Restriction map for pF1KB1487
 (Circular) (Six-base) MAPSORT of: pF1KB1487.seq  Check: 421  from: 1  to: 8852



pF1KB1487  8852 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1795    1861    1869    2197    5755    5788    1795 
   Size:         66       8     328    3558      33    4859

  Fragments arranged by size: 

                4859    3558     328      66      33       8
 
AccI GT'mk_AC

Cuts at:    418    5890     418 
   Size:       5472    3380
 
Acc65I G'GTAC_C

Cuts at:   5868    5868 
   Size:       8852
 
AclI AA'CG_TT

Cuts at:   5123    8692    5123 
   Size:       3569    5283
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1105    1457    1687    2084    2404    2746    2872    3149 
   Size:        575     352     230     397     320     342     126     277

Cuts at:   3149    3232    3268    4412    4981    5018    5227    5233    5492 
   Size:         83      36    1144     569      37     209       6     259

Cuts at:   5492    6677    7109    7856    8124     530 
   Size:       1185     432     747     268    1258

  Fragments arranged by size: 

                1258    1185    1144     747     575     569     432     397
                 352     342     320     277     268     259     230     209
                 126      83      37      36       6
 
AfeI AGC'GCT

Cuts at:   2208    2208 
   Size:       8852
 
AflIII A'CryG_T

Cuts at:    800    2432    4331    5036    6204    7308    8285     800 
   Size:       1632    1899     705    1168    1104     977    1367

  Fragments arranged by size: 

                1899    1632    1367    1168    1104     977     705
 
AgeI A'CCGG_T

Cuts at:   7240    7240 
   Size:       8852
 
AleI CACnn'nnGTG

Cuts at:   1465    4087    1465 
   Size:       2622    6230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    734    3466     734 
   Size:       2732    6120
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    839     871    3271    3303     839 
   Size:         32    2400      32    6388

  Fragments arranged by size: 

                6388    2400      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    759    1895    3417    4287    6157    7724     759 
   Size:       1136    1522     870    1870    1567    1887

  Fragments arranged by size: 

                1887    1870    1567    1522    1136     870
 
ApaI G_GGCC'C

Cuts at:   3173    3173 
   Size:       8852
 
ApaLI G'TGCA_C

Cuts at:   2362    2941    4852    7622    2362 
   Size:        579    1911    2770    3592

  Fragments arranged by size: 

                3592    2770    1911     579
 
ApoI r'AATT_y

Cuts at:   1351    4528    4984    5856    8205    8461    1351 
   Size:       3177     456     872    2349     256    1742

  Fragments arranged by size: 

                3177    2349    1742     872     456     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       8852
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8852
 
AvaI C'yCGr_G

Cuts at:   4441    5617    5872    4441 
   Size:       1176     255    7421

  Fragments arranged by size: 

                7421    1176     255
 
BamHI G'GATC_C

Cuts at:    165    5877     165 
   Size:       5712    3140
 
BanI G'GyrC_C

Cuts at:   1459    1843    2260    3018    4900    4975    5868    6282    6530 
   Size:        384     417     758    1882      75     893     414     248

Cuts at:   6530    6565    1459 
   Size:         35    3746

  Fragments arranged by size: 

                3746    1882     893     758     417     414     384     248
                  75      35
 
BanII G_rGCy'C

Cuts at:    293    1811    2562    3173    3733    4144    4442    5018    5408 
   Size:       1518     751     611     560     411     298     576     390

Cuts at:   5408    5795    5866    6896     293 
   Size:        387      71    1030    2249

  Fragments arranged by size: 

                2249    1518    1030     751     611     576     560     411
                 390     387     298      71
 
BbeI G_GCGC'C

Cuts at:   6286    6534    6286 
   Size:        248    8604
 
BbsI GAAGACnn'nnnn_

Cuts at:   1132    2705    2849    3464    5382    1132 
   Size:       1573     144     615    1918    4602

  Fragments arranged by size: 

                4602    1918    1573     615     144
 
BbvCI CC'TCA_GC

Cuts at:   1049    5130    5507    1049 
   Size:       4081     377    4394

  Fragments arranged by size: 

                4394    4081     377
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    632     666     632 
   Size:         34    8818
 
BciVI GTATCCnnnnn_n'

Cuts at:   2161    5599    6750    7511    8852    2161 
   Size:       3438    1151     761    1341    2161

  Fragments arranged by size: 

                3438    2161    1341    1151     761
 
BclI T'GATC_A

Cuts at:   5003    6372    5003 
   Size:       1369    7483
 
BglI GCCn_nnn'nGGC

Cuts at:   1801    2664    3168    1801 
   Size:        863     504    7485

  Fragments arranged by size: 

                7485     863     504
 
BglII A'GATC_T

Cuts at:   4214    4811    5168    6367    8404    4214 
   Size:        597     357    1199    2037    4662

  Fragments arranged by size: 

                4662    2037    1199     597     357
 
BlpI GC'TnA_GC

Cuts at:   1805    5964    1805 
   Size:       4159    4693
 
Bme1580I G_kGCm'C

Cuts at:    586     723    1736    2182    2366    2945    3173    4856    4903 
   Size:        137    1013     446     184     579     228    1683      47

Cuts at:   4903    6477    6570    7626     586 
   Size:       1574      93    1056    1812

  Fragments arranged by size: 

                1812    1683    1574    1056    1013     579     446     228
                 184     137      93      47
 
BmgBI CAC'GTC

Cuts at:    789    2433    2712    4238     789 
   Size:       1644     279    1526    5403

  Fragments arranged by size: 

                5403    1644    1526     279
 
BmrI ACTGGGnnnn_n'

Cuts at:   1723    6064    6145    6480    8624    1723 
   Size:       4341      81     335    2144    1951

  Fragments arranged by size: 

                4341    2144    1951     335      81
 
BmtI G_CTAG'C

Cuts at:   3296    3296 
   Size:       8852
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1247    2150    7219    1247 
   Size:        903    5069    2880

  Fragments arranged by size: 

                5069    2880     903
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    984     991    1093    1229    1641    2231    3442    3662    4741 
   Size:          7     102     136     412     590    1211     220    1079

Cuts at:   4741    4963     984 
   Size:        222    4873

  Fragments arranged by size: 

                4873    1211    1079     590     412     222     220     136
                 102       7
 
Bpu10I CC'TnA_GC

Cuts at:   1049    3148    4664    5130    5507    1049 
   Size:       2099    1516     466     377    4394

  Fragments arranged by size: 

                4394    2099    1516     466     377
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     676    2479    3292    3505    4849    5515    6028    6907 
   Size:        520    1803     813     213    1344     666     513     879

Cuts at:   6907    7399    7697     156 
   Size:        492     298    1311

  Fragments arranged by size: 

                1803    1344    1311     879     813     666     520     513
                 492     298     213
 
BsaI GGTCTCn'nnnn_

Cuts at:    550    2795    3103    3199    3235    4961    8556     550 
   Size:       2245     308      96      36    1726    3595     846

  Fragments arranged by size: 

                3595    2245    1726     846     308      96      36
 
BsaAI yAC'GTr

Cuts at:    803    2183    4321    6205     803 
   Size:       1380    2138    1884    3450

  Fragments arranged by size: 

                3450    2138    1884    1380
 
BsaBI GATnn'nnATC

Cuts at:      6    4518    4639    6366    8403       6 
   Size:       4512     121    1727    2037     455

  Fragments arranged by size: 

                4512    2037    1727     455     121
 
BsaHI Gr'CG_yC

Cuts at:   6283    6531    7233    8729    6283 
   Size:        248     702    1496    6406

  Fragments arranged by size: 

                6406    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1113    4454    5309    6041    6562    7240    7514    7661    8072 
   Size:       3341     855     732     521     678     274     147     411

Cuts at:   8072    1113 
   Size:       1893

  Fragments arranged by size: 

                3341    1893     855     732     678     521     411     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1765    1795    3854    3884    4714    4744    4774    4804    1765 
   Size:         30    2059      30     830      30      30      30    5813

  Fragments arranged by size: 

                5813    2059     830      30      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    415    1223    1485    2624    2707    2773    4134    4161    4522 
   Size:        808     262    1139      83      66    1361      27     361

Cuts at:   4522     415 
   Size:       4745

  Fragments arranged by size: 

                4745    1361    1139     808     361     262      83      66
                  27
 
BseYI C'CCAG_C

Cuts at:    606    1957    2264    2862    2951    3060    3399    3483    4053 
   Size:       1351     307     598      89     109     339      84     570

Cuts at:   4053    4107    6277    7035    7612    8108     606 
   Size:         54    2170     758     577     496    1350

  Fragments arranged by size: 

                2170    1351    1350     758     598     577     570     496
                 339     307     109      89      84      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1551    1955    2062    2479    5220    5766    1551 
   Size:        404     107     417    2741     546    4637

  Fragments arranged by size: 

                4637    2741     546     417     404     107
 
BsiEI CG_ry'CG

Cuts at:    104    6394    6440    7648     104 
   Size:       6290      46    1208    1308

  Fragments arranged by size: 

                6290    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    450    1182    1714    1811    2237    2366    2945    3652    3733 
   Size:        732     532      97     426     129     579     707      81

Cuts at:   3733    3956    4019    4144    4856    5795    5866    6644    6834 
   Size:        223      63     125     712     939      71     778     190

Cuts at:   6834    7626     450 
   Size:        792    1676

  Fragments arranged by size: 

                1676     939     792     778     732     712     707     579
                 532     426     223     190     129     125      97      81
                  71      63
 
BsiWI C'GTAC_G

Cuts at:   1716    1716 
   Size:       8852
 
BsmI GAATG_Cn'

Cuts at:   1342    1872    2518    3123    4953    5158    1342 
   Size:        530     646     605    1830     205    5036

  Fragments arranged by size: 

                5036    1830     646     605     530     205
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2734    3576    2734 
   Size:        842    8010
 
Bsp1286I G_dGCh'C

Cuts at:    293     450     586     723    1182    1714    1736    1811    2182 
   Size:        157     136     137     459     532      22      75     371

Cuts at:   2182    2237    2366    2562    2945    3173    3652    3733    3956 
   Size:         55     129     196     383     228     479      81     223

Cuts at:   3956    4019    4144    4442    4856    4903    5018    5408    5795 
   Size:         63     125     298     414      47     115     390     387

Cuts at:   5795    5866    6477    6570    6644    6834    6896    7626     293 
   Size:         71     611      93      74     190      62     730    1519

  Fragments arranged by size: 

                1519     730     611     532     479     459     414     390
                 387     383     371     298     228     223     196     190
                 157     137     136     129     125     115      93      81
                  75      74      71      63      62      55      47      22
            
 
BspEI T'CCGG_A

Cuts at:   8072    8072 
   Size:       8852
 
BspHI T'CATG_A

Cuts at:   4643    8028    4643 
   Size:       3385    5467
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    669     744    1795    1861    1869    2197    2900    2946    3042 
   Size:         75    1051      66       8     328     703      46      96

Cuts at:   3042    3792    4295    5755    5788    5902    6116    6418    6799 
   Size:        750     503    1460      33     114     214     302     381

Cuts at:   6799     669 
   Size:       2722

  Fragments arranged by size: 

                2722    1460    1051     750     703     503     381     328
                 302     214     114      96      75      66      46      33
                   8
 
BsrBI CCG'CTC

Cuts at:     50    5448    7144    7198    8682    8847      50 
   Size:       5398    1696      54    1484     165      55

  Fragments arranged by size: 

                5398    1696    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3159    3267    3818    5361    5399    6764    3159 
   Size:        108     551    1543      38    1365    5247

  Fragments arranged by size: 

                5247    1543    1365     551     108      38
 
BsrFI r'CCGG_y

Cuts at:    665    1795    2473    6850    7031    7240     665 
   Size:       1130     678    4377     181     209    2277

  Fragments arranged by size: 

                4377    2277    1130     678     209     181
 
BsrGI T'GTAC_A

Cuts at:    797    4194     797 
   Size:       3397    5455
 
BssHII G'CGCG_C

Cuts at:   4006    4006 
   Size:       8852
 
BssSI C'ACGA_G

Cuts at:   7123    7481    7123 
   Size:        358    8494
 
BstAPI GCAn_nnn'nTGC

Cuts at:    995    3417    5243    6229     995 
   Size:       2422    1826     986    3618

  Fragments arranged by size: 

                3618    2422    1826     986
 
BstBI TT'CG_AA

Cuts at:    263    7213     263 
   Size:       6950    1902
 
BstEII G'GTnAC_C

Cuts at:    280     681     930    1899    2344    5432     280 
   Size:        401     249     969     445    3088    3700

  Fragments arranged by size: 

                3700    3088     969     445     401     249
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1015    3129    4941    5283    1015 
   Size:       2114    1812     342    4584

  Fragments arranged by size: 

                4584    2114    1812     342
 
BstYI r'GATC_y

Cuts at:      1     165    2093    2486    2858    3938    4214    4519    4811 
   Size:        164    1928     393     372    1080     276     305     292

Cuts at:   4811    5168    5877    6344    6367    6702    6948    7960    8046 
   Size:        357     709     467      23     335     246    1012      86

Cuts at:   8046    8058    8404       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1928    1080    1012     709     467     449     393     372
                 357     346     335     305     292     276     246     164
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    419     419 
   Size:       8852
 
Bsu36I CC'TnA_GG

Cuts at:    525    2653    3593    3839     525 
   Size:       2128     940     246    5538

  Fragments arranged by size: 

                5538    2128     940     246
 
BtgI C'CryG_G

Cuts at:    107     768    1319    1839    2566    3182    3254    3665    5257 
   Size:        661     551     520     727     616      72     411    1592

Cuts at:   5257     107 
   Size:       3702

  Fragments arranged by size: 

                3702    1592     727     661     616     551     520     411
                  72
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6802    6983    7146    6802 
   Size:        181     163    8508

  Fragments arranged by size: 

                8508     181     163
 
BtsI GCAGTG_nn'

Cuts at:    447     557     706    1568    1714    1967    3108    3134    3586 
   Size:        110     149     862     146     253    1141      26     452

Cuts at:   3586    4017    4033    4194    5248    6095     447 
   Size:        431      16     161    1054     847    3204

  Fragments arranged by size: 

                3204    1141    1054     862     847     452     431     253
                 161     149     146     110      26      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    423     458     423 
   Size:         35    8817
 
DraI TTT'AAA

Cuts at:   5851    5851 
   Size:       8852
 
DraIII CAC_nnn'GTG

Cuts at:   4123    5243    4123 
   Size:       1120    7732
 
DrdI GACnn_nn'nnGTC

Cuts at:   6558    7416    6558 
   Size:        858    7994
 
EaeI y'GGCC_r

Cuts at:    663    2403    2475    2665    2978    3636    4001    4931    5117 
   Size:       1740      72     190     313     658     365     930     186

Cuts at:   5117    6437    6611    7002    7029    8374     663 
   Size:       1320     174     391      27    1345    1141

  Fragments arranged by size: 

                1740    1345    1320    1141     930     658     391     365
                 313     190     186     174      72      27
 
EagI C'GGCC_G

Cuts at:   6437    6437 
   Size:       8852
 
EarI CTCTTCn'nnn_

Cuts at:   1208    1216    1912    1924    2722    2924    3740    4388    5828 
   Size:          8     696      12     798     202     816     648    1440

Cuts at:   5828    5837    6875    7085    1208 
   Size:          9    1038     210    2975

  Fragments arranged by size: 

                2975    1440    1038     816     798     696     648     210
                 202      12       9       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2758    3658    7060    7370    7516    8662    2758 
   Size:        900    3402     310     146    1146    2948

  Fragments arranged by size: 

                3402    2948    1146     900     310     146
 
EcoICRI GAG'CTC

Cuts at:   1809    3731    4142    5793    5864    1809 
   Size:       1922     411    1651      71    4797

  Fragments arranged by size: 

                4797    1922    1651     411      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    530     984     991    1093    1105    1229    1457    1641    1687 
   Size:        454       7     102      12     124     228     184      46

Cuts at:   1687    2084    2231    2404    2746    2872    3149    3232    3268 
   Size:        397     147     173     342     126     277      83      36

Cuts at:   3268    3442    3662    4412    4741    4963    4981    5018    5227 
   Size:        174     220     750     329     222      18      37     209

Cuts at:   5227    5233    5492    6677    7109    7856    8124     530 
   Size:          6     259    1185     432     747     268    1258

  Fragments arranged by size: 

                1258    1185     750     747     454     432     397     342
                 329     277     268     259     228     222     220     209
                 184     174     173     147     126     124     102      83
                  46      37      36      18      12       7       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:    196    1831    3547     196 
   Size:       1635    1716    5501

  Fragments arranged by size: 

                5501    1716    1635
 
EcoO109I rG'GnC_Cy

Cuts at:    201    1194    1724    1989    2595    2648    3169    3170    3699 
   Size:        993     530     265     606      53     521       1     529

Cuts at:   3699    4284    4965    5991     201 
   Size:        585     681    1026    3062

  Fragments arranged by size: 

                3062    1026     993     681     606     585     530     529
                 521     265      53       1
 
EcoRI G'AATT_C

Cuts at:   5856    5856 
   Size:       8852
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4986    5509    6871    4986 
   Size:        523    1362    6967

  Fragments arranged by size: 

                6967    1362     523
 
FseI GG_CCGG'CC

Cuts at:    669     669 
   Size:       8852
 
FspI TGC'GCA

Cuts at:   6633    6633 
   Size:       8852
 
HaeII r_GCGC'y

Cuts at:   1895    2210    6286    6534    7556    8535    1895 
   Size:        315    4076     248    1022     979    2212

  Fragments arranged by size: 

                4076    2212    1022     979     315     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    605     637    1495    1527    2953    2985    3037    3069    3697 
   Size:         32     858      32    1426      32      52      32     628

Cuts at:   3697    3729    3882    3914    4714    4746    5155    5187    5629 
   Size:         32     153      32     800      32     409      32     442

Cuts at:   5629    5661    6694    6724    6726    6756     605 
   Size:         32    1033      30       2      30    2701

  Fragments arranged by size: 

                2701    1426    1033     858     800     628     442     409
                 153      52      32      32      32      32      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    180     470     777    4077    4890    5760    5891     180 
   Size:        290     307    3300     813     870     131    3141

  Fragments arranged by size: 

                3300    3141     870     813     307     290     131
 
HindIII A'AGCT_T

Cuts at:   5486    5486 
   Size:       8852
 
KasI G'GCGC_C

Cuts at:   6282    6530    6282 
   Size:        248    8604
 
KpnI G_GTAC'C

Cuts at:   5872    5872 
   Size:       8852
 
MluI A'CGCG_T

Cuts at:   8285    8285 
   Size:       8852
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1601    5198    7523    7707    1601 
   Size:       3597    2325     184    2746

  Fragments arranged by size: 

                3597    2746    2325     184
 
MscI TGG'CCA

Cuts at:   2405    2667    2980    3638    4933    5119    6613    2405 
   Size:        262     313     658    1295     186    1494    4644

  Fragments arranged by size: 

                4644    1494    1295     658     313     262     186
 
MslI CAynn'nnrTG

Cuts at:   1465    3961    4087    4711    5035    6841    6968    1465 
   Size:       2496     126     624     324    1806     127    3349

  Fragments arranged by size: 

                3349    2496    1806     624     324     127     126
 
MspA1I CmG'CkG

Cuts at:    332     844    1565    2588    2790    2898    4111    5963    6277 
   Size:        512     721    1023     202     108    1213    1852     314

Cuts at:   6277    6637    7650    7895     332 
   Size:        360    1013     245    1289

  Fragments arranged by size: 

                1852    1289    1213    1023    1013     721     512     360
                 314     245     202     108
 
NaeI GCC'GGC

Cuts at:    667    1797    2475    7033     667 
   Size:       1130     678    4558    2486

  Fragments arranged by size: 

                4558    2486    1130     678
 
NarI GG'CG_CC

Cuts at:   6283    6531    6283 
   Size:        248    8604
 
NcoI C'CATG_G

Cuts at:    107     768    1319    1839    3254    5257     107 
   Size:        661     551     520    1415    2003    3702

  Fragments arranged by size: 

                3702    2003    1415     661     551     520
 
NdeI CA'TA_TG

Cuts at:   4176    4176 
   Size:       8852
 
NgoMIV G'CCGG_C

Cuts at:    665    1795    2473    7031     665 
   Size:       1130     678    4558    2486

  Fragments arranged by size: 

                4558    2486    1130     678
 
NheI G'CTAG_C

Cuts at:   3292    3292 
   Size:       8852
 
NruI TCG'CGA

Cuts at:   5552    5552 
   Size:       8852
 
NspI r_CATG'y

Cuts at:    753    2473    4259    4806    5040    5905    6405    7312     753 
   Size:       1720    1786     547     234     865     500     907    2293

  Fragments arranged by size: 

                2293    1786    1720     907     865     547     500     234
            
 
PasI CC'CwG_GG

Cuts at:   1028    2307    1028 
   Size:       1279    7573
 
PciI A'CATG_T

Cuts at:   5036    7308    5036 
   Size:       2272    6580
 
PflMI CCAn_nnn'nTGG

Cuts at:    864    3497    4680     864 
   Size:       2633    1183    5036

  Fragments arranged by size: 

                5036    2633    1183
 
PfoI T'CCnGG_A

Cuts at:   1420    1451    1817    2153    2165    3715    3941    4280    4718 
   Size:         31     366     336      12    1550     226     339     438

Cuts at:   4718    1420 
   Size:       5554

  Fragments arranged by size: 

                5554    1550     438     366     339     336     226      31
                  12
 
PmeI GTTT'AAAC

Cuts at:   5851    5851 
   Size:       8852
 
PmlI CAC'GTG

Cuts at:    803     803 
   Size:       8852
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2419    2451    7991    8023    2419 
   Size:         32    5540      32    3248

  Fragments arranged by size: 

                5540    3248      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1194    1724    2595    2648    4284    4965    1194 
   Size:        530     871      53    1636     681    5081

  Fragments arranged by size: 

                5081    1636     871     681     530      53
 
PshAI GACnn'nnGTC

Cuts at:   1114    4925    1114 
   Size:       3811    5041
 
PspOMI G'GGCC_C

Cuts at:   3169    3169 
   Size:       8852
 
PstI C_TGCA'G

Cuts at:    713     981    1151    1709    1891    1988    2897    5899    6584 
   Size:        268     170     558     182      97     909    3002     685

Cuts at:   6584     713 
   Size:       2981

  Fragments arranged by size: 

                3002    2981     909     685     558     268     182     170
                  97
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       8852
 
PvuII CAG'CTG

Cuts at:    844    1565    2588    2790    4111    6277    6637     844 
   Size:        721    1023     202    1321    2166     360    3059

  Fragments arranged by size: 

                3059    2166    1321    1023     721     360     202
 
RsrII CG'GwC_CG

Cuts at:   7047    7047 
   Size:       8852
 
SacI G_AGCT'C

Cuts at:   1811    3733    4144    5795    5866    1811 
   Size:       1922     411    1651      71    4797

  Fragments arranged by size: 

                4797    1922    1651     411      71
 
SalI G'TCGA_C

Cuts at:   5889    5889 
   Size:       8852
 
SanDI GG'GwC_CC

Cuts at:   1724    2648    1724 
   Size:        924    7928
 
SapI GCTCTTCn'nnn_

Cuts at:   4388    6875    7085    4388 
   Size:       2487     210    6155

  Fragments arranged by size: 

                6155    2487     210
 
SbfI CC_TGCA'GG

Cuts at:   1151    1988    5899    1151 
   Size:        837    3911    4104

  Fragments arranged by size: 

                4104    3911     837
 
ScaI AGT'ACT

Cuts at:   8369    8369 
   Size:       8852
 
SfcI C'TryA_G

Cuts at:     33     709     977    1147    1599    1705    1887    1984    2539 
   Size:        676     268     170     452     106     182      97     555

Cuts at:   2539    2893    4203    5895    6580    7573    7764      33 
   Size:        354    1310    1692     685     993     191    1121

  Fragments arranged by size: 

                1692    1310    1121     993     685     676     555     452
                 354     268     191     182     170     106      97
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2664    2664 
   Size:       8852
 
SfoI GGC'GCC

Cuts at:   6284    6532    6284 
   Size:        248    8604
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8852
 
SmaI CCC'GGG

Cuts at:   5874    5874 
   Size:       8852
 
SmlI C'TyrA_G

Cuts at:    171     691    2494    3307    3520    4864    5494    6007    6922 
   Size:        520    1803     813     213    1344     630     513     915

Cuts at:   6922    7414    7676     171 
   Size:        492     262    1347

  Fragments arranged by size: 

                1803    1347    1344     915     813     630     520     513
                 492     262     213
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       8852
 
SphI G_CATG'C

Cuts at:    753    2473    5905    6405     753 
   Size:       1720    3432     500    3200

  Fragments arranged by size: 

                3432    3200    1720     500
 
StuI AGG'CCT

Cuts at:    832    1187    2197    2658     832 
   Size:        355    1010     461    7026

  Fragments arranged by size: 

                7026    1010     461     355
 
StyI C'CwwG_G

Cuts at:    107     768     858    1057    1319    1584    1839    1871    3122 
   Size:        661      90     199     262     265     255      32    1251

Cuts at:   3122    3254    3695    5114    5183    5257    5303    5986    6340 
   Size:        132     441    1419      69      74      46     683     354

Cuts at:   6340     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1419    1251     683     661     441     354     265
                 262     255     199     132      90      74      69      46
                  32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1989    2239    2278    3052    6435    6568    7027    7236    1989 
   Size:        250      39     774    3383     133     459     209    3605

  Fragments arranged by size: 

                3605    3383     774     459     250     209     133      39
            
 
TatI w'GTAC_w

Cuts at:    797    2824    4194    4799    4829    5321    5741    8367     797 
   Size:       2027    1370     605      30     492     420    2626    1282

  Fragments arranged by size: 

                2626    2027    1370    1282     605     492     420      30
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1964    1996    3854    3886    4774    4806    8369    8401    1964 
   Size:         32    1858      32     888      32    3563      32    2415

  Fragments arranged by size: 

                3563    2415    1858     888      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2919    4155    6649    2919 
   Size:       1236    2494    5122

  Fragments arranged by size: 

                5122    2494    1236
 
XbaI T'CTAG_A

Cuts at:   5883    5883 
   Size:       8852
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    861    4710    6347     861 
   Size:       3849    1637    3366

  Fragments arranged by size: 

                3849    3366    1637
 
XmaI C'CCGG_G

Cuts at:   5872    5872 
   Size:       8852
 
XmnI GAAnn'nnTTC

Cuts at:   4588    4588 
   Size:       8852
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AscI    AvrII     BaeI    BfrBI     ClaI
    EcoRV    FspAI     HpaI     MfeI     NotI     NsiI     PacI     PsiI
    PspXI     PsrI    SacII    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com