Restriction map for pF1KB3100
 (Linear) (Six-base) MAPSORT of: KIBB3100.seq  Check: 7501  from: 1  to: 2568



KIBB3100.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2352    2568 
   Size:       2352     216
 
AccI GT'mk_AC

Cuts at:      0     820    2568 
   Size:        820    1748
 
Acc65I G'GTAC_C

Cuts at:      0    1360    2568 
   Size:       1360    1208
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     488     617    2568 
   Size:         37     451     129    1951

  Fragments arranged by size: 

                1951     451     129      37
 
AfeI AGC'GCT

Cuts at:      0    1822    1882    2568 
   Size:       1822      60     686

  Fragments arranged by size: 

                1822     686      60
 
AlwNI CAG_nnn'CTG

Cuts at:      0     829    2568 
   Size:        829    1739
 
AvaI C'yCGr_G

Cuts at:      0      65     169     241     276     674    1182    2568 
   Size:         65     104      72      35     398     508    1386

  Fragments arranged by size: 

                1386     508     398     104      72      65      35
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     326     359     614     647    2568 
   Size:        326      33     255      33    1921

  Fragments arranged by size: 

                1921     326     255      33      33
 
BanI G'GyrC_C

Cuts at:      0    1231    1360    2568 
   Size:       1231     129    1208

  Fragments arranged by size: 

                1231    1208     129
 
BanII G_rGCy'C

Cuts at:      0     143     208     266     738    1166    2073    2568 
   Size:        143      65      58     472     428     907     495

  Fragments arranged by size: 

                 907     495     472     428     143      65      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     430    2568 
   Size:        430    2138
 
BbvCI CC'TCA_GC

Cuts at:      0     584    2426    2568 
   Size:        584    1842     142

  Fragments arranged by size: 

                1842     584     142
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     391     425    2568 
   Size:        391      34    2143

  Fragments arranged by size: 

                2143     391      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1234    2568 
   Size:       1234    1334
 
BglI GCCn_nnn'nGGC

Cuts at:      0     243     492    2568 
   Size:        243     249    2076

  Fragments arranged by size: 

                2076     249     243
 
BlpI GC'TnA_GC

Cuts at:      0      89    2568 
   Size:         89    2479
 
Bme1580I G_kGCm'C

Cuts at:      0     176    1234    2568 
   Size:        176    1058    1334

  Fragments arranged by size: 

                1334    1058     176
 
BmgBI CAC'GTC

Cuts at:      0    1020    2568 
   Size:       1020    1548
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     929    1449    2568 
   Size:        929     520    1119

  Fragments arranged by size: 

                1119     929     520
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     222     816     915    2568 
   Size:        222     594      99    1653

  Fragments arranged by size: 

                1653     594     222      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235    1925    2568 
   Size:        235    1690     643

  Fragments arranged by size: 

                1690     643     235
 
Bpu10I CC'TnA_GC

Cuts at:      0     584    2426    2568 
   Size:        584    1842     142

  Fragments arranged by size: 

                1842     584     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     466    2196    2568 
   Size:         58     408    1730     372

  Fragments arranged by size: 

                1730     408     372      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187     372    1548    2568 
   Size:        187     185    1176    1020

  Fragments arranged by size: 

                1176    1020     187     185
 
BsaAI yAC'GTr

Cuts at:      0     840    2568 
   Size:        840    1728
 
BsaBI GATnn'nnATC

Cuts at:      0     784    2568 
   Size:        784    1784
 
BsaHI Gr'CG_yC

Cuts at:      0     146    2568 
   Size:        146    2422
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2026    2056    2568 
   Size:       2026      30     512

  Fragments arranged by size: 

                2026     512      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     133     199     390     572    1967    1982    2568 
   Size:         52      81      66     191     182    1395      15     586

  Fragments arranged by size: 

                1395     586     191     182      81      66      52      15
            
 
BseYI C'CCAG_C

Cuts at:      0     878    1427    1801    2012    2075    2123    2143    2431 
   Size:        878     549     374     211      63      48      20     288

Cuts at:   2431    2459    2568 
   Size:         28     109

  Fragments arranged by size: 

                 878     549     374     288     211     109      63      48
                  28      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     447     557    2568 
   Size:        143     304     110    2011

  Fragments arranged by size: 

                2011     304     143     110
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1536    2568 
   Size:       1536    1032
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     176     208     266     447     557     738    1166 
   Size:        143      33      32      58     181     110     181     428

Cuts at:   1166    1234    2073    2568 
   Size:         68     839     495

  Fragments arranged by size: 

                 839     495     428     181     181     143     110      68
                  58      33      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2352    2568 
   Size:       2352     216
 
BsrBI CCG'CTC

Cuts at:      0      93     135     578     699    2568 
   Size:         93      42     443     121    1869

  Fragments arranged by size: 

                1869     443     121      93      42
 
BsrDI GCAATG_nn'

Cuts at:      0    1058    2568 
   Size:       1058    1510
 
BsrFI r'CCGG_y

Cuts at:      0     941    1403    2568 
   Size:        941     462    1165

  Fragments arranged by size: 

                1165     941     462
 
BtgI C'CryG_G

Cuts at:      0      51     299    1857    1914    2568 
   Size:         51     248    1558      57     654

  Fragments arranged by size: 

                1558     654     248      57      51
 
BtsI GCAGTG_nn'

Cuts at:      0    1139    2470    2568 
   Size:       1139    1331      98

  Fragments arranged by size: 

                1331    1139      98
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1818    1853    2568 
   Size:       1818      35     715

  Fragments arranged by size: 

                1818     715      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1457    2551    2568 
   Size:       1457    1094      17

  Fragments arranged by size: 

                1457    1094      17
 
EaeI y'GGCC_r

Cuts at:      0    1422    2497    2568 
   Size:       1422    1075      71

  Fragments arranged by size: 

                1422    1075      71
 
EarI CTCTTCn'nnn_

Cuts at:      0     593     884    2568 
   Size:        593     291    1684

  Fragments arranged by size: 

                1684     593     291
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2093    2568 
   Size:       2093     475
 
EcoICRI GAG'CTC

Cuts at:      0     141    2568 
   Size:        141    2427
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     235     488     617    1925    2568 
   Size:         37     198     253     129    1308     643

  Fragments arranged by size: 

                1308     643     253     198     129      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1723    2568 
   Size:       1723     845
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1098    1608    1897    2568 
   Size:       1098     510     289     671

  Fragments arranged by size: 

                1098     671     510     289
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1101    2568 
   Size:       1101    1467
 
FspI TGC'GCA

Cuts at:      0    1131    2568 
   Size:       1131    1437
 
HaeII r_GCGC'y

Cuts at:      0      51    1824    1884    2219    2568 
   Size:         51    1773      60     335     349

  Fragments arranged by size: 

                1773     349     335      60      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2409    2441    2568 
   Size:       2409      32     127

  Fragments arranged by size: 

                2409     127      32
 
HindIII A'AGCT_T

Cuts at:      0     470    2568 
   Size:        470    2098
 
KpnI G_GTAC'C

Cuts at:      0    1364    2568 
   Size:       1364    1204
 
MfeI C'AATT_G

Cuts at:      0     704    2568 
   Size:        704    1864
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1218    1355    2069    2568 
   Size:         89    1129     137     714     499

  Fragments arranged by size: 

                1129     714     499     137      89
 
MslI CAynn'nnrTG

Cuts at:      0     723    2568 
   Size:        723    1845
 
MspA1I CmG'CkG

Cuts at:      0      53    1410    1427    1749    1859    1946    1985    2012 
   Size:         53    1357      17     322     110      87      39      27

Cuts at:   2012    2568 
   Size:        556

  Fragments arranged by size: 

                1357     556     322     110      87      53      39      27
                  17
 
NaeI GCC'GGC

Cuts at:      0    1405    2568 
   Size:       1405    1163
 
NcoI C'CATG_G

Cuts at:      0     299    2568 
   Size:        299    2269
 
NgoMIV G'CCGG_C

Cuts at:      0    1403    2568 
   Size:       1403    1165
 
NruI TCG'CGA

Cuts at:      0      35    2568 
   Size:         35    2533
 
NspI r_CATG'y

Cuts at:      0    1324    2568 
   Size:       1324    1244
 
PasI CC'CwG_GG

Cuts at:      0    2170    2568 
   Size:       2170     398
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1708    2568 
   Size:       1708     860
 
PfoI T'CCnGG_A

Cuts at:      0    1718    2536    2568 
   Size:       1718     818      32

  Fragments arranged by size: 

                1718     818      32
 
PmlI CAC'GTG

Cuts at:      0     840    2568 
   Size:        840    1728
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1717    1749    2568 
   Size:       1717      32     819

  Fragments arranged by size: 

                1717     819      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1608    1897    2568 
   Size:       1608     289     671

  Fragments arranged by size: 

                1608     671     289
 
PspXI vC'TCGA_Gb

Cuts at:      0      65    2568 
   Size:         65    2503
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     313     345    2506    2538    2568 
   Size:        313      32    2161      32      30

  Fragments arranged by size: 

                2161     313      32      32      30
 
PstI C_TGCA'G

Cuts at:      0      47     101     251     874    1748    2568 
   Size:         47      54     150     623     874     820

  Fragments arranged by size: 

                 874     820     623     150      54      47
 
SacI G_AGCT'C

Cuts at:      0     143    2568 
   Size:        143    2425
 
SacII CC_GC'GG

Cuts at:      0      54    1860    2568 
   Size:         54    1806     708

  Fragments arranged by size: 

                1806     708      54
 
SapI GCTCTTCn'nnn_

Cuts at:      0     884    2568 
   Size:        884    1684
 
ScaI AGT'ACT

Cuts at:      0    2476    2568 
   Size:       2476      92
 
SfcI C'TryA_G

Cuts at:      0      43      97     247     821     870    1744    1998    2148 
   Size:         43      54     150     574      49     874     254     150

Cuts at:   2148    2247    2568 
   Size:         99     321

  Fragments arranged by size: 

                 874     574     321     254     150     150      99      54
                  49      43
 
SmaI CCC'GGG

Cuts at:      0     171     243    1184    2568 
   Size:        171      72     941    1384

  Fragments arranged by size: 

                1384     941     171      72
 
SmlI C'TyrA_G

Cuts at:      0      65      73     481    2211    2568 
   Size:         65       8     408    1730     357

  Fragments arranged by size: 

                1730     408     357      65       8
 
SrfI GCCC'GGGC

Cuts at:      0     243    2568 
   Size:        243    2325
 
SspI AAT'ATT

Cuts at:      0     309    2568 
   Size:        309    2259
 
StuI AGG'CCT

Cuts at:      0      71     406     519    2568 
   Size:         71     335     113    2049

  Fragments arranged by size: 

                2049     335     113      71
 
StyI C'CwwG_G

Cuts at:      0     299    1085    2568 
   Size:        299     786    1483

  Fragments arranged by size: 

                1483     786     299
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     211     462     892    1468    1634    2157    2568 
   Size:        211     251     430     576     166     523     411

  Fragments arranged by size: 

                 576     523     430     411     251     211     166
 
TatI w'GTAC_w

Cuts at:      0    1346    1778    2474    2560    2568 
   Size:       1346     432     696      86       8

  Fragments arranged by size: 

                1346     696     432      86       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1004    1036    2568 
   Size:       1004      32    1532

  Fragments arranged by size: 

                1532    1004      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1371    2568 
   Size:       1371    1197
 
XhoI C'TCGA_G

Cuts at:      0      65    2568 
   Size:         65    2503
 
XmaI C'CCGG_G

Cuts at:      0     169     241    1182    2568 
   Size:        169      72     941    1386

  Fragments arranged by size: 

                1386     941     169      72
 
XmnI GAAnn'nnTTC

Cuts at:      0     325    2568 
   Size:        325    2243
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AlwNI     AvaI     BaeI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI     BtgI     BtsI    CspCI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FspI    HaeII    Hin4I  HindIII     KpnI     MfeI     MmeI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NruI     NspI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PspXI     PsrI     PstI
     SacI    SacII     SapI     ScaI     SfcI     SmaI     SmlI     SrfI
     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI     BclI    BfrBI    BglII     BmtI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI
   HincII     HpaI     KasI     MluI     MscI     NarI     NdeI     NheI
     NotI     NsiI     PacI     PciI     PmeI    PshAI     PsiI   PspOMI
     PvuI    PvuII    RsrII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SwaI  Tth111I
     XbaI     ZraI
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