Restriction map for pF1KB3230
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BalI        BbeI        
BbrPI       BcgI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FseI        
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        NarI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1774  GACGTC               GACGT^C
AccIII        495  TCCGGA               T^CCGGA
AflIII        633  ACATGT               A^C[GA][CT]GT
Alw44I       1234  GTGCAC               G^TGCAC
AlwNI          81  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          277  GGGCCC               GGGCC^C
ApaLI        1234  GTGCAC               G^TGCAC
AvaI          986  CCCGAG               C^[CT]CG[GA]G
BanI         1252  GGCACC               G^G[CT][GA]CC
BbvCI         192  CCTCAGC              CCTCAGC(-5/-2)
BciVI         415  GTATCC               GTATCC(6/5)
BclI          855  TGATCA               T^GATCA
BcnI          510  CCGGG                CC^[GC]GG
BlpI          772  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      772  GCTCAGC              GC^T[ACGT]AGC
BseAI         495  TCCGGA               T^CCGGA
BseRI         676  GAGGAG               GAGGAG(10/8)
BsmFI        1383  GTCCC                GGGAC(10/14)
BsoBI         986  CCCGAG               C^[CT]CG[GA]G
BspEI         495  TCCGGA               T^CCGGA
BspLU11I      633  ACATGT               A^CATGT
CelII         772  GCTCAGC              GC^T[ACGT]AGC
DraI         1342  TTTAAA               TTT^AAA
DsaI          905  CCATGG               C^C[GA][CT]GG
EaeI         1500  TGGCCG               [CT]^GGCC[GA]
EcoT14I       905  CCATGG               C^C[AT][AT]GG
EcoT22I       647  ATGCAT               ATGCA^T
FbaI          855  TGATCA               T^GATCA
HaeII        1611  GGCGCT               [GA]GCGC^[CT]
HincII        629  GTCAAC               GT[CT]^[GA]AC
HindII        629  GTCAAC               GT[CT]^[GA]AC
MroI          495  TCCGGA               T^CCGGA
NciI          510  CCGGG                CC^[GC]GG
NcoI          905  CCATGG               C^CATGG
NdeI         1070  CATATG               CA^TATG
NsiI          647  ATGCAT               ATGCA^T
PshAI         396  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1243  CTGCAG               CTGCA^G
PvuII        1879  CAGCTG               CAG^CTG
SapI         1051  GAAGAGC              GCTCTTC(1/4)
SmlI          217  CTCAAG               C^T[CT][GA]AG
StyI          905  CCATGG               C^C[AT][AT]GG
XbaI          207  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         863  CGCG                 CG^CG
             1693  CGCG                
AcyI          319  GACGCC               G[GA]^CG[CT]C
             1774  GACGTC              
AviII          73  TGCGCA               TGC^GCA
             1785  TGCGCA              
BamHI         372  GGATCC               G^GATCC
             1350  GGATCC              
BbsI          393  GAAGAC               GAAGAC(2/6)
             1323  GAAGAC              
BpuAI         393  GAAGAC               GAAGAC(2/6)
             1323  GAAGAC              
BsaHI         319  GACGCC               G[GA]^CG[CT]C
             1774  GACGTC              
BsaWI         495  TCCGGA               [AT]^CCGG[AT]
             1150  ACCGGA              
BsgI         1059  CTGCAC               GTGCAG(16/14)
             1869  CTGCAC              
BspMI          25  ACCTGC               ACCTGC(4/8)
              172  ACCTGC              
BsrFI         176  GCCGGC               [GA]^CCGG[CT]
             1502  GCCGGC              
BstAPI        791  GCAAACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1236  GCACGGTCTGC         
BstUI         863  CGCG                 CG^CG
             1693  CGCG                
BstYI         372  GGATCC               [GA]^GATC[CT]
             1350  GGATCC              
Cfr10I        176  GCCGGC               [GA]^CCGG[CT]
             1502  GCCGGC              
DraII          53  AGGACCC              [GA]G^G[ACGT]CC[CT]
              347  AGGTCCT             
DraIII        119  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1809  CACCGTGTG           
EcoO109I       53  AGGACCC              [GA]G^G[ACGT]CC[CT]
              347  AGGTCCT             
FspI           73  TGCGCA               TGC^GCA
             1785  TGCGCA              
HgaI          319  GACGC                GACGC(5/10)
              385  GACGC               
Hin1I         319  GACGCC               G[GA]^CG[CT]C
             1774  GACGTC              
MflI          372  GGATCC               [GA]^GATC[CT]
             1350  GGATCC              
MseI          578  TTAA                 T^TAA
             1343  TTAA                
MspA1I       1620  CCGCTG               C[AC]G^C[GT]G
             1879  CAGCTG              
MvnI          863  CGCG                 CG^CG
             1693  CGCG                
NaeI          176  GCCGGC               GCC^GGC
             1502  GCCGGC              
NgoMIV        176  GCCGGC               G^CCGGC
             1502  GCCGGC              
NspI          180  GCATGT               [GA]CATG^[CT]
              633  ACATGT              
PpuMI          53  AGGACCC              [GA]G^G[AT]CC[CT]
              347  AGGTCCT             
Tru9I         578  TTAA                 T^TAA
             1343  TTAA                
XcmI          131  CCAACGAGTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1458  CCAGGGGAGCTATGG     
XhoII         372  GGATCC               [GA]^GATC[CT]
             1350  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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