Restriction map for pF1KB1123
 (Circular) (Six-base) MAPSORT of: pF1KB1123.seq  Check: 1197  from: 1  to: 10760



pF1KB1123  10760 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1527    1990    2610    1527 
   Size:        463     620    9677

  Fragments arranged by size: 

                9677     620     463
 
AatII G_ACGT'C

Cuts at:   5973    5973 
   Size:      10760
 
AccI GT'mk_AC

Cuts at:    286    6052    7798     286 
   Size:       5766    1746    3248

  Fragments arranged by size: 

                5766    3248    1746
 
Acc65I G'GTAC_C

Cuts at:   6005    7776    6005 
   Size:       1771    8989
 
AclI AA'CG_TT

Cuts at:  10600   10600 
   Size:      10760
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2455    2743    3503    4003    4181    6112    6322    8585    9017 
   Size:        288     760     500     178    1931     210    2263     432

Cuts at:   9017    9764   10032    2455 
   Size:        747     268    3183

  Fragments arranged by size: 

                3183    2263    1931     760     747     500     432     288
                 268     210     178
 
AfeI AGC'GCT

Cuts at:   2152    3512    3730    2152 
   Size:       1360     218    9182

  Fragments arranged by size: 

                9182    1360     218
 
AflIII A'CryG_T

Cuts at:   5216    6098    8112    9216   10193    5216 
   Size:        882    2014    1104     977    5783

  Fragments arranged by size: 

                5783    2014    1104     977     882
 
AgeI A'CCGG_T

Cuts at:   9148    9148 
   Size:      10760
 
AhdI GACnn_n'nnGTC

Cuts at:   3636    3636 
   Size:      10760
 
AleI CACnn'nnGTG

Cuts at:   1174    1663    4791    1174 
   Size:        489    3128    7143

  Fragments arranged by size: 

                7143    3128     489
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    197    1106    1585    4267    4809    5100    6928     197 
   Size:        909     479    2682     542     291    1828    4029

  Fragments arranged by size: 

                4029    2682    1828     909     542     479     291
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3127    3159    4301    4333    3127 
   Size:         32    1142      32    9554

  Fragments arranged by size: 

                9554    1142      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    987    2107    2470    3075    3153    5334    6300    8065    9632 
   Size:       1120     363     605      78    2181     966    1765    1567

Cuts at:   9632     987 
   Size:       2115

  Fragments arranged by size: 

                2181    2115    1765    1567    1120     966     605     363
                  78
 
ApaI G_GGCC'C

Cuts at:   4023    4219    6292    4023 
   Size:        196    2073    8491

  Fragments arranged by size: 

                8491    2073     196
 
ApaLI G'TGCA_C

Cuts at:   9530    9530 
   Size:      10760
 
ApoI r'AATT_y

Cuts at:    544    1213    1885    2715    3136    3226    3304    3674    4192 
   Size:        669     672     830     421      90      78     370     518

Cuts at:   4192    7475    7764   10113   10369     544 
   Size:       3283     289    2349     256     935

  Fragments arranged by size: 

                3283    2349     935     830     672     669     518     421
                 370     289     256      90      78
 
AseI AT'TA_AT

Cuts at:     20    2443    4364      20 
   Size:       2423    1921    6416

  Fragments arranged by size: 

                6416    2423    1921
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10760
 
AvaI C'yCGr_G

Cuts at:   1577    2834    4558    4718    4933    6807    7208    7780    1577 
   Size:       1257    1724     160     215    1874     401     572    4557

  Fragments arranged by size: 

                4557    1874    1724    1257     572     401     215     160
            
 
BamHI G'GATC_C

Cuts at:    887    2790    3789    7785     887 
   Size:       1903     999    3996    3862

  Fragments arranged by size: 

                3996    3862    1903     999
 
BanI G'GyrC_C

Cuts at:   2381    3418    5751    6005    7742    7776    8190    8438    8473 
   Size:       1037    2333     254    1737      34     414     248      35

Cuts at:   8473    2381 
   Size:       4668

  Fragments arranged by size: 

                4668    2333    1737    1037     414     254     248      35
                  34
 
BanII G_rGCy'C

Cuts at:    239     564    1628    2678    3607    4023    4118    4219    6266 
   Size:        325    1064    1050     929     416      95     101    2047

Cuts at:   6266    6292    7774    8804     239 
   Size:         26    1482    1030    2195

  Fragments arranged by size: 

                2195    2047    1482    1064    1050    1030     929     416
                 325     101      95      26
 
BbeI G_GCGC'C

Cuts at:   3422    8194    8442    3422 
   Size:       4772     248    5740

  Fragments arranged by size: 

                5740    4772     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1545    2731    3342    3632    5057    1545 
   Size:       1186     611     290    1425    7248

  Fragments arranged by size: 

                7248    1425    1186     611     290
 
BbvCI CC'TCA_GC

Cuts at:   5927    6455    6766    5927 
   Size:        528     311    9921

  Fragments arranged by size: 

                9921     528     311
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     771     805    2067    2101      90 
   Size:         34     647      34    1262      34    8749

  Fragments arranged by size: 

                8749    1262     647      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3757    5805    8658    9419   10760    3757 
   Size:       2048    2853     761    1341    3757

  Fragments arranged by size: 

                3757    2853    2048    1341     761
 
BclI T'GATC_A

Cuts at:   1218    2489    8280    1218 
   Size:       1271    5791    3698

  Fragments arranged by size: 

                5791    3698    1271
 
BfrBI ATG'CAT

Cuts at:   3357    3838    3357 
   Size:        481   10279
 
BglI GCCn_nnn'nGGC

Cuts at:   1772    4489    4642    4651    5087    5572    5608    6818    7286 
   Size:       2717     153       9     436     485      36    1210     468

Cuts at:   7286    1772 
   Size:       5246

  Fragments arranged by size: 

                5246    2717    1210     485     468     436     153      36
                   9
 
BglII A'GATC_T

Cuts at:   1011    4958    6678    8275   10312    1011 
   Size:       3947    1720    1597    2037    1459

  Fragments arranged by size: 

                3947    2037    1720    1597    1459
 
BlpI GC'TnA_GC

Cuts at:   2074    3967    7872    2074 
   Size:       1893    3905    4962

  Fragments arranged by size: 

                4962    3905    1893
 
Bme1580I G_kGCm'C

Cuts at:   2370    2384    4023    4219    5232    6292    7747    8385    8478 
   Size:         14    1639     196    1013    1060    1455     638      93

Cuts at:   8478    9534    2370 
   Size:       1056    3596

  Fragments arranged by size: 

                3596    1639    1455    1060    1056    1013     638     196
                  93      14
 
BmgBI CAC'GTC

Cuts at:   3885    3885 
   Size:      10760
 
BmrI ACTGGGnnnn_n'

Cuts at:    460    1148    1332    7972    8053    8388   10532     460 
   Size:        688     184    6640      81     335    2144     688

  Fragments arranged by size: 

                6640    2144     688     688     335     184      81
 
BmtI G_CTAG'C

Cuts at:    383     383 
   Size:      10760
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7645    9127    7645 
   Size:       1482    9278
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    223    1642    2575    3994    4333    4456    4924    5791    6280 
   Size:       1419     933    1419     339     123     468     867     489

Cuts at:   6280    7413     223 
   Size:       1133    3570

  Fragments arranged by size: 

                3570    1419    1419    1133     933     867     489     468
                 339     123
 
Bpu10I CC'TnA_GC

Cuts at:   1369    1678    3817    5927    6455    6766    1369 
   Size:        309    2139    2110     528     311    5363

  Fragments arranged by size: 

                5363    2139    2110     528     311     309
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2722    5053    7936    8815    9307    9605    2722 
   Size:       2331    2883     879     492     298    3877

  Fragments arranged by size: 

                3877    2883    2331     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    841    2394    2981    3936    4621   10464     841 
   Size:       1553     587     955     685    5843    1137

  Fragments arranged by size: 

                5843    1553    1137     955     685     587
 
BsaAI yAC'GTr

Cuts at:    540    8113     540 
   Size:       7573    3187
 
BsaBI GATnn'nnATC

Cuts at:      6    2308    2977    8274   10311       6 
   Size:       2302     669    5297    2037     455

  Fragments arranged by size: 

                5297    2302    2037     669     455
 
BsaHI Gr'CG_yC

Cuts at:   1544    2137    3419    5970    8191    8439    9141   10637    1544 
   Size:        593    1282    2551    2221     248     702    1496    1667

  Fragments arranged by size: 

                2551    2221    1667    1496    1282     702     593     248
            
 
BsaWI w'CCGG_w

Cuts at:    118    6924    7949    8470    9148    9422    9569    9980     118 
   Size:       6806    1025     521     678     274     147     411     898

  Fragments arranged by size: 

                6806    1025     898     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1171    1201    2374    2404    3547    3577    4762    4792    1171 
   Size:         30    1173      30    1143      30    1185      30    7139

  Fragments arranged by size: 

                7139    1185    1173    1143      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    295     439    1222    3799    4921    6163     295 
   Size:        144     783    2577    1122    1242    4892

  Fragments arranged by size: 

                4892    2577    1242    1122     783     144
 
BseYI C'CCAG_C

Cuts at:    573     770    1079    1107    2376    2949    3153    3323    3657 
   Size:        197     309      28    1269     573     204     170     334

Cuts at:   3657    4016    4254    4539    5291    6821    6991    7030    8185 
   Size:        359     238     285     752    1530     170      39    1155

Cuts at:   8185    8943    9520   10016     573 
   Size:        758     577     496    1317

  Fragments arranged by size: 

                1530    1317    1269    1155     758     752     577     573
                 496     359     334     309     285     238     204     197
                 170     170      39      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2229    3088    3865    4450    5320    6381    6829    7153    2229 
   Size:        859     777     585     870    1061     448     324    5836

  Fragments arranged by size: 

                5836    1061     870     859     777     585     448     324
            
 
BsiEI CG_ry'CG

Cuts at:    104    4397    5457    5612    8302    8348    9556     104 
   Size:       4293    1060     155    2690      46    1208    1308

  Fragments arranged by size: 

                4293    2690    1308    1208    1060     155      46
 
BsiHKAI G_wGCw'C

Cuts at:    239    1670    1952    2336    2678    3259    3743    4118    5410 
   Size:       1431     282     384     342     581     484     375    1292

Cuts at:   5410    5453    5674    6110    7337    7774    8552    8742    9534 
   Size:         43     221     436    1227     437     778     190     792

Cuts at:   9534     239 
   Size:       1465

  Fragments arranged by size: 

                1465    1431    1292    1227     792     778     581     484
                 437     436     384     375     342     282     221     190
                  43
 
BsmI GAATG_Cn'

Cuts at:    411    1309    6563     411 
   Size:        898    5254    4608

  Fragments arranged by size: 

                5254    4608     898
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3954    5156    5977    7553    3954 
   Size:       1202     821    1576    7161

  Fragments arranged by size: 

                7161    1576    1202     821
 
Bsp1286I G_dGCh'C

Cuts at:    239     564    1628    1670    1952    2336    2370    2384    2678 
   Size:        325    1064      42     282     384      34      14     294

Cuts at:   2678    3259    3607    3743    4023    4118    4219    5232    5410 
   Size:        581     348     136     280      95     101    1013     178

Cuts at:   5410    5453    5674    6110    6266    6292    7337    7747    7774 
   Size:         43     221     436     156      26    1045     410      27

Cuts at:   7774    8385    8478    8552    8742    8804    9534     239 
   Size:        611      93      74     190      62     730    1465

  Fragments arranged by size: 

                1465    1064    1045    1013     730     611     581     436
                 410     384     348     325     294     282     280     221
                 190     178     156     136     101      95      93      74
                  62      43      42      34      27      26      14
 
BspEI T'CCGG_A

Cuts at:   6924    9980    6924 
   Size:       3056    7704
 
BspHI T'CATG_A

Cuts at:   3218    3236    5046    7650    9936    3218 
   Size:         18    1810    2604    2286    4042

  Fragments arranged by size: 

                4042    2604    2286    1810      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    132    1527    1990    2610    3326    3446    4823    7810    8024 
   Size:       1395     463     620     716     120    1377    2987     214

Cuts at:   8024    8326    8707     132 
   Size:        302     381    2185

  Fragments arranged by size: 

                2987    2185    1395    1377     716     620     463     381
                 302     214     120
 
BsrBI CCG'CTC

Cuts at:     50    1489    9052    9106   10590   10755      50 
   Size:       1439    7563      54    1484     165      55

  Fragments arranged by size: 

                7563    1484    1439     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    165     594    1538    1589    4844    5933    8672     165 
   Size:        429     944      51    3255    1089    2739    2253

  Fragments arranged by size: 

                3255    2739    2253    1089     944     429      51
 
BsrFI r'CCGG_y

Cuts at:   1184    8758    8939    9148    1184 
   Size:       7574     181     209    2796

  Fragments arranged by size: 

                7574    2796     209     181
 
BsrGI T'GTAC_A

Cuts at:   1071    5744    1071 
   Size:       4673    6087
 
BssSI C'ACGA_G

Cuts at:   4468    5792    9031    9389    4468 
   Size:       1324    3239     358    5839

  Fragments arranged by size: 

                5839    3239    1324     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3075    3447    3813    4553    5334    8137    3075 
   Size:        372     366     740     781    2803    5698

  Fragments arranged by size: 

                5698    2803     781     740     372     366
 
BstBI TT'CG_AA

Cuts at:   3588    5501    9121    3588 
   Size:       1913    3620    5227

  Fragments arranged by size: 

                5227    3620    1913
 
BstEII G'GTnAC_C

Cuts at:   5193    5193 
   Size:      10760
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1176    1788    1842    3415    3627    5961    7266    1176 
   Size:        612      54    1573     212    2334    1305    4670

  Fragments arranged by size: 

                4670    2334    1573    1305     612     212      54
 
BstYI r'GATC_y

Cuts at:      1     887    1011    1958    2103    2225    2460    2790    2978 
   Size:        886     124     947     145     122     235     330     188

Cuts at:   2978    3789    4053    4439    4958    5721    6678    6881    7163 
   Size:        811     264     386     519     763     957     203     282

Cuts at:   7163    7785    8252    8275    8610    8856    9868    9954    9966 
   Size:        622     467      23     335     246    1012      86      12

Cuts at:   9966   10312       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     957     947     886     811     763     622     519
                 467     449     386     346     335     330     282     264
                 246     235     203     188     145     124     122      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1829    5189    1829 
   Size:       3360    7400
 
BtgI C'CryG_G

Cuts at:    254     509     891    2960    3014    3626    4874    5606     254 
   Size:        255     382    2069      54     612    1248     732    5408

  Fragments arranged by size: 

                5408    2069    1248     732     612     382     255      54
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1853    3980    4140    5286    5555    5678    6886    8710    8891 
   Size:       2127     160    1146     269     123    1208    1824     181

Cuts at:   8891    9054    1853 
   Size:        163    3559

  Fragments arranged by size: 

                3559    2127    1824    1208    1146     269     181     163
                 160     123
 
BtsI GCAGTG_nn'

Cuts at:   1643    1658    1670    2057    3490    4600    4759    5495    5594 
   Size:         15      12     387    1433    1110     159     736      99

Cuts at:   5594    5615    5627    6841    8003    1643 
   Size:         21      12    1214    1162    4400

  Fragments arranged by size: 

                4400    1433    1214    1162    1110     736     387     159
                  99      21      15      12      12
 
ClaI AT'CG_AT

Cuts at:   5240    5240 
   Size:      10760
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    399     434    1520    1555     399 
   Size:         35    1086      35    9604

  Fragments arranged by size: 

                9604    1086      35      35
 
DraI TTT'AAA

Cuts at:   7759    7759 
   Size:      10760
 
DraIII CAC_nnn'GTG

Cuts at:   2808    7314    2808 
   Size:       4506    6254
 
DrdI GACnn_nn'nnGTC

Cuts at:    469    1549    8466    9324     469 
   Size:       1080    6917     858    1905

  Fragments arranged by size: 

                6917    1905    1080     858
 
EaeI y'GGCC_r

Cuts at:    371     498     632     695     854    2022    2237    2609    2651 
   Size:        127     134      63     159    1168     215     372      42

Cuts at:   2651    3825    4046    4394    5609    5854    6164    6734    7004 
   Size:       1174     221     348    1215     245     310     570     270

Cuts at:   7004    7352    7461    8345    8519    8910    8937   10282     371 
   Size:        348     109     884     174     391      27    1345     849

  Fragments arranged by size: 

                1345    1215    1174    1168     884     849     570     391
                 372     348     348     310     270     245     221     215
                 174     159     134     127     109      63      42      27
            
 
EagI C'GGCC_G

Cuts at:   4394    5609    8345    4394 
   Size:       1215    2736    6809

  Fragments arranged by size: 

                6809    2736    1215
 
EarI CTCTTCn'nnn_

Cuts at:   3172    3776    4063    4780    5218    6197    6715    6758    8783 
   Size:        604     287     717     438     979     518      43    2025

Cuts at:   8783    8993    3172 
   Size:        210    4939

  Fragments arranged by size: 

                4939    2025     979     717     604     518     438     287
                 210      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2191    2401    4942    8968    9278    9424   10570    2191 
   Size:        210    2541    4026     310     146    1146    2381

  Fragments arranged by size: 

                4026    2541    2381    1146     310     210     146
 
EcoICRI GAG'CTC

Cuts at:    237    2676    4116    7772     237 
   Size:       2439    1440    3656    3225

  Fragments arranged by size: 

                3656    3225    2439    1440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    223    1642    2455    2575    2743    3503    3994    4003    4181 
   Size:       1419     813     120     168     760     491       9     178

Cuts at:   4181    4333    4456    4924    5791    6112    6280    6322    7413 
   Size:        152     123     468     867     321     168      42    1091

Cuts at:   7413    8585    9017    9764   10032     223 
   Size:       1172     432     747     268     951

  Fragments arranged by size: 

                1419    1172    1091     951     867     813     760     747
                 491     468     432     321     268     178     168     168
                 152     123     120      42       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1828    2170    3079    3904    4027    6202    1828 
   Size:        342     909     825     123    2175    6386

  Fragments arranged by size: 

                6386    2175     909     825     342     123
 
EcoO109I rG'GnC_Cy

Cuts at:    705    1145    1785    3865    4020    4215    4216    4327    4770 
   Size:        440     640    2080     155     195       1     111     443

Cuts at:   4770    4812    6018    6288    6289    6393    6411    6510    6611 
   Size:         42    1206     270       1     104      18      99     101

Cuts at:   6611    7581    7899     705 
   Size:        970     318    3566

  Fragments arranged by size: 

                3566    2080    1206     970     640     443     440     318
                 270     195     155     111     104     101      99      42
                  18       1       1
 
EcoRI G'AATT_C

Cuts at:   3226    4192    7764    3226 
   Size:        966    3572    6222

  Fragments arranged by size: 

                6222    3572     966
 
EcoRV GAT'ATC

Cuts at:   1608    1608 
   Size:      10760
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   8779    8779 
   Size:      10760
 
FspI TGC'GCA

Cuts at:   2299    8541    2299 
   Size:       6242    4518
 
HaeII r_GCGC'y

Cuts at:   2154    3422    3514    3732    3920    5441    8194    8442    9464 
   Size:       1268      92     218     188    1521    2753     248    1022

Cuts at:   9464   10443    2154 
   Size:        979    2471

  Fragments arranged by size: 

                2753    2471    1521    1268    1022     979     248     218
                 188      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2088    2120    2744    2776    3547    3579    3622    3654    3701 
   Size:         32     624      32     771      32      43      32      47

Cuts at:   3701    3733    3979    4011    4762    4794    4842    4874    4931 
   Size:         32     246      32     751      32      48      32      57

Cuts at:   4931    4934    4963    4966    5710    5742    6152    6184    6733 
   Size:          3      29       3     744      32     410      32     549

Cuts at:   6733    6765    6770    6802    7438    7470    8602    8632    8634 
   Size:         32       5      32     636      32    1132      30       2

Cuts at:   8634    8664    2088 
   Size:         30    4184

  Fragments arranged by size: 

                4184    1132     771     751     744     636     624     549
                 410     246      57      48      47      43      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      30      30      29       5       3
                   3       2
 
HincII GTy'rAC

Cuts at:   2916    4582    6723    7256    7799    2916 
   Size:       1666    2141     533     543    5877

  Fragments arranged by size: 

                5877    2141    1666     543     533
 
HpaI GTT'AAC

Cuts at:   6723    6723 
   Size:      10760
 
KasI G'GCGC_C

Cuts at:   3418    8190    8438    3418 
   Size:       4772     248    5740

  Fragments arranged by size: 

                5740    4772     248
 
KpnI G_GTAC'C

Cuts at:   6009    7780    6009 
   Size:       1771    8989
 
MluI A'CGCG_T

Cuts at:  10193   10193 
   Size:      10760
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1338    2309    6456    7157    7696    9431    9615    1338 
   Size:        971    4147     701     539    1735     184    2483

  Fragments arranged by size: 

                4147    2483    1735     971     701     539     184
 
MscI TGG'CCA

Cuts at:    373     634     856    2024    2239    2611    2653    3827    4048 
   Size:        261     222    1168     215     372      42    1174     221

Cuts at:   4048    5856    6166    6736    7006    7463    8521     373 
   Size:       1808     310     570     270     457    1058    2612

  Fragments arranged by size: 

                2612    1808    1174    1168    1058     570     457     372
                 310     270     261     222     221     215      42
 
MslI CAynn'nnrTG

Cuts at:    391     478     691    1174    1567    1663    2030    2584    2764 
   Size:         87     213     483     393      96     367     554     180

Cuts at:   2764    2818    3217    3625    3685    4708    4791    6139    7090 
   Size:         54     399     408      60    1023      83    1348     951

Cuts at:   7090    7315    7354    8749    8876     391 
   Size:        225      39    1395     127    2275

  Fragments arranged by size: 

                2275    1395    1348    1023     951     554     483     408
                 399     393     367     225     213     180     127      96
                  87      83      60      54      39
 
MspA1I CmG'CkG

Cuts at:    142     532     610     987    1402    1800    2953    3153    3327 
   Size:        390      78     377     415     398    1153     200     174

Cuts at:   3327    3411    3657    4393    4663    5382    5462    5570    5700 
   Size:         84     246     736     270     719      80     108     130

Cuts at:   5700    6450    6991    7871    8185    8545    9558    9803     142 
   Size:        750     541     880     314     360    1013     245    1099

  Fragments arranged by size: 

                1153    1099    1013     880     750     736     719     541
                 415     398     390     377     360     314     270     246
                 245     200     174     130     108      84      80      78
            
 
NaeI GCC'GGC

Cuts at:   8941    8941 
   Size:      10760
 
NarI GG'CG_CC

Cuts at:   3419    8191    8439    3419 
   Size:       4772     248    5740

  Fragments arranged by size: 

                5740    4772     248
 
NcoI C'CATG_G

Cuts at:    254     509     891    2960    3014    3626    4874     254 
   Size:        255     382    2069      54     612    1248    6140

  Fragments arranged by size: 

                6140    2069    1248     612     382     255      54
 
NdeI CA'TA_TG

Cuts at:   4284    4866    6537    4284 
   Size:        582    1671    8507

  Fragments arranged by size: 

                8507    1671     582
 
NgoMIV G'CCGG_C

Cuts at:   8939    8939 
   Size:      10760
 
NheI G'CTAG_C

Cuts at:    379     379 
   Size:      10760
 
NsiI A_TGCA'T

Cuts at:   3359    3840    3359 
   Size:        481   10279
 
NspI r_CATG'y

Cuts at:    607    1572    1779    2376    2589    3342    4551    4893    5220 
   Size:        965     207     597     213     753    1209     342     327

Cuts at:   5220    5259    5299    5482    6102    6249    6274    6976    7307 
   Size:         39      40     183     620     147      25     702     331

Cuts at:   7307    7813    8313    9220     607 
   Size:        506     500     907    2147

  Fragments arranged by size: 

                2147    1209     965     907     753     702     620     597
                 506     500     342     331     327     213     207     183
                 147      40      39      25
 
PasI CC'CwG_GG

Cuts at:   1416    2637    3051    1416 
   Size:       1221     414    9125

  Fragments arranged by size: 

                9125    1221     414
 
PciI A'CATG_T

Cuts at:   5216    6098    9216    5216 
   Size:        882    3118    6760

  Fragments arranged by size: 

                6760    3118     882
 
PflMI CCAn_nnn'nTGG

Cuts at:   1416    1800    3153    6173    1416 
   Size:        384    1353    3020    6003

  Fragments arranged by size: 

                6003    3020    1353     384
 
PfoI T'CCnGG_A

Cuts at:   2228    2591    3131    3565    5807    6857    2228 
   Size:        363     540     434    2242    1050    6131

  Fragments arranged by size: 

                6131    2242    1050     540     434     363
 
PmeI GTTT'AAAC

Cuts at:   7759    7759 
   Size:      10760
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1020    1052    9899    9931    1020 
   Size:         32    8847      32    1849

  Fragments arranged by size: 

                8847    1849      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    705    1145    3865    4770    4812    6393    6411    6510     705 
   Size:        440    2720     905      42    1581      18      99    4955

  Fragments arranged by size: 

                4955    2720    1581     905     440      99      42      18
            
 
PshAI GACnn'nnGTC

Cuts at:   5999    5999 
   Size:      10760
 
PspOMI G'GGCC_C

Cuts at:   4019    4215    6288    4019 
   Size:        196    2073    8491

  Fragments arranged by size: 

                8491    2073     196
 
PspXI vC'TCGA_Gb

Cuts at:   1577    4558    1577 
   Size:       2981    7779
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   7050    7082    7050 
   Size:         32   10728
 
PstI C_TGCA'G

Cuts at:    176    1665    3751    4662    4979    5429    5705    7807    8492 
   Size:       1489    2086     911     317     450     276    2102     685

Cuts at:   8492     176 
   Size:       2444

  Fragments arranged by size: 

                2444    2102    2086    1489     911     685     450     317
                 276
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      10760
 
PvuII CAG'CTG

Cuts at:    610     987    1800    2953    3153    3327    3411    3657    4663 
   Size:        377     813    1153     200     174      84     246    1006

Cuts at:   4663    5382    5462    6450    6991    8185    8545     610 
   Size:        719      80     988     541    1194     360    2825

  Fragments arranged by size: 

                2825    1194    1153    1006     988     813     719     541
                 377     360     246     200     174      84      80
 
RsrII CG'GwC_CG

Cuts at:   8955    8955 
   Size:      10760
 
SacI G_AGCT'C

Cuts at:    239    2678    4118    7774     239 
   Size:       2439    1440    3656    3225

  Fragments arranged by size: 

                3656    3225    2439    1440
 
SalI G'TCGA_C

Cuts at:   7797    7797 
   Size:      10760
 
SanDI GG'GwC_CC

Cuts at:    705     705 
   Size:      10760
 
SapI GCTCTTCn'nnn_

Cuts at:   3172    6715    8783    8993    3172 
   Size:       3543    2068     210    4939

  Fragments arranged by size: 

                4939    3543    2068     210
 
SbfI CC_TGCA'GG

Cuts at:   7807    7807 
   Size:      10760
 
ScaI AGT'ACT

Cuts at:   1003    5431   10277    1003 
   Size:       4428    4846    1486

  Fragments arranged by size: 

                4846    4428    1486
 
SexAI A'CCwGG_T

Cuts at:   2213    2384    4450    2213 
   Size:        171    2066    8523

  Fragments arranged by size: 

                8523    2066     171
 
SfcI C'TryA_G

Cuts at:     33     172     734    1661    2679    2892    3747    4375    4658 
   Size:        139     562     927    1018     213     855     628     283

Cuts at:   4658    4975    5389    5425    5433    5701    5914    6864    7293 
   Size:        317     414      36       8     268     213     950     429

Cuts at:   7293    7803    8488    9481    9672      33 
   Size:        510     685     993     191    1121

  Fragments arranged by size: 

                1121    1018     993     950     927     855     685     628
                 562     510     429     414     317     283     268     213
                 213     191     139      36       8
 
SfoI GGC'GCC

Cuts at:   3420    8192    8440    3420 
   Size:       4772     248    5740

  Fragments arranged by size: 

                5740    4772     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10760
 
SmaI CCC'GGG

Cuts at:   2836    7782    2836 
   Size:       4946    5814
 
SmlI C'TyrA_G

Cuts at:   1577    2701    4558    5068    7915    8830    9322    9584    1577 
   Size:       1124    1857     510    2847     915     492     262    2753

  Fragments arranged by size: 

                2847    2753    1857    1124     915     510     492     262
            
 
SpeI A'CTAG_T

Cuts at:     67    2899      67 
   Size:       2832    7928
 
SphI G_CATG'C

Cuts at:   1572    1779    2376    4551    5299    5482    6274    6976    7813 
   Size:        207     597    2175     748     183     792     702     837

Cuts at:   7813    8313    1572 
   Size:        500    4019

  Fragments arranged by size: 

                4019    2175     837     792     748     702     597     500
                 207     183
 
StuI AGG'CCT

Cuts at:   1130    2191    3910    4033    4564    5473    5872    6197    6325 
   Size:       1061    1719     123     531     909     399     325     128

Cuts at:   6325    7112    7280    1130 
   Size:        787     168    4610

  Fragments arranged by size: 

                4610    1719    1061     909     787     531     399     325
                 168     128     123
 
StyI C'CwwG_G

Cuts at:    230     254     488     509     851     891    1094    1125    1781 
   Size:         24     234      21     342      40     203      31     656

Cuts at:   1781    2960    3014    3626    3828    4565    4781    4874    5960 
   Size:       1179      54     612     202     737     216      93    1086

Cuts at:   5960    7894    8248     230 
   Size:       1934     354    2742

  Fragments arranged by size: 

                2742    1934    1179    1086     737     656     612     354
                 342     234     216     203     202      93      54      40
                  31      24      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    565    1224    1784    4246    4741    6415    7067    7142    8343 
   Size:        659     560    2462     495    1674     652      75    1201

Cuts at:   8343    8476    8935    9144     565 
   Size:        133     459     209    2181

  Fragments arranged by size: 

                2462    2181    1674    1201     659     652     560     495
                 459     209     133      75
 
TatI w'GTAC_w

Cuts at:   1001    1071    4919    5429    5744    6218    6368   10275    1001 
   Size:         70    3848     510     315     474     150    3907    1486

  Fragments arranged by size: 

                3907    3848    1486     510     474     315     150      70
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1171    1203    1991    2023    2374    2406    2633    2665    3149 
   Size:         32     788      32     351      32     227      32     484

Cuts at:   3149    3181    4879    4911   10277   10309    1171 
   Size:         32    1698      32    5366      32    1622

  Fragments arranged by size: 

                5366    1698    1622     788     484     351     227      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3537    7345    8557    3537 
   Size:       3808    1212    5740

  Fragments arranged by size: 

                5740    3808    1212
 
XbaI T'CTAG_A

Cuts at:   4344    7791    4344 
   Size:       3447    7313
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1635    2017    2247    2619    4815    5961    7140    7732    8255 
   Size:        382     230     372    2196    1146    1179     592     523

Cuts at:   8255    1635 
   Size:       4140

  Fragments arranged by size: 

                4140    2196    1179    1146     592     523     382     372
                 230
 
XhoI C'TCGA_G

Cuts at:   1577    4558    1577 
   Size:       2981    7779
 
XmaI C'CCGG_G

Cuts at:   2834    7780    2834 
   Size:       4946    5814
 
XmnI GAAnn'nnTTC

Cuts at:   7541    7541 
   Size:      10760
 
ZraI GAC'GTC

Cuts at:   5971    5971 
   Size:      10760
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AscI    AvrII     BaeI    BsiWI   BssHII  BstZ17I     FseI
    FspAI  HindIII     MfeI     NotI     NruI     PacI     PmlI     PsiI
    SacII     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
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