Restriction map for pF1KB3078
 (Linear) (Six-base) MAPSORT of: KIBB3078.seq  Check: 6092  from: 1  to: 2823



KIBB3078.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     864    2823 
   Size:        864    1959
 
AccI GT'mk_AC

Cuts at:      0    2087    2823 
   Size:       2087     736
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     748    2823 
   Size:        580     168    2075

  Fragments arranged by size: 

                2075     580     168
 
AflIII A'CryG_T

Cuts at:      0     812    2784    2823 
   Size:        812    1972      39

  Fragments arranged by size: 

                1972     812      39
 
AleI CACnn'nnGTG

Cuts at:      0    2335    2823 
   Size:       2335     488
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1670    1788    2750    2823 
   Size:       1670     118     962      73

  Fragments arranged by size: 

                1670     962     118      73
 
ApaI G_GGCC'C

Cuts at:      0    1052    2823 
   Size:       1052    1771
 
ApaLI G'TGCA_C

Cuts at:      0     877    2823 
   Size:        877    1946
 
ApoI r'AATT_y

Cuts at:      0    1279    2823 
   Size:       1279    1544
 
AvaI C'yCGr_G

Cuts at:      0      28     109    1471    2138    2814    2823 
   Size:         28      81    1362     667     676       9

  Fragments arranged by size: 

                1362     676     667      81      28       9
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1812    1845    2823 
   Size:       1812      33     978

  Fragments arranged by size: 

                1812     978      33
 
BamHI G'GATC_C

Cuts at:      0    1151    1378    1621    2823 
   Size:       1151     227     243    1202

  Fragments arranged by size: 

                1202    1151     243     227
 
BanI G'GyrC_C

Cuts at:      0     105     133    1142    2823 
   Size:        105      28    1009    1681

  Fragments arranged by size: 

                1681    1009     105      28
 
BanII G_rGCy'C

Cuts at:      0      19     962     970    1052    2501    2612    2823 
   Size:         19     943       8      82    1449     111     211

  Fragments arranged by size: 

                1449     943     211     111      82      19       8
 
BbeI G_GCGC'C

Cuts at:      0     109     137    2823 
   Size:        109      28    2686

  Fragments arranged by size: 

                2686     109      28
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2055    2089    2823 
   Size:       2055      34     734

  Fragments arranged by size: 

                2055     734      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1840    2823 
   Size:       1840     983
 
BclI T'GATC_A

Cuts at:      0    1478    2823 
   Size:       1478    1345
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2366    2823 
   Size:       2366     457
 
BglII A'GATC_T

Cuts at:      0    1427    2823 
   Size:       1427    1396
 
Bme1580I G_kGCm'C

Cuts at:      0     598     881    1052    2823 
   Size:        598     283     171    1771

  Fragments arranged by size: 

                1771     598     283     171
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     711    1600    2823 
   Size:        711     889    1223

  Fragments arranged by size: 

                1223     889     711
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     668    1366    1432    1746    1954    2383    2636    2665 
   Size:        668     698      66     314     208     429     253      29

Cuts at:   2665    2823 
   Size:        158

  Fragments arranged by size: 

                 698     668     429     314     253     208     158      66
                  29
 
Bpu10I CC'TnA_GC

Cuts at:      0     964    2823 
   Size:        964    1859
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553    2823 
   Size:        553    2270
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1207    2023    2817    2823 
   Size:       1207     816     794       6

  Fragments arranged by size: 

                1207     816     794       6
 
BsaBI GATnn'nnATC

Cuts at:      0    2579    2823 
   Size:       2579     244
 
BsaHI Gr'CG_yC

Cuts at:      0     106     134     398    2732    2823 
   Size:        106      28     264    2334      91

  Fragments arranged by size: 

                2334     264     106      91      28
 
BsaWI w'CCGG_w

Cuts at:      0     908    1250    1517    2823 
   Size:        908     342     267    1306

  Fragments arranged by size: 

                1306     908     342     267
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     799     829    2823 
   Size:        799      30    1994

  Fragments arranged by size: 

                1994     799      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     820    2823 
   Size:        820    2003
 
BseYI C'CCAG_C

Cuts at:      0     122     151    1051    1182    1899    2585    2823 
   Size:        122      29     900     131     717     686     238

  Fragments arranged by size: 

                 900     717     686     238     131     122      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    2176    2823 
   Size:        475    1701     647

  Fragments arranged by size: 

                1701     647     475
 
BsiEI CG_ry'CG

Cuts at:      0     145    1697    2823 
   Size:        145    1552    1126

  Fragments arranged by size: 

                1552    1126     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     233     881     970    1940    2501    2539    2823 
   Size:         19     214     648      89     970     561      38     284

  Fragments arranged by size: 

                 970     648     561     284     214      89      38      19
            
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     233     598     881     962     970    1052    1940 
   Size:         19     214     365     283      81       8      82     888

Cuts at:   1940    2501    2539    2612    2823 
   Size:        561      38      73     211

  Fragments arranged by size: 

                 888     561     365     283     214     211      82      81
                  73      38      19       8
 
BspEI T'CCGG_A

Cuts at:      0     908    1250    2823 
   Size:        908     342    1573

  Fragments arranged by size: 

                1573     908     342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     308     864    2823 
   Size:        308     556    1959

  Fragments arranged by size: 

                1959     556     308
 
BsrBI CCG'CTC

Cuts at:      0     165     189    2823 
   Size:        165      24    2634

  Fragments arranged by size: 

                2634     165      24
 
BsrDI GCAATG_nn'

Cuts at:      0     422    2823 
   Size:        422    2401
 
BsrFI r'CCGG_y

Cuts at:      0    1139    2823 
   Size:       1139    1684
 
BssHII G'CGCG_C

Cuts at:      0      61    2823 
   Size:         61    2762
 
BstEII G'GTnAC_C

Cuts at:      0    2328    2433    2823 
   Size:       2328     105     390

  Fragments arranged by size: 

                2328     390     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201    1410    2823 
   Size:        201    1209    1413

  Fragments arranged by size: 

                1413    1209     201
 
BstYI r'GATC_y

Cuts at:      0     260    1151    1378    1427    1621    2628    2823 
   Size:        260     891     227      49     194    1007     195

  Fragments arranged by size: 

                1007     891     260     227     195     194      49
 
BtgI C'CryG_G

Cuts at:      0      10     239     929    1062    1115    1682    2823 
   Size:         10     229     690     133      53     567    1141

  Fragments arranged by size: 

                1141     690     567     229     133      53      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1077    2371    2823 
   Size:       1077    1294     452

  Fragments arranged by size: 

                1294    1077     452
 
BtsI GCAGTG_nn'

Cuts at:      0    1102    1123    2823 
   Size:       1102      21    1700

  Fragments arranged by size: 

                1700    1102      21
 
DraI TTT'AAA

Cuts at:      0    2561    2823 
   Size:       2561     262
 
DraIII CAC_nnn'GTG

Cuts at:      0     320    2823 
   Size:        320    2503
 
EaeI y'GGCC_r

Cuts at:      0     142     203     248     756    1334    1484    2823 
   Size:        142      61      45     508     578     150    1339

  Fragments arranged by size: 

                1339     578     508     150     142      61      45
 
EagI C'GGCC_G

Cuts at:      0     142    2823 
   Size:        142    2681
 
EarI CTCTTCn'nnn_

Cuts at:      0    1504    2423    2823 
   Size:       1504     919     400

  Fragments arranged by size: 

                1504     919     400
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1912    2823 
   Size:       1912     911
 
EcoICRI GAG'CTC

Cuts at:      0      17     968    2499    2823 
   Size:         17     951    1531     324

  Fragments arranged by size: 

                1531     951     324      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     668     748    1366    1432    1746    1954    2383 
   Size:        580      88      80     618      66     314     208     429

Cuts at:   2383    2636    2665    2823 
   Size:        253      29     158

  Fragments arranged by size: 

                 618     580     429     314     253     208     158      88
                  80      66      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     113    1048    1667    2367    2823 
   Size:        113     935     619     700     456

  Fragments arranged by size: 

                 935     700     619     456     113
 
EcoRV GAT'ATC

Cuts at:      0    1914    2823 
   Size:       1914     909
 
HaeII r_GCGC'y

Cuts at:      0     109     137    2823 
   Size:        109      28    2686

  Fragments arranged by size: 

                2686     109      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1530    1562    1613    1645    1898    1930    2632    2664 
   Size:       1530      32      51      32     253      32     702      32

Cuts at:   2664    2795    2823 
   Size:        131      28

  Fragments arranged by size: 

                1530     702     253     131      51      32      32      32
                  32      28
 
KasI G'GCGC_C

Cuts at:      0     105     133    2823 
   Size:        105      28    2690

  Fragments arranged by size: 

                2690     105      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    2823 
   Size:        722    2101
 
MscI TGG'CCA

Cuts at:      0     205     250     758    1486    2823 
   Size:        205      45     508     728    1337

  Fragments arranged by size: 

                1337     728     508     205      45
 
MslI CAynn'nnrTG

Cuts at:      0     946    1537    1954    2077    2335    2783    2823 
   Size:        946     591     417     123     258     448      40

  Fragments arranged by size: 

                 946     591     448     417     258     123      40
 
MspA1I CmG'CkG

Cuts at:      0      12      90     902    1149    1419    2122    2445    2738 
   Size:         12      78     812     247     270     703     323     293

Cuts at:   2738    2823 
   Size:         85

  Fragments arranged by size: 

                 812     703     323     293     270     247      85      78
                  12
 
NaeI GCC'GGC

Cuts at:      0    1141    2823 
   Size:       1141    1682
 
NarI GG'CG_CC

Cuts at:      0     106     134    2823 
   Size:        106      28    2689

  Fragments arranged by size: 

                2689     106      28
 
NcoI C'CATG_G

Cuts at:      0     929    1115    2823 
   Size:        929     186    1708

  Fragments arranged by size: 

                1708     929     186
 
NdeI CA'TA_TG

Cuts at:      0    1955    2823 
   Size:       1955     868
 
NgoMIV G'CCGG_C

Cuts at:      0    1139    2823 
   Size:       1139    1684
 
NspI r_CATG'y

Cuts at:      0     816    1132    1812    2624    2788    2823 
   Size:        816     316     680     812     164      35

  Fragments arranged by size: 

                 816     812     680     316     164      35
 
PciI A'CATG_T

Cuts at:      0     812    2784    2823 
   Size:        812    1972      39

  Fragments arranged by size: 

                1972     812      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     384     774    1182    2823 
   Size:        384     390     408    1641

  Fragments arranged by size: 

                1641     408     390     384
 
PfoI T'CCnGG_A

Cuts at:      0    1154    1409    1931    2823 
   Size:       1154     255     522     892

  Fragments arranged by size: 

                1154     892     522     255
 
PpuMI rG'GwC_Cy

Cuts at:      0    1667    2823 
   Size:       1667    1156
 
PspOMI G'GGCC_C

Cuts at:      0    1048    2823 
   Size:       1048    1775
 
PstI C_TGCA'G

Cuts at:      0    1109    2194    2450    2823 
   Size:       1109    1085     256     373

  Fragments arranged by size: 

                1109    1085     373     256
 
PvuII CAG'CTG

Cuts at:      0    1419    2445    2738    2823 
   Size:       1419    1026     293      85

  Fragments arranged by size: 

                1419    1026     293      85
 
RsrII CG'GwC_CG

Cuts at:      0     521    2823 
   Size:        521    2302
 
SacI G_AGCT'C

Cuts at:      0      19     970    2501    2823 
   Size:         19     951    1531     322

  Fragments arranged by size: 

                1531     951     322      19
 
SacII CC_GC'GG

Cuts at:      0      13    2823 
   Size:         13    2810
 
SfcI C'TryA_G

Cuts at:      0    1105    1443    2190    2371    2446    2590    2633    2823 
   Size:       1105     338     747     181      75     144      43     190

  Fragments arranged by size: 

                1105     747     338     190     181     144      75      43
            
 
SfoI GGC'GCC

Cuts at:      0     107     135    2823 
   Size:        107      28    2688

  Fragments arranged by size: 

                2688     107      28
 
SmaI CCC'GGG

Cuts at:      0      30     111    2140    2823 
   Size:         30      81    2029     683

  Fragments arranged by size: 

                2029     683      81      30
 
SmlI C'TyrA_G

Cuts at:      0     568    2823 
   Size:        568    2255
 
SphI G_CATG'C

Cuts at:      0    1132    1812    2823 
   Size:       1132     680    1011

  Fragments arranged by size: 

                1132    1011     680
 
SrfI GCCC'GGGC

Cuts at:      0      30    2823 
   Size:         30    2793
 
StyI C'CwwG_G

Cuts at:      0     194     206     438     753     929    1115    1374    1572 
   Size:        194      12     232     315     176     186     259     198

Cuts at:   1572    2823 
   Size:       1251

  Fragments arranged by size: 

                1251     315     259     232     198     194     186     176
                  12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1966    2316    2823 
   Size:       1966     350     507

  Fragments arranged by size: 

                1966     507     350
 
TatI w'GTAC_w

Cuts at:      0    1022    1322    1880    2069    2823 
   Size:       1022     300     558     189     754

  Fragments arranged by size: 

                1022     754     558     300     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2783    2815    2823 
   Size:       2783      32       8

  Fragments arranged by size: 

                2783      32       8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2724    2823 
   Size:       2724      99
 
XmaI C'CCGG_G

Cuts at:      0      28     109    2138    2823 
   Size:         28      81    2029     685

  Fragments arranged by size: 

                2029     685      81      28
 
XmnI GAAnn'nnTTC

Cuts at:      0     346    2823 
   Size:        346    2477
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BcgI
    BciVI     BclI     BglI    BglII Bme1580I     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRV    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    RsrII     SacI
    SacII     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BbsI    BbvCI    BfrBI
     BlpI    BmgBI     BmrI     BmtI    BsaAI    BsiWI     BsmI    BsmBI
    BspHI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DrdI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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