Restriction map for pF1KB3226
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BbrPI       BbvCI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EarI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NcoI        NdeI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AflIII       1337  ACATGT               A^C[GA][CT]GT
Asp700I      1572  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1759  GGATCC               G^GATCC
BlpI         1816  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1816  GCTGAGC              GC^T[ACGT]AGC
BsiEI        1028  CGGCCG               CG[GA][CT]^CG
BsmFI         943  GTCCC                GGGAC(10/14)
BspLU11I     1337  ACATGT               A^CATGT
BsrBI         532  CCGCTC               CCGCTC(-3/-3)
BstBI        1187  TTCGAA               TT^CGAA
BstXI        1235  CCATCTGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1816  GCTGAGC              GC^T[ACGT]AGC
DraIII        126  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1028  CGGCCG               C^GGCCG
EclXI        1028  CGGCCG               C^GGCCG
Eco52I       1028  CGGCCG               C^GGCCG
EcoNI         183  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1853  CCAAGG               C^C[AT][AT]GG
HincII        622  GTCAAC               GT[CT]^[GA]AC
HindII        622  GTCAAC               GT[CT]^[GA]AC
MseI         1157  TTAA                 T^TAA
NheI         1792  GCTAGC               G^CTAGC
NspV         1187  TTCGAA               TT^CGAA
PpuMI         941  GGGTCCC              [GA]G^G[AT]CC[CT]
SfuI         1187  TTCGAA               TT^CGAA
SgrAI         490  CGCCGGCG             C[GA]^CCGG[CT]G
StyI         1853  CCAAGG               C^C[AT][AT]GG
Tru9I        1157  TTAA                 T^TAA
XcmI          644  CCAACGCCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1572  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AhdI          223  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              292  GACCCGTCGTC         
AlwNI         102  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              610  CAGTGCCTG           
AspEI         223  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              292  GACCCGTCGTC         
BalI         1332  TGGCCA               TGG^CCA
             1361  TGGCCA              
BbsI         1431  GAAGAC               GAAGAC(2/6)
             1670  GAAGAC              
BcgI          237  GCACCAGTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              311  GCAAGGGCATCG        
BciVI        1013  GGATAC               GTATCC(6/5)
             1837  GTATCC              
BpmI          722  CTCCAG               CTGGAG(16/14)
              713  CTGGAG              
BpuAI        1431  GAAGAC               GAAGAC(2/6)
             1670  GAAGAC              
BsgI         1072  CTGCAC               GTGCAG(16/14)
             1230  CTGCAC              
BssSI        1309  CTCGTG               CACGAG(-5/-1)
             1357  CTCGTG              
BstYI        1736  AGATCC               [GA]^GATC[CT]
             1759  GGATCC              
DpnII        1737  GATC                 ^GATC
             1760  GATC                
Eam1105I      223  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              292  GACCCGTCGTC         
MaeIII          3  GTCAC                ^GT[ACGT]AC
             1255  GTCAC               
MboI         1737  GATC                 ^GATC
             1760  GATC                
MflI         1736  AGATCC               [GA]^GATC[CT]
             1759  GGATCC              
MluNI        1332  TGGCCA               TGG^CCA
             1361  TGGCCA              
MscI         1332  TGGCCA               TGG^CCA
             1361  TGGCCA              
NaeI          491  GCCGGC               GCC^GGC
              790  GCCGGC              
NdeII        1737  GATC                 ^GATC
             1760  GATC                
NgoMIV        491  GCCGGC               G^CCGGC
              790  GCCGGC              
PleI         1673  GACTC                GAGTC(4/5)
             1711  GAGTC               
SacI         1306  GAGCTC               GAGCT^C
             1819  GAGCTC              
Sau3AI       1737  GATC                 ^GATC
             1760  GATC                
SfiI         1315  GGCCGCCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1324  GGCCGCTGTGGCC       
TfiI         1170  GAATC                G^A[AT]TC
             1592  GATTC               
Tsp45I          3  GTCAC                ^GT[GC]AC
             1255  GTCAC               
XhoII        1736  AGATCC               [GA]^GATC[CT]
             1759  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BfaI        BglI        BmyI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssHII      BssKI       
BstAPI      BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeI        
MaeII       MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NlaIII      NlaIV       NspI        PstI        
RsaI        Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        TaqI        TseI        TspRI       
TthHB8I     XmaCI       XmaI        
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