Restriction map for pF1KB3048
 (Linear) (Six-base) MAPSORT of: KIBB3048.seq  Check: 5318  from: 1  to: 4026



KIBB3048.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3802    4026 
   Size:       3802     224
 
AatII G_ACGT'C

Cuts at:      0    1760    4026 
   Size:       1760    2266
 
AccI GT'mk_AC

Cuts at:      0     662    3381    3425    3661    4026 
   Size:        662    2719      44     236     365

  Fragments arranged by size: 

                2719     662     365     236      44
 
Acc65I G'GTAC_C

Cuts at:      0     778    1823    2186    4026 
   Size:        778    1045     363    1840

  Fragments arranged by size: 

                1840    1045     778     363
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1448    2148    3128    3583    3633    4026 
   Size:        515     933     700     980     455      50     393

  Fragments arranged by size: 

                 980     933     700     515     455     393      50
 
AfeI AGC'GCT

Cuts at:      0     734    4026 
   Size:        734    3292
 
AflIII A'CryG_T

Cuts at:      0    2042    2458    2584    3464    4026 
   Size:       2042     416     126     880     562

  Fragments arranged by size: 

                2042     880     562     416     126
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1542    3159    4026 
   Size:       1542    1617     867

  Fragments arranged by size: 

                1617    1542     867
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2936    4026 
   Size:       2936    1090
 
ApaI G_GGCC'C

Cuts at:      0    1055    1156    4026 
   Size:       1055     101    2870

  Fragments arranged by size: 

                2870    1055     101
 
ApaLI G'TGCA_C

Cuts at:      0    2169    4026 
   Size:       2169    1857
 
ApoI r'AATT_y

Cuts at:      0     291     451    1905    3999    4026 
   Size:        291     160    1454    2094      27

  Fragments arranged by size: 

                2094    1454     291     160      27
 
AseI AT'TA_AT

Cuts at:      0     902    2396    2471    4026 
   Size:        902    1494      75    1555

  Fragments arranged by size: 

                1555    1494     902      75
 
AvaI C'yCGr_G

Cuts at:      0    1223    4026 
   Size:       1223    2803
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2170    2203    4026 
   Size:       2170      33    1823

  Fragments arranged by size: 

                2170    1823      33
 
BamHI G'GATC_C

Cuts at:      0     996    4026 
   Size:        996    3030
 
BanI G'GyrC_C

Cuts at:      0     328     778    1823    2186    4026 
   Size:        328     450    1045     363    1840

  Fragments arranged by size: 

                1840    1045     450     363     328
 
BanII G_rGCy'C

Cuts at:      0    1055    1156    2161    2284    2495    3325    4026 
   Size:       1055     101    1005     123     211     830     701

  Fragments arranged by size: 

                1055    1005     830     701     211     123     101
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     556     616    1086    2225    4026 
   Size:        556      60     470    1139    1801

  Fragments arranged by size: 

                1801    1139     556     470      60
 
BbvCI CC'TCA_GC

Cuts at:      0    3728    4026 
   Size:       3728     298
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     956     990    4026 
   Size:        956      34    3036

  Fragments arranged by size: 

                3036     956      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     829    3816    4026 
   Size:        829    2987     210

  Fragments arranged by size: 

                2987     829     210
 
BglI GCCn_nnn'nGGC

Cuts at:      0     542    2060    4026 
   Size:        542    1518    1966

  Fragments arranged by size: 

                1966    1518     542
 
BlpI GC'TnA_GC

Cuts at:      0    3584    4026 
   Size:       3584     442
 
Bme1580I G_kGCm'C

Cuts at:      0     286    1055    1156    1775    2173    3552    4026 
   Size:        286     769     101     619     398    1379     474

  Fragments arranged by size: 

                1379     769     619     474     398     286     101
 
BmgBI CAC'GTC

Cuts at:      0     660    4026 
   Size:        660    3366
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     313    2843    3278    4026 
   Size:        313    2530     435     748

  Fragments arranged by size: 

                2530     748     435     313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1325    3113    3650    4026 
   Size:       1325    1788     537     376

  Fragments arranged by size: 

                1788    1325     537     376
 
Bpu10I CC'TnA_GC

Cuts at:      0    3728    4026 
   Size:       3728     298
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1336    1454    4026 
   Size:       1336     118    2572

  Fragments arranged by size: 

                2572    1336     118
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2324    4026 
   Size:       2324    1702
 
BsaAI yAC'GTr

Cuts at:      0    2585    3465    3951    4026 
   Size:       2585     880     486      75

  Fragments arranged by size: 

                2585     880     486      75
 
BsaBI GATnn'nnATC

Cuts at:      0    2879    3812    4026 
   Size:       2879     933     214

  Fragments arranged by size: 

                2879     933     214
 
BsaHI Gr'CG_yC

Cuts at:      0    1757    2927    3612    3635    4026 
   Size:       1757    1170     685      23     391

  Fragments arranged by size: 

                1757    1170     685     391      23
 
BsaWI w'CCGG_w

Cuts at:      0    1799    2076    2427    3875    4026 
   Size:       1799     277     351    1448     151

  Fragments arranged by size: 

                1799    1448     351     277     151
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     431     461    1386    1416    1624    1654    2058    2088 
   Size:        431      30     925      30     208      30     404      30

Cuts at:   2088    4026 
   Size:       1938

  Fragments arranged by size: 

                1938     925     431     404     208      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1726    2399    3730    4026 
   Size:       1726     673    1331     296

  Fragments arranged by size: 

                1726    1331     673     296
 
BseYI C'CCAG_C

Cuts at:      0     359    1331    1689    3440    4026 
   Size:        359     972     358    1751     586

  Fragments arranged by size: 

                1751     972     586     359     358
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     279    1250    1920    2286    3960    4026 
   Size:         99     180     971     670     366    1674      66

  Fragments arranged by size: 

                1674     971     670     366     180      99      66
 
BsiEI CG_ry'CG

Cuts at:      0    3388    4026 
   Size:       3388     638
 
BsiHKAI G_wGCw'C

Cuts at:      0    2173    2256    4026 
   Size:       2173      83    1770

  Fragments arranged by size: 

                2173    1770      83
 
BsiWI C'GTAC_G

Cuts at:      0    2581    4026 
   Size:       2581    1445
 
BsmI GAATG_Cn'

Cuts at:      0     354     859    1747    2236    2359    2911    2980    3148 
   Size:        354     505     888     489     123     552      69     168

Cuts at:   3148    3214    3739    3862    3900    4026 
   Size:         66     525     123      38     126

  Fragments arranged by size: 

                 888     552     525     505     489     354     168     126
                 123     123      69      66      38
 
Bsp1286I G_dGCh'C

Cuts at:      0     286    1055    1156    1775    2161    2173    2256    2284 
   Size:        286     769     101     619     386      12      83      28

Cuts at:   2284    2495    3325    3552    4026 
   Size:        211     830     227     474

  Fragments arranged by size: 

                 830     769     619     474     386     286     227     211
                 101      83      28      12
 
BspEI T'CCGG_A

Cuts at:      0    2076    2427    4026 
   Size:       2076     351    1599

  Fragments arranged by size: 

                2076    1599     351
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121     636     982    3802    4026 
   Size:        121     515     346    2820     224

  Fragments arranged by size: 

                2820     515     346     224     121
 
BsrBI CCG'CTC

Cuts at:      0      48    2846    4026 
   Size:         48    2798    1180

  Fragments arranged by size: 

                2798    1180      48
 
BsrFI r'CCGG_y

Cuts at:      0     692    3387    3473    3614    4026 
   Size:        692    2695      86     141     412

  Fragments arranged by size: 

                2695     692     412     141      86
 
BsrGI T'GTAC_A

Cuts at:      0    2983    4026 
   Size:       2983    1043
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3357    4026 
   Size:       3357     669
 
BstYI r'GATC_y

Cuts at:      0     314     996    1915    4026 
   Size:        314     682     919    2111

  Fragments arranged by size: 

                2111     919     682     314
 
BstZ17I GTA'TAC

Cuts at:      0    3382    3662    4026 
   Size:       3382     280     364

  Fragments arranged by size: 

                3382     364     280
 
Bsu36I CC'TnA_GG

Cuts at:      0    1747    4026 
   Size:       1747    2279
 
BtgI C'CryG_G

Cuts at:      0     324     668     953    3350    4026 
   Size:        324     344     285    2397     676

  Fragments arranged by size: 

                2397     676     344     324     285
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3875    4026 
   Size:       3875     151
 
BtsI GCAGTG_nn'

Cuts at:      0    1524    1903    2146    2269    2845    3259    3550    4026 
   Size:       1524     379     243     123     576     414     291     476

  Fragments arranged by size: 

                1524     576     476     414     379     291     243     123
            
 
ClaI AT'CG_AT

Cuts at:      0     966    4026 
   Size:        966    3060
 
DraIII CAC_nnn'GTG

Cuts at:      0     724    1162    3509    4026 
   Size:        724     438    2347     517

  Fragments arranged by size: 

                2347     724     517     438
 
EaeI y'GGCC_r

Cuts at:      0      69     203     252     690    1703    2577    3300    3827 
   Size:         69     134      49     438    1013     874     723     527

Cuts at:   3827    4026 
   Size:        199

  Fragments arranged by size: 

                1013     874     723     527     438     199     134      69
                  49
 
EarI CTCTTCn'nnn_

Cuts at:      0    1037    1190    4026 
   Size:       1037     153    2836

  Fragments arranged by size: 

                2836    1037     153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1261    4026 
   Size:       1261    2765
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1325    1448    2148    3113    3128    3583    3633 
   Size:        515     810     123     700     965      15     455      50

Cuts at:   3633    3650    4026 
   Size:         17     376

  Fragments arranged by size: 

                 965     810     700     515     455     376     123      50
                  17      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1051    1052    1152    1209    1793    2408    2783    4026 
   Size:       1051       1     100      57     584     615     375    1243

  Fragments arranged by size: 

                1243    1051     615     584     375     100      57       1
            
 
EcoRI G'AATT_C

Cuts at:      0    1905    4026 
   Size:       1905    2121
 
EcoRV GAT'ATC

Cuts at:      0    1509    2346    4026 
   Size:       1509     837    1680

  Fragments arranged by size: 

                1680    1509     837
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102    4026 
   Size:        102    3924
 
FspI TGC'GCA

Cuts at:      0    2007    4026 
   Size:       2007    2019
 
HaeII r_GCGC'y

Cuts at:      0     736    4026 
   Size:        736    3290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3149    3181    4026 
   Size:       3149      32     845

  Fragments arranged by size: 

                3149     845      32
 
HincII GTy'rAC

Cuts at:      0    1327    1737    3645    4026 
   Size:       1327     410    1908     381

  Fragments arranged by size: 

                1908    1327     410     381
 
HindIII A'AGCT_T

Cuts at:      0     497    1430    4026 
   Size:        497     933    2596

  Fragments arranged by size: 

                2596     933     497
 
KpnI G_GTAC'C

Cuts at:      0     782    1827    2190    4026 
   Size:        782    1045     363    1836

  Fragments arranged by size: 

                1836    1045     782     363
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1653    2048    4026 
   Size:       1653     395    1978

  Fragments arranged by size: 

                1978    1653     395
 
MscI TGG'CCA

Cuts at:      0     254    1705    3302    4026 
   Size:        254    1451    1597     724

  Fragments arranged by size: 

                1597    1451     724     254
 
MslI CAynn'nnrTG

Cuts at:      0      79    2249    3058    3452    3601    4026 
   Size:         79    2170     809     394     149     425

  Fragments arranged by size: 

                2170     809     425     394     149      79
 
MspA1I CmG'CkG

Cuts at:      0     128     140    1335    1652    1820    2193    2653    2666 
   Size:        128      12    1195     317     168     373     460      13

Cuts at:   2666    3588    4026 
   Size:        922     438

  Fragments arranged by size: 

                1195     922     460     438     373     317     168     128
                  13      12
 
NaeI GCC'GGC

Cuts at:      0    3475    3616    4026 
   Size:       3475     141     410

  Fragments arranged by size: 

                3475     410     141
 
NcoI C'CATG_G

Cuts at:      0     324     953    3350    4026 
   Size:        324     629    2397     676

  Fragments arranged by size: 

                2397     676     629     324
 
NdeI CA'TA_TG

Cuts at:      0    3624    4026 
   Size:       3624     402
 
NgoMIV G'CCGG_C

Cuts at:      0    3473    3614    4026 
   Size:       3473     141     412

  Fragments arranged by size: 

                3473     412     141
 
NspI r_CATG'y

Cuts at:      0     391    2046    2462    3543    4026 
   Size:        391    1655     416    1081     483

  Fragments arranged by size: 

                1655    1081     483     416     391
 
PasI CC'CwG_GG

Cuts at:      0    1644    4026 
   Size:       1644    2382
 
PciI A'CATG_T

Cuts at:      0    2042    2458    4026 
   Size:       2042     416    1568

  Fragments arranged by size: 

                2042    1568     416
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     325     849    2701    4026 
   Size:        325     524    1852    1325

  Fragments arranged by size: 

                1852    1325     524     325
 
PfoI T'CCnGG_A

Cuts at:      0     701    1911    2848    3356    3779    4026 
   Size:        701    1210     937     508     423     247

  Fragments arranged by size: 

                1210     937     701     508     423     247
 
PpuMI rG'GwC_Cy

Cuts at:      0    2408    2783    4026 
   Size:       2408     375    1243

  Fragments arranged by size: 

                2408    1243     375
 
PshAI GACnn'nnGTC

Cuts at:      0     275     388    2930    3841    4026 
   Size:        275     113    2542     911     185

  Fragments arranged by size: 

                2542     911     275     185     113
 
PsiI TTA'TAA

Cuts at:      0     869    4026 
   Size:        869    3157
 
PspOMI G'GGCC_C

Cuts at:      0    1051    1152    4026 
   Size:       1051     101    2874

  Fragments arranged by size: 

                2874    1051     101
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     762     794    4026 
   Size:        762      32    3232

  Fragments arranged by size: 

                3232     762      32
 
PstI C_TGCA'G

Cuts at:      0    2406    2574    3254    4026 
   Size:       2406     168     680     772

  Fragments arranged by size: 

                2406     772     680     168
 
PvuII CAG'CTG

Cuts at:      0     128     140    1335    2193    2653    3588    4026 
   Size:        128      12    1195     858     460     935     438

  Fragments arranged by size: 

                1195     935     858     460     438     128      12
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1190    4026 
   Size:       1190    2836
 
SfcI C'TryA_G

Cuts at:      0    1572    2091    2402    2570    3250    4026 
   Size:       1572     519     311     168     680     776

  Fragments arranged by size: 

                1572     776     680     519     311     168
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     542    4026 
   Size:        542    3484
 
SmaI CCC'GGG

Cuts at:      0    1225    4026 
   Size:       1225    2801
 
SmlI C'TyrA_G

Cuts at:      0    1315    1469    4026 
   Size:       1315     154    2557

  Fragments arranged by size: 

                2557    1315     154
 
SphI G_CATG'C

Cuts at:      0    3543    4026 
   Size:       3543     483
 
StuI AGG'CCT

Cuts at:      0    1147    3532    4026 
   Size:       1147    2385     494

  Fragments arranged by size: 

                2385    1147     494
 
StyI C'CwwG_G

Cuts at:      0     119     324     591     953    1190    3350    3638    4026 
   Size:        119     205     267     362     237    2160     288     388

  Fragments arranged by size: 

                2160     388     362     288     267     237     205     119
            
 
TatI w'GTAC_w

Cuts at:      0    1492    1618    2419    2819    2983    3340    4026 
   Size:       1492     126     801     400     164     357     686

  Fragments arranged by size: 

                1492     801     686     400     357     164     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     431     463    1624    1656    2112    2144    4026 
   Size:        431      32    1161      32     456      32    1882

  Fragments arranged by size: 

                1882    1161     456     431      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     709    4026 
   Size:        709    3317
 
XmaI C'CCGG_G

Cuts at:      0    1223    4026 
   Size:       1223    2803
 
ZraI GAC'GTC

Cuts at:      0    1758    4026 
   Size:       1758    2268
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI    PvuII     SapI     SfcI     SfiI     SmaI
     SmlI     SphI     StuI     StyI     TatI     TstI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI     AleI     AloI     AscI    AsiSI
    AvrII     BbeI     BclI    BfrBI    BglII     BmtI     BplI    BsmBI
    BspHI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    CspCI
     DraI     DrdI     EagI  EcoICRI    EcoNI     FseI    FspAI     HpaI
     KasI     MfeI     MluI     NarI     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmnI
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