Restriction map for pF1KB3037
 (Linear) (Six-base) MAPSORT of: KIBB3037.seq  Check: 3836  from: 1  to: 3450



KIBB3037.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     638    1940    2165    2449    2921    3145    3450 
   Size:        266     372    1302     225     284     472     224     305

  Fragments arranged by size: 

                1302     472     372     305     284     266     225     224
            
 
AgeI A'CCGG_T

Cuts at:      0    1639    3260    3450 
   Size:       1639    1621     190

  Fragments arranged by size: 

                1639    1621     190
 
AleI CACnn'nnGTG

Cuts at:      0     146    3450 
   Size:        146    3304
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2641    3450 
   Size:       2641     809
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     499     531     577     609    3450 
   Size:        499      32      46      32    2841

  Fragments arranged by size: 

                2841     499      46      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1027    3450 
   Size:       1027    2423
 
ApoI r'AATT_y

Cuts at:      0     376     883    1122    1348    1471    1503    1868    2398 
   Size:        376     507     239     226     123      32     365     530

Cuts at:   2398    2518    2716    3370    3450 
   Size:        120     198     654      80

  Fragments arranged by size: 

                 654     530     507     376     365     239     226     198
                 123     120      80      32
 
BanII G_rGCy'C

Cuts at:      0    1767    3044    3248    3450 
   Size:       1767    1277     204     202

  Fragments arranged by size: 

                1767    1277     204     202
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     629    1454    1802    2326    2401    2912    3450 
   Size:        629     825     348     524      75     511     538

  Fragments arranged by size: 

                 825     629     538     524     511     348      75
 
BbvCI CC'TCA_GC

Cuts at:      0    3272    3450 
   Size:       3272     178
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     573    2472    3450 
   Size:        573    1899     978

  Fragments arranged by size: 

                1899     978     573
 
BclI T'GATC_A

Cuts at:      0     929    1988    2736    3450 
   Size:        929    1059     748     714

  Fragments arranged by size: 

                1059     929     748     714
 
BfrBI ATG'CAT

Cuts at:      0     758    3450 
   Size:        758    2692
 
BglII A'GATC_T

Cuts at:      0     198    1656    2575    3120    3450 
   Size:        198    1458     919     545     330

  Fragments arranged by size: 

                1458     919     545     330     198
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     964    3264    3450 
   Size:        964    2300     186

  Fragments arranged by size: 

                2300     964     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1964    2024    2498    3010    3193    3450 
   Size:       1964      60     474     512     183     257

  Fragments arranged by size: 

                1964     512     474     257     183      60
 
Bpu10I CC'TnA_GC

Cuts at:      0    3242    3272    3450 
   Size:       3242      30     178

  Fragments arranged by size: 

                3242     178      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1681    1790    3450 
   Size:       1681     109    1660

  Fragments arranged by size: 

                1681    1660     109
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1478    1564    2497    3450 
   Size:       1478      86     933     953

  Fragments arranged by size: 

                1478     953     933      86
 
BsaBI GATnn'nnATC

Cuts at:      0     197    2735    3450 
   Size:        197    2538     715

  Fragments arranged by size: 

                2538     715     197
 
BsaWI w'CCGG_w

Cuts at:      0    1639    3260    3450 
   Size:       1639    1621     190

  Fragments arranged by size: 

                1639    1621     190
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2954    2984    3450 
   Size:       2954      30     466

  Fragments arranged by size: 

                2954     466      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    3019    3289    3450 
   Size:       3019     270     161

  Fragments arranged by size: 

                3019     270     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    2846    3153    3450 
   Size:        176    2670     307     297

  Fragments arranged by size: 

                2670     307     297     176
 
BsmI GAATG_Cn'

Cuts at:      0    1825    2109    3450 
   Size:       1825     284    1341

  Fragments arranged by size: 

                1825    1341     284
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2957    3277    3450 
   Size:       2957     320     173

  Fragments arranged by size: 

                2957     320     173
 
Bsp1286I G_dGCh'C

Cuts at:      0    1767    3044    3248    3450 
   Size:       1767    1277     204     202

  Fragments arranged by size: 

                1767    1277     204     202
 
BspHI T'CATG_A

Cuts at:      0    1314    2172    2382    3450 
   Size:       1314     858     210    1068

  Fragments arranged by size: 

                1314    1068     858     210
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     109    3363    3450 
   Size:        109    3254      87

  Fragments arranged by size: 

                3254     109      87
 
BsrDI GCAATG_nn'

Cuts at:      0    2348    3450 
   Size:       2348    1102
 
BsrFI r'CCGG_y

Cuts at:      0      62    1639    3260    3450 
   Size:         62    1577    1621     190

  Fragments arranged by size: 

                1621    1577     190      62
 
BsrGI T'GTAC_A

Cuts at:      0    1095    3450 
   Size:       1095    2355
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     229    3450 
   Size:        229    3221
 
BstYI r'GATC_y

Cuts at:      0     198     298    1656    2575    3120    3201    3450 
   Size:        198     100    1358     919     545      81     249

  Fragments arranged by size: 

                1358     919     545     249     198     100      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1442    3450 
   Size:       1442    2008
 
BtgI C'CryG_G

Cuts at:      0    2128    2540    3029    3450 
   Size:       2128     412     489     421

  Fragments arranged by size: 

                2128     489     421     412
 
BtsI GCAGTG_nn'

Cuts at:      0    3016    3450 
   Size:       3016     434
 
DraI TTT'AAA

Cuts at:      0     728    1619    3450 
   Size:        728     891    1831

  Fragments arranged by size: 

                1831     891     728
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2340    3450 
   Size:       2340    1110
 
EaeI y'GGCC_r

Cuts at:      0     486    3450 
   Size:        486    2964
 
EarI CTCTTCn'nnn_

Cuts at:      0    1250    1472    1514    1686    1748    2645    3121    3450 
   Size:       1250     222      42     172      62     897     476     329

  Fragments arranged by size: 

                1250     897     476     329     222     172      62      42
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     638    1940    1964    2024    2165    2449    2498 
   Size:        266     372    1302      24      60     141     284      49

Cuts at:   2498    2921    3010    3145    3193    3450 
   Size:        423      89     135      48     257

  Fragments arranged by size: 

                1302     423     372     284     266     257     141     135
                  89      60      49      48      24
 
EcoRI G'AATT_C

Cuts at:      0     883    2518    3450 
   Size:        883    1635     932

  Fragments arranged by size: 

                1635     932     883
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     711    3450 
   Size:        711    2739
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     332     364    1441    1473    1534    1566 
   Size:        190      32     110      32    1077      32      61      32

Cuts at:   1566    1654    1686    2377    2409    2668    2700    3450 
   Size:         88      32     691      32     259      32     750

  Fragments arranged by size: 

                1077     750     691     259     190     110      88      61
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1637    2571    3450 
   Size:       1637     934     879

  Fragments arranged by size: 

                1637     934     879
 
HindIII A'AGCT_T

Cuts at:      0    3427    3450 
   Size:       3427      23
 
MscI TGG'CCA

Cuts at:      0     488    3450 
   Size:        488    2962
 
MslI CAynn'nnrTG

Cuts at:      0     146     175     227     688    1390    2353    2384    2387 
   Size:        146      29      52     461     702     963      31       3

Cuts at:   2387    2699    2953    3450 
   Size:        312     254     497

  Fragments arranged by size: 

                 963     702     497     461     312     254     146      52
                  31      29       3
 
MspA1I CmG'CkG

Cuts at:      0    2945    3094    3271    3450 
   Size:       2945     149     177     179

  Fragments arranged by size: 

                2945     179     177     149
 
NcoI C'CATG_G

Cuts at:      0    3029    3450 
   Size:       3029     421
 
NsiI A_TGCA'T

Cuts at:      0     760    3450 
   Size:        760    2690
 
NspI r_CATG'y

Cuts at:      0    1363    3450 
   Size:       1363    2087
 
PasI CC'CwG_GG

Cuts at:      0     284    3450 
   Size:        284    3166
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     520     976    3450 
   Size:        520     456    2474

  Fragments arranged by size: 

                2474     520     456
 
PfoI T'CCnGG_A

Cuts at:      0    1781    3320    3450 
   Size:       1781    1539     130

  Fragments arranged by size: 

                1781    1539     130
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2923    2955    3450 
   Size:       2923      32     495

  Fragments arranged by size: 

                2923     495      32
 
PsiI TTA'TAA

Cuts at:      0     494    3450 
   Size:        494    2956
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      65      97    3450 
   Size:         65      32    3353

  Fragments arranged by size: 

                3353      65      32
 
PstI C_TGCA'G

Cuts at:      0    2310    2431    3450 
   Size:       2310     121    1019

  Fragments arranged by size: 

                2310    1019     121
 
PvuII CAG'CTG

Cuts at:      0    3094    3450 
   Size:       3094     356
 
ScaI AGT'ACT

Cuts at:      0      30    2995    3450 
   Size:         30    2965     455

  Fragments arranged by size: 

                2965     455      30
 
SexAI A'CCwGG_T

Cuts at:      0     847    3450 
   Size:        847    2603
 
SfcI C'TryA_G

Cuts at:      0      42    1873    1917    2163    2306    2427    3450 
   Size:         42    1831      44     246     143     121    1023

  Fragments arranged by size: 

                1831    1023     246     143     121      44      42
 
SmlI C'TyrA_G

Cuts at:      0    1660    1805    3450 
   Size:       1660     145    1645

  Fragments arranged by size: 

                1660    1645     145
 
SpeI A'CTAG_T

Cuts at:      0    1812    3450 
   Size:       1812    1638
 
SspI AAT'ATT

Cuts at:      0    2558    3395    3450 
   Size:       2558     837      55

  Fragments arranged by size: 

                2558     837      55
 
StuI AGG'CCT

Cuts at:      0     268    3450 
   Size:        268    3182
 
StyI C'CwwG_G

Cuts at:      0    3029    3450 
   Size:       3029     421
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1701    3450 
   Size:       1701    1749
 
TatI w'GTAC_w

Cuts at:      0      28    1095    2970    2993    3450 
   Size:         28    1067    1875      23     457

  Fragments arranged by size: 

                1875    1067     457      28      23
 
Tth111I GACn'n_nGTC

Cuts at:      0    1310    3450 
   Size:       1310    2140
 
XbaI T'CTAG_A

Cuts at:      0     912    2436    3450 
   Size:        912    1524    1014

  Fragments arranged by size: 

                1524    1014     912
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2985    3450 
   Size:       2985     465
 

 Enzymes that do cut:

     AcuI     AgeI     AleI     AlfI     AloI    AlwNI     ApoI    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI     DrdI     EaeI
     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII  HindIII     MscI
     MslI   MspA1I     NcoI     NsiI     NspI     PasI    PflMI     PfoI
     PpiI     PsiI     PsrI     PstI    PvuII     ScaI    SexAI     SfcI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI  Tth111I
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BcgI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaAI    BsaHI    BseYI    BsiEI  BsiHKAI
    BsiWI    BspEI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI   DraIII     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI
    PpuMI    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     TstI     XhoI     XmaI
     XmnI     ZraI
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