Restriction map for pF1KB3088
 (Circular) (Six-base) MAPSORT of: pF1KB3088.seq  Check: 6895  from: 1  to: 4367



pF1KB3088  4367 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AccI GT'mk_AC

Cuts at:    995    1405     995 
   Size:        410    3957
 
Acc65I G'GTAC_C

Cuts at:   1383    1383 
   Size:       4367
 
AclI AA'CG_TT

Cuts at:   4207    4207 
   Size:       4367
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    846    2192    2624    2977    3639     846 
   Size:       1346     432     353     662    1574

  Fragments arranged by size: 

                1574    1346     662     432     353
 
AflIII A'CryG_T

Cuts at:   1719    3519    3800    1719 
   Size:       1800     281    2286

  Fragments arranged by size: 

                2286    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    278    1198    1672    3110     278 
   Size:        920     474    1438    1535

  Fragments arranged by size: 

                1535    1438     920     474
 
ApaLI G'TGCA_C

Cuts at:   3205    3205 
   Size:       4367
 
ApoI r'AATT_y

Cuts at:   1371    3720    3976    1371 
   Size:       2349     256    1762

  Fragments arranged by size: 

                2349    1762     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4367
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4367
 
AvaI C'yCGr_G

Cuts at:   1187    1387    1187 
   Size:        200    4167
 
BamHI G'GATC_C

Cuts at:    582    1392     582 
   Size:        810    3557
 
BanI G'GyrC_C

Cuts at:    173     247     292     310     387     985    1383    1797    2045 
   Size:         74      45      18      77     598     398     414     248

Cuts at:   2045    2080     173 
   Size:         35    2460

  Fragments arranged by size: 

                2460     598     414     398     248      77      74      45
                  35      18
 
BanII G_rGCy'C

Cuts at:    347    1381    2411     347 
   Size:       1034    1030    2303

  Fragments arranged by size: 

                2303    1034    1030
 
BbeI G_GCGC'C

Cuts at:    296     391    1801    2049     296 
   Size:         95    1410     248    2614

  Fragments arranged by size: 

                2614    1410     248      95
 
BbvCI CC'TCA_GC

Cuts at:    169     169 
   Size:       4367
 
BciVI GTATCCnnnnn_n'

Cuts at:   2265    3321    4367    2265 
   Size:       1056    1046    2265

  Fragments arranged by size: 

                2265    1056    1046
 
BclI T'GATC_A

Cuts at:    525     654    1887     525 
   Size:        129    1233    3005

  Fragments arranged by size: 

                3005    1233     129
 
BglI GCCn_nnn'nGGC

Cuts at:    269     269 
   Size:       4367
 
BglII A'GATC_T

Cuts at:   1882    3919    1882 
   Size:       2037    2330
 
BlpI GC'TnA_GC

Cuts at:   1479    1479 
   Size:       4367
 
Bme1580I G_kGCm'C

Cuts at:   1992    2085    3209    1992 
   Size:         93    1124    3150

  Fragments arranged by size: 

                3150    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1579    1660    1995    4139    1579 
   Size:         81     335    2144    1807

  Fragments arranged by size: 

                2144    1807     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2734    2734 
   Size:       4367
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     401    1082     214 
   Size:        187     681    3499

  Fragments arranged by size: 

                3499     681     187
 
Bpu10I CC'TnA_GC

Cuts at:    169    1022     169 
   Size:        853    3514
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    584    1543    2422    3136    3434     584 
   Size:        959     879     714     298    1517

  Fragments arranged by size: 

                1517     959     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4071    4071 
   Size:       4367
 
BsaAI yAC'GTr

Cuts at:   1720    1720 
   Size:       4367
 
BsaBI GATnn'nnATC

Cuts at:      6    1881    3918       6 
   Size:       1875    2037     455

  Fragments arranged by size: 

                2037    1875     455
 
BsaHI Gr'CG_yC

Cuts at:    293     388    1798    2046    2748    4244     293 
   Size:         95    1410     248     702    1496     416

  Fragments arranged by size: 

                1496    1410     702     416     248      95
 
BsaWI w'CCGG_w

Cuts at:    585    1556    2077    2755    3166    3313    3587     585 
   Size:        971     521     678     411     147     274    1365

  Fragments arranged by size: 

                1365     971     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    580     610     580 
   Size:         30    4337
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    355     388     391     394     355 
   Size:         33       3       3    4328

  Fragments arranged by size: 

                4328      33       3       3
 
BseYI C'CCAG_C

Cuts at:    316    1792    2550    3215    3623     316 
   Size:       1476     758     665     408    1060

  Fragments arranged by size: 

                1476    1060     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    476    1139     476 
   Size:        663    3704
 
BsiEI CG_ry'CG

Cuts at:    104    1909    1955    3185     104 
   Size:       1805      46    1230    1286

  Fragments arranged by size: 

                1805    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    837    1381    2159    2349    3209     837 
   Size:        544     778     190     860    1995

  Fragments arranged by size: 

                1995     860     778     544     190
 
Bsp1286I G_dGCh'C

Cuts at:    347     837    1381    1992    2085    2159    2349    2411    3209 
   Size:        490     544     611      93      74     190      62     798

Cuts at:   3209     347 
   Size:       1505

  Fragments arranged by size: 

                1505     798     611     544     490     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    585     585 
   Size:       4367
 
BspHI T'CATG_A

Cuts at:   2799    2799 
   Size:       4367
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    197    1417    1631    1933    2314     197 
   Size:       1220     214     302     381    2250

  Fragments arranged by size: 

                2250    1220     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2659    2713    4197    4362      50 
   Size:       2609      54    1484     165      55

  Fragments arranged by size: 

                2609    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1016    1238    2279    1016 
   Size:        222    1041    3104

  Fragments arranged by size: 

                3104    1041     222
 
BsrFI r'CCGG_y

Cuts at:   2365    2546    2365 
   Size:        181    4186
 
BsrGI T'GTAC_A

Cuts at:    788     788 
   Size:       4367
 
BssHII G'CGCG_C

Cuts at:    253     253 
   Size:       4367
 
BssSI C'ACGA_G

Cuts at:   2638    3346    2638 
   Size:        708    3659
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1198    1744    1198 
   Size:        546    3821
 
BstBI TT'CG_AA

Cuts at:   2728    2728 
   Size:       4367
 
BstEII G'GTnAC_C

Cuts at:    855     855 
   Size:       4367
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1210    1210 
   Size:       4367
 
BstYI r'GATC_y

Cuts at:      1     119     582    1392    1859    1882    2217    2463    2769 
   Size:        118     463     810     467      23     335     246     306

Cuts at:   2769    2781    2867    3919       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1052     810     467     463     449     335     306     246
                 118      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    996     996 
   Size:       4367
 
BtgI C'CryG_G

Cuts at:    182     392     182 
   Size:        210    4157
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1278    2317    2498    2661    1278 
   Size:       1039     181     163    2984

  Fragments arranged by size: 

                2984    1039     181     163
 
BtsI GCAGTG_nn'

Cuts at:    815     925    1355    1610     815 
   Size:        110     430     255    3572

  Fragments arranged by size: 

                3572     430     255     110
 
DraI TTT'AAA

Cuts at:   1366    1366 
   Size:       4367
 
DrdI GACnn_nn'nnGTC

Cuts at:   2073    3417    2073 
   Size:       1344    3023
 
EaeI y'GGCC_r

Cuts at:    261    1952    2126    2517    2544    3889     261 
   Size:       1691     174     391      27    1345     739

  Fragments arranged by size: 

                1691    1345     739     391     174      27
 
EagI C'GGCC_G

Cuts at:   1952    1952 
   Size:       4367
 
EarI CTCTTCn'nnn_

Cuts at:   1103    2390    2600    1103 
   Size:       1287     210    2870

  Fragments arranged by size: 

                2870    1287     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2575    3317    3463    4177    2575 
   Size:        742     146     714    2765

  Fragments arranged by size: 

                2765     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1379    1379 
   Size:       4367
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     401     846    1082    2192    2624    2977    3639     214 
   Size:        187     445     236    1110     432     353     662     942

  Fragments arranged by size: 

                1110     942     662     445     432     353     236     187
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    226     226 
   Size:       4367
 
EcoO109I rG'GnC_Cy

Cuts at:    275     285    1264    1506     275 
   Size:         10     979     242    3136

  Fragments arranged by size: 

                3136     979     242      10
 
EcoRI G'AATT_C

Cuts at:   1371    1371 
   Size:       4367
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2386    2386 
   Size:       4367
 
FspI TGC'GCA

Cuts at:   2148    2148 
   Size:       4367
 
HaeII r_GCGC'y

Cuts at:    296     391    1801    2049    3279    4050     296 
   Size:         95    1410     248    1230     771     613

  Fragments arranged by size: 

                1410    1230     771     613     248      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    481     495     513     527     580     612     998    1030    2209 
   Size:         14      18      14      53      32     386      32    1179

Cuts at:   2209    2239    2241    2271     481 
   Size:         30       2      30    2577

  Fragments arranged by size: 

                2577    1179     386      53      32      32      30      30
                  18      14      14       2
 
HincII GTy'rAC

Cuts at:    330    1406     330 
   Size:       1076    3291
 
HindIII A'AGCT_T

Cuts at:    461     912     461 
   Size:        451    3916
 
KasI G'GCGC_C

Cuts at:    292     387    1797    2045     292 
   Size:         95    1410     248    2614

  Fragments arranged by size: 

                2614    1410     248      95
 
KpnI G_GTAC'C

Cuts at:   1387    1387 
   Size:       4367
 
MluI A'CGCG_T

Cuts at:   3800    3800 
   Size:       4367
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    380     711    3126    3310     380 
   Size:        331    2415     184    1437

  Fragments arranged by size: 

                2415    1437     331     184
 
MscI TGG'CCA

Cuts at:   2128    2128 
   Size:       4367
 
MslI CAynn'nnrTG

Cuts at:   2356    2483    2356 
   Size:        127    4240
 
MspA1I CmG'CkG

Cuts at:    184     298     394    1478    1792    2152    2936    3181     184 
   Size:        114      96    1084     314     360     784     245    1370

  Fragments arranged by size: 

                1370    1084     784     360     314     245     114      96
            
 
NaeI GCC'GGC

Cuts at:   2548    2548 
   Size:       4367
 
NarI GG'CG_CC

Cuts at:    293     388    1798    2046     293 
   Size:         95    1410     248    2614

  Fragments arranged by size: 

                2614    1410     248      95
 
NgoMIV G'CCGG_C

Cuts at:   2546    2546 
   Size:       4367
 
NspI r_CATG'y

Cuts at:    649    1420    1920    3523     649 
   Size:        771     500    1603    1493

  Fragments arranged by size: 

                1603    1493     771     500
 
PciI A'CATG_T

Cuts at:   3519    3519 
   Size:       4367
 
PfoI T'CCnGG_A

Cuts at:    499     499 
   Size:       4367
 
PmeI GTTT'AAAC

Cuts at:   1366    1366 
   Size:       4367
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2813    2845    2813 
   Size:         32    4335
 
PpuMI rG'GwC_Cy

Cuts at:    275     285    1264     275 
   Size:         10     979    3378

  Fragments arranged by size: 

                3378     979      10
 
PsiI TTA'TAA

Cuts at:    467     467 
   Size:       4367
 
PspXI vC'TCGA_Gb

Cuts at:   1187    1187 
   Size:       4367
 
PstI C_TGCA'G

Cuts at:    149     209     227    1362    1414    2099     149 
   Size:         60      18    1135      52     685    2417

  Fragments arranged by size: 

                2417    1135     685      60      52      18
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4367
 
PvuII CAG'CTG

Cuts at:   1792    2152    1792 
   Size:        360    4007
 
RsrII CG'GwC_CG

Cuts at:   2562    2562 
   Size:       4367
 
SacI G_AGCT'C

Cuts at:   1381    1381 
   Size:       4367
 
SacII CC_GC'GG

Cuts at:    185     395     185 
   Size:        210    4157
 
SalI G'TCGA_C

Cuts at:   1404    1404 
   Size:       4367
 
SapI GCTCTTCn'nnn_

Cuts at:   2390    2600    2390 
   Size:        210    4157
 
SbfI CC_TGCA'GG

Cuts at:   1414    1414 
   Size:       4367
 
ScaI AGT'ACT

Cuts at:   1153    3884    1153 
   Size:       2731    1636
 
SfcI C'TryA_G

Cuts at:     33     145     205     223    1358    1410    2095    3063    3254 
   Size:        112      60      18    1135      52     685     968     191

Cuts at:   3254      33 
   Size:       1146

  Fragments arranged by size: 

                1146    1135     968     685     191     112      60      52
                  18
 
SfoI GGC'GCC

Cuts at:    294     389    1799    2047     294 
   Size:         95    1410     248    2614

  Fragments arranged by size: 

                2614    1410     248      95
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4367
 
SmaI CCC'GGG

Cuts at:   1389    1389 
   Size:       4367
 
SmlI C'TyrA_G

Cuts at:    599    1187    1522    2437    3151    3413     599 
   Size:        588     335     915     714     262    1553

  Fragments arranged by size: 

                1553     915     714     588     335     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4367
 
SphI G_CATG'C

Cuts at:   1420    1920    1420 
   Size:        500    3867
 
StyI C'CwwG_G

Cuts at:   1501    1855    1501 
   Size:        354    4013
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    813    1950    2083    2542    2751     813 
   Size:       1137     133     459     209    2429

  Fragments arranged by size: 

                2429    1137     459     209     133
 
TatI w'GTAC_w

Cuts at:    788    1151    3882     788 
   Size:        363    2731    1273

  Fragments arranged by size: 

                2731    1273     363
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    917     949    3884    3916     917 
   Size:         32    2935      32    1368

  Fragments arranged by size: 

                2935    1368      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2164    2164 
   Size:       4367
 
XbaI T'CTAG_A

Cuts at:   1398    1398 
   Size:       4367
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1862    1862 
   Size:       4367
 
XhoI C'TCGA_G

Cuts at:   1187    1187 
   Size:       4367
 
XmaI C'CCGG_G

Cuts at:   1387    1387 
   Size:       4367
 
XmnI GAAnn'nnTTC

Cuts at:   1256    1256 
   Size:       4367
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI
     PpiI    PpuMI     PsiI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI
    BmgBI     BmtI    BsiWI     BsmI    BsmBI   Bsu36I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI
    PshAI   PspOMI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     ZraI
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