCn'nnn_
Cuts at: 145 4931 5141 145
Size: 4786 210 1912
Fragments arranged by size:
4786 1912 210
SbfI CC_TGCA'GG
Cuts at: 3955 3955
Size: 6908
ScaI AGT'ACT
Cuts at: 2560 2863 6425 2560
Size: 303 3562 3043
Fragments arranged by size:
3562 3043 303
SexAI A'CCwGG_T
Cuts at: 2903 2903
Size: 6908
SfcI C'TryA_G
Cuts at: 33 1332 1676 1747 2594 3220 3951 4636 5629
Size: 1299 344 71 847 626 731 685 993
Cuts at: 5629 5820 33
Size: 191 1121
Fragments arranged by size:
1299 1121 993 847 731 685 626 344
191 71
SfoI GGC'GCC
Cuts at: 4340 4588 4340
Size: 248 6660
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
SmaI CCC'GGG
Cuts at: 2101 2517 3930 2101
Size: 416 1413 5079
Fragments arranged by size:
5079 1413 416
SmlI C'TyrA_G
Cuts at: 1296 4063 4978 5470 5732 1296
Size: 2767 915 492 262 2472
Fragments arranged by size:
2767 2472 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6908
SphI G_CATG'C
Cuts at: 3961 4461 3961
Size: 500 6408
SspI AAT'ATT
Cuts at: 2663 2663
Size: 6908
StuI AGG'CCT
Cuts at: 1366 1366
Size: 6908
StyI C'CwwG_G
Cuts at: 1221 1361 1880 2507 2843 2936 4042 4396 1221
Size: 140 519 627 336 93 1106 354 3733
Fragments arranged by size:
3733 1106 627 519 354 336 140 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4491 4624 5083 5292 4491
Size: 133 459 209 6107
Fragments arranged by size:
6107 459 209 133
TatI w'GTAC_w
Cuts at: 1550 2558 2861 3035 3164 6423 1550
Size: 1008 303 174 129 3259 2035
Fragments arranged by size:
3259 2035 1008 303 174 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2488 2520 3131 3163 6425 6457 2488
Size: 32 611 32 3262 32 2939
Fragments arranged by size:
3262 2939 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4705 4705
Size: 6908
XbaI T'CTAG_A
Cuts at: 3939 3939
Size: 6908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2781 4403 2781
Size: 1622 5286
XhoI C'TCGA_G
Cuts at: 1296 1296
Size: 6908
XmaI C'CCGG_G
Cuts at: 2099 2515 3928 2099
Size: 416 1413 5079
Fragments arranged by size:
5079 1413 416
XmnI GAAnn'nnTTC
Cuts at: 1702 1702
Size: 6908
ZraI GAC'GTC
Cuts at: 1260 2370 1260
Size: 1110 5798
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BssHII BstXI BstZ17I CspCI EcoNI
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PsrI SfiI SgrAI SnaBI SrfI
SwaI