################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 428 CGCG CG^CG AvaI 85 CCCGAG C^[CT]CG[GA]G AvaII 950 GGTCC G^G[AT]CC BalI 1097 TGGCCA TGG^CCA BbsI 113 GAAGAC GAAGAC(2/6) BpuAI 113 GAAGAC GAAGAC(2/6) BseRI 1345 GAGGAG GAGGAG(10/8) BsiYI 272 CCAAAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 272 CCAAAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 85 CCCGAG C^[CT]CG[GA]G BsrBI 19 GAGCGG CCGCTC(-3/-3) BsrI 1280 ACTGG ACTGG(1/-1) Bst1107I 764 GTATAC GTA^TAC BstBI 599 TTCGAA TT^CGAA BstEII 390 GGTTACC G^GT[ACGT]ACC BstPI 390 GGTTACC G^GT[ACGT]ACC BstUI 428 CGCG CG^CG BstZ17I 764 GTATAC GTA^TAC Cfr13I 950 GGTCC G^G[ACGT]CC DraI 915 TTTAAA TTT^AAA DsaI 65 CCACGG C^C[GA][CT]GG EaeI 1097 TGGCCA [CT]^GGCC[GA] EarI 801 GAAGAG CTCTTC(1/4) EcoO65I 390 GGTTACC G^GT[ACGT]ACC FauI 21 GCGGG CCCGC(4/6) HaeIII 1098 GGCC GG^CC HindIII 919 AAGCTT A^AGCTT Ksp632I 801 GAAGAG CTCTTC(1/4) MluNI 1097 TGGCCA TGG^CCA MscI 1097 TGGCCA TGG^CCA MspA1I 694 CAGCTG C[AC]G^C[GT]G MvnI 428 CGCG CG^CG NheI 99 GCTAGC G^CTAGC NlaIII 706 CATG CATG^ NspV 599 TTCGAA TT^CGAA PvuII 694 CAGCTG CAG^CTG Sau96I 950 GGTCC G^G[ACGT]CC ScaI 674 AGTACT AGT^ACT SfuI 599 TTCGAA TT^CGAA SmlI 997 CTTGAG C^T[CT][GA]AG TfiI 690 GATTC G^A[AT]TC Tsp45I 875 GTGAC ^GT[GC]AC TspRI 319 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 764 GTATAC GT^[AC][GT]AC 1216 GTAGAC AseI 369 ATTAAT AT^TAAT 1302 ATTAAT AsnI 369 ATTAAT AT^TAAT 1302 ATTAAT BamHI 712 GGATCC G^GATCC 1257 GGATCC BbvI 484 GCAGC GCAGC(8/12) 789 GCAGC BsaI 1248 GAGACC GGTCTC(1/5) 1283 GGTCTC BssKI 642 CCAGG ^CC[ACGT]GG 825 CCTGG BstNI 642 CCAGG CC^[AT]GG 825 CCTGG DdeI 198 CTTAG C^T[ACGT]AG 1286 CTCAG EcoRII 642 CCAGG ^CC[AT]GG 825 CCTGG EcoT14I 104 CCAAGG C^C[AT][AT]GG 1253 CCAAGG HaeII 61 AGCGCC [GA]GCGC^[CT] 77 AGCGCC HincII 1192 GTTAAC GT[CT]^[GA]AC 1315 GTTAAC HindII 1192 GTTAAC GT[CT]^[GA]AC 1315 GTTAAC HpaI 1192 GTTAAC GTT^AAC 1315 GTTAAC MvaI 642 CCAGG CC^[AT]GG 825 CCTGG MwoI 94 GCGAAGCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 564 GCAAATGAGGC PshBI 369 ATTAAT AT^TAAT 1302 ATTAAT ScrFI 642 CCAGG CC^[ACGT]GG 825 CCTGG SspI 256 AATATT AAT^ATT 1084 AATATT StyI 104 CCAAGG C^C[AT][AT]GG 1253 CCAAGG TseI 484 GCAGC G^C[AT]GC 789 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I BfaI BsaJI BsmAI BstF5I BstYI Cac8I CfoI DpnII Fnu4HI FokI HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIV PleI RsaI Sau3AI SfaNI SfcI TaqI Tru9I Tsp509I TthHB8I XhoII XmnI