Restriction map for pF1KB3248
##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwI          160  GATCC                GGATC(4/5)
AvaI          539  CTCGAG               C^[CT]CG[GA]G
AvaII         556  GGACC                G^G[AT]CC
BalI          336  TGGCCA               TGG^CCA
BfaI          224  CTAG                 C^TAG
BpmI          453  CTGGAG               CTGGAG(16/14)
BseRI          55  CTCCTC               GAGGAG(10/8)
BsoBI         539  CTCGAG               C^[CT]CG[GA]G
BspHI         117  TCATGA               T^CATGA
BstEII          6  GGTGACC              G^GT[ACGT]ACC
BstF5I         75  CATCC                GGATG(2/0)
BstPI           6  GGTGACC              G^GT[ACGT]ACC
BstYI         159  AGATCC               [GA]^GATC[CT]
Cac8I          21  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr13I        556  GGACC                G^G[ACGT]CC
DpnII         160  GATC                 ^GATC
DraII         555  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          336  TGGCCA               [CT]^GGCC[GA]
EcoO109I      555  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I         6  GGTGACC              G^GT[ACGT]ACC
EcoRI         121  GAATTC               G^AATTC
FokI           75  CATCC                GGATG(9/13)
HaeIII        337  GGCC                 GG^CC
HgaI          398  GACGC                GACGC(5/10)
HphI            6  GGTGA                GGTGA(8/7)
MaeI          224  CTAG                 C^TAG
MboI          160  GATC                 ^GATC
MflI          159  AGATCC               [GA]^GATC[CT]
MluNI         336  TGGCCA               TGG^CCA
MscI          336  TGGCCA               TGG^CCA
NdeII         160  GATC                 ^GATC
PaeR7I        539  CTCGAG               C^TCGAG
PpuMI         555  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          117  TCATGA               T^CATGA
Sau3AI        160  GATC                 ^GATC
Sau96I        556  GGACC                G^G[ACGT]CC
SmlI          539  CTCGAG               C^T[CT][GA]AG
XhoI          539  CTCGAG               C^TCGAG
XhoII         159  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI          221  GTAC                 GT^AC
              586  GTAC                
BsaJI         451  CCCTGG               C^C[ACGT][ACGT]GG
              559  CCCTGG              
BsmFI          90  GGGAC                GGGAC(10/14)
              555  GGGAC               
BssKI         452  CCTGG                ^CC[ACGT]GG
              560  CCTGG               
BstNI         452  CCTGG                CC^[AT]GG
              560  CCTGG               
DdeI          428  CTAAG                C^T[ACGT]AG
              611  CTCAG               
EcoRII        452  CCTGG                ^CC[AT]GG
              560  CCTGG               
MaeIII          7  GTGAC                ^GT[ACGT]AC
              602  GTGAC               
MvaI          452  CCTGG                CC^[AT]GG
              560  CCTGG               
MwoI           21  GCCAGCCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               30  GCTGCTGCAGC         
NspI           39  GCATGT               [GA]CATG^[CT]
              308  GCATGT              
PstI           34  CTGCAG               CTGCA^G
              197  CTGCAG              
RsaI          221  GTAC                 GT^AC
              586  GTAC                
ScrFI         452  CCTGG                CC^[ACGT]GG
              560  CCTGG               
Tsp45I          7  GTGAC                ^GT[GC]AC
              602  GTGAC               
TspRI         167  CACTG                CA[GC]TG[ACGT][ACGT]^
              613  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        Fnu4HI      
ItaI        MboII       MnlI        NlaIII      NlaIV       
SfaNI       SfcI        TaqI        TseI        Tsp509I     
TthHB8I     
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