Restriction map for pF1KB3129
 (Circular) (Six-base) MAPSORT of: pF1KB3129.seq  Check: 7284  from: 1  to: 3830



pF1KB3129  3830 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    668     668 
   Size:       3830
 
AccI GT'mk_AC

Cuts at:    868     868 
   Size:       3830
 
Acc65I G'GTAC_C

Cuts at:    846     846 
   Size:       3830
 
AclI AA'CG_TT

Cuts at:   3670    3670 
   Size:       3830
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    504     778    1655    2087    2440    3102     504 
   Size:        274     877     432     353     662    1232

  Fragments arranged by size: 

                1232     877     662     432     353     274
 
AflIII A'CryG_T

Cuts at:   1182    2982    3263    1182 
   Size:       1800     281    1749

  Fragments arranged by size: 

                1800    1749     281
 
AleI CACnn'nnGTG

Cuts at:    505     505 
   Size:       3830
 
AlwNI CAG_nnn'CTG

Cuts at:   1135    2573    1135 
   Size:       1438    2392
 
ApaLI G'TGCA_C

Cuts at:   2668    2668 
   Size:       3830
 
ApoI r'AATT_y

Cuts at:    834    3183    3439     834 
   Size:       2349     256    1225

  Fragments arranged by size: 

                2349    1225     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3830
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3830
 
AvaI C'yCGr_G

Cuts at:    225     265     293     850     225 
   Size:         40      28     557    3205

  Fragments arranged by size: 

                3205     557      40      28
 
BamHI G'GATC_C

Cuts at:    671     855     671 
   Size:        184    3646
 
BanI G'GyrC_C

Cuts at:    214     343     372     656     805     846    1260    1508    1543 
   Size:        129      29     284     149      41     414     248      35

Cuts at:   1543     214 
   Size:       2501

  Fragments arranged by size: 

                2501     414     284     248     149     129      41      35
                  29
 
BanII G_rGCy'C

Cuts at:    518     844    1874     518 
   Size:        326    1030    2474

  Fragments arranged by size: 

                2474    1030     326
 
BbeI G_GCGC'C

Cuts at:    218     347    1264    1512     218 
   Size:        129     917     248    2536

  Fragments arranged by size: 

                2536     917     248     129
 
BbsI GAAGACnn'nnnn_

Cuts at:    766     766 
   Size:       3830
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    181     215     181 
   Size:         34    3796
 
BciVI GTATCCnnnnn_n'

Cuts at:   1728    2784    3830    1728 
   Size:       1056    1046    1728

  Fragments arranged by size: 

                1728    1056    1046
 
BclI T'GATC_A

Cuts at:   1350    1350 
   Size:       3830
 
BglI GCCn_nnn'nGGC

Cuts at:    407     407 
   Size:       3830
 
BglII A'GATC_T

Cuts at:   1345    3382    1345 
   Size:       2037    1793
 
BlpI GC'TnA_GC

Cuts at:    364     942     364 
   Size:        578    3252
 
Bme1580I G_kGCm'C

Cuts at:    272    1455    1548    2672     272 
   Size:       1183      93    1124    1430

  Fragments arranged by size: 

                1430    1183    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    546    1042    1123    1458    3602     546 
   Size:        496      81     335    2144     774

  Fragments arranged by size: 

                2144     774     496     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2197    2197 
   Size:       3830
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1006    1885    2599    2897    1006 
   Size:        879     714     298    1939

  Fragments arranged by size: 

                1939     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3534    3534 
   Size:       3830
 
BsaAI yAC'GTr

Cuts at:   1183    1183 
   Size:       3830
 
BsaBI GATnn'nnATC

Cuts at:      6    1344    3381       6 
   Size:       1338    2037     455

  Fragments arranged by size: 

                2037    1338     455
 
BsaHI Gr'CG_yC

Cuts at:    215     344     472    1261    1509    2211    3707     215 
   Size:        129     128     789     248     702    1496     338

  Fragments arranged by size: 

                1496     789     702     338     248     129     128
 
BsaWI w'CCGG_w

Cuts at:   1019    1540    2218    2629    2776    3050    1019 
   Size:        521     678     411     147     274    1799

  Fragments arranged by size: 

                1799     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    403     433     403 
   Size:         30    3800
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    246     526     246 
   Size:        280    3550
 
BseYI C'CCAG_C

Cuts at:    118     260     689     718    1255    2013    2678    3086     118 
   Size:        142     429      29     537     758     665     408     862

  Fragments arranged by size: 

                 862     758     665     537     429     408     142      29
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    448     646     448 
   Size:        198    3632
 
BsiEI CG_ry'CG

Cuts at:    104     305    1372    1418    2648     104 
   Size:        201    1067      46    1230    1286

  Fragments arranged by size: 

                1286    1230    1067     201      46
 
BsiHKAI G_wGCw'C

Cuts at:    518     844    1622    1812    2672     518 
   Size:        326     778     190     860    1676

  Fragments arranged by size: 

                1676     860     778     326     190
 
Bsp1286I G_dGCh'C

Cuts at:    272     518     844    1455    1548    1622    1812    1874    2672 
   Size:        246     326     611      93      74     190      62     798

Cuts at:   2672     272 
   Size:       1430

  Fragments arranged by size: 

                1430     798     611     326     246     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   2262    2262 
   Size:       3830
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    668     880    1094    1396    1777     668 
   Size:        212     214     302     381    2721

  Fragments arranged by size: 

                2721     381     302     214     212
 
BsrBI CCG'CTC

Cuts at:     50    2122    2176    3660    3825      50 
   Size:       2072      54    1484     165      55

  Fragments arranged by size: 

                2072    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1742    1742 
   Size:       3830
 
BsrFI r'CCGG_y

Cuts at:    406     653    1828    2009     406 
   Size:        247    1175     181    2227

  Fragments arranged by size: 

                2227    1175     247     181
 
BssSI C'ACGA_G

Cuts at:   2101    2809    2101 
   Size:        708    3122
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1207    1207 
   Size:       3830
 
BstBI TT'CG_AA

Cuts at:   2191    2191 
   Size:       3830
 
BstXI CCAn_nnnn'nTGG

Cuts at:    507     507 
   Size:       3830
 
BstYI r'GATC_y

Cuts at:      1     671     855    1322    1345    1680    1926    2232    2244 
   Size:        670     184     467      23     335     246     306      12

Cuts at:   2244    2330    3382       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1052     670     467     449     335     306     246     184
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     350     500     107 
   Size:        243     150    3437

  Fragments arranged by size: 

                3437     243     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    609    1780    1961    2124     609 
   Size:       1171     181     163    2315

  Fragments arranged by size: 

                2315    1171     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1073    1073 
   Size:       3830
 
DraI TTT'AAA

Cuts at:    829     829 
   Size:       3830
 
DrdI GACnn_nn'nnGTC

Cuts at:   1536    2880    1536 
   Size:       1344    2486
 
EaeI y'GGCC_r

Cuts at:   1415    1589    1980    2007    3352    1415 
   Size:        174     391      27    1345    1893

  Fragments arranged by size: 

                1893    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1415    1415 
   Size:       3830
 
EarI CTCTTCn'nnn_

Cuts at:    523    1853    2063     523 
   Size:       1330     210    2290

  Fragments arranged by size: 

                2290    1330     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    388    2038    2780    2926    3640     388 
   Size:       1650     742     146     714     578

  Fragments arranged by size: 

                1650     742     714     578     146
 
EcoICRI GAG'CTC

Cuts at:    516     842     516 
   Size:        326    3504
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    504     778    1655    2087    2440    3102     504 
   Size:        274     877     432     353     662    1232

  Fragments arranged by size: 

                1232     877     662     432     353     274
 
EcoNI CCTnn'n_nnAGG

Cuts at:    400     400 
   Size:       3830
 
EcoO109I rG'GnC_Cy

Cuts at:    297     311     355     969     297 
   Size:         14      44     614    3158

  Fragments arranged by size: 

                3158     614      44      14
 
EcoRI G'AATT_C

Cuts at:    834     834 
   Size:       3830
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1849    1849 
   Size:       3830
 
FspI TGC'GCA

Cuts at:   1611    1611 
   Size:       3830
 
HaeII r_GCGC'y

Cuts at:    218     347    1264    1512    2742    3513     218 
   Size:        129     917     248    1230     771     535

  Fragments arranged by size: 

                1230     917     771     535     248     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    403     435     779     811    1672    1702    1704    1734     403 
   Size:         32     344      32     861      30       2      30    2499

  Fragments arranged by size: 

                2499     861     344      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    594     869     594 
   Size:        275    3555
 
KasI G'GCGC_C

Cuts at:    214     343    1260    1508     214 
   Size:        129     917     248    2536

  Fragments arranged by size: 

                2536     917     248     129
 
KpnI G_GTAC'C

Cuts at:    850     850 
   Size:       3830
 
MluI A'CGCG_T

Cuts at:   3263    3263 
   Size:       3830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2589    2773    2589 
   Size:        184    3646
 
MscI TGG'CCA

Cuts at:   1591    1591 
   Size:       3830
 
MslI CAynn'nnrTG

Cuts at:    505     580    1819    1946     505 
   Size:         75    1239     127    2389

  Fragments arranged by size: 

                2389    1239     127      75
 
MspA1I CmG'CkG

Cuts at:    321     352     462     941    1255    1615    2399    2644     321 
   Size:         31     110     479     314     360     784     245    1507

  Fragments arranged by size: 

                1507     784     479     360     314     245     110      31
            
 
NaeI GCC'GGC

Cuts at:    408    2011     408 
   Size:       1603    2227
 
NarI GG'CG_CC

Cuts at:    215     344    1261    1509     215 
   Size:        129     917     248    2536

  Fragments arranged by size: 

                2536     917     248     129
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3830
 
NgoMIV G'CCGG_C

Cuts at:    406    2009     406 
   Size:       1603    2227
 
NspI r_CATG'y

Cuts at:    726     883    1383    2986     726 
   Size:        157     500    1603    1570

  Fragments arranged by size: 

                1603    1570     500     157
 
PasI CC'CwG_GG

Cuts at:    801     801 
   Size:       3830
 
PciI A'CATG_T

Cuts at:   2982    2982 
   Size:       3830
 
PfoI T'CCnGG_A

Cuts at:    674     674 
   Size:       3830
 
PmeI GTTT'AAAC

Cuts at:    829     829 
   Size:       3830
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2276    2308    2276 
   Size:         32    3798
 
PpuMI rG'GwC_Cy

Cuts at:    297     311     297 
   Size:         14    3816
 
PsiI TTA'TAA

Cuts at:    170     170 
   Size:       3830
 
PstI C_TGCA'G

Cuts at:    326     877    1562     326 
   Size:        551     685    2594

  Fragments arranged by size: 

                2594     685     551
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3830
 
PvuII CAG'CTG

Cuts at:    462    1255    1615     462 
   Size:        793     360    2677

  Fragments arranged by size: 

                2677     793     360
 
RsrII CG'GwC_CG

Cuts at:   2025    2025 
   Size:       3830
 
SacI G_AGCT'C

Cuts at:    518     844     518 
   Size:        326    3504
 
SacII CC_GC'GG

Cuts at:    353     353 
   Size:       3830
 
SalI G'TCGA_C

Cuts at:    867     867 
   Size:       3830
 
SanDI GG'GwC_CC

Cuts at:    297     297 
   Size:       3830
 
SapI GCTCTTCn'nnn_

Cuts at:    523    1853    2063     523 
   Size:       1330     210    2290

  Fragments arranged by size: 

                2290    1330     210
 
SbfI CC_TGCA'GG

Cuts at:    877     877 
   Size:       3830
 
ScaI AGT'ACT

Cuts at:   3347    3347 
   Size:       3830
 
SfcI C'TryA_G

Cuts at:     33     322     873    1558    2526    2717      33 
   Size:        289     551     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     551     289     191
 
SfoI GGC'GCC

Cuts at:    216     345    1262    1510     216 
   Size:        129     917     248    2536

  Fragments arranged by size: 

                2536     917     248     129
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3830
 
SmaI CCC'GGG

Cuts at:    227     267     295     852     227 
   Size:         40      28     557    3205

  Fragments arranged by size: 

                3205     557      40      28
 
SmlI C'TyrA_G

Cuts at:    985    1900    2614    2876     985 
   Size:        915     714     262    1939

  Fragments arranged by size: 

                1939     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3830
 
SphI G_CATG'C

Cuts at:    726     883    1383     726 
   Size:        157     500    3173

  Fragments arranged by size: 

                3173     500     157
 
SrfI GCCC'GGGC

Cuts at:    267     267 
   Size:       3830
 
StyI C'CwwG_G

Cuts at:    107     964    1318     107 
   Size:        857     354    2619

  Fragments arranged by size: 

                2619     857     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    793    1413    1546    2005    2214     793 
   Size:        620     133     459     209    2409

  Fragments arranged by size: 

                2409     620     459     209     133
 
TatI w'GTAC_w

Cuts at:    158    3345     158 
   Size:       3187     643
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    264     296     368     400    3347    3379     264 
   Size:         32      72      32    2947      32     715

  Fragments arranged by size: 

                2947     715      72      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    301    1627     301 
   Size:       1326    2504
 
XbaI T'CTAG_A

Cuts at:    861     861 
   Size:       3830
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1325    1325 
   Size:       3830
 
XmaI C'CCGG_G

Cuts at:    225     265     293     850     225 
   Size:         40      28     557    3205

  Fragments arranged by size: 

                3205     557      40      28
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI     PsiI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI     BpmI
   Bpu10I    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmlI    PshAI   PspOMI    PspXI     PsrI    SexAI
     SfiI    SgrAI    SnaBI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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