Restriction map for pF1KB3078
 (Circular) (Six-base) MAPSORT of: pF1KB3078.seq  Check: 958  from: 1  to: 5936



pF1KB3078  5936 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    972     972 
   Size:       5936
 
AccI GT'mk_AC

Cuts at:   2195    2974    2195 
   Size:        779    5157
 
Acc65I G'GTAC_C

Cuts at:   2952    2952 
   Size:       5936
 
AclI AA'CG_TT

Cuts at:   5776    5776 
   Size:       5936
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     856    3761    4193    4546    5208     688 
   Size:        168    2905     432     353     662    1416

  Fragments arranged by size: 

                2905    1416     662     432     353     168
 
AflIII A'CryG_T

Cuts at:    920    2892    3288    5088    5369     920 
   Size:       1972     396    1800     281    1487

  Fragments arranged by size: 

                1972    1800    1487     396     281
 
AleI CACnn'nnGTG

Cuts at:   2443    2443 
   Size:       5936
 
AlwNI CAG_nnn'CTG

Cuts at:   1778    1896    2858    3241    4679    1778 
   Size:        118     962     383    1438    3035

  Fragments arranged by size: 

                3035    1438     962     383     118
 
ApaI G_GGCC'C

Cuts at:   1160    1160 
   Size:       5936
 
ApaLI G'TGCA_C

Cuts at:    985    4774     985 
   Size:       3789    2147
 
ApoI r'AATT_y

Cuts at:   1387    2940    5289    5545    1387 
   Size:       1553    2349     256    1778

  Fragments arranged by size: 

                2349    1778    1553     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5936
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5936
 
AvaI C'yCGr_G

Cuts at:    136     217    1579    2246    2922    2956     136 
   Size:         81    1362     667     676      34    3116

  Fragments arranged by size: 

                3116    1362     676     667      81      34
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1920    1953    1920 
   Size:         33    5903
 
BamHI G'GATC_C

Cuts at:   1259    1486    1729    2961    1259 
   Size:        227     243    1232    4234

  Fragments arranged by size: 

                4234    1232     243     227
 
BanI G'GyrC_C

Cuts at:    213     241    1250    2952    3366    3614    3649     213 
   Size:         28    1009    1702     414     248      35    2500

  Fragments arranged by size: 

                2500    1702    1009     414     248      35      28
 
BanII G_rGCy'C

Cuts at:    127    1070    1078    1160    2609    2720    2950    3980     127 
   Size:        943       8      82    1449     111     230    1030    2083

  Fragments arranged by size: 

                2083    1449    1030     943     230     111      82       8
            
 
BbeI G_GCGC'C

Cuts at:    217     245    3370    3618     217 
   Size:         28    3125     248    2535

  Fragments arranged by size: 

                3125    2535     248      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2163    2197    2163 
   Size:         34    5902
 
BciVI GTATCCnnnnn_n'

Cuts at:   1948    3834    4890    5936    1948 
   Size:       1886    1056    1046    1948

  Fragments arranged by size: 

                1948    1886    1056    1046
 
BclI T'GATC_A

Cuts at:   1586    3456    1586 
   Size:       1870    4066
 
BglI GCCn_nnn'nGGC

Cuts at:   2474    2474 
   Size:       5936
 
BglII A'GATC_T

Cuts at:   1535    3451    5488    1535 
   Size:       1916    2037    1983

  Fragments arranged by size: 

                2037    1983    1916
 
BlpI GC'TnA_GC

Cuts at:   3048    3048 
   Size:       5936
 
Bme1580I G_kGCm'C

Cuts at:    706     989    1160    3561    3654    4778     706 
   Size:        283     171    2401      93    1124    1864

  Fragments arranged by size: 

                2401    1864    1124     283     171      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   3148    3229    3564    5708    3148 
   Size:         81     335    2144    3376

  Fragments arranged by size: 

                3376    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    819    1708    4303     819 
   Size:        889    2595    2452

  Fragments arranged by size: 

                2595    2452     889
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    776    1474    1540    1854    2062    2491    2744    2773     776 
   Size:        698      66     314     208     429     253      29    3939

  Fragments arranged by size: 

                3939     698     429     314     253     208      66      29
            
 
Bpu10I CC'TnA_GC

Cuts at:   1072    1072 
   Size:       5936
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    661    3112    3991    4705    5003     661 
   Size:       2451     879     714     298    1594

  Fragments arranged by size: 

                2451    1594     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1315    2131    2925    5640    1315 
   Size:        816     794    2715    1611

  Fragments arranged by size: 

                2715    1611     816     794
 
BsaAI yAC'GTr

Cuts at:   3289    3289 
   Size:       5936
 
BsaBI GATnn'nnATC

Cuts at:      6    2687    3450    5487       6 
   Size:       2681     763    2037     455

  Fragments arranged by size: 

                2681    2037     763     455
 
BsaHI Gr'CG_yC

Cuts at:    214     242     506    2840    3367    3615    4317    5813     214 
   Size:         28     264    2334     527     248     702    1496     337

  Fragments arranged by size: 

                2334    1496     702     527     337     264     248      28
            
 
BsaWI w'CCGG_w

Cuts at:   1016    1358    1625    3125    3646    4324    4735    4882    5156 
   Size:        342     267    1500     521     678     411     147     274

Cuts at:   5156    1016 
   Size:       1796

  Fragments arranged by size: 

                1796    1500     678     521     411     342     274     267
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    907     937     907 
   Size:         30    5906
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    928     928 
   Size:       5936
 
BseYI C'CCAG_C

Cuts at:    230     259    1159    1290    2007    2693    3361    4119    4784 
   Size:         29     900     131     717     686     668     758     665

Cuts at:   4784    5192     230 
   Size:        408     974

  Fragments arranged by size: 

                 974     900     758     717     686     668     665     408
                 131      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    583    2284     583 
   Size:       1701    4235
 
BsiEI CG_ry'CG

Cuts at:    104     253    1805    3478    3524    4754     104 
   Size:        149    1552    1673      46    1230    1286

  Fragments arranged by size: 

                1673    1552    1286    1230     149      46
 
BsiHKAI G_wGCw'C

Cuts at:    127     341     989    1078    2048    2609    2647    2950    3728 
   Size:        214     648      89     970     561      38     303     778

Cuts at:   3728    3918    4778     127 
   Size:        190     860    1285

  Fragments arranged by size: 

                1285     970     860     778     648     561     303     214
                 190      89      38
 
Bsp1286I G_dGCh'C

Cuts at:    127     341     706     989    1070    1078    1160    2048    2609 
   Size:        214     365     283      81       8      82     888     561

Cuts at:   2609    2647    2720    2950    3561    3654    3728    3918    3980 
   Size:         38      73     230     611      93      74     190      62

Cuts at:   3980    4778     127 
   Size:        798    1285

  Fragments arranged by size: 

                1285     888     798     611     561     365     283     230
                 214     190      93      82      81      74      73      62
                  38       8
 
BspEI T'CCGG_A

Cuts at:   1016    1358    1016 
   Size:        342    5594
 
BspHI T'CATG_A

Cuts at:   4368    4368 
   Size:       5936
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    416     972    2986    3200    3502    3883     416 
   Size:        556    2014     214     302     381    2469

  Fragments arranged by size: 

                2469    2014     556     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     273     297    4228    4282    5766    5931      50 
   Size:        223      24    3931      54    1484     165      55

  Fragments arranged by size: 

                3931    1484     223     165      55      54      24
 
BsrDI GCAATG_nn'

Cuts at:    530    3848     530 
   Size:       3318    2618
 
BsrFI r'CCGG_y

Cuts at:   1247    3934    4115    1247 
   Size:       2687     181    3068

  Fragments arranged by size: 

                3068    2687     181
 
BssHII G'CGCG_C

Cuts at:    169     169 
   Size:       5936
 
BssSI C'ACGA_G

Cuts at:   4207    4915    4207 
   Size:        708    5228
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3313    3313 
   Size:       5936
 
BstBI TT'CG_AA

Cuts at:   4297    4297 
   Size:       5936
 
BstEII G'GTnAC_C

Cuts at:   2436    2541    2436 
   Size:        105    5831
 
BstXI CCAn_nnnn'nTGG

Cuts at:    309    1518     309 
   Size:       1209    4727
 
BstYI r'GATC_y

Cuts at:      1     368    1259    1486    1535    1729    2736    2961    3428 
   Size:        367     891     227      49     194    1007     225     467

Cuts at:   3428    3451    3786    4032    4338    4350    4436    5488       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052    1007     891     467     449     367     335     306
                 246     227     225     194      86      49      23      12
            
 
BtgI C'CryG_G

Cuts at:    107     118     347    1037    1170    1223    1790     107 
   Size:         11     229     690     133      53     567    4253

  Fragments arranged by size: 

                4253     690     567     229     133      53      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1185    2479    3886    4067    4230    1185 
   Size:       1294    1407     181     163    2891

  Fragments arranged by size: 

                2891    1407    1294     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1210    1231    3179    1210 
   Size:         21    1948    3967

  Fragments arranged by size: 

                3967    1948      21
 
DraI TTT'AAA

Cuts at:   2669    2935    2669 
   Size:        266    5670
 
DraIII CAC_nnn'GTG

Cuts at:    428     428 
   Size:       5936
 
DrdI GACnn_nn'nnGTC

Cuts at:   3642    4986    3642 
   Size:       1344    4592
 
EaeI y'GGCC_r

Cuts at:    250     311     356     864    1442    1592    3521    3695    4086 
   Size:         61      45     508     578     150    1929     174     391

Cuts at:   4086    4113    5458     250 
   Size:         27    1345     728

  Fragments arranged by size: 

                1929    1345     728     578     508     391     174     150
                  61      45      27
 
EagI C'GGCC_G

Cuts at:    250    3521     250 
   Size:       3271    2665
 
EarI CTCTTCn'nnn_

Cuts at:   1612    2531    3959    4169    1612 
   Size:        919    1428     210    3379

  Fragments arranged by size: 

                3379    1428     919     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2020    4144    4886    5032    5746    2020 
   Size:       2124     742     146     714    2210

  Fragments arranged by size: 

                2210    2124     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    125    1076    2607    2948     125 
   Size:        951    1531     341    3113

  Fragments arranged by size: 

                3113    1531     951     341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     776     856    1474    1540    1854    2062    2491    2744 
   Size:         88      80     618      66     314     208     429     253

Cuts at:   2744    2773    3761    4193    4546    5208     688 
   Size:         29     988     432     353     662    1416

  Fragments arranged by size: 

                1416     988     662     618     432     429     353     314
                 253     208      88      80      66      29
 
EcoO109I rG'GnC_Cy

Cuts at:    221    1156    1775    2475    3075     221 
   Size:        935     619     700     600    3082

  Fragments arranged by size: 

                3082     935     700     619     600
 
EcoRI G'AATT_C

Cuts at:   2940    2940 
   Size:       5936
 
EcoRV GAT'ATC

Cuts at:   2022    2022 
   Size:       5936
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3955    3955 
   Size:       5936
 
FspI TGC'GCA

Cuts at:   3717    3717 
   Size:       5936
 
HaeII r_GCGC'y

Cuts at:    217     245    3370    3618    4848    5619     217 
   Size:         28    3125     248    1230     771     534

  Fragments arranged by size: 

                3125    1230     771     534     248      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1638    1670    1721    1753    2006    2038    2740    2772    2903 
   Size:         32      51      32     253      32     702      32     131

Cuts at:   2903    2935    3778    3808    3810    3840    1638 
   Size:         32     843      30       2      30    3734

  Fragments arranged by size: 

                3734     843     702     253     131      51      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   2975    2975 
   Size:       5936
 
KasI G'GCGC_C

Cuts at:    213     241    3366    3614     213 
   Size:         28    3125     248    2535

  Fragments arranged by size: 

                3125    2535     248      28
 
KpnI G_GTAC'C

Cuts at:   2956    2956 
   Size:       5936
 
MluI A'CGCG_T

Cuts at:   5369    5369 
   Size:       5936
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    830    4695    4879     830 
   Size:       3865     184    1887

  Fragments arranged by size: 

                3865    1887     184
 
MscI TGG'CCA

Cuts at:    313     358     866    1594    3697     313 
   Size:         45     508     728    2103    2552

  Fragments arranged by size: 

                2552    2103     728     508      45
 
MslI CAynn'nnrTG

Cuts at:   1054    1645    2062    2185    2443    2891    3925    4052    1054 
   Size:        591     417     123     258     448    1034     127    2938

  Fragments arranged by size: 

                2938    1034     591     448     417     258     127     123
            
 
MspA1I CmG'CkG

Cuts at:    120     198    1010    1257    1527    2230    2553    2846    3047 
   Size:         78     812     247     270     703     323     293     201

Cuts at:   3047    3361    3721    4505    4750     120 
   Size:        314     360     784     245    1306

  Fragments arranged by size: 

                1306     812     784     703     360     323     314     293
                 270     247     245     201      78
 
NaeI GCC'GGC

Cuts at:   1249    4117    1249 
   Size:       2868    3068
 
NarI GG'CG_CC

Cuts at:    214     242    3367    3615     214 
   Size:         28    3125     248    2535

  Fragments arranged by size: 

                3125    2535     248      28
 
NcoI C'CATG_G

Cuts at:    107    1037    1223     107 
   Size:        930     186    4820

  Fragments arranged by size: 

                4820     930     186
 
NdeI CA'TA_TG

Cuts at:   2063    2063 
   Size:       5936
 
NgoMIV G'CCGG_C

Cuts at:   1247    4115    1247 
   Size:       2868    3068
 
NspI r_CATG'y

Cuts at:    924    1240    1920    2732    2896    2989    3489    5092     924 
   Size:        316     680     812     164      93     500    1603    1768

  Fragments arranged by size: 

                1768    1603     812     680     500     316     164      93
            
 
PciI A'CATG_T

Cuts at:    920    2892    5088     920 
   Size:       1972    2196    1768

  Fragments arranged by size: 

                2196    1972    1768
 
PflMI CCAn_nnn'nTGG

Cuts at:    492     882    1290     492 
   Size:        390     408    5138

  Fragments arranged by size: 

                5138     408     390
 
PfoI T'CCnGG_A

Cuts at:   1262    1517    2039    1262 
   Size:        255     522    5159

  Fragments arranged by size: 

                5159     522     255
 
PmeI GTTT'AAAC

Cuts at:   2935    2935 
   Size:       5936
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4382    4414    4382 
   Size:         32    5904
 
PpuMI rG'GwC_Cy

Cuts at:   1775    1775 
   Size:       5936
 
PspOMI G'GGCC_C

Cuts at:   1156    1156 
   Size:       5936
 
PstI C_TGCA'G

Cuts at:   1217    2302    2558    2983    3668    1217 
   Size:       1085     256     425     685    3485

  Fragments arranged by size: 

                3485    1085     685     425     256
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5936
 
PvuII CAG'CTG

Cuts at:   1527    2553    2846    3361    3721    1527 
   Size:       1026     293     515     360    3742

  Fragments arranged by size: 

                3742    1026     515     360     293
 
RsrII CG'GwC_CG

Cuts at:    629    4131     629 
   Size:       3502    2434
 
SacI G_AGCT'C

Cuts at:    127    1078    2609    2950     127 
   Size:        951    1531     341    3113

  Fragments arranged by size: 

                3113    1531     951     341
 
SacII CC_GC'GG

Cuts at:    121     121 
   Size:       5936
 
SalI G'TCGA_C

Cuts at:   2973    2973 
   Size:       5936
 
SapI GCTCTTCn'nnn_

Cuts at:   3959    4169    3959 
   Size:        210    5726
 
SbfI CC_TGCA'GG

Cuts at:   2983    2983 
   Size:       5936
 
ScaI AGT'ACT

Cuts at:   5453    5453 
   Size:       5936
 
SfcI C'TryA_G

Cuts at:     33    1213    1551    2298    2479    2554    2698    2741    2979 
   Size:       1180     338     747     181      75     144      43     238

Cuts at:   2979    3664    4632    4823      33 
   Size:        685     968     191    1146

  Fragments arranged by size: 

                1180    1146     968     747     685     338     238     191
                 181     144      75      43
 
SfoI GGC'GCC

Cuts at:    215     243    3368    3616     215 
   Size:         28    3125     248    2535

  Fragments arranged by size: 

                3125    2535     248      28
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5936
 
SmaI CCC'GGG

Cuts at:    138     219    2248    2958     138 
   Size:         81    2029     710    3116

  Fragments arranged by size: 

                3116    2029     710      81
 
SmlI C'TyrA_G

Cuts at:    676    3091    4006    4720    4982     676 
   Size:       2415     915     714     262    1630

  Fragments arranged by size: 

                2415    1630     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5936
 
SphI G_CATG'C

Cuts at:   1240    1920    2989    3489    1240 
   Size:        680    1069     500    3687

  Fragments arranged by size: 

                3687    1069     680     500
 
SrfI GCCC'GGGC

Cuts at:    138     138 
   Size:       5936
 
StyI C'CwwG_G

Cuts at:    107     302     314     546     861    1037    1223    1482    1680 
   Size:        195      12     232     315     176     186     259     198

Cuts at:   1680    3070    3424     107 
   Size:       1390     354    2619

  Fragments arranged by size: 

                2619    1390     354     315     259     232     198     195
                 186     176      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2074    2424    3519    3652    4111    4320    2074 
   Size:        350    1095     133     459     209    3690

  Fragments arranged by size: 

                3690    1095     459     350     209     133
 
TatI w'GTAC_w

Cuts at:   1130    1430    1988    2177    5451    1130 
   Size:        300     558     189    3274    1615

  Fragments arranged by size: 

                3274    1615     558     300     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2891    2923    5453    5485    2891 
   Size:         32    2530      32    3342

  Fragments arranged by size: 

                3342    2530      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3733    3733 
   Size:       5936
 
XbaI T'CTAG_A

Cuts at:   2967    2967 
   Size:       5936
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2832    3431    2832 
   Size:        599    5337
 
XmaI C'CCGG_G

Cuts at:    136     217    2246    2956     136 
   Size:         81    2029     710    3116

  Fragments arranged by size: 

                3116    2029     710      81
 
XmnI GAAnn'nnTTC

Cuts at:    454     454 
   Size:       5936
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI     AscI
    AvrII     BbsI    BbvCI    BfrBI    BmgBI     BmtI    BsiWI     BsmI
    BsmBI    BsrGI  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI     FseI
    FspAI  HindIII     HpaI     MfeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmlI    PshAI     PsiI    PspXI     PsrI    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SspI     StuI     SwaI     XhoI
     ZraI
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