Restriction map for pF1KB3098
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AclI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BcgI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrGI       BstBI       BstEII      BstPI       BstXI       
CelII       ClaI        CpoI        DraIII      Eam1105I    
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NcoI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tsp45I      
Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          882  GTATAC               GT^[AC][GT]AC
AccIII        717  TCCGGA               T^CCGGA
AcyI          289  GGCGCC               G[GA]^CG[CT]C
AocI          740  CCTCAGG              CC^T[ACGT]AGG
ApaI          214  GGGCCC               GGGCC^C
AscI          465  GGCGCGCC             GG^CGCGCC
AseI          703  ATTAAT               AT^TAAT
AsnI          703  ATTAAT               AT^TAAT
AspHI        1832  GAGCAC               G[AT]GC[AT]^C
AspI          746  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1853  TGGCCA               TGG^CCA
BbeI          289  GGCGCC               GGCGC^C
BbvCI        1822  GCTGAGG              CCTCAGC(-5/-2)
BclI         1107  TGATCA               T^GATCA
BsaHI         289  GGCGCC               G[GA]^CG[CT]C
BseAI         717  TCCGGA               T^CCGGA
BsiEI         499  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1832  GAGCAC               G[AT]GC[AT]^C
BsmAI        1340  GAGAC                GTCTC(1/5)
BsmBI        1340  GAGACG               CGTCTC(1/5)
BsmFI         876  GTCCC                GGGAC(10/14)
BspEI         717  TCCGGA               T^CCGGA
BspMI         837  ACCTGC               ACCTGC(4/8)
BsrDI        1678  GCAATG               GCAATG(2/0)
BssHII        466  GCGCGC               G^CGCGC
BssSI         336  CTCGTG               CACGAG(-5/-1)
Bst1107I      882  GTATAC               GTA^TAC
BstAPI       1810  GCAAAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       882  GTATAC               GTA^TAC
Bsu36I        740  CCTCAGG              CC^T[ACGT]AGG
DraII         272  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          746  GACAGTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          499  CGGCCG               C^GGCCG
EclXI         499  CGGCCG               C^GGCCG
Eco52I        499  CGGCCG               C^GGCCG
Eco81I        740  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      272  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       966  ATGCAT               ATGCA^T
FbaI         1107  TGATCA               T^GATCA
FseI          239  GGCCGGCC             GGCCGG^CC
HgaI          445  GCGTC                GACGC(5/10)
Hin1I         289  GGCGCC               G[GA]^CG[CT]C
KasI          289  GGCGCC               G^GCGCC
MaeII         769  ACGT                 A^CGT
MluNI        1853  TGGCCA               TGG^CCA
MroI          717  TCCGGA               T^CCGGA
MscI         1853  TGGCCA               TGG^CCA
MslI         1066  CATTCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          289  GGCGCC               GG^CGCC
NdeI         1111  CATATG               CA^TATG
NsiI          966  ATGCAT               ATGCA^T
NspI         1395  GCATGT               [GA]CATG^[CT]
PflFI         746  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         272  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         703  ATTAAT               AT^TAAT
PvuII        1820  CAGCTG               CAG^CTG
SfoI          289  GGCGCC               GGC^GCC
Tth111I       746  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1717  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI          67  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
               90  CAGCGACTG           
BanI          289  GGCGCC               G^G[CT][GA]CC
              514  GGCACC              
BbsI          793  GAAGAC               GAAGAC(2/6)
             1667  GAAGAC              
BciVI         645  GGATAC               GTATCC(6/5)
             1598  GGATAC              
BglI           43  GCCGGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              470  GCCGGGCGGGC         
BpuAI         793  GAAGAC               GAAGAC(2/6)
             1667  GAAGAC              
BsaWI         717  TCCGGA               [AT]^CCGG[AT]
             1847  ACCGGA              
BsgI           54  CTGCAC               GTGCAG(16/14)
             1727  GTGCAG              
BspHI         897  TCATGA               T^CATGA
             1181  TCATGA              
BstNI         399  CCTGG                CC^[AT]GG
              436  CCAGG               
DraI         1192  TTTAAA               TTT^AAA
             1375  TTTAAA              
EcoRII        399  CCTGG                ^CC[AT]GG
              436  CCAGG               
HaeII         221  AGCGCC               [GA]GCGC^[CT]
              289  GGCGCC              
HphI          790  GGTGA                GGTGA(8/7)
             1384  GGTGA               
KspI          451  CCGCGG               CCGC^GG
              496  CCGCGG              
MaeIII       1187  GTTAC                ^GT[ACGT]AC
             1399  GTAAC               
MvaI          399  CCTGG                CC^[AT]GG
              436  CCAGG               
NaeI          240  GCCGGC               GCC^GGC
              381  GCCGGC              
NgoMIV        240  GCCGGC               G^CCGGC
              381  GCCGGC              
PstI           87  CTGCAG               CTGCA^G
             1817  CTGCAG              
RcaI          897  TCATGA               T^CATGA
             1181  TCATGA              
SacII         451  CCGCGG               CCGC^GG
              496  CCGCGG              
SgrAI         380  CGCCGGCG             C[GA]^CCGG[CT]G
              416  CGCCGGTG            


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
ApoI        Asp700I     AvaI        AvaII       BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmI        
BsoBI       Bsp1286I    BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        Ksp632I     MaeI        
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SspI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
XmaCI       XmaI        XmnI        
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