Restriction map for pF1KB3104
 (Circular) (Six-base) MAPSORT of: pF1KB3104.seq  Check: 9359  from: 1  to: 5522



pF1KB3104  5522 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2131    2131 
   Size:       5522
 
AccI GT'mk_AC

Cuts at:    565    2560     565 
   Size:       1995    3527
 
Acc65I G'GTAC_C

Cuts at:   2538    2538 
   Size:       5522
 
AclI AA'CG_TT

Cuts at:   5362    5362 
   Size:       5522
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    315     631    1521    1533    1774    2029    2445    3347    3779 
   Size:        316     890      12     241     255     416     902     432

Cuts at:   3779    4132    4794     315 
   Size:        353     662    1043

  Fragments arranged by size: 

                1043     902     890     662     432     416     353     316
                 255     241      12
 
AfeI AGC'GCT

Cuts at:    132    1542     132 
   Size:       1410    4112
 
AflIII A'CryG_T

Cuts at:    720     990    1133    1662    2874    4674    4955     720 
   Size:        270     143     529    1212    1800     281    1287

  Fragments arranged by size: 

                1800    1287    1212     529     281     270     143
 
AgeI A'CCGG_T

Cuts at:   2429    2429 
   Size:       5522
 
AleI CACnn'nnGTG

Cuts at:    834    1075     834 
   Size:        241    5281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2255    2287    2255 
   Size:         32    5490
 
AlwNI CAG_nnn'CTG

Cuts at:    168     791    1752    2274    2424    2827    4265     168 
   Size:        623     961     522     150     403    1438    1425

  Fragments arranged by size: 

                1438    1425     961     623     522     403     150
 
ApaI G_GGCC'C

Cuts at:    667     667 
   Size:       5522
 
ApaLI G'TGCA_C

Cuts at:    716    1612    4360     716 
   Size:        896    2748    1878

  Fragments arranged by size: 

                2748    1878     896
 
ApoI r'AATT_y

Cuts at:    347     385     590    1297    2303    2526    4875    5131     347 
   Size:         38     205     707    1006     223    2349     256     738

  Fragments arranged by size: 

                2349    1006     738     707     256     223     205      38
            
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5522
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5522
 
AvaI C'yCGr_G

Cuts at:    358    1815    2542     358 
   Size:       1457     727    3338

  Fragments arranged by size: 

                3338    1457     727
 
BamHI G'GATC_C

Cuts at:    603    2465    2547     603 
   Size:       1862      82    3578

  Fragments arranged by size: 

                3578    1862      82
 
BanI G'GyrC_C

Cuts at:   1810    2538    2952    3200    3235    1810 
   Size:        728     414     248      35    4097

  Fragments arranged by size: 

                4097     728     414     248      35
 
BanII G_rGCy'C

Cuts at:    667     905    2536    3566     667 
   Size:        238    1631    1030    2623

  Fragments arranged by size: 

                2623    1631    1030     238
 
BbeI G_GCGC'C

Cuts at:   2956    3204    2956 
   Size:        248    5274
 
BbsI GAAGACnn'nnnn_

Cuts at:    344     823     344 
   Size:        479    5043
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    840     874    1083    1117     840 
   Size:         34     209      34    5245

  Fragments arranged by size: 

                5245     209      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3420    4476    5522    3420 
   Size:       1056    1046    3420

  Fragments arranged by size: 

                3420    1056    1046
 
BclI T'GATC_A

Cuts at:   2276    3042    2276 
   Size:        766    4756
 
BglI GCCn_nnn'nGGC

Cuts at:    671     671 
   Size:       5522
 
BglII A'GATC_T

Cuts at:    324    1418    3037    5074     324 
   Size:       1094    1619    2037     772

  Fragments arranged by size: 

                2037    1619    1094     772
 
BlpI GC'TnA_GC

Cuts at:   2634    2634 
   Size:       5522
 
Bme1580I G_kGCm'C

Cuts at:    667     720    1616    3147    3240    4364     667 
   Size:         53     896    1531      93    1124    1825

  Fragments arranged by size: 

                1825    1531    1124     896      93      53
 
BmgBI CAC'GTC

Cuts at:   1380    1380 
   Size:       5522
 
BmrI ACTGGGnnnn_n'

Cuts at:   1383    2734    2815    3150    5294    1383 
   Size:       1351      81     335    2144    1611

  Fragments arranged by size: 

                2144    1611    1351     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    200    3889     200 
   Size:       3689    1833
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    491    1045    1990    2391     491 
   Size:        554     945     401    3622

  Fragments arranged by size: 

                3622     945     554     401
 
Bpu10I CC'TnA_GC

Cuts at:    583    1429    2071     583 
   Size:        846     642    4034

  Fragments arranged by size: 

                4034     846     642
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1561    2698    3577    4291    4589    1561 
   Size:       1137     879     714     298    2494

  Fragments arranged by size: 

                2494    1137     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    753     845    5226     753 
   Size:         92    4381    1049

  Fragments arranged by size: 

                4381    1049      92
 
BsaAI yAC'GTr

Cuts at:   2875    2875 
   Size:       5522
 
BsaBI GATnn'nnATC

Cuts at:      6    3036    5073       6 
   Size:       3030    2037     455

  Fragments arranged by size: 

                3030    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1157    2953    3201    3903    5399    1157 
   Size:       1796     248     702    1496    1280

  Fragments arranged by size: 

                1796    1496    1280     702     248
 
BsaWI w'CCGG_w

Cuts at:    843    2429    2711    3232    3910    4321    4468    4742     843 
   Size:       1586     282     521     678     411     147     274    1623

  Fragments arranged by size: 

                1623    1586     678     521     411     282     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    904     934     904 
   Size:         30    5492
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    820    1987    2521    2524     820 
   Size:       1167     534       3    3818

  Fragments arranged by size: 

                3818    1167     534       3
 
BseYI C'CCAG_C

Cuts at:    976    1325    1853    1959    2088    2947    3705    4370    4778 
   Size:        349     528     106     129     859     758     665     408

Cuts at:   4778     976 
   Size:       1720

  Fragments arranged by size: 

                1720     859     758     665     528     408     349     129
                 106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    169    1158    1512    1924     169 
   Size:        989     354     412    3767

  Fragments arranged by size: 

                3767     989     412     354
 
BsiEI CG_ry'CG

Cuts at:    104    1908    3064    3110    4340     104 
   Size:       1804    1156      46    1230    1286

  Fragments arranged by size: 

                1804    1286    1230    1156      46
 
BsiHKAI G_wGCw'C

Cuts at:    720     905    1435    1616    2077    2536    3314    3504    4364 
   Size:        185     530     181     461     459     778     190     860

Cuts at:   4364     720 
   Size:       1878

  Fragments arranged by size: 

                1878     860     778     530     461     459     190     185
                 181
 
BsmI GAATG_Cn'

Cuts at:   2461    2461 
   Size:       5522
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1446    1852    1446 
   Size:        406    5116
 
Bsp1286I G_dGCh'C

Cuts at:    667     720     905    1435    1616    2077    2536    3147    3240 
   Size:         53     185     530     181     461     459     611      93

Cuts at:   3240    3314    3504    3566    4364     667 
   Size:         74     190      62     798    1825

  Fragments arranged by size: 

                1825     798     611     530     461     459     190     185
                 181      93      74      62      53
 
BspEI T'CCGG_A

Cuts at:    843     843 
   Size:       5522
 
BspHI T'CATG_A

Cuts at:    351    3954     351 
   Size:       3603    1919
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1451    1745    2131    2572    2786    3088    3469    1451 
   Size:        294     386     441     214     302     381    3504

  Fragments arranged by size: 

                3504     441     386     381     302     294     214
 
BsrBI CCG'CTC

Cuts at:     50    3814    3868    5352    5517      50 
   Size:       3764      54    1484     165      55

  Fragments arranged by size: 

                3764    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    642    3434     642 
   Size:       2792    2730
 
BsrFI r'CCGG_y

Cuts at:    916    2429    3520    3701     916 
   Size:       1513    1091     181    2737

  Fragments arranged by size: 

                2737    1513    1091     181
 
BssSI C'ACGA_G

Cuts at:    852    1186    3793    4501     852 
   Size:        334    2607     708    1873

  Fragments arranged by size: 

                2607    1873     708     334
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2899    2899 
   Size:       5522
 
BstBI TT'CG_AA

Cuts at:   3883    3883 
   Size:       5522
 
BstXI CCAn_nnnn'nTGG

Cuts at:    744    1479     744 
   Size:        735    4787
 
BstYI r'GATC_y

Cuts at:      1     324     603    1388    1418    2465    2547    3014    3037 
   Size:        323     279     785      30    1047      82     467      23

Cuts at:   3037    3372    3618    3924    3936    4022    5074       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052    1047     785     467     449     335     323     306
                 279     246      86      82      30      23      12
 
BstZ17I GTA'TAC

Cuts at:    566     566 
   Size:       5522
 
BtgI C'CryG_G

Cuts at:   1048    1073    1163    1878    1908    2199    1048 
   Size:         25      90     715      30     291    4371

  Fragments arranged by size: 

                4371     715     291      90      30      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    810    3472    3653    3816     810 
   Size:       2662     181     163    2516

  Fragments arranged by size: 

                2662    2516     181     163
 
BtsI GCAGTG_nn'

Cuts at:    217    1504    2288    2765     217 
   Size:       1287     784     477    2974

  Fragments arranged by size: 

                2974    1287     784     477
 
DraI TTT'AAA

Cuts at:   2521    2521 
   Size:       5522
 
DraIII CAC_nnn'GTG

Cuts at:   1947    1947 
   Size:       5522
 
DrdI GACnn_nn'nnGTC

Cuts at:   3228    4572    3228 
   Size:       1344    4178
 
EaeI y'GGCC_r

Cuts at:   1248    1905    2039    3107    3281    3672    3699    5044    1248 
   Size:        657     134    1068     174     391      27    1345    1726

  Fragments arranged by size: 

                1726    1345    1068     657     391     174     134      27
            
 
EagI C'GGCC_G

Cuts at:   1905    3107    1905 
   Size:       1202    4320
 
EarI CTCTTCn'nnn_

Cuts at:    334     797    3545    3755     334 
   Size:        463    2748     210    2101

  Fragments arranged by size: 

                2748    2101     463     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3730    4472    4618    5332    3730 
   Size:        742     146     714    3920

  Fragments arranged by size: 

                3920     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    903    2534     903 
   Size:       1631    3891
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    315     491     631    1045    1521    1533    1774    1990    2029 
   Size:        176     140     414     476      12     241     216      39

Cuts at:   2029    2391    2445    3347    3779    4132    4794     315 
   Size:        362      54     902     432     353     662    1043

  Fragments arranged by size: 

                1043     902     662     476     432     414     362     353
                 241     216     176     140      54      39      12
 
EcoO109I rG'GnC_Cy

Cuts at:    663     664    1440    1588    2661     663 
   Size:          1     776     148    1073    3524

  Fragments arranged by size: 

                3524    1073     776     148       1
 
EcoRI G'AATT_C

Cuts at:    385    2526     385 
   Size:       2141    3381
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1278    1440    3541    1278 
   Size:        162    2101    3259

  Fragments arranged by size: 

                3259    2101     162
 
FspI TGC'GCA

Cuts at:   3303    3303 
   Size:       5522
 
HaeII r_GCGC'y

Cuts at:    134    1544    2956    3204    4434    5205     134 
   Size:       1410    1412     248    1230     771     451

  Fragments arranged by size: 

                1412    1410    1230     771     451     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    563     595    1445    1477    2268    2300    3364    3394    3396 
   Size:         32     850      32     791      32    1064      30       2

Cuts at:   3396    3426     563 
   Size:         30    2659

  Fragments arranged by size: 

                2659    1064     850     791      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2561    2561 
   Size:       5522
 
HindIII A'AGCT_T

Cuts at:    643     643 
   Size:       5522
 
KasI G'GCGC_C

Cuts at:   2952    3200    2952 
   Size:        248    5274
 
KpnI G_GTAC'C

Cuts at:   2542    2542 
   Size:       5522
 
MfeI C'AATT_G

Cuts at:    212     212 
   Size:       5522
 
MluI A'CGCG_T

Cuts at:   4955    4955 
   Size:       5522
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4281    4465    4281 
   Size:        184    5338
 
MscI TGG'CCA

Cuts at:   3283    3283 
   Size:       5522
 
MslI CAynn'nnrTG

Cuts at:    742     834    1072    1075    1190    1312    3511    3638     742 
   Size:         92     238       3     115     122    2199     127    2626

  Fragments arranged by size: 

                2626    2199     238     127     122     115      92       3
            
 
MspA1I CmG'CkG

Cuts at:    280     681    1916    2633    2947    3307    4091    4336     280 
   Size:        401    1235     717     314     360     784     245    1466

  Fragments arranged by size: 

                1466    1235     784     717     401     360     314     245
            
 
NaeI GCC'GGC

Cuts at:   3703    3703 
   Size:       5522
 
NarI GG'CG_CC

Cuts at:   2953    3201    2953 
   Size:        248    5274
 
NcoI C'CATG_G

Cuts at:   1073    2199    1073 
   Size:       1126    4396
 
NgoMIV G'CCGG_C

Cuts at:   3701    3701 
   Size:       5522
 
NspI r_CATG'y

Cuts at:    724    1666    2575    3075    4678     724 
   Size:        942     909     500    1603    1568

  Fragments arranged by size: 

                1603    1568     942     909     500
 
PasI CC'CwG_GG

Cuts at:   1281    1281 
   Size:       5522
 
PciI A'CATG_T

Cuts at:    720    1662    4674     720 
   Size:        942    3012    1568

  Fragments arranged by size: 

                3012    1568     942
 
PflMI CCAn_nnn'nTGG

Cuts at:   1023    1560    1023 
   Size:        537    4985
 
PfoI T'CCnGG_A

Cuts at:   2468    2468 
   Size:       5522
 
PmeI GTTT'AAAC

Cuts at:   2521    2521 
   Size:       5522
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3968    4000    3968 
   Size:         32    5490
 
PpuMI rG'GwC_Cy

Cuts at:   1440    1588    1440 
   Size:        148    5374
 
PspOMI G'GGCC_C

Cuts at:    663     663 
   Size:       5522
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1516    1548    1516 
   Size:         32    5490
 
PstI C_TGCA'G

Cuts at:   2569    3254    2569 
   Size:        685    4837
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5522
 
PvuII CAG'CTG

Cuts at:    681    2947    3307     681 
   Size:       2266     360    2896

  Fragments arranged by size: 

                2896    2266     360
 
RsrII CG'GwC_CG

Cuts at:   3717    3717 
   Size:       5522
 
SacI G_AGCT'C

Cuts at:    905    2536     905 
   Size:       1631    3891
 
SalI G'TCGA_C

Cuts at:   2559    2559 
   Size:       5522
 
SapI GCTCTTCn'nnn_

Cuts at:   3545    3755    3545 
   Size:        210    5312
 
SbfI CC_TGCA'GG

Cuts at:   2569    2569 
   Size:       5522
 
ScaI AGT'ACT

Cuts at:   1063    5039    1063 
   Size:       3976    1546
 
SexAI A'CCwGG_T

Cuts at:   1514    1514 
   Size:       5522
 
SfcI C'TryA_G

Cuts at:     33     317     393    1464    2565    3250    4218    4409      33 
   Size:        284      76    1071    1101     685     968     191    1146

  Fragments arranged by size: 

                1146    1101    1071     968     685     284     191      76
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    671     671 
   Size:       5522
 
SfoI GGC'GCC

Cuts at:   2954    3202    2954 
   Size:        248    5274
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5522
 
SmaI CCC'GGG

Cuts at:   1817    2544    1817 
   Size:        727    4795
 
SmlI C'TyrA_G

Cuts at:   1576    2677    3592    4306    4568    1576 
   Size:       1101     915     714     262    2530

  Fragments arranged by size: 

                2530    1101     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5522
 
SphI G_CATG'C

Cuts at:   2575    3075    2575 
   Size:        500    5022
 
StuI AGG'CCT

Cuts at:   1204    1204 
   Size:       5522
 
StyI C'CwwG_G

Cuts at:    119     956    1073    1097    1241    1923    2199    2231    2656 
   Size:        837     117      24     144     682     276      32     425

Cuts at:   2656    3010     119 
   Size:        354    2631

  Fragments arranged by size: 

                2631     837     682     425     354     276     144     117
                  32      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    249    1030    1700    1795    3105    3238    3697    3906     249 
   Size:        781     670      95    1310     133     459     209    1865

  Fragments arranged by size: 

                1865    1310     781     670     459     209     133      95
            
 
TatI w'GTAC_w

Cuts at:   1061    2018    5037    1061 
   Size:        957    3019    1546

  Fragments arranged by size: 

                3019    1546     957
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5039    5071    5039 
   Size:         32    5490
 
Tth111I GACn'n_nGTC

Cuts at:   3319    3319 
   Size:       5522
 
XbaI T'CTAG_A

Cuts at:   2553    2553 
   Size:       5522
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1020    3017    1020 
   Size:       1997    3525
 
XmaI C'CCGG_G

Cuts at:   1815    2542    1815 
   Size:        727    4795
 
XmnI GAAnn'nnTTC

Cuts at:   2303    2303 
   Size:       5522
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AlfI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BmtI    BsiWI    BsrGI   BssHII   BstEII   Bsu36I     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI     HpaI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI    PspXI
    SacII    SanDI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com