Restriction map for pF1KB3037
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsoBI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraII       DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRV       FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SwaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwNI        1022  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1441  CCTAAGG              CC^T[ACGT]AGG
AspI         1307  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          486  TGGCCA               TGG^CCA
BbvCI        3271  GCTGAGG              CCTCAGC(-5/-2)
BcnI         2076  CCCGG                CC^[GC]GG
Bsp1407I     1095  TGTACA               T^GTACA
BsrDI        2349  CATTGC               GCAATG(2/0)
BsrGI        1095  TGTACA               T^GTACA
BstXI         222  CCATGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1441  CCTAAGG              CC^T[ACGT]AGG
CfoI         2107  GCGC                 GCG^C
DrdI         2334  GACAGCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          486  TGGCCA               [CT]^GGCC[GA]
Eco81I       1441  CCTAAGG              CC^T[ACGT]AGG
EcoT14I      3029  CCATGG               C^C[AT][AT]GG
EcoT22I       756  ATGCAT               ATGCA^T
FauI         2528  CCCGC                CCCGC(4/6)
HgaI         3285  GACGC                GACGC(5/10)
HhaI         2107  GCGC                 GCG^C
HinP1I       2107  GCGC                 G^CGC
HindIII      3427  AAGCTT               A^AGCTT
MaeII        2497  ACGT                 A^CGT
MluNI         486  TGGCCA               TGG^CCA
MscI          486  TGGCCA               TGG^CCA
NciI         2076  CCCGG                CC^[GC]GG
NcoI         3029  CCATGG               C^CATGG
NsiI          756  ATGCAT               ATGCA^T
NspI         1359  ACATGC               [GA]CATG^[CT]
PflFI        1307  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        3092  CAGCTG               CAG^CTG
SexAI         847  ACCAGGT              A^CC[AT]GGT
SpeI         1812  ACTAGT               A^CTAGT
SspBI        1095  TGTACA               T^GTACA
StuI          266  AGGCCT               AGG^CCT
StyI         3029  CCATGG               C^C[AT][AT]GG
Tth111I      1307  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2978  CCATGTATGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AgeI         1639  ACCGGT               A^CCGGT
             3260  ACCGGT              
BciVI         579  GGATAC               GTATCC(6/5)
             2478  GGATAC              
BsaBI         193  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2731  GATACTGATC          
BsaWI        1639  ACCGGT               [AT]^CCGG[AT]
             3260  ACCGGT              
BseRI        3004  GAGGAG               GAGGAG(10/8)
             3274  GAGGAG              
BsmBI        2963  GAGACG               CGTCTC(1/5)
             3283  GAGACG              
BsmI         1819  GAATGC               GAATGC(1/-1)
             2103  GAATGC              
BspMI         100  ACCTGC               ACCTGC(4/8)
             3354  ACCTGC              
Cac8I        3090  GCCAGC               GC[ACGT]^[ACGT]GC
             3403  GCAAGC              
DraI          726  TTTAAA               TTT^AAA
             1617  TTTAAA              
EcoRI         883  GAATTC               G^AATTC
             2518  GAATTC              
HincII       1635  GTTGAC               GT[CT]^[GA]AC
             2569  GTCAAC              
HindII       1635  GTTGAC               GT[CT]^[GA]AC
             2569  GTCAAC              
MamI          193  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2731  GATACTGATC          
PflMI         514  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              970  CCAGTGTTTGG         
PinAI        1639  ACCGGT               A^CCGGT
             3260  ACCGGT              
PstI         2306  CTGCAG               CTGCA^G
             2427  CTGCAG              
ScaI           28  AGTACT               AGT^ACT
             2993  AGTACT              
SmlI         1660  CTTGAG               C^T[CT][GA]AG
             1805  CTCAAG              
SspI         2556  AATATT               AAT^ATT
             3393  AATATT              
Tsp45I       1625  GTCAC                ^GT[GC]AC
             2332  GTGAC               
Van91I        514  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              970  CCAGTGTTTGG         
XbaI          912  TCTAGA               T^CTAGA
             2436  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanII       BbsI        BbvI        
BclI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      FbaI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com