Restriction map for pF1KB3226
 (Circular) (Six-base) MAPSORT of: pF1KB3226.seq  Check: 443  from: 1  to: 4973



pF1KB3226  4973 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    288    1463    1525     288 
   Size:       1175      62    3736

  Fragments arranged by size: 

                3736    1175      62
 
AccI GT'mk_AC

Cuts at:   2011    2011 
   Size:       4973
 
Acc65I G'GTAC_C

Cuts at:   1989    1989 
   Size:       4973
 
AclI AA'CG_TT

Cuts at:   4813    4813 
   Size:       4973
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    283    2798    3230    3583    4245     283 
   Size:       2515     432     353     662    1011

  Fragments arranged by size: 

                2515    1011     662     432     353
 
AflIII A'CryG_T

Cuts at:   1445    2325    4125    4406    1445 
   Size:        880    1800     281    2012

  Fragments arranged by size: 

                2012    1800     880     281
 
AhdI GACnn_n'nnGTC

Cuts at:    336     405     336 
   Size:         69    4904
 
AleI CACnn'nnGTG

Cuts at:   1018    1831    1018 
   Size:        813    4160
 
AlwNI CAG_nnn'CTG

Cuts at:    215     723    2278    3716     215 
   Size:        508    1555    1438    1472

  Fragments arranged by size: 

                1555    1472    1438     508
 
ApaI G_GGCC'C

Cuts at:   1004    1167    1660    1004 
   Size:        163     493    4317

  Fragments arranged by size: 

                4317     493     163
 
ApaLI G'TGCA_C

Cuts at:   3811    3811 
   Size:       4973
 
ApoI r'AATT_y

Cuts at:   1977    4326    4582    1977 
   Size:       2349     256    2368

  Fragments arranged by size: 

                2368    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4973
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4973
 
AvaI C'yCGr_G

Cuts at:    176     693     925     945     972     997    1231    1282    1675 
   Size:        517     232      20      27      25     234      51     393

Cuts at:   1675    1993     176 
   Size:        318    3156

  Fragments arranged by size: 

                3156     517     393     318     234     232      51      27
                  25      20
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1114    1147    1114 
   Size:         33    4940
 
BamHI G'GATC_C

Cuts at:   1867    1998    1867 
   Size:        131    4842
 
BanI G'GyrC_C

Cuts at:    367     411     602     685     783     871     930     949    1989 
   Size:         44     191      83      98      88      59      19    1040

Cuts at:   1989    2403    2651    2686     367 
   Size:        414     248      35    2654

  Fragments arranged by size: 

                2654    1040     414     248     191      98      88      83
                  59      44      35      19
 
BanII G_rGCy'C

Cuts at:    655     914     946    1004    1167    1418    1660    1931    1987 
   Size:        259      32      58     163     251     242     271      56

Cuts at:   1987    3017     655 
   Size:       1030    2611

  Fragments arranged by size: 

                2611    1030     271     259     251     242     163      58
                  56      32
 
BbeI G_GCGC'C

Cuts at:    415     606     689     787     875     934     953    2407    2655 
   Size:        191      83      98      88      59      19    1454     248

Cuts at:   2655     415 
   Size:       2733

  Fragments arranged by size: 

                2733    1454     248     191      98      88      83      59
                  19
 
BbsI GAAGACnn'nnnn_

Cuts at:   1546    1785    1546 
   Size:        239    4734
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    334     368     408     442     334 
   Size:         34      40      34    4865

  Fragments arranged by size: 

                4865      40      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1115    1956    2871    3927    4973    1115 
   Size:        841     915    1056    1046    1115

  Fragments arranged by size: 

                1115    1056    1046     915     841
 
BclI T'GATC_A

Cuts at:   2493    2493 
   Size:       4973
 
BglI GCCn_nnn'nGGC

Cuts at:    384     763    1430    1439     384 
   Size:        379     667       9    3918

  Fragments arranged by size: 

                3918     667     379       9
 
BglII A'GATC_T

Cuts at:   2488    4525    2488 
   Size:       2037    2936
 
BlpI GC'TnA_GC

Cuts at:   1925    2085    1925 
   Size:        160    4813
 
Bme1580I G_kGCm'C

Cuts at:    320    1004    1167    1232    1513    1660    2598    2691    3815 
   Size:        684     163      65     281     147     938      93    1124

Cuts at:   3815     320 
   Size:       1478

  Fragments arranged by size: 

                1478    1124     938     684     281     163     147      93
                  65
 
BmgBI CAC'GTC

Cuts at:   1209    1802    1209 
   Size:        593    4380
 
BmrI ACTGGGnnnn_n'

Cuts at:   1094    2185    2266    2601    4745    1094 
   Size:       1091      81     335    2144    1322

  Fragments arranged by size: 

                2144    1322    1091     335      81
 
BmtI G_CTAG'C

Cuts at:   1904    1904 
   Size:       4973
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3340    3340 
   Size:       4973
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    815     842     815 
   Size:         27    4946
 
Bpu10I CC'TnA_GC

Cuts at:   1412    1661    1412 
   Size:        249    4724
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2149    3028    3742    4040    2149 
   Size:        879     714     298    3082

  Fragments arranged by size: 

                3082     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4677    4677 
   Size:       4973
 
BsaAI yAC'GTr

Cuts at:   2326    2326 
   Size:       4973
 
BsaBI GATnn'nnATC

Cuts at:      6    2487    4524       6 
   Size:       2481    2037     455

  Fragments arranged by size: 

                2481    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    305     412     603     686     784     872     931     950    2404 
   Size:        107     191      83      98      88      59      19    1454

Cuts at:   2404    2652    3354    4850     305 
   Size:        248     702    1496     428

  Fragments arranged by size: 

                1496    1454     702     428     248     191     107      98
                  88      83      59      19
 
BsaWI w'CCGG_w

Cuts at:   2162    2683    3361    3772    3919    4193    2162 
   Size:        521     678     411     147     274    2942

  Fragments arranged by size: 

                2942     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    308     853    1004    2398    3156    3821    4229     308 
   Size:        545     151    1394     758     665     408    1052

  Fragments arranged by size: 

                1394    1052     758     665     545     408     151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1165    1323    1165 
   Size:        158    4815
 
BsiEI CG_ry'CG

Cuts at:    104    1139    2515    2561    3791     104 
   Size:       1035    1376      46    1230    1286

  Fragments arranged by size: 

                1376    1286    1230    1035      46
 
BsiHKAI G_wGCw'C

Cuts at:    209    1418    1667    1931    1987    2765    2955    3815     209 
   Size:       1209     249     264      56     778     190     860    1367

  Fragments arranged by size: 

                1367    1209     860     778     264     249     190      56
            
 
Bsp1286I G_dGCh'C

Cuts at:    209     320     655     914     946    1004    1167    1232    1418 
   Size:        111     335     259      32      58     163      65     186

Cuts at:   1418    1513    1660    1667    1931    1987    2598    2691    2765 
   Size:         95     147       7     264      56     611      93      74

Cuts at:   2765    2955    3017    3815     209 
   Size:        190      62     798    1367

  Fragments arranged by size: 

                1367     798     611     335     264     259     190     186
                 163     147     111      95      93      74      65      62
                  58      56      32       7
 
BspHI T'CATG_A

Cuts at:   3405    3405 
   Size:       4973
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    288     341    1463    1525    2023    2237    2539    2920     288 
   Size:         53    1122      62     498     214     302     381    2341

  Fragments arranged by size: 

                2341    1122     498     381     302     214      62      53
            
 
BsrBI CCG'CTC

Cuts at:     50     642    3265    3319    4803    4968      50 
   Size:        592    2623      54    1484     165      55

  Fragments arranged by size: 

                2623    1484     592     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2885    2885 
   Size:       4973
 
BsrFI r'CCGG_y

Cuts at:    599     898     992    1089    2971    3152     599 
   Size:        299      94      97    1882     181    2420

  Fragments arranged by size: 

                2420    1882     299     181      97      94
 
BssHII G'CGCG_C

Cuts at:    741     836     964    1025    1027    1029     741 
   Size:         95     128      61       2       2    4685

  Fragments arranged by size: 

                4685     128      95      61       2       2
 
BssSI C'ACGA_G

Cuts at:   1417    1465    3244    3952    1417 
   Size:         48    1779     708    2438

  Fragments arranged by size: 

                2438    1779     708      48
 
BstAPI GCAn_nnn'nTGC

Cuts at:    342     723    1346    2350     342 
   Size:        381     623    1004    2965

  Fragments arranged by size: 

                2965    1004     623     381
 
BstBI TT'CG_AA

Cuts at:   1296    3334    1296 
   Size:       2038    2935
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1350    1350 
   Size:       4973
 
BstYI r'GATC_y

Cuts at:      1    1844    1867    1998    2465    2488    2823    3069    3375 
   Size:       1843      23     131     467      23     335     246     306

Cuts at:   3375    3387    3473    4525       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1843    1052     467     449     335     306     246     131
                  86      23      23      12
 
BtgI C'CryG_G

Cuts at:    123     284     520     647     680     937    1811    1829    1884 
   Size:        161     236     127      33     257     874      18      55

Cuts at:   1884     123 
   Size:       3212

  Fragments arranged by size: 

                3212     874     257     236     161     127      55      33
                  18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    521    2923    3104    3267     521 
   Size:       2402     181     163    2227

  Fragments arranged by size: 

                2402    2227     181     163
 
BtsI GCAGTG_nn'

Cuts at:    724    1513    1710    2216     724 
   Size:        789     197     506    3481

  Fragments arranged by size: 

                3481     789     506     197
 
DraI TTT'AAA

Cuts at:   1972    1972 
   Size:       4973
 
DraIII CAC_nnn'GTG

Cuts at:    239     239 
   Size:       4973
 
DrdI GACnn_nn'nnGTC

Cuts at:   2679    4023    2679 
   Size:       1344    3629
 
EaeI y'GGCC_r

Cuts at:    115     230    1136    1440    1469    2558    2732    3123    3150 
   Size:        115     906     304      29    1089     174     391      27

Cuts at:   3150    4495     115 
   Size:       1345     593

  Fragments arranged by size: 

                1345    1089     906     593     391     304     174     115
                  29      27
 
EagI C'GGCC_G

Cuts at:   1136    2558    1136 
   Size:       1422    3551
 
EarI CTCTTCn'nnn_

Cuts at:   2996    3206    2996 
   Size:        210    4763
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    974    3181    3923    4069    4783     974 
   Size:       2207     742     146     714    1164

  Fragments arranged by size: 

                2207    1164     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1416    1929    1985    1416 
   Size:        513      56    4404

  Fragments arranged by size: 

                4404     513      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     815     842    2798    3230    3583    4245     283 
   Size:        532      27    1956     432     353     662    1011

  Fragments arranged by size: 

                1956    1011     662     532     432     353      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:    295     295 
   Size:       4973
 
EcoO109I rG'GnC_Cy

Cuts at:    826    1001    1050    1164    1656    1657    2112     826 
   Size:        175      49     114     492       1     455    3687

  Fragments arranged by size: 

                3687     492     455     175     114      49       1
 
EcoRI G'AATT_C

Cuts at:   1977    1977 
   Size:       4973
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2992    2992 
   Size:       4973
 
FspI TGC'GCA

Cuts at:   2754    2754 
   Size:       4973
 
HaeII r_GCGC'y

Cuts at:    415     606     689     777     787     860     875     892     934 
   Size:        191      83      88      10      73      15      17      42

Cuts at:    934     953    2407    2655    3885    4656     415 
   Size:         19    1454     248    1230     771     732

  Fragments arranged by size: 

                1454    1230     771     732     248     191      88      83
                  73      42      19      17      15      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    322     354     391     423     520     552    1263    1295    1852 
   Size:         32      37      32      97      32     711      32     557

Cuts at:   1852    1859    1884    1891    2815    2845    2847    2877     322 
   Size:          7      25       7     924      30       2      30    2418

  Fragments arranged by size: 

                2418     924     711     557      97      37      32      32
                  32      32      30      30      25       7       7       2
            
 
HincII GTy'rAC

Cuts at:    732    2012     732 
   Size:       1280    3693
 
KasI G'GCGC_C

Cuts at:    411     602     685     783     871     930     949    2403    2651 
   Size:        191      83      98      88      59      19    1454     248

Cuts at:   2651     411 
   Size:       2733

  Fragments arranged by size: 

                2733    1454     248     191      98      88      83      59
                  19
 
KpnI G_GTAC'C

Cuts at:   1993    1993 
   Size:       4973
 
MluI A'CGCG_T

Cuts at:   4406    4406 
   Size:       4973
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    776    1802    1829    1895    3732    3916     776 
   Size:       1026      27      66    1837     184    1833

  Fragments arranged by size: 

                1837    1833    1026     184      66      27
 
MscI TGG'CCA

Cuts at:   1442    1471    2734    1442 
   Size:         29    1263    3681

  Fragments arranged by size: 

                3681    1263      29
 
MslI CAynn'nnrTG

Cuts at:   1018    1348    1831    2962    3089    1018 
   Size:        330     483    1131     127    2902

  Fragments arranged by size: 

                2902    1131     483     330     127
 
MspA1I CmG'CkG

Cuts at:    215     801    1436    2084    2398    2758    3542    3787     215 
   Size:        586     635     648     314     360     784     245    1401

  Fragments arranged by size: 

                1401     784     648     635     586     360     314     245
            
 
NaeI GCC'GGC

Cuts at:    601     900    3154     601 
   Size:        299    2254    2420

  Fragments arranged by size: 

                2420    2254     299
 
NarI GG'CG_CC

Cuts at:    412     603     686     784     872     931     950    2404    2652 
   Size:        191      83      98      88      59      19    1454     248

Cuts at:   2652     412 
   Size:       2733

  Fragments arranged by size: 

                2733    1454     248     191      98      88      83      59
                  19
 
NgoMIV G'CCGG_C

Cuts at:    599     898    3152     599 
   Size:        299    2254    2420

  Fragments arranged by size: 

                2420    2254     299
 
NheI G'CTAG_C

Cuts at:   1900    1900 
   Size:       4973
 
NspI r_CATG'y

Cuts at:    576    1308    1449    2026    2526    4129     576 
   Size:        732     141     577     500    1603    1420

  Fragments arranged by size: 

                1603    1420     732     577     500     141
 
PasI CC'CwG_GG

Cuts at:   1938    1938 
   Size:       4973
 
PciI A'CATG_T

Cuts at:   1445    4125    1445 
   Size:       2680    2293
 
PmeI GTTT'AAAC

Cuts at:   1972    1972 
   Size:       4973
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3419    3451    3419 
   Size:         32    4941
 
PpuMI rG'GwC_Cy

Cuts at:   1050    1050 
   Size:       4973
 
PspOMI G'GGCC_C

Cuts at:   1000    1163    1656    1000 
   Size:        163     493    4317

  Fragments arranged by size: 

                4317     493     163
 
PstI C_TGCA'G

Cuts at:    338    1508    1959    2020    2705     338 
   Size:       1170     451      61     685    2606

  Fragments arranged by size: 

                2606    1170     685     451      61
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4973
 
PvuII CAG'CTG

Cuts at:   2398    2758    2398 
   Size:        360    4613
 
RsrII CG'GwC_CG

Cuts at:   3168    3168 
   Size:       4973
 
SacI G_AGCT'C

Cuts at:   1418    1931    1987    1418 
   Size:        513      56    4404

  Fragments arranged by size: 

                4404     513      56
 
SalI G'TCGA_C

Cuts at:   2010    2010 
   Size:       4973
 
SanDI GG'GwC_CC

Cuts at:   1050    1050 
   Size:       4973
 
SapI GCTCTTCn'nnn_

Cuts at:   2996    3206    2996 
   Size:        210    4763
 
SbfI CC_TGCA'GG

Cuts at:   2020    2020 
   Size:       4973
 
ScaI AGT'ACT

Cuts at:   4490    4490 
   Size:       4973
 
SfcI C'TryA_G

Cuts at:     33     334    1504    1955    2016    2701    3669    3860      33 
   Size:        301    1170     451      61     685     968     191    1146

  Fragments arranged by size: 

                1170    1146     968     685     451     301     191      61
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1430    1439    1430 
   Size:          9    4964
 
SfoI GGC'GCC

Cuts at:    413     604     687     785     873     932     951    2405    2653 
   Size:        191      83      98      88      59      19    1454     248

Cuts at:   2653     413 
   Size:       2733

  Fragments arranged by size: 

                2733    1454     248     191      98      88      83      59
                  19
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4973
 
SgrAI Cr'CCGG_yG

Cuts at:    599     599 
   Size:       4973
 
SmaI CCC'GGG

Cuts at:    178     927     947     974     999    1233    1677    1995     178 
   Size:        749      20      27      25     234     444     318    3156

  Fragments arranged by size: 

                3156     749     444     318     234      27      25      20
            
 
SmlI C'TyrA_G

Cuts at:   2128    3043    3757    4019    2128 
   Size:        915     714     262    3082

  Fragments arranged by size: 

                3082     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4973
 
SphI G_CATG'C

Cuts at:   2026    2526    2026 
   Size:        500    4473
 
SrfI GCCC'GGGC

Cuts at:    947     999     947 
   Size:         52    4921
 
StyI C'CwwG_G

Cuts at:   1961    2107    2461    1961 
   Size:        146     354    4473

  Fragments arranged by size: 

                4473     354     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1873    2556    2689    3148    3357    1873 
   Size:        683     133     459     209    3489

  Fragments arranged by size: 

                3489     683     459     209     133
 
TatI w'GTAC_w

Cuts at:   1475    4488    1475 
   Size:       3013    1960
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4490    4522    4490 
   Size:         32    4941
 
Tth111I GACn'n_nGTC

Cuts at:   2770    2770 
   Size:       4973
 
XbaI T'CTAG_A

Cuts at:   2004    2004 
   Size:       4973
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    759    2468     759 
   Size:       1709    3264
 
XmaI C'CCGG_G

Cuts at:    176     925     945     972     997    1231    1675    1993     176 
   Size:        749      20      27      25     234     444     318    3156

  Fragments arranged by size: 

                3156     749     444     318     234      27      25      20
            
 
XmnI GAAnn'nnTTC

Cuts at:   1684    1684 
   Size:       4973
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NspI
     PasI     PciI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AlfI     AloI     AscI    AvrII
    BbvCI    BfrBI    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI
    BsrGI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI    SnaBI     SspI     StuI     SwaI     XhoI
     ZraI
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