Restriction map for pF1KB3088
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BbrPI       BbsI        
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspHI       BspLU11I    BsrBI       
BsrFI       BssSI       BstBI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HpaI        KpnI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MscI        MslI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          886  GTATAC               GT^[AC][GT]AC
AccIII        477  TCCGGA               T^CCGGA
Asp700I      1144  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         725  GTGCTC               G[AT]GC[AT]^C
AvaI         1079  CTCGAG               C^[CT]CG[GA]G
BamHI         474  GGATCC               G^GATCC
BanII         235  GAGCCC               G[GA]GC[CT]^C
BbvCI          60  GCTGAGG              CCTCAGC(-5/-2)
BglI          155  GCCATGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         477  TCCGGA               [AT]^CCGG[AT]
BseAI         477  TCCGGA               T^CCGGA
BsiHKAI       725  GTGCTC               G[AT]GC[AT]^C
BsmAI         489  GTCTC                GTCTC(1/5)
BsoBI        1079  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      680  TGTACA               T^GTACA
BspEI         477  TCCGGA               T^CCGGA
BspMI          80  ACCTGC               ACCTGC(4/8)
BsrGI         680  TGTACA               T^GTACA
BssHII        145  GCGCGC               G^CGCGC
Bst1107I      886  GTATAC               GTA^TAC
BstAPI       1084  GCAGCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        747  GGTAACC              G^GT[ACGT]ACC
BstPI         747  GGTAACC              G^GT[ACGT]ACC
BstXI        1095  CCACGCCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       886  GTATAC               GTA^TAC
EaeI          153  CGGCCA               [CT]^GGCC[GA]
EarI         1000  GAAGAG               CTCTTC(1/4)
EcoNI         114  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       747  GGTAACC              G^GT[ACGT]ACC
HgaI          289  GACGC                GACGC(5/10)
HincII        220  GTCAAC               GT[CT]^[GA]AC
HindII        220  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1000  GAAGAG               CTCTTC(1/4)
MroI          477  TCCGGA               T^CCGGA
NspI          537  GCATGT               [GA]CATG^[CT]
PaeR7I       1079  CTCGAG               C^TCGAG
ScaI         1043  AGTACT               AGT^ACT
SspBI         680  TGTACA               T^GTACA
XhoI         1079  CTCGAG               C^TCGAG
XmnI         1144  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          184  GGCGCC               G[GA]^CG[CT]C
              279  GGCGCC              
AfaI          681  GTAC                 GT^AC
             1044  GTAC                
AlwNI         165  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1085  CAGCTTCTG           
BbeI          184  GGCGCC               GGCGC^C
              279  GGCGCC              
BclI          417  TGATCA               T^GATCA
              546  TGATCA              
BcnI           15  CCCGG                CC^[GC]GG
              194  CCGGG               
BmyI          235  GAGCCC               G[AGT]GC[ACT]^C
              725  GTGCTC              
BsaHI         184  GGCGCC               G[GA]^CG[CT]C
              279  GGCGCC              
BsgI          347  GTGCAG               GTGCAG(16/14)
             1010  GTGCAG              
Bsp1286I      235  GAGCCC               G[AGT]GC[ACT]^C
              725  GTGCTC              
BsrDI         909  CATTGC               GCAATG(2/0)
             1123  GCAATG              
BsrI          669  ACTGG                ACTGG(1/-1)
              310  CCAGT               
BstYI          11  AGATCC               [GA]^GATC[CT]
              474  GGATCC              
DsaI           74  CCGCGG               C^C[GA][CT]GG
              284  CCGCGG              
FbaI          417  TGATCA               T^GATCA
              546  TGATCA              
HaeII         184  GGCGCC               [GA]GCGC^[CT]
              279  GGCGCC              
HaeIII        154  GGCC                 GG^CC
              672  GGCC                
Hin1I         184  GGCGCC               G[GA]^CG[CT]C
              279  GGCGCC              
HindIII       353  AAGCTT               A^AGCTT
              804  AAGCTT              
KasI          184  GGCGCC               G^GCGCC
              279  GGCGCC              
KspI           74  CCGCGG               CCGC^GG
              284  CCGCGG              
MflI           11  AGATCC               [GA]^GATC[CT]
              474  GGATCC              
NarI          184  GGCGCC               GG^CGCC
              279  GGCGCC              
NciI           15  CCCGG                CC^[GC]GG
              194  CCGGG               
RsaI          681  GTAC                 GT^AC
             1044  GTAC                
SacII          74  CCGCGG               CCGC^GG
              284  CCGCGG              
SfoI          184  GGCGCC               GGC^GCC
              279  GGCGCC              
SmlI          491  CTCAAG               C^T[CT][GA]AG
             1079  CTCGAG              
XhoII          11  AGATCC               [GA]^GATC[CT]
              474  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BanI        BbvI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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