Restriction map for pF1KB1487
##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BbeI        
BfrI        BlnI        BsaHI       BseAI       BsiEI       
BspDI       BspEI       BssSI       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       EcoT22I     FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NotI        NsiI        PacI        PaeR7I      PinAI       
PmeI        PshBI       PvuI        RsrII       SacII       
SalI        ScaI        SexAI       SfoI        SgrAI       
SmaI        SnaBI       SpeI        SspI        SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          309  GTATAC               GT^[AC][GT]AC
AclI         5014  AACGTT               AA^CGTT
Aor51HI      2098  AGCGCT               AGC^GCT
ApaI         3061  GGGCCC               GGGCC^C
Asp700I      4476  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI          57  GGATCC               G^GATCC
BbrPI         693  CACGTG               CAC^GTG
BcgI          535  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         4895  TGATCA               T^GATCA
BlpI         1696  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1696  GCTGAGC              GC^T[ACGT]AGC
BsiWI        1608  CGTACG               C^GTACG
BspHI        4535  TCATGA               T^CATGA
BspLU11I     4928  ACATGT               A^CATGT
BsrBI        5338  CCGCTC               CCGCTC(-3/-3)
BssHII       3898  GCGCGC               G^CGCGC
Bst1107I      309  GTATAC               GTA^TAC
BstBI         154  TTCGAA               TT^CGAA
BstZ17I       309  GTATAC               GTA^TAC
CelII        1696  GCTGAGC              GC^T[ACGT]AGC
Eco47III     2098  AGCGCT               AGC^GCT
FbaI         4895  TGATCA               T^GATCA
FseI          556  GGCCGGCC             GGCCGG^CC
HindIII      5378  AAGCTT               A^AGCTT
NdeI         4067  CATATG               CA^TATG
NheI         3184  GCTAGC               G^CTAGC
NruI         5442  TCGCGA               TCG^CGA
NspV          154  TTCGAA               TT^CGAA
PmaCI         693  CACGTG               CAC^GTG
PmlI          693  CACGTG               CAC^GTG
Psp1406I     5014  AACGTT               AA^CGTT
RcaI         4535  TCATGA               T^CATGA
SapI         4285  GAAGAGC              GCTCTTC(1/4)
SfiI         2549  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          154  TTCGAA               TT^CGAA
XmnI         4476  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspI         2808  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4044  GACCCTGTC           
AvaI         4333  CCCGAG               C^[CT]CG[GA]G
             5509  CCCGAG              
BciVI        5497  GGATAC               GTATCC(6/5)
             2042  GTATCC              
BsaBI        4406  GATTAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4527  GATTAGCATC          
BsmBI        3462  CGTCTC               CGTCTC(1/5)
             2632  GAGACG              
BsoBI        4333  CCCGAG               C^[CT]CG[GA]G
             5509  CCCGAG              
Bsp1407I      689  TGTACA               T^GTACA
             4086  TGTACA              
BsrGI         689  TGTACA               T^GTACA
             4086  TGTACA              
DraIII       4010  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5130  CACGCAGTG           
HaeII        1783  AGCGCC               [GA]GCGC^[CT]
             2098  AGCGCT              
HgaI         3393  GACGC                GACGC(5/10)
             3523  GACGC               
MamI         4406  GATTAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4527  GATTAGCATC          
MseI         3212  TTAA                 T^TAA
             5596  TTAA                
PflFI        2808  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4044  GACCCTGTC           
PshAI        1002  GACTCCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4813  GACTTTGGTC          
SbfI         1038  CCTGCAGG             CCTGCA^GG
             1875  CCTGCAGG            
SphI          641  GCATGC               GCATG^C
             2361  GCATGC              
Sse8387I     1038  CCTGCAGG             CCTGCA^GG
             1875  CCTGCAGG            
SspBI         689  TGTACA               T^GTACA
             4086  TGTACA              
Tru9I        3212  TTAA                 T^TAA
             5596  TTAA                
Tth111I      2808  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4044  GACCCTGTC           
XcmI          746  CCATCCAAGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4595  CCAACATCGTCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       ApoI        AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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