Restriction map for pF1KB3004
 (Linear) (Six-base) MAPSORT of: KIBB3004.seq  Check: 14  from: 1  to: 3150



KIBB3004.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0    2852    3150 
   Size:       2852     298
 
AclI AA'CG_TT

Cuts at:      0     298    2439    3150 
   Size:        298    2141     711

  Fragments arranged by size: 

                2141     711     298
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     148    1271    1770    2123    2212    2515    3150 
   Size:        106      42    1123     499     353      89     303     635

  Fragments arranged by size: 

                1123     635     499     353     303     106      89      42
            
 
AleI CACnn'nnGTG

Cuts at:      0    2163    3150 
   Size:       2163     987
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2190    2805    3028    3150 
   Size:       2190     615     223     122

  Fragments arranged by size: 

                2190     615     223     122
 
ApoI r'AATT_y

Cuts at:      0     194    1225    1241    1738    2105    2983    3150 
   Size:        194    1031      16     497     367     878     167

  Fragments arranged by size: 

                1031     878     497     367     194     167      16
 
AvaI C'yCGr_G

Cuts at:      0     593    3150 
   Size:        593    2557
 
BanI G'GyrC_C

Cuts at:      0    2349    2504    3150 
   Size:       2349     155     646

  Fragments arranged by size: 

                2349     646     155
 
BanII G_rGCy'C

Cuts at:      0     720    2011    3150 
   Size:        720    1291    1139

  Fragments arranged by size: 

                1291    1139     720
 
BbeI G_GCGC'C

Cuts at:      0    2353    3150 
   Size:       2353     797
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     647    1671    1823    2027    2092    2243    2904    3150 
   Size:        647    1024     152     204      65     151     661     246

  Fragments arranged by size: 

                1024     661     647     246     204     152     151      65
            
 
BbvCI CC'TCA_GC

Cuts at:      0    2779    3150 
   Size:       2779     371
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1863    3150 
   Size:       1863    1287
 
BclI T'GATC_A

Cuts at:      0    1395    2457    3150 
   Size:       1395    1062     693

  Fragments arranged by size: 

                1395    1062     693
 
BglII A'GATC_T

Cuts at:      0    1926    3150 
   Size:       1926    1224
 
BlpI GC'TnA_GC

Cuts at:      0    2005    3150 
   Size:       2005    1145
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1007    1565    3150 
   Size:       1007     558    1585

  Fragments arranged by size: 

                1585    1007     558
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     760    3150 
   Size:        760    2390
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    1706    3150 
   Size:         70    1636    1444

  Fragments arranged by size: 

                1636    1444      70
 
Bpu10I CC'TnA_GC

Cuts at:      0    1096    2779    3150 
   Size:       1096    1683     371

  Fragments arranged by size: 

                1683    1096     371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     643     730    1779    2410    2667    3150 
   Size:        254     389      87    1049     631     257     483

  Fragments arranged by size: 

                1049     631     483     389     257     254      87
 
BsaBI GATnn'nnATC

Cuts at:      0    2456    3150 
   Size:       2456     694
 
BsaHI Gr'CG_yC

Cuts at:      0    2350    3150 
   Size:       2350     800
 
BsaWI w'CCGG_w

Cuts at:      0    1259    1917    3150 
   Size:       1259     658    1233

  Fragments arranged by size: 

                1259    1233     658
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     652    1183    2034    2076    3150 
   Size:        652     531     851      42    1074

  Fragments arranged by size: 

                1074     851     652     531      42
 
BseYI C'CCAG_C

Cuts at:      0     572    3150 
   Size:        572    2578
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1025    1325    2779    3150 
   Size:       1025     300    1454     371

  Fragments arranged by size: 

                1454    1025     371     300
 
BsiHKAI G_wGCw'C

Cuts at:      0      30     132     720     744    3150 
   Size:         30     102     588      24    2406

  Fragments arranged by size: 

                2406     588     102      30      24
 
BsmI GAATG_Cn'

Cuts at:      0    3009    3150 
   Size:       3009     141
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1402    3150 
   Size:       1402    1748
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     132     720     744    2011    3150 
   Size:         30     102     588      24    1267    1139

  Fragments arranged by size: 

                1267    1139     588     102      30      24
 
BspEI T'CCGG_A

Cuts at:      0    1259    3150 
   Size:       1259    1891
 
BspHI T'CATG_A

Cuts at:      0    1866    3150 
   Size:       1866    1284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     938    3150 
   Size:        938    2212
 
BsrDI GCAATG_nn'

Cuts at:      0     402    3150 
   Size:        402    2748
 
BsrGI T'GTAC_A

Cuts at:      0      84     413    2796    3150 
   Size:         84     329    2383     354

  Fragments arranged by size: 

                2383     354     329      84
 
BssSI C'ACGA_G

Cuts at:      0     968    1646    3150 
   Size:        968     678    1504

  Fragments arranged by size: 

                1504     968     678
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     439    3150 
   Size:         48     391    2711

  Fragments arranged by size: 

                2711     391      48
 
BstYI r'GATC_y

Cuts at:      0    1128    1638    1887    1926    2535    2801    2819    3150 
   Size:       1128     510     249      39     609     266      18     331

  Fragments arranged by size: 

                1128     609     510     331     266     249      39      18
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2752    3150 
   Size:       2752     398
 
BtgI C'CryG_G

Cuts at:      0     880    2161    2404    2637    3150 
   Size:        880    1281     243     233     513

  Fragments arranged by size: 

                1281     880     513     243     233
 
DraI TTT'AAA

Cuts at:      0    2444    3150 
   Size:       2444     706
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     629    1028    1306    1334    1400    3150 
   Size:        167     462     399     278      28      66    1750

  Fragments arranged by size: 

                1750     462     399     278     167      66      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     120    3150 
   Size:        120    3030
 
EcoICRI GAG'CTC

Cuts at:      0     718    3150 
   Size:        718    2432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     106     148    1271    1706    1770    2123    2212 
   Size:         70      36      42    1123     435      64     353      89

Cuts at:   2212    2515    3150 
   Size:        303     635

  Fragments arranged by size: 

                1123     635     435     353     303      89      70      64
                  42      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     931     950    1567    2924    3150 
   Size:        931      19     617    1357     226

  Fragments arranged by size: 

                1357     931     617     226      19
 
EcoRI G'AATT_C

Cuts at:      0    1225    3150 
   Size:       1225    1925
 
HaeII r_GCGC'y

Cuts at:      0    2353    3150 
   Size:       2353     797
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     707     739    1861    1893    2804    2836    3150 
   Size:        707      32    1122      32     911      32     314

  Fragments arranged by size: 

                1122     911     707     314      32      32      32
 
HincII GTy'rAC

Cuts at:      0     296    2748    3122    3150 
   Size:        296    2452     374      28

  Fragments arranged by size: 

                2452     374     296      28
 
HindIII A'AGCT_T

Cuts at:      0    2714    3150 
   Size:       2714     436
 
HpaI GTT'AAC

Cuts at:      0    3122    3150 
   Size:       3122      28
 
KasI G'GCGC_C

Cuts at:      0    2349    3150 
   Size:       2349     801
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2237    2568    3150 
   Size:       2237     331     582

  Fragments arranged by size: 

                2237     582     331
 
MslI CAynn'nnrTG

Cuts at:      0    2163    3150 
   Size:       2163     987
 
MspA1I CmG'CkG

Cuts at:      0     248    1003    2471    2763    2783    3150 
   Size:        248     755    1468     292      20     367

  Fragments arranged by size: 

                1468     755     367     292     248      20
 
NarI GG'CG_CC

Cuts at:      0    2350    3150 
   Size:       2350     800
 
NcoI C'CATG_G

Cuts at:      0     880    2161    2404    2637    3150 
   Size:        880    1281     243     233     513

  Fragments arranged by size: 

                1281     880     513     243     233
 
NdeI CA'TA_TG

Cuts at:      0    1026    3150 
   Size:       1026    2124
 
PasI CC'CwG_GG

Cuts at:      0     902    3150 
   Size:        902    2248
 
PfoI T'CCnGG_A

Cuts at:      0    2804    3150 
   Size:       2804     346
 
PpuMI rG'GwC_Cy

Cuts at:      0     950    1567    2924    3150 
   Size:        950     617    1357     226

  Fragments arranged by size: 

                1357     950     617     226
 
PshAI GACnn'nnGTC

Cuts at:      0     567    2648    3150 
   Size:        567    2081     502

  Fragments arranged by size: 

                2081     567     502
 
PsiI TTA'TAA

Cuts at:      0    2591    3150 
   Size:       2591     559
 
PspXI vC'TCGA_Gb

Cuts at:      0     593    3150 
   Size:        593    2557
 
PstI C_TGCA'G

Cuts at:      0    1638    2223    2367    3150 
   Size:       1638     585     144     783

  Fragments arranged by size: 

                1638     783     585     144
 
PvuII CAG'CTG

Cuts at:      0     248    2471    2763    3150 
   Size:        248    2223     292     387

  Fragments arranged by size: 

                2223     387     292     248
 
SacI G_AGCT'C

Cuts at:      0     720    3150 
   Size:        720    2430
 
SexAI A'CCwGG_T

Cuts at:      0    2846    3150 
   Size:       2846     304
 
SfcI C'TryA_G

Cuts at:      0     314     565     848     869    1634    1977    2219    2363 
   Size:        314     251     283      21     765     343     242     144

Cuts at:   2363    2739    2905    3150 
   Size:        376     166     245

  Fragments arranged by size: 

                 765     376     343     314     283     251     245     242
                 166     144      21
 
SfoI GGC'GCC

Cuts at:      0    2351    3150 
   Size:       2351     799
 
SmlI C'TyrA_G

Cuts at:      0     269     593     622     745    1758    2425    2646    3150 
   Size:        269     324      29     123    1013     667     221     504

  Fragments arranged by size: 

                1013     667     504     324     269     221     123      29
            
 
SpeI A'CTAG_T

Cuts at:      0    3124    3150 
   Size:       3124      26
 
SspI AAT'ATT

Cuts at:      0    2949    3150 
   Size:       2949     201
 
StuI AGG'CCT

Cuts at:      0     966    3150 
   Size:        966    2184
 
StyI C'CwwG_G

Cuts at:      0     432     880     934     953    2161    2404    2637    2653 
   Size:        432     448      54      19    1208     243     233      16

Cuts at:   2653    3150 
   Size:        497

  Fragments arranged by size: 

                1208     497     448     432     243     233      54      19
                  16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2334    3150 
   Size:       2334     816
 
TatI w'GTAC_w

Cuts at:      0      84     413    2796    3150 
   Size:         84     329    2383     354

  Fragments arranged by size: 

                2383     354     329      84
 
XbaI T'CTAG_A

Cuts at:      0    1929    3150 
   Size:       1929    1221
 
XhoI C'TCGA_G

Cuts at:      0     593    3150 
   Size:        593    2557
 
XmnI GAAnn'nnTTC

Cuts at:      0     533    1241    2983    3150 
   Size:        533     708    1742     167

  Fragments arranged by size: 

                1742     708     533     167
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AleI    AlwNI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstXI    BstYI   Bsu36I
     BtgI     DraI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MmeI     MslI
   MspA1I     NarI     NcoI     NdeI     PasI     PfoI    PpuMI    PshAI
     PsiI    PspXI     PstI    PvuII     SacI    SexAI     SfcI     SfoI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     XbaI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BfrBI     BglI Bme1580I    BmgBI     BmtI
     BsaI    BsaAI    BsaXI    BsiEI    BsiWI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI     KpnI     MfeI     MluI     MscI     NaeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI   PspOMI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     TstI  Tth111I     XcmI
     XmaI     ZraI
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