Restriction map for pF1KB1123
##################################
# NO CUT ENZYMES (   43 enzymes) #
##################################

AclI        AflII       AgeI        Alw44I      ApaLI       
AscI        AvrII       BbrPI       BfrI        BlnI        
BsiWI       BssHII      Bst1107I    BstZ17I     CpoI        
DraI        FseI        HindIII     KspI        MfeI        
MluI        MunI        NaeI        NgoMIV      NotI        
NruI        PacI        PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SgrAI       SnaBI       
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        5861  GACGTC               GACGT^C
Acc65I       5897  GGTACC               G^GTACC
AccIII       6816  TCCGGA               T^CCGGA
AhdI         3523  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      7429  GAATCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5897  GGTACC               G^GTACC
AspEI        3523  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2189  TGCGCA               TGC^GCA
BbeI         3310  GGCGCC               GGCGC^C
BsaAI         430  TACGTG               [CT]AC^GT[GA]
BseAI        6816  TCCGGA               T^CCGGA
BspDI        5131  ATCGAT               AT^CGAT
BspEI        6816  TCCGGA               T^CCGGA
BsrBI        1379  GAGCGG               CCGCTC(-3/-3)
BsrFI        1076  GCCGGT               [GA]^CCGG[CT]
BstEII       5085  GGTGACC              G^GT[ACGT]ACC
BstPI        5085  GGTGACC              G^GT[ACGT]ACC
Cfr10I       1076  GCCGGT               [GA]^CCGG[CT]
ClaI         5131  ATCGAT               AT^CGAT
Eam1105I     3523  GACACGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      5085  GGTGACC              G^GT[ACGT]ACC
EcoRV        1498  GATATC               GAT^ATC
FspI         2189  TGCGCA               TGC^GCA
HpaI         6613  GTTAAC               GTT^AAC
KasI         3310  GGCGCC               G^GCGCC
KpnI         5897  GGTACC               GGTAC^C
MroI         6816  TCCGGA               T^CCGGA
NarI         3310  GGCGCC               GG^CGCC
NheI          271  GCTAGC               G^CTAGC
PshAI        5887  GACATTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI         3310  GGCGCC               GGC^GCC
SmaI         2726  CCCGGG               CCC^GGG
SpeI         2791  ACTAGT               A^CTAGT
XbaI         4236  TCTAGA               T^CTAGA
XmaCI        2726  CCCGGG               C^CCGGG
XmaI         2726  CCCGGG               C^CCGGG
XmnI         7429  GAATCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccI          177  GTCTAC               GT^[AC][GT]AC
             5943  GTCTAC              
AccII        1360  CGCG                 CG^CG
             5431  CGCG                
AflIII       5108  ACATGT               A^C[GA][CT]GT
             5990  ACATGT              
AocI         1720  CCTGAGG              CC^T[ACGT]AGG
             5080  CCTCAGG             
AseI         2334  ATTAAT               AT^TAAT
             4255  ATTAAT              
AsnI         2334  ATTAAT               AT^TAAT
             4255  ATTAAT              
AspI         3426  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7234  GACAGTGTC           
BcgI          674  CGAAGGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1970  GCAAACTGCTCG        
BciVI        3655  GGATAC               GTATCC(6/5)
             5703  GGATAC              
BclI         1110  TGATCA               T^GATCA
             2381  TGATCA              
BlpI         1965  GCTTAGC              GC^T[ACGT]AGC
             3858  GCTGAGC             
Bpu1102I     1965  GCTTAGC              GC^T[ACGT]AGC
             3858  GCTGAGC             
BsaBI        2196  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2865  GATGCGGATC          
BsaWI          10  ACCGGA               [AT]^CCGG[AT]
             6816  TCCGGA              
Bsp1407I      963  TGTACA               T^GTACA
             5636  TGTACA              
BspLU11I     5108  ACATGT               A^CATGT
             5990  ACATGT              
BsrGI         963  TGTACA               T^GTACA
             5636  TGTACA              
BssSI        4360  CACGAG               CACGAG(-5/-1)
             5684  CACGAG              
BstBI        3479  TTCGAA               TT^CGAA
             5392  TTCGAA              
BstUI        1360  CGCG                 CG^CG
             5431  CGCG                
Bsu36I       1720  CCTGAGG              CC^T[ACGT]AGG
             5080  CCTCAGG             
CelII        1965  GCTTAGC              GC^T[ACGT]AGC
             3858  GCTGAGC             
DraIII       2695  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             7201  CACACAGTG           
DrdI          355  GACCCAGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1435  GACGCCACAGTC        
EagI         4286  CGGCCG               C^GGCCG
             5501  CGGCCG              
EclXI        4286  CGGCCG               C^GGCCG
             5501  CGGCCG              
Eco52I       4286  CGGCCG               C^GGCCG
             5501  CGGCCG              
Eco81I       1720  CCTGAGG              CC^T[ACGT]AGG
             5080  CCTCAGG             
EcoRI        3118  GAATTC               G^AATTC
             4084  GAATTC              
EcoT22I      3247  ATGCAT               ATGCA^T
             3728  ATGCAT              
FbaI         1110  TGATCA               T^GATCA
             2381  TGATCA              
MamI         2196  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2865  GATGCGGATC          
MvnI         1360  CGCG                 CG^CG
             5431  CGCG                
NsiI         3247  ATGCAT               ATGCA^T
             3728  ATGCAT              
NspV         3479  TTCGAA               TT^CGAA
             5392  TTCGAA              
PaeR7I       1469  CTCGAG               C^TCGAG
             4450  CTCGAG              
PflFI        3426  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7234  GACAGTGTC           
PshBI        2334  ATTAAT               AT^TAAT
             4255  ATTAAT              
SapI         3069  GAAGAGC              GCTCTTC(1/4)
             6600  GCTCTTC             
ScaI          893  AGTACT               AGT^ACT
             5321  AGTACT              
SfuI         3479  TTCGAA               TT^CGAA
             5392  TTCGAA              
SspBI         963  TGTACA               T^GTACA
             5636  TGTACA              
Tth111I      3426  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             7234  GACAGTGTC           
XhoI         1469  CTCGAG               C^TCGAG
             4450  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco47III    EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SphI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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