Restriction map for pF1KB3002
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       AccII       AclI        AflII       AflIII      
AgeI        AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
BbrPI       BcgI        BclI        BfrI        BglII       
BsaAI       BsaBI       BsaI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
HgaI        HindIII     HpaI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1055  GGTACC               G^GTACC
AccI         1282  GTCGAC               GT^[AC][GT]AC
AccIII       1610  TCCGGA               T^CCGGA
AcsI           44  AAATTC               [GA]^AATT[CT]
AcyI         1460  GGCGCC               G[GA]^CG[CT]C
Alw44I        722  GTGCAC               G^TGCAC
AlwNI         692  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         722  GTGCAC               G^TGCAC
ApoI           44  AAATTC               [GA]^AATT[CT]
Asp718I      1055  GGTACC               G^GTACC
AviII         146  TGCGCA               TGC^GCA
AvrII          85  CCTAGG               C^CTAGG
BamHI           3  GGATCC               G^GATCC
BbeI         1460  GGCGCC               GGCGC^C
BbvCI        1593  CCTCAGC              CCTCAGC(-5/-2)
BciVI         902  GTATCC               GTATCC(6/5)
BglI         1443  GCCAGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           85  CCTAGG               C^CTAGG
BpmI          949  CTGGAG               CTGGAG(16/14)
BsaHI        1460  GGCGCC               G[GA]^CG[CT]C
BsaWI        1610  TCCGGA               [AT]^CCGG[AT]
BseAI        1610  TCCGGA               T^CCGGA
BspEI        1610  TCCGGA               T^CCGGA
BspMI        1707  ACCTGC               ACCTGC(4/8)
DraIII        244  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1620  CCATGG               C^C[GA][CT]GG
EcoT22I        56  ATGCAT               ATGCA^T
FspI          146  TGCGCA               TGC^GCA
HaeII        1460  GGCGCC               [GA]GCGC^[CT]
Hin1I        1460  GGCGCC               G[GA]^CG[CT]C
HincII       1282  GTCGAC               GT[CT]^[GA]AC
HindII       1282  GTCGAC               GT[CT]^[GA]AC
KasI         1460  GGCGCC               G^GCGCC
KpnI         1055  GGTACC               GGTAC^C
MroI         1610  TCCGGA               T^CCGGA
MseI         1217  TTAA                 T^TAA
NaeI         1430  GCCGGC               GCC^GGC
NarI         1460  GGCGCC               GG^CGCC
NcoI         1620  CCATGG               C^CATGG
NgoMIV       1430  GCCGGC               G^CCGGC
NsiI           56  ATGCAT               ATGCA^T
PflMI        1016  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1575  GAGTC                GAGTC(4/5)
PstI          897  CTGCAG               CTGCA^G
SalI         1282  GTCGAC               G^TCGAC
SapI         1008  GCTCTTC              GCTCTTC(1/4)
SfoI         1460  GGCGCC               GGC^GCC
SgrAI        1456  CACCGGCG             C[GA]^CCGG[CT]G
TaqI         1283  TCGA                 T^CGA
Tru9I        1217  TTAA                 T^TAA
Tsp509I        45  AATT                 ^AATT
TthHB8I      1283  TCGA                 T^CGA
Van91I       1016  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          167  CCCGGG               C^[CT]CG[GA]G
              312  CCCGGG              
BfaI           86  CTAG                 C^TAG
              686  CTAG                
BsgI         1526  CTGCAC               GTGCAG(16/14)
              620  GTGCAG              
BsmAI         812  GAGAC                GTCTC(1/5)
              528  GTCTC               
BsoBI         167  CCCGGG               C^[CT]CG[GA]G
              312  CCCGGG              
BstYI           3  GGATCC               [GA]^GATC[CT]
              944  AGATCC              
CfoI          147  GCGC                 GCG^C
             1461  GCGC                
FauI          956  GCGGG                CCCGC(4/6)
             1716  GCGGG               
HhaI          147  GCGC                 GCG^C
             1461  GCGC                
HinP1I        147  GCGC                 G^CGC
             1461  GCGC                
MaeI           86  CTAG                 C^TAG
              686  CTAG                
MaeII        1155  ACGT                 A^CGT
             1286  ACGT                
MflI            3  GGATCC               [GA]^GATC[CT]
              944  AGATCC              
NspI         1240  GCATGT               [GA]CATG^[CT]
             1587  ACATGC              
SacI          123  GAGCTC               GAGCT^C
             1006  GAGCTC              
ScaI           69  AGTACT               AGT^ACT
             1415  AGTACT              
SmaI          167  CCCGGG               CCC^GGG
              312  CCCGGG              
TfiI           81  GATTC                G^A[AT]TC
              386  GATTC               
XhoII           3  GGATCC               [GA]^GATC[CT]
              944  AGATCC              
XmaCI         167  CCCGGG               C^CCGGG
              312  CCCGGG              
XmaI          167  CCCGGG               C^CCGGG
              312  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CelII       Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TseI        Tsp45I      TspRI       XcmI        
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