(Circular) (Six-base) MAPSORT of: pF1KB3191.seq Check: 4801 from: 1 to: 4880 pF1KB3191 4880 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AatII G_ACGT'C Cuts at: 1832 1832 Size: 4880 AccI GT'mk_AC Cuts at: 1918 1918 Size: 4880 Acc65I G'GTAC_C Cuts at: 1896 1896 Size: 4880 AclI AA'CG_TT Cuts at: 4720 4720 Size: 4880 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 884 1246 1525 2705 3137 3490 4152 884 Size: 362 279 1180 432 353 662 1612 Fragments arranged by size: 1612 1180 662 432 362 353 279 AflIII A'CryG_T Cuts at: 1125 2232 4032 4313 1125 Size: 1107 1800 281 1692 Fragments arranged by size: 1800 1692 1107 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1642 1642 Size: 4880 AlwNI CAG_nnn'CTG Cuts at: 810 975 1317 2185 3623 810 Size: 165 342 868 1438 2067 Fragments arranged by size: 2067 1438 868 342 165 ApaI G_GGCC'C Cuts at: 1542 1754 1542 Size: 212 4668 ApaLI G'TGCA_C Cuts at: 364 3718 364 Size: 3354 1526 ApoI r'AATT_y Cuts at: 1884 4233 4489 1884 Size: 2349 256 2275 Fragments arranged by size: 2349 2275 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4880 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4880 AvaI C'yCGr_G Cuts at: 575 1900 575 Size: 1325 3555 BamHI G'GATC_C Cuts at: 1905 1905 Size: 4880 BanI G'GyrC_C Cuts at: 1896 2310 2558 2593 1896 Size: 414 248 35 4183 Fragments arranged by size: 4183 414 248 35 BanII G_rGCy'C Cuts at: 917 1542 1754 1894 2924 917 Size: 625 212 140 1030 2873 Fragments arranged by size: 2873 1030 625 212 140 BbeI G_GCGC'C Cuts at: 2314 2562 2314 Size: 248 4632 BbsI GAAGACnn'nnnn_ Cuts at: 872 872 Size: 4880 BbvCI CC'TCA_GC Cuts at: 1343 1343 Size: 4880 BciVI GTATCCnnnnn_n' Cuts at: 2778 3834 4880 2778 Size: 1056 1046 2778 Fragments arranged by size: 2778 1056 1046 BclI T'GATC_A Cuts at: 1070 2400 1070 Size: 1330 3550 BglI GCCn_nnn'nGGC Cuts at: 112 1849 112 Size: 1737 3143 BglII A'GATC_T Cuts at: 1082 2395 4432 1082 Size: 1313 2037 1530 Fragments arranged by size: 2037 1530 1313 BlpI GC'TnA_GC Cuts at: 1220 1992 1220 Size: 772 4108 Bme1580I G_kGCm'C Cuts at: 368 1240 1542 1754 2505 2598 3722 368 Size: 872 302 212 751 93 1124 1526 Fragments arranged by size: 1526 1124 872 751 302 212 93 BmrI ACTGGGnnnn_n' Cuts at: 1305 2092 2173 2508 4652 1305 Size: 787 81 335 2144 1533 Fragments arranged by size: 2144 1533 787 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 777 3247 777 Size: 2470 2410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 406 499 715 847 901 406 Size: 93 216 132 54 4385 Fragments arranged by size: 4385 216 132 93 54 Bpu10I CC'TnA_GC Cuts at: 1343 1343 Size: 4880 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2056 2935 3649 3947 2056 Size: 879 714 298 2989 Fragments arranged by size: 2989 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1165 1574 4584 1165 Size: 409 3010 1461 Fragments arranged by size: 3010 1461 409 BsaAI yAC'GTr Cuts at: 363 2233 363 Size: 1870 3010 BsaBI GATnn'nnATC Cuts at: 6 1294 2394 4431 6 Size: 1288 1100 2037 455 Fragments arranged by size: 2037 1288 1100 455 BsaHI Gr'CG_yC Cuts at: 1349 1829 2311 2559 3261 4757 1349 Size: 480 482 248 702 1496 1472 Fragments arranged by size: 1496 1472 702 482 480 248 BsaWI w'CCGG_w Cuts at: 617 1576 2069 2590 3268 3679 3826 4100 617 Size: 959 493 521 678 411 147 274 1397 Fragments arranged by size: 1397 959 678 521 493 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1093 1123 1093 Size: 30 4850 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 421 421 Size: 4880 BseYI C'CCAG_C Cuts at: 324 672 803 1407 1693 2305 3063 3728 4136 Size: 348 131 604 286 612 758 665 408 Cuts at: 4136 324 Size: 1068 Fragments arranged by size: 1068 758 665 612 604 408 348 286 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1579 1579 Size: 4880 BsiEI CG_ry'CG Cuts at: 104 119 2422 2468 3698 104 Size: 15 2303 46 1230 1286 Fragments arranged by size: 2303 1286 1230 46 15 BsiHKAI G_wGCw'C Cuts at: 155 368 464 631 917 1199 1894 2672 2862 Size: 213 96 167 286 282 695 778 190 Cuts at: 2862 3722 155 Size: 860 1313 Fragments arranged by size: 1313 860 778 695 286 282 213 190 167 96 Bsp1286I G_dGCh'C Cuts at: 155 368 464 631 917 1199 1240 1542 1754 Size: 213 96 167 286 282 41 302 212 Cuts at: 1754 1894 2505 2598 2672 2862 2924 3722 155 Size: 140 611 93 74 190 62 798 1313 Fragments arranged by size: 1313 798 611 302 286 282 213 212 190 167 140 96 93 74 62 41 BspEI T'CCGG_A Cuts at: 617 1576 617 Size: 959 3921 BspHI T'CATG_A Cuts at: 3312 3312 Size: 4880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1601 1930 2144 2446 2827 1601 Size: 329 214 302 381 3654 Fragments arranged by size: 3654 381 329 302 214 BsrBI CCG'CTC Cuts at: 50 3172 3226 4710 4875 50 Size: 3122 54 1484 165 55 Fragments arranged by size: 3122 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 177 2792 177 Size: 2615 2265 BsrFI r'CCGG_y Cuts at: 2878 3059 2878 Size: 181 4699 BssSI C'ACGA_G Cuts at: 457 3151 3859 457 Size: 2694 708 1478 Fragments arranged by size: 2694 1478 708 BstAPI GCAn_nnn'nTGC Cuts at: 372 492 1203 2257 372 Size: 120 711 1054 2995 Fragments arranged by size: 2995 1054 711 120 BstBI TT'CG_AA Cuts at: 3241 3241 Size: 4880 BstXI CCAn_nnnn'nTGG Cuts at: 225 693 1566 225 Size: 468 873 3539 Fragments arranged by size: 3539 873 468 BstYI r'GATC_y Cuts at: 1 822 1082 1905 2372 2395 2730 2976 3282 Size: 821 260 823 467 23 335 246 306 Cuts at: 3282 3294 3380 4432 1 Size: 12 86 1052 449 Fragments arranged by size: 1052 823 821 467 449 335 306 260 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1241 1241 Size: 4880 BtgI C'CryG_G Cuts at: 107 644 1676 107 Size: 537 1032 3311 Fragments arranged by size: 3311 1032 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 129 204 783 2830 3011 3174 129 Size: 75 579 2047 181 163 1835 Fragments arranged by size: 2047 1835 579 181 163 75 BtsI GCAGTG_nn' Cuts at: 366 437 932 2123 366 Size: 71 495 1191 3123 Fragments arranged by size: 3123 1191 495 71 DraI TTT'AAA Cuts at: 1879 1879 Size: 4880 DraIII CAC_nnn'GTG Cuts at: 492 492 Size: 4880 DrdI GACnn_nn'nnGTC Cuts at: 1863 2586 3930 1863 Size: 723 1344 2813 Fragments arranged by size: 2813 1344 723 EaeI y'GGCC_r Cuts at: 116 395 1370 1568 1727 2465 2639 3030 3057 Size: 279 975 198 159 738 174 391 27 Cuts at: 3057 4402 116 Size: 1345 594 Fragments arranged by size: 1345 975 738 594 391 279 198 174 159 27 EagI C'GGCC_G Cuts at: 116 2465 116 Size: 2349 2531 EarI CTCTTCn'nnn_ Cuts at: 562 1216 1618 2903 3113 562 Size: 654 402 1285 210 2329 Fragments arranged by size: 2329 1285 654 402 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 613 3088 3830 3976 4690 613 Size: 2475 742 146 714 803 Fragments arranged by size: 2475 803 742 714 146 EcoICRI GAG'CTC Cuts at: 915 1892 915 Size: 977 3903 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 406 499 715 847 884 901 1246 1525 2705 Size: 93 216 132 37 17 345 279 1180 Cuts at: 2705 3137 3490 4152 406 Size: 432 353 662 1134 Fragments arranged by size: 1180 1134 662 432 353 345 279 216 132 93 37 17 EcoO109I rG'GnC_Cy Cuts at: 245 300 831 931 1698 1750 2019 245 Size: 55 531 100 767 52 269 3106 Fragments arranged by size: 3106 767 531 269 100 55 52 EcoRI G'AATT_C Cuts at: 1884 1884 Size: 4880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2899 2899 Size: 4880 FspI TGC'GCA Cuts at: 1687 2661 1687 Size: 974 3906 HaeII r_GCGC'y Cuts at: 2314 2562 3792 4563 2314 Size: 248 1230 771 2631 Fragments arranged by size: 2631 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 831 863 1093 1125 2722 2752 2754 2784 831 Size: 32 230 32 1597 30 2 30 2927 Fragments arranged by size: 2927 1597 230 32 32 30 30 2 HincII GTy'rAC Cuts at: 1919 1919 Size: 4880 KasI G'GCGC_C Cuts at: 2310 2558 2310 Size: 248 4632 KpnI G_GTAC'C Cuts at: 1900 1900 Size: 4880 MluI A'CGCG_T Cuts at: 4313 4313 Size: 4880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 761 3639 3823 761 Size: 2878 184 1818 Fragments arranged by size: 2878 1818 184 MscI TGG'CCA Cuts at: 397 1372 1570 1729 2641 397 Size: 975 198 159 912 2636 Fragments arranged by size: 2636 975 912 198 159 MslI CAynn'nnrTG Cuts at: 148 643 727 1472 1624 2869 2996 148 Size: 495 84 745 152 1245 127 2032 Fragments arranged by size: 2032 1245 745 495 152 127 84 MspA1I CmG'CkG Cuts at: 324 384 862 1051 1224 1305 1407 1991 2305 Size: 60 478 189 173 81 102 584 314 Cuts at: 2305 2665 3449 3694 324 Size: 360 784 245 1510 Fragments arranged by size: 1510 784 584 478 360 314 245 189 173 102 81 60 NaeI GCC'GGC Cuts at: 3061 3061 Size: 4880 NarI GG'CG_CC Cuts at: 2311 2559 2311 Size: 248 4632 NcoI C'CATG_G Cuts at: 107 644 107 Size: 537 4343 NgoMIV G'CCGG_C Cuts at: 3059 3059 Size: 4880 NspI r_CATG'y Cuts at: 1129 1389 1671 1933 2433 4036 1129 Size: 260 282 262 500 1603 1973 Fragments arranged by size: 1973 1603 500 282 262 260 PciI A'CATG_T Cuts at: 1125 4032 1125 Size: 2907 1973 PflMI CCAn_nnn'nTGG Cuts at: 1824 1824 Size: 4880 PfoI T'CCnGG_A Cuts at: 1757 1757 Size: 4880 PmeI GTTT'AAAC Cuts at: 1879 1879 Size: 4880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 613 645 1246 1278 3326 3358 613 Size: 32 601 32 2048 32 2135 Fragments arranged by size: 2135 2048 601 32 32 32 PpuMI rG'GwC_Cy Cuts at: 245 300 1698 245 Size: 55 1398 3427 Fragments arranged by size: 3427 1398 55 PspOMI G'GGCC_C Cuts at: 1538 1750 1538 Size: 212 4668 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 321 353 321 Size: 32 4848 PstI C_TGCA'G Cuts at: 1742 1927 2612 1742 Size: 185 685 4010 Fragments arranged by size: 4010 685 185 PvuI CG_AT'CG Cuts at: 104 104 Size: 4880 PvuII CAG'CTG Cuts at: 324 1051 1224 1305 1407 2305 2665 324 Size: 727 173 81 102 898 360 2539 Fragments arranged by size: 2539 898 727 360 173 102 81 RsrII CG'GwC_CG Cuts at: 3075 3075 Size: 4880 SacI G_AGCT'C Cuts at: 917 1894 917 Size: 977 3903 SalI G'TCGA_C Cuts at: 1917 1917 Size: 4880 SanDI GG'GwC_CC Cuts at: 1698 1698 Size: 4880 SapI GCTCTTCn'nnn_ Cuts at: 1618 2903 3113 1618 Size: 1285 210 3385 Fragments arranged by size: 3385 1285 210 SbfI CC_TGCA'GG Cuts at: 1927 1927 Size: 4880 ScaI AGT'ACT Cuts at: 613 4397 613 Size: 3784 1096 SexAI A'CCwGG_T Cuts at: 953 953 Size: 4880 SfcI C'TryA_G Cuts at: 33 1738 1923 2608 3576 3767 33 Size: 1705 185 685 968 191 1146 Fragments arranged by size: 1705 1146 968 685 191 185 SfoI GGC'GCC Cuts at: 2312 2560 2312 Size: 248 4632 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4880 SmaI CCC'GGG Cuts at: 1902 1902 Size: 4880 SmlI C'TyrA_G Cuts at: 2035 2950 3664 3926 2035 Size: 915 714 262 2989 Fragments arranged by size: 2989 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4880 SphI G_CATG'C Cuts at: 1933 2433 1933 Size: 500 4380 StyI C'CwwG_G Cuts at: 107 224 231 644 686 1559 2014 2368 107 Size: 117 7 413 42 873 455 354 2619 Fragments arranged by size: 2619 873 455 413 354 117 42 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2463 2596 3055 3264 2463 Size: 133 459 209 4079 Fragments arranged by size: 4079 459 209 133 TatI w'GTAC_w Cuts at: 611 1382 4395 611 Size: 771 3013 1096 Fragments arranged by size: 3013 1096 771 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 237 269 1339 1371 4397 4429 237 Size: 32 1070 32 3026 32 688 Fragments arranged by size: 3026 1070 688 32 32 32 Tth111I GACn'n_nGTC Cuts at: 706 955 2677 706 Size: 249 1722 2909 Fragments arranged by size: 2909 1722 249 XbaI T'CTAG_A Cuts at: 1911 1911 Size: 4880 XcmI CCAnnnn_n'nnnnTGG Cuts at: 222 951 1335 2375 222 Size: 729 384 1040 2727 Fragments arranged by size: 2727 1040 729 384 XmaI C'CCGG_G Cuts at: 1900 1900 Size: 4880 ZraI GAC'GTC Cuts at: 1830 1830 Size: 4880 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI