Restriction map for pF1KB3078
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbrPI       BbsI        BbvCI       BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoRI       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MfeI        
MluI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         2086  GTCTAC               GT^[AC][GT]AC
AcsI         1279  AAATTT               [GA]^AATT[CT]
Alw44I        877  GTGCAC               G^TGCAC
ApaI         1048  GGGCCC               GGGCC^C
ApaLI         877  GTGCAC               G^TGCAC
ApoI         1279  AAATTT               [GA]^AATT[CT]
Asp700I       342  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI         2066  GCAAGTACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1829  GTATCC               GTATCC(6/5)
BclI         1478  TGATCA               T^GATCA
BfaI          487  CTAG                 C^TAG
BglI         2360  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1427  AGATCT               A^GATCT
BsaBI        2575  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         805  GAGGAG               GAGGAG(10/8)
BsrDI         423  CATTGC               GCAATG(2/0)
BsrFI        1139  GCCGGC               [GA]^CCGG[CT]
BssHII         61  GCGCGC               G^CGCGC
Cfr10I       1139  GCCGGC               [GA]^CCGG[CT]
CpoI          520  CGGACCG              CG^G[AT]CCG
DraI         2559  TTTAAA               TTT^AAA
DraIII        315  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          142  CGGCCG               C^GGCCG
EclXI         142  CGGCCG               C^GGCCG
Eco52I        142  CGGCCG               C^GGCCG
EcoRV        1912  GATATC               GAT^ATC
FbaI         1478  TGATCA               T^GATCA
KspI           10  CCGCGG               CCGC^GG
MaeI          487  CTAG                 C^TAG
MamI         2575  GATCAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1139  GCCGGC               GCC^GGC
NdeI         1954  CATATG               CA^TATG
NgoMIV       1139  GCCGGC               G^CCGGC
PpuMI        1666  AGGACCT              [GA]G^G[AT]CC[CT]
RsrII         520  CGGACCG              CG^G[AT]CCG
SacII          10  CCGCGG               CCGC^GG
SmlI          568  CTCAAG               C^T[CT][GA]AG
XcmI         2717  CCATCGGAGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          342  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII        908  TCCGGA               T^CCGGA
             1250  TCCGGA              
AflIII        812  ACATGT               A^C[GA][CT]GT
             2784  ACATGT              
BbeI          105  GGCGCC               GGCGC^C
              133  GGCGCC              
BseAI         908  TCCGGA               T^CCGGA
             1250  TCCGGA              
BsgI          454  GTGCAG               GTGCAG(16/14)
             2155  GTGCAG              
BsiEI         142  CGGCCG               CG[GA][CT]^CG
             1694  CGACCG              
BspEI         908  TCCGGA               T^CCGGA
             1250  TCCGGA              
BspLU11I      812  ACATGT               A^CATGT
             2784  ACATGT              
BspMI         299  ACCTGC               ACCTGC(4/8)
              873  GCAGGT              
BsrBI         163  CCGCTC               CCGCTC(-3/-3)
              187  CCGCTC              
BstEII       2328  GGTCACC              G^GT[ACGT]ACC
             2433  GGTCACC             
BstPI        2328  GGTCACC              G^GT[ACGT]ACC
             2433  GGTCACC             
BstXI         194  CCAAGGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1403  CCATCATCCTGG        
EarI         1498  CTCTTC               CTCTTC(1/4)
             2428  GAAGAG              
EcoO65I      2328  GGTCACC              G^GT[ACGT]ACC
             2433  GGTCACC             
HaeII         105  GGCGCC               [GA]GCGC^[CT]
              133  GGCGCC              
KasI          105  GGCGCC               G^GCGCC
              133  GGCGCC              
Ksp632I      1498  CTCTTC               CTCTTC(1/4)
             2428  GAAGAG              
MroI          908  TCCGGA               T^CCGGA
             1250  TCCGGA              
NarI          105  GGCGCC               GG^CGCC
              133  GGCGCC              
NcoI          929  CCATGG               C^CATGG
             1115  CCATGG              
SfoI          105  GGCGCC               GGC^GCC
              133  GGCGCC              
SphI         1128  GCATGC               GCATG^C
             1808  GCATGC              
Tsp509I      1280  AATT                 ^AATT
             1289  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BsaWI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI        
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