Restriction map for pF1KB3104
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AhdI        
AocI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbvCI       BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsiWI       Bsp1407I    BspDI       BsrBI       BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MluI        MluNI       MscI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          456  GTATAC               GT^[AC][GT]AC
AccIII        735  TCCGGA               T^CCGGA
AcyI         1048  GACGCC               G[GA]^CG[CT]C
AgeI         2321  ACCGGT               A^CCGGT
ApaI          555  GGGCCC               GGGCC^C
Asp700I      2191  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI         1702  GGCACC               G^G[CT][GA]CC
BclI         2168  TGATCA               T^GATCA
BglI          557  GCCCTAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1048  GACGCC               G[GA]^CG[CT]C
BseAI         735  TCCGGA               T^CCGGA
BsiEI        1797  CGGCCG               CG[GA][CT]^CG
BsmI         2347  GAATGC               GAATGC(1/-1)
BspEI         735  TCCGGA               T^CCGGA
BspHI         243  TCATGA               T^CATGA
BsrDI         527  GCAATG               GCAATG(2/0)
Bst1107I      456  GTATAC               GTA^TAC
BstZ17I       456  GTATAC               GTA^TAC
DraIII       1834  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1797  CGGCCG               C^GGCCG
EclXI        1797  CGGCCG               C^GGCCG
Eco52I       1797  CGGCCG               C^GGCCG
EcoRI         277  GAATTC               G^AATTC
FbaI         2168  TGATCA               T^GATCA
Hin1I        1048  GACGCC               G[GA]^CG[CT]C
HindIII       535  AAGCTT               A^AGCTT
MfeI          104  CAATTG               C^AATTG
MroI          735  TCCGGA               T^CCGGA
MunI          104  CAATTG               C^AATTG
PinAI        2321  ACCGGT               A^CCGGT
PvuII         571  CAGCTG               CAG^CTG
RcaI          243  TCATGA               T^CATGA
SacI          793  GAGCTC               GAGCT^C
ScaI          953  AGTACT               AGT^ACT
SexAI        1406  ACCTGGT              A^CC[AT]GGT
SfiI          556  GGCCCTAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1707  CCCGGG               CCC^GGG
SmlI         1468  CTCAAG               C^T[CT][GA]AG
StuI         1094  AGGCCT               AGG^CCT
XcmI          905  CCACCCATGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1707  CCCGGG               C^CCGGG
XmaI         1707  CCCGGG               C^CCGGG
XmnI         2191  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Alw44I        608  GTGCAC               G^TGCAC
             1504  GTGCAC              
Aor51HI        22  AGCGCT               AGC^GCT
             1432  AGCGCT              
ApaLI         608  GTGCAC               G^TGCAC
             1504  GTGCAC              
AvaI          250  CCCGAG               C^[CT]CG[GA]G
             1707  CCCGGG              
BamHI         495  GGATCC               G^GATCC
             2357  GGATCC              
BanII         555  GGGCCC               G[GA]GC[CT]^C
              793  GAGCTC              
BbsI          229  GAAGAC               GAAGAC(2/6)
              708  GAAGAC              
BcgI          743  GCACGAGACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              986  GCACCAAGGTCG        
BfaI         1285  CTAG                 C^TAG
             1540  CTAG                
BglII         216  AGATCT               A^GATCT
             1310  AGATCT              
BpuAI         229  GAAGAC               GAAGAC(2/6)
              708  GAAGAC              
BsaI          639  GGTCTC               GGTCTC(1/5)
              731  GGTCTC              
BsaWI         735  TCCGGA               [AT]^CCGG[AT]
             2321  ACCGGT              
BsmBI        1344  GAGACG               CGTCTC(1/5)
             1750  GAGACG              
BsoBI         250  CCCGAG               C^[CT]CG[GA]G
             1707  CCCGGG              
BspLU11I      612  ACATGT               A^CATGT
             1554  ACATGT              
BsrFI         808  ACCGGC               [GA]^CCGG[CT]
             2321  ACCGGT              
BssSI         744  CACGAG               CACGAG(-5/-1)
             1078  CACGAG              
BstXI         629  CCATCCGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1364  CCAACACGGTGG        
Cac8I        1650  GCCAGC               GC[ACGT]^[ACGT]GC
             1856  GCTTGC              
Cfr10I        808  ACCGGC               [GA]^CCGG[CT]
             2321  ACCGGT              
EarI          220  CTCTTC               CTCTTC(1/4)
              694  GAAGAG              
Eco47III       22  AGCGCT               AGC^GCT
             1432  AGCGCT              
HaeII          22  AGCGCT               [GA]GCGC^[CT]
             1432  AGCGCT              
HgaI         1048  GACGC                GACGC(5/10)
             1923  GACGC               
Ksp632I       220  CTCTTC               CTCTTC(1/4)
              694  GAAGAG              
MaeI         1285  CTAG                 C^TAG
             1540  CTAG                
NcoI          965  CCATGG               C^CATGG
             2091  CCATGG              
NspI          612  ACATGT               [GA]CATG^[CT]
             1554  ACATGT              
PflMI         909  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1446  CCATAGCATGG         
PleI          749  GACTC                GAGTC(4/5)
             2138  GAGTC               
PpuMI        1331  AGGACCT              [GA]G^G[AT]CC[CT]
             1479  AGGTCCT             
TaqI         1736  TCGA                 T^CGA
             2136  TCGA                
TfiI          367  GAATC                G^A[AT]TC
              772  GATTC               
TthHB8I      1736  TCGA                 T^CGA
             2136  TCGA                
Van91I        909  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1446  CCATAGCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com