Restriction map for pF1KB3031
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraII       DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StyI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII           8  CGCG                 CG^CG
AcyI         1141  GGCGTC               G[GA]^CG[CT]C
AhdI          484  GACGGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         523  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1767  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         484  GACGGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1049  CCCGAG               C^[CT]CG[GA]G
AviII         452  TGCGCA               TGC^GCA
BanII        1671  GAGCCC               G[GA]GC[CT]^C
BbsI         1720  GAAGAC               GAAGAC(2/6)
BclI          855  TGATCA               T^GATCA
BglI          214  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         573  AGATCT               A^GATCT
BpuAI        1720  GAAGAC               GAAGAC(2/6)
BsaBI         880  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1141  GGCGTC               G[GA]^CG[CT]C
BsiEI        1361  CGGTCG               CG[GA][CT]^CG
BsmFI        1399  GGGAC                GGGAC(10/14)
BsoBI        1049  CCCGAG               C^[CT]CG[GA]G
BsrBI        1175  CCGCTC               CCGCTC(-3/-3)
BsrFI        1359  GCCGGT               [GA]^CCGG[CT]
BstUI           8  CGCG                 CG^CG
Cfr10I       1359  GCCGGT               [GA]^CCGG[CT]
DsaI         1275  CCACGG               C^C[GA][CT]GG
Eam1105I      484  GACGGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1591  GAAGAG               CTCTTC(1/4)
FauI         1680  CCCGC                CCCGC(4/6)
FbaI          855  TGATCA               T^GATCA
FspI          452  TGCGCA               TGC^GCA
HaeII        1171  AGCGCC               [GA]GCGC^[CT]
HgaI         1142  GCGTC                GACGC(5/10)
Hin1I        1141  GGCGTC               G[GA]^CG[CT]C
Ksp632I      1591  GAAGAG               CTCTTC(1/4)
MamI          880  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI            8  CGCG                 CG^CG
PstI         1374  CTGCAG               CTGCA^G
SapI         1591  GAAGAGC              GCTCTTC(1/4)
ScaI          624  AGTACT               AGT^ACT
SmlI          634  CTCAAG               C^T[CT][GA]AG
SspI         1630  AATATT               AAT^ATT
StuI           94  AGGCCT               AGG^CCT
TaqI          690  TCGA                 T^CGA
TthHB8I       690  TCGA                 T^CGA
XcmI          420  CCAGAGCCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1767  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I       1324  GTGCAC               G^TGCAC
             1369  GTGCAC              
AlwI          316  GATCC                GGATC(4/5)
              886  GATCC               
ApaLI        1324  GTGCAC               G^TGCAC
             1369  GTGCAC              
AspHI        1324  GTGCAC               G[AT]GC[AT]^C
             1369  GTGCAC              
BsaI          712  GAGACC               GGTCTC(1/5)
             1493  GGTCTC              
BsgI          311  GTGCAG               GTGCAG(16/14)
             1706  GTGCAG              
BsiHKAI      1324  GTGCAC               G[AT]GC[AT]^C
             1369  GTGCAC              
BspHI        1061  TCATGA               T^CATGA
             1179  TCATGA              
BsrDI         981  CATTGC               GCAATG(2/0)
              479  GCAATG              
BstXI        1478  CCATCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1653  CCAGACGAGTGG        
BstYI         315  AGATCC               [GA]^GATC[CT]
              573  AGATCT              
DraIII       1276  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1700  CACCCTGTG           
EaeI         1267  TGGCCG               [CT]^GGCC[GA]
             1389  TGGCCG              
EcoNI         323  CCTCTGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1314  CCTGGAGGAGG         
HincII         60  GTTGAC               GT[CT]^[GA]AC
             1450  GTTGAC              
HindII         60  GTTGAC               GT[CT]^[GA]AC
             1450  GTTGAC              
MflI          315  AGATCC               [GA]^GATC[CT]
              573  AGATCT              
PleI          654  GACTC                GAGTC(4/5)
             1733  GAGTC               
RcaI         1061  TCATGA               T^CATGA
             1179  TCATGA              
Tsp45I        151  GTCAC                ^GT[GC]AC
              499  GTGAC               
XhoII         315  AGATCC               [GA]^GATC[CT]
              573  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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