Restriction map for pF1KB3109
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BamHI       BbeI        BbrPI       
BbvCI       BglI        BglII       BlnI        BlpI        
Bpu1102I    BseAI       BsiWI       Bsp1407I    BspEI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     CelII       CpoI        DraI        Eco47III    
EcoRV       EcoT22I     FseI        HaeII       HpaI        
KasI        KpnI        KspI        MfeI        MroI        
MunI        NarI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    RsrII       SacI        SacII       
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AatII        1610  GACGTC               GACGT^C
AccI         1520  GTCGAC               GT^[AC][GT]AC
AhdI          423  GACATTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         423  GACATTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          88  TGCGCA               TGC^GCA
BclI         3563  TGATCA               T^GATCA
BcnI         4997  CCGGG                CC^[GC]GG
BsaWI        1733  ACCGGA               [AT]^CCGG[AT]
BsmBI        3418  GAGACG               CGTCTC(1/5)
BspDI        2101  ATCGAT               AT^CGAT
BsrBI        1714  GAGCGG               CCGCTC(-3/-3)
BssHII         57  GCGCGC               G^CGCGC
ClaI         2101  ATCGAT               AT^CGAT
DrdI         4342  GACCAAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      423  GACATTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         388  GAATTC               G^AATTC
FbaI         3563  TGATCA               T^GATCA
FspI           88  TGCGCA               TGC^GCA
HincII       1520  GTCGAC               GT[CT]^[GA]AC
HindII       1520  GTCGAC               GT[CT]^[GA]AC
HindIII       145  AAGCTT               A^AGCTT
MluI         4928  ACGCGT               A^CGCGT
NciI         4997  CCGGG                CC^[GC]GG
NdeI          231  CATATG               CA^TATG
NheI           18  GCTAGC               G^CTAGC
PvuI          454  CGATCG               CGAT^CG
SalI         1520  GTCGAC               G^TCGAC
SphI         3314  GCATGC               GCATG^C
XbaI         2217  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII       1195  ACGTGT               A^C[GA][CT]GT
             4928  ACGCGT              
Alw44I       2417  GTGCAC               G^TGCAC
             3492  GTGCAC              
AocI         2329  CCTCAGG              CC^T[ACGT]AGG
             2876  CCTCAGG             
ApaLI        2417  GTGCAC               G^TGCAC
             3492  GTGCAC              
BanI         3100  GGCACC               G^G[CT][GA]CC
             3946  GGTGCC              
BsaAI        1194  TACGTG               [CT]AC^GT[GA]
             4640  TACGTG              
BsaBI         247  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              753  GATGGAAATC          
BsmI         2320  GAATGC               GAATGC(1/-1)
             4005  GAATGC              
BspHI         255  TCATGA               T^CATGA
             4052  TCATGA              
BstAPI       3341  GCAGCGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3527  GCACACCCTGC         
BstEII        153  GGTGACC              G^GT[ACGT]ACC
             2175  GGTCACC             
BstPI         153  GGTGACC              G^GT[ACGT]ACC
             2175  GGTCACC             
Bsu36I       2329  CCTCAGG              CC^T[ACGT]AGG
             2876  CCTCAGG             
DraIII       3425  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3479  CACGCTGTG           
EagI          132  CGGCCG               C^GGCCG
             2002  CGGCCG              
EarI         3358  CTCTTC               CTCTTC(1/4)
             5128  GAAGAG              
EclXI         132  CGGCCG               C^GGCCG
             2002  CGGCCG              
Eco52I        132  CGGCCG               C^GGCCG
             2002  CGGCCG              
Eco81I       2329  CCTCAGG              CC^T[ACGT]AGG
             2876  CCTCAGG             
EcoNI         967  CCTGCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3060  CCTCCGGCAGG         
EcoO65I       153  GGTGACC              G^GT[ACGT]ACC
             2175  GGTCACC             
Ksp632I      3358  CTCTTC               CTCTTC(1/4)
             5128  GAAGAG              
MamI          247  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              753  GATGGAAATC          
PflMI        2632  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3209  CCAATACCTGG         
RcaI          255  TCATGA               T^CATGA
             4052  TCATGA              
SexAI        1632  ACCTGGT              A^CC[AT]GGT
             1955  ACCAGGT             
Van91I       2632  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3209  CCAATACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflII       AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanII       BbsI        BbvI        BcgI        BciVI       
BfaI        BfrI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmnI        
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