Restriction map for pF1KB3090
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MslI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Van91I      
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          150  GTCTAC               GT^[AC][GT]AC
AcsI          753  AAATTC               [GA]^AATT[CT]
AflIII       1417  ACATGT               A^C[GA][CT]GT
ApoI          753  AAATTC               [GA]^AATT[CT]
AspI          235  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         364  GGATCC               G^GATCC
BanI          177  GGTGCC               G^G[CT][GA]CC
BbsI          499  GAAGAC               GAAGAC(2/6)
BcnI          173  CCGGG                CC^[GC]GG
BmyI         1784  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         499  GAAGAC               GAAGAC(2/6)
BsaI         1217  GAGACC               GGTCTC(1/5)
BsaWI         719  ACCGGA               [AT]^CCGG[AT]
BsmBI         383  CGTCTC               CGTCTC(1/5)
BsmFI          35  GTCCC                GGGAC(10/14)
Bsp1286I     1784  GTGCCC               G[AGT]GC[ACT]^C
BspHI        1166  TCATGA               T^CATGA
BspLU11I     1417  ACATGT               A^CATGT
BssSI         203  CTCGTG               CACGAG(-5/-1)
BstF5I        699  GGATG                GGATG(2/0)
BstYI         364  GGATCC               [GA]^GATC[CT]
DraI         1446  TTTAAA               TTT^AAA
DsaI          964  CCATGG               C^C[GA][CT]GG
EcoRV        1052  GATATC               GAT^ATC
FauI          169  CCCGC                CCCGC(4/6)
FokI          699  GGATG                GGATG(9/13)
HincII       1080  GTCAAC               GT[CT]^[GA]AC
HindII       1080  GTCAAC               GT[CT]^[GA]AC
MaeII         382  ACGT                 A^CGT
MflI          364  GGATCC               [GA]^GATC[CT]
MspA1I       1072  CAGCTG               C[AC]G^C[GT]G
NciI          173  CCGGG                CC^[GC]GG
NcoI          964  CCATGG               C^CATGG
NdeI          574  CATATG               CA^TATG
PflFI         235  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        1072  CAGCTG               CAG^CTG
RcaI         1166  TCATGA               T^CATGA
ScaI          544  AGTACT               AGT^ACT
SexAI        1220  ACCTGGT              A^CC[AT]GGT
SmlI          936  CTTGAG               C^T[CT][GA]AG
SphI          794  GCATGC               GCATG^C
Tth111I       235  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         364  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          170  CCGC                 CCGC(-3/-1)
             1558  GCGG                
AlwNI         340  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              681  CAGTAACTG           
AseI         1377  ATTAAT               AT^TAAT
             1462  ATTAAT              
AsnI         1377  ATTAAT               AT^TAAT
             1462  ATTAAT              
BalI          580  TGGCCA               TGG^CCA
              835  TGGCCA              
BseRI        1330  GAGGAG               GAGGAG(10/8)
             1686  GAGGAG              
BsrDI         538  GCAATG               GCAATG(2/0)
              899  GCAATG              
DraII          33  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              636  AGGACCT             
EarI          507  CTCTTC               CTCTTC(1/4)
             1327  GAAGAG              
EcoO109I       33  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              636  AGGACCT             
HapII         173  CCGG                 C^CGG
              720  CCGG                
HpaII         173  CCGG                 C^CGG
              720  CCGG                
HphI         1224  GGTGA                GGTGA(8/7)
              598  TCACC               
Ksp632I       507  CTCTTC               CTCTTC(1/4)
             1327  GAAGAG              
MfeI         1411  CAATTG               C^AATTG
             1681  CAATTG              
MluNI         580  TGGCCA               TGG^CCA
              835  TGGCCA              
MscI          580  TGGCCA               TGG^CCA
              835  TGGCCA              
MspI          173  CCGG                 C^CGG
              720  CCGG                
MunI         1411  CAATTG               C^AATTG
             1681  CAATTG              
PleI         1594  GACTC                GAGTC(4/5)
              195  GAGTC               
PpuMI          33  AGGTCCC              [GA]G^G[AT]CC[CT]
              636  AGGACCT             
PshBI        1377  ATTAAT               AT^TAAT
             1462  ATTAAT              
TaqI          514  TCGA                 T^CGA
              813  TCGA                
Tsp45I        597  GTCAC                ^GT[GC]AC
             1668  GTCAC               
TthHB8I       514  TCGA                 T^CGA
              813  TCGA                
XbaI          487  TCTAGA               T^CTAGA
              759  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AfaI        AluI        AlwI        AvaII       BbvI        
BfaI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      HinfI       ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       
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