Restriction map for pF1KB1123
 (Linear) (Six-base) MAPSORT of: KIBB1123.seq  Check: 9243  from: 1  to: 7647



KIBB1123.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1419    1882    2502    7647 
   Size:       1419     463     620    5145

  Fragments arranged by size: 

                5145    1419     620     463
 
AatII G_ACGT'C

Cuts at:      0    5865    7647 
   Size:       5865    1782
 
AccI GT'mk_AC

Cuts at:      0     178    5944    7647 
   Size:        178    5766    1703

  Fragments arranged by size: 

                5766    1703     178
 
Acc65I G'GTAC_C

Cuts at:      0    5897    7647 
   Size:       5897    1750
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2347    2635    3395    3895    4073    6004    6214    7647 
   Size:       2347     288     760     500     178    1931     210    1433

  Fragments arranged by size: 

                2347    1931    1433     760     500     288     210     178
            
 
AfeI AGC'GCT

Cuts at:      0    2044    3404    3622    7647 
   Size:       2044    1360     218    4025

  Fragments arranged by size: 

                4025    2044    1360     218
 
AflIII A'CryG_T

Cuts at:      0    5108    5990    7647 
   Size:       5108     882    1657

  Fragments arranged by size: 

                5108    1657     882
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3528    7647 
   Size:       3528    4119
 
AleI CACnn'nnGTG

Cuts at:      0    1066    1555    4683    7647 
   Size:       1066     489    3128    2964

  Fragments arranged by size: 

                3128    2964    1066     489
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      89     998    1477    4159    4701    4992    6820    7647 
   Size:         89     909     479    2682     542     291    1828     827

  Fragments arranged by size: 

                2682    1828     909     827     542     479     291      89
            
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3019    3051    4193    4225    7647 
   Size:       3019      32    1142      32    3422

  Fragments arranged by size: 

                3422    3019    1142      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     879    1999    2362    2967    3045    5226    6192    7647 
   Size:        879    1120     363     605      78    2181     966    1455

  Fragments arranged by size: 

                2181    1455    1120     966     879     605     363      78
            
 
ApaI G_GGCC'C

Cuts at:      0    3915    4111    6184    7647 
   Size:       3915     196    2073    1463

  Fragments arranged by size: 

                3915    2073    1463     196
 
ApoI r'AATT_y

Cuts at:      0     436    1105    1777    2607    3028    3118    3196    3566 
   Size:        436     669     672     830     421      90      78     370

Cuts at:   3566    4084    7367    7647 
   Size:        518    3283     280

  Fragments arranged by size: 

                3283     830     672     669     518     436     421     370
                 280      90      78
 
AseI AT'TA_AT

Cuts at:      0    2335    4256    7647 
   Size:       2335    1921    3391

  Fragments arranged by size: 

                3391    2335    1921
 
AvaI C'yCGr_G

Cuts at:      0    1469    2726    4450    4610    4825    6699    7100    7647 
   Size:       1469    1257    1724     160     215    1874     401     547

  Fragments arranged by size: 

                1874    1724    1469    1257     547     401     215     160
            
 
BamHI G'GATC_C

Cuts at:      0     779    2682    3681    7647 
   Size:        779    1903     999    3966

  Fragments arranged by size: 

                3966    1903     999     779
 
BanI G'GyrC_C

Cuts at:      0    2273    3310    5643    5897    7634    7647 
   Size:       2273    1037    2333     254    1737      13

  Fragments arranged by size: 

                2333    2273    1737    1037     254      13
 
BanII G_rGCy'C

Cuts at:      0     131     456    1520    2570    3499    3915    4010    4111 
   Size:        131     325    1064    1050     929     416      95     101

Cuts at:   4111    6158    6184    7647 
   Size:       2047      26    1463

  Fragments arranged by size: 

                2047    1463    1064    1050     929     416     325     131
                 101      95      26
 
BbeI G_GCGC'C

Cuts at:      0    3314    7647 
   Size:       3314    4333
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1437    2623    3234    3524    4949    7647 
   Size:       1437    1186     611     290    1425    2698

  Fragments arranged by size: 

                2698    1437    1425    1186     611     290
 
BbvCI CC'TCA_GC

Cuts at:      0    5819    6347    6658    7647 
   Size:       5819     528     311     989

  Fragments arranged by size: 

                5819     989     528     311
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     663     697    1959    1993    7647 
   Size:        663      34    1262      34    5654

  Fragments arranged by size: 

                5654    1262     663      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3649    5697    7647 
   Size:       3649    2048    1950

  Fragments arranged by size: 

                3649    2048    1950
 
BclI T'GATC_A

Cuts at:      0    1110    2381    7647 
   Size:       1110    1271    5266

  Fragments arranged by size: 

                5266    1271    1110
 
BfrBI ATG'CAT

Cuts at:      0    3249    3730    7647 
   Size:       3249     481    3917

  Fragments arranged by size: 

                3917    3249     481
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1664    4381    4534    4543    4979    5464    5500    6710 
   Size:       1664    2717     153       9     436     485      36    1210

Cuts at:   6710    7178    7647 
   Size:        468     469

  Fragments arranged by size: 

                2717    1664    1210     485     469     468     436     153
                  36       9
 
BglII A'GATC_T

Cuts at:      0     903    4850    6570    7647 
   Size:        903    3947    1720    1077

  Fragments arranged by size: 

                3947    1720    1077     903
 
BlpI GC'TnA_GC

Cuts at:      0    1966    3859    7647 
   Size:       1966    1893    3788

  Fragments arranged by size: 

                3788    1966    1893
 
Bme1580I G_kGCm'C

Cuts at:      0    2262    2276    3915    4111    5124    6184    7639    7647 
   Size:       2262      14    1639     196    1013    1060    1455       8

  Fragments arranged by size: 

                2262    1639    1455    1060    1013     196      14       8
            
 
BmgBI CAC'GTC

Cuts at:      0    3777    7647 
   Size:       3777    3870
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     352    1040    1224    7647 
   Size:        352     688     184    6423

  Fragments arranged by size: 

                6423     688     352     184
 
BmtI G_CTAG'C

Cuts at:      0     275    7647 
   Size:        275    7372
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    7537    7647 
   Size:       7537     110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115    1534    2467    3886    4225    4348    4816    5683 
   Size:        115    1419     933    1419     339     123     468     867

Cuts at:   5683    6172    7305    7647 
   Size:        489    1133     342

  Fragments arranged by size: 

                1419    1419    1133     933     867     489     468     342
                 339     123     115
 
Bpu10I CC'TnA_GC

Cuts at:      0    1261    1570    3709    5819    6347    6658    7647 
   Size:       1261     309    2139    2110     528     311     989

  Fragments arranged by size: 

                2139    2110    1261     989     528     311     309
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2614    4945    7647 
   Size:       2614    2331    2702

  Fragments arranged by size: 

                2702    2614    2331
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733    2286    2873    3828    4513    7647 
   Size:        733    1553     587     955     685    3134

  Fragments arranged by size: 

                3134    1553     955     733     685     587
 
BsaAI yAC'GTr

Cuts at:      0     432    7647 
   Size:        432    7215
 
BsaBI GATnn'nnATC

Cuts at:      0    2200    2869    7647 
   Size:       2200     669    4778

  Fragments arranged by size: 

                4778    2200     669
 
BsaHI Gr'CG_yC

Cuts at:      0    1436    2029    3311    5862    7647 
   Size:       1436     593    1282    2551    1785

  Fragments arranged by size: 

                2551    1785    1436    1282     593
 
BsaWI w'CCGG_w

Cuts at:      0      10    6816    7647 
   Size:         10    6806     831

  Fragments arranged by size: 

                6806     831      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1063    1093    2266    2296    3439    3469    4654    4684 
   Size:       1063      30    1173      30    1143      30    1185      30

Cuts at:   4684    7647 
   Size:       2963

  Fragments arranged by size: 

                2963    1185    1173    1143    1063      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187     331    1114    3691    4813    6055    7647 
   Size:        187     144     783    2577    1122    1242    1592

  Fragments arranged by size: 

                2577    1592    1242    1122     783     187     144
 
BseYI C'CCAG_C

Cuts at:      0     465     662     971     999    2268    2841    3045    3215 
   Size:        465     197     309      28    1269     573     204     170

Cuts at:   3215    3549    3908    4146    4431    5183    6713    6883    6922 
   Size:        334     359     238     285     752    1530     170      39

Cuts at:   6922    7647 
   Size:        725

  Fragments arranged by size: 

                1530    1269     752     725     573     465     359     334
                 309     285     238     204     197     170     170      39
                  28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2121    2980    3757    4342    5212    6273    6721    7045 
   Size:       2121     859     777     585     870    1061     448     324

Cuts at:   7045    7647 
   Size:        602

  Fragments arranged by size: 

                2121    1061     870     859     777     602     585     448
                 324
 
BsiEI CG_ry'CG

Cuts at:      0    4289    5349    5504    7647 
   Size:       4289    1060     155    2143

  Fragments arranged by size: 

                4289    2143    1060     155
 
BsiHKAI G_wGCw'C

Cuts at:      0     131    1562    1844    2228    2570    3151    3635    4010 
   Size:        131    1431     282     384     342     581     484     375

Cuts at:   4010    5302    5345    5566    6002    7229    7647 
   Size:       1292      43     221     436    1227     418

  Fragments arranged by size: 

                1431    1292    1227     581     484     436     418     384
                 375     342     282     221     131      43
 
BsmI GAATG_Cn'

Cuts at:      0     303    1201    6455    7647 
   Size:        303     898    5254    1192

  Fragments arranged by size: 

                5254    1192     898     303
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3846    5048    5869    7445    7647 
   Size:       3846    1202     821    1576     202

  Fragments arranged by size: 

                3846    1576    1202     821     202
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     456    1520    1562    1844    2228    2262    2276 
   Size:        131     325    1064      42     282     384      34      14

Cuts at:   2276    2570    3151    3499    3635    3915    4010    4111    5124 
   Size:        294     581     348     136     280      95     101    1013

Cuts at:   5124    5302    5345    5566    6002    6158    6184    7229    7639 
   Size:        178      43     221     436     156      26    1045     410

Cuts at:   7639    7647 
   Size:          8

  Fragments arranged by size: 

                1064    1045    1013     581     436     410     384     348
                 325     294     282     280     221     178     156     136
                 131     101      95      43      42      34      26      14
                   8
 
BspEI T'CCGG_A

Cuts at:      0    6816    7647 
   Size:       6816     831
 
BspHI T'CATG_A

Cuts at:      0    3110    3128    4938    7542    7647 
   Size:       3110      18    1810    2604     105

  Fragments arranged by size: 

                3110    2604    1810     105      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24    1419    1882    2502    3218    3338    4715    7647 
   Size:         24    1395     463     620     716     120    1377    2932

  Fragments arranged by size: 

                2932    1395    1377     716     620     463     120      24
            
 
BsrBI CCG'CTC

Cuts at:      0    1381    7647 
   Size:       1381    6266
 
BsrDI GCAATG_nn'

Cuts at:      0      57     486    1430    1481    4736    5825    7647 
   Size:         57     429     944      51    3255    1089    1822

  Fragments arranged by size: 

                3255    1822    1089     944     429      57      51
 
BsrFI r'CCGG_y

Cuts at:      0    1076    7647 
   Size:       1076    6571
 
BsrGI T'GTAC_A

Cuts at:      0     963    5636    7647 
   Size:        963    4673    2011

  Fragments arranged by size: 

                4673    2011     963
 
BssSI C'ACGA_G

Cuts at:      0    4360    5684    7647 
   Size:       4360    1324    1963

  Fragments arranged by size: 

                4360    1963    1324
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2967    3339    3705    4445    5226    7647 
   Size:       2967     372     366     740     781    2421

  Fragments arranged by size: 

                2967    2421     781     740     372     366
 
BstBI TT'CG_AA

Cuts at:      0    3480    5393    7647 
   Size:       3480    1913    2254

  Fragments arranged by size: 

                3480    2254    1913
 
BstEII G'GTnAC_C

Cuts at:      0    5085    7647 
   Size:       5085    2562
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1068    1680    1734    3307    3519    5853    7158    7647 
   Size:       1068     612      54    1573     212    2334    1305     489

  Fragments arranged by size: 

                2334    1573    1305    1068     612     489     212      54
            
 
BstYI r'GATC_y

Cuts at:      0     779     903    1850    1995    2117    2352    2682    2870 
   Size:        779     124     947     145     122     235     330     188

Cuts at:   2870    3681    3945    4331    4850    5613    6570    6773    7055 
   Size:        811     264     386     519     763     957     203     282

Cuts at:   7055    7647 
   Size:        592

  Fragments arranged by size: 

                 957     947     811     779     763     592     519     386
                 330     282     264     235     203     188     145     124
                 122
 
Bsu36I CC'TnA_GG

Cuts at:      0    1721    5081    7647 
   Size:       1721    3360    2566

  Fragments arranged by size: 

                3360    2566    1721
 
BtgI C'CryG_G

Cuts at:      0     146     401     783    2852    2906    3518    4766    5498 
   Size:        146     255     382    2069      54     612    1248     732

Cuts at:   5498    7647 
   Size:       2149

  Fragments arranged by size: 

                2149    2069    1248     732     612     382     255     146
                  54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1745    3872    4032    5178    5447    5570    6778    7647 
   Size:       1745    2127     160    1146     269     123    1208     869

  Fragments arranged by size: 

                2127    1745    1208    1146     869     269     160     123
            
 
BtsI GCAGTG_nn'

Cuts at:      0    1535    1550    1562    1949    3382    4492    4651    5387 
   Size:       1535      15      12     387    1433    1110     159     736

Cuts at:   5387    5486    5507    5519    6733    7647 
   Size:         99      21      12    1214     914

  Fragments arranged by size: 

                1535    1433    1214    1110     914     736     387     159
                  99      21      15      12      12
 
ClaI AT'CG_AT

Cuts at:      0    5132    7647 
   Size:       5132    2515
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     291     326    1412    1447    7647 
   Size:        291      35    1086      35    6200

  Fragments arranged by size: 

                6200    1086     291      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2700    7206    7647 
   Size:       2700    4506     441

  Fragments arranged by size: 

                4506    2700     441
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     361    1441    7647 
   Size:        361    1080    6206

  Fragments arranged by size: 

                6206    1080     361
 
EaeI y'GGCC_r

Cuts at:      0     263     390     524     587     746    1914    2129    2501 
   Size:        263     127     134      63     159    1168     215     372

Cuts at:   2501    2543    3717    3938    4286    5501    5746    6056    6626 
   Size:         42    1174     221     348    1215     245     310     570

Cuts at:   6626    6896    7244    7353    7647 
   Size:        270     348     109     294

  Fragments arranged by size: 

                1215    1174    1168     570     372     348     348     310
                 294     270     263     245     221     215     159     134
                 127     109      63      42
 
EagI C'GGCC_G

Cuts at:      0    4286    5501    7647 
   Size:       4286    1215    2146

  Fragments arranged by size: 

                4286    2146    1215
 
EarI CTCTTCn'nnn_

Cuts at:      0    3064    3668    3955    4672    5110    6089    6607    6650 
   Size:       3064     604     287     717     438     979     518      43

Cuts at:   6650    7647 
   Size:        997

  Fragments arranged by size: 

                3064     997     979     717     604     518     438     287
                  43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2083    2293    4834    7647 
   Size:       2083     210    2541    2813

  Fragments arranged by size: 

                2813    2541    2083     210
 
EcoICRI GAG'CTC

Cuts at:      0     129    2568    4008    7647 
   Size:        129    2439    1440    3639

  Fragments arranged by size: 

                3639    2439    1440     129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115    1534    2347    2467    2635    3395    3886    3895 
   Size:        115    1419     813     120     168     760     491       9

Cuts at:   3895    4073    4225    4348    4816    5683    6004    6172    6214 
   Size:        178     152     123     468     867     321     168      42

Cuts at:   6214    7305    7647 
   Size:       1091     342

  Fragments arranged by size: 

                1419    1091     867     813     760     491     468     342
                 321     178     168     168     152     123     120     115
                  42       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1720    2062    2971    3796    3919    6094    7647 
   Size:       1720     342     909     825     123    2175    1553

  Fragments arranged by size: 

                2175    1720    1553     909     825     342     123
 
EcoO109I rG'GnC_Cy

Cuts at:      0     597    1037    1677    3757    3912    4107    4108    4219 
   Size:        597     440     640    2080     155     195       1     111

Cuts at:   4219    4662    4704    5910    6180    6181    6285    6303    6402 
   Size:        443      42    1206     270       1     104      18      99

Cuts at:   6402    6503    7473    7647 
   Size:        101     970     174

  Fragments arranged by size: 

                2080    1206     970     640     597     443     440     270
                 195     174     155     111     104     101      99      42
                  18       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3118    4084    7647 
   Size:       3118     966    3563

  Fragments arranged by size: 

                3563    3118     966
 
EcoRV GAT'ATC

Cuts at:      0    1500    7647 
   Size:       1500    6147
 
FspI TGC'GCA

Cuts at:      0    2191    7647 
   Size:       2191    5456
 
HaeII r_GCGC'y

Cuts at:      0    2046    3314    3406    3624    3812    5333    7647 
   Size:       2046    1268      92     218     188    1521    2314

  Fragments arranged by size: 

                2314    2046    1521    1268     218     188      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1980    2012    2636    2668    3439    3471    3514    3546 
   Size:       1980      32     624      32     771      32      43      32

Cuts at:   3546    3593    3625    3871    3903    4654    4686    4734    4766 
   Size:         47      32     246      32     751      32      48      32

Cuts at:   4766    4823    4826    4855    4858    5602    5634    6044    6076 
   Size:         57       3      29       3     744      32     410      32

Cuts at:   6076    6625    6657    6662    6694    7330    7362    7647 
   Size:        549      32       5      32     636      32     285

  Fragments arranged by size: 

                1980     771     751     744     636     624     549     410
                 285     246      57      48      47      43      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      29       5       3       3
 
HincII GTy'rAC

Cuts at:      0    2808    4474    6615    7148    7647 
   Size:       2808    1666    2141     533     499

  Fragments arranged by size: 

                2808    2141    1666     533     499
 
HpaI GTT'AAC

Cuts at:      0    6615    7647 
   Size:       6615    1032
 
KasI G'GCGC_C

Cuts at:      0    3310    7647 
   Size:       3310    4337
 
KpnI G_GTAC'C

Cuts at:      0    5901    7647 
   Size:       5901    1746
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1230    2201    6348    7049    7588    7647 
   Size:       1230     971    4147     701     539      59

  Fragments arranged by size: 

                4147    1230     971     701     539      59
 
MscI TGG'CCA

Cuts at:      0     265     526     748    1916    2131    2503    2545    3719 
   Size:        265     261     222    1168     215     372      42    1174

Cuts at:   3719    3940    5748    6058    6628    6898    7355    7647 
   Size:        221    1808     310     570     270     457     292

  Fragments arranged by size: 

                1808    1174    1168     570     457     372     310     292
                 270     265     261     222     221     215      42
 
MslI CAynn'nnrTG

Cuts at:      0     283     370     583    1066    1459    1555    1922    2476 
   Size:        283      87     213     483     393      96     367     554

Cuts at:   2476    2656    2710    3109    3517    3577    4600    4683    6031 
   Size:        180      54     399     408      60    1023      83    1348

Cuts at:   6031    6982    7207    7246    7647 
   Size:        951     225      39     401

  Fragments arranged by size: 

                1348    1023     951     554     483     408     401     399
                 393     367     283     225     213     180      96      87
                  83      60      54      39
 
MspA1I CmG'CkG

Cuts at:      0      34     424     502     879    1294    1692    2845    3045 
   Size:         34     390      78     377     415     398    1153     200

Cuts at:   3045    3219    3303    3549    4285    4555    5274    5354    5462 
   Size:        174      84     246     736     270     719      80     108

Cuts at:   5462    5592    6342    6883    7647 
   Size:        130     750     541     764

  Fragments arranged by size: 

                1153     764     750     736     719     541     415     398
                 390     377     270     246     200     174     130     108
                  84      80      78      34
 
NarI GG'CG_CC

Cuts at:      0    3311    7647 
   Size:       3311    4336
 
NcoI C'CATG_G

Cuts at:      0     146     401     783    2852    2906    3518    4766    7647 
   Size:        146     255     382    2069      54     612    1248    2881

  Fragments arranged by size: 

                2881    2069    1248     612     382     255     146      54
            
 
NdeI CA'TA_TG

Cuts at:      0    4176    4758    6429    7647 
   Size:       4176     582    1671    1218

  Fragments arranged by size: 

                4176    1671    1218     582
 
NheI G'CTAG_C

Cuts at:      0     271    7647 
   Size:        271    7376
 
NsiI A_TGCA'T

Cuts at:      0    3251    3732    7647 
   Size:       3251     481    3915

  Fragments arranged by size: 

                3915    3251     481
 
NspI r_CATG'y

Cuts at:      0     499    1464    1671    2268    2481    3234    4443    4785 
   Size:        499     965     207     597     213     753    1209     342

Cuts at:   4785    5112    5151    5191    5374    5994    6141    6166    6868 
   Size:        327      39      40     183     620     147      25     702

Cuts at:   6868    7199    7647 
   Size:        331     448

  Fragments arranged by size: 

                1209     965     753     702     620     597     499     448
                 342     331     327     213     207     183     147      40
                  39      25
 
PasI CC'CwG_GG

Cuts at:      0    1308    2529    2943    7647 
   Size:       1308    1221     414    4704

  Fragments arranged by size: 

                4704    1308    1221     414
 
PciI A'CATG_T

Cuts at:      0    5108    5990    7647 
   Size:       5108     882    1657

  Fragments arranged by size: 

                5108    1657     882
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1308    1692    3045    6065    7647 
   Size:       1308     384    1353    3020    1582

  Fragments arranged by size: 

                3020    1582    1353    1308     384
 
PfoI T'CCnGG_A

Cuts at:      0    2120    2483    3023    3457    5699    6749    7647 
   Size:       2120     363     540     434    2242    1050     898

  Fragments arranged by size: 

                2242    2120    1050     898     540     434     363
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     912     944    7647 
   Size:        912      32    6703

  Fragments arranged by size: 

                6703     912      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     597    1037    3757    4662    4704    6285    6303    6402 
   Size:        597     440    2720     905      42    1581      18      99

Cuts at:   6402    7647 
   Size:       1245

  Fragments arranged by size: 

                2720    1581    1245     905     597     440      99      42
                  18
 
PshAI GACnn'nnGTC

Cuts at:      0    5891    7647 
   Size:       5891    1756
 
PspOMI G'GGCC_C

Cuts at:      0    3911    4107    6180    7647 
   Size:       3911     196    2073    1467

  Fragments arranged by size: 

                3911    2073    1467     196
 
PspXI vC'TCGA_Gb

Cuts at:      0    1469    4450    7647 
   Size:       1469    2981    3197

  Fragments arranged by size: 

                3197    2981    1469
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6942    6974    7647 
   Size:       6942      32     673

  Fragments arranged by size: 

                6942     673      32
 
PstI C_TGCA'G

Cuts at:      0      68    1557    3643    4554    4871    5321    5597    7647 
   Size:         68    1489    2086     911     317     450     276    2050

  Fragments arranged by size: 

                2086    2050    1489     911     450     317     276      68
            
 
PvuII CAG'CTG

Cuts at:      0     502     879    1692    2845    3045    3219    3303    3549 
   Size:        502     377     813    1153     200     174      84     246

Cuts at:   3549    4555    5274    5354    6342    6883    7647 
   Size:       1006     719      80     988     541     764

  Fragments arranged by size: 

                1153    1006     988     813     764     719     541     502
                 377     246     200     174      84      80
 
SacI G_AGCT'C

Cuts at:      0     131    2570    4010    7647 
   Size:        131    2439    1440    3637

  Fragments arranged by size: 

                3637    2439    1440     131
 
SanDI GG'GwC_CC

Cuts at:      0     597    7647 
   Size:        597    7050
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3064    6607    7647 
   Size:       3064    3543    1040

  Fragments arranged by size: 

                3543    3064    1040
 
ScaI AGT'ACT

Cuts at:      0     895    5323    7647 
   Size:        895    4428    2324

  Fragments arranged by size: 

                4428    2324     895
 
SexAI A'CCwGG_T

Cuts at:      0    2105    2276    4342    7647 
   Size:       2105     171    2066    3305

  Fragments arranged by size: 

                3305    2105    2066     171
 
SfcI C'TryA_G

Cuts at:      0      64     626    1553    2571    2784    3639    4267    4550 
   Size:         64     562     927    1018     213     855     628     283

Cuts at:   4550    4867    5281    5317    5325    5593    5806    6756    7185 
   Size:        317     414      36       8     268     213     950     429

Cuts at:   7185    7647 
   Size:        462

  Fragments arranged by size: 

                1018     950     927     855     628     562     462     429
                 414     317     283     268     213     213      64      36
                   8
 
SfoI GGC'GCC

Cuts at:      0    3312    7647 
   Size:       3312    4335
 
SmaI CCC'GGG

Cuts at:      0    2728    7647 
   Size:       2728    4919
 
SmlI C'TyrA_G

Cuts at:      0    1469    2593    4450    4960    7647 
   Size:       1469    1124    1857     510    2687

  Fragments arranged by size: 

                2687    1857    1469    1124     510
 
SpeI A'CTAG_T

Cuts at:      0    2791    7647 
   Size:       2791    4856
 
SphI G_CATG'C

Cuts at:      0    1464    1671    2268    4443    5191    5374    6166    6868 
   Size:       1464     207     597    2175     748     183     792     702

Cuts at:   6868    7647 
   Size:        779

  Fragments arranged by size: 

                2175    1464     792     779     748     702     597     207
                 183
 
StuI AGG'CCT

Cuts at:      0    1022    2083    3802    3925    4456    5365    5764    6089 
   Size:       1022    1061    1719     123     531     909     399     325

Cuts at:   6089    6217    7004    7172    7647 
   Size:        128     787     168     475

  Fragments arranged by size: 

                1719    1061    1022     909     787     531     475     399
                 325     168     128     123
 
StyI C'CwwG_G

Cuts at:      0     122     146     380     401     743     783     986    1017 
   Size:        122      24     234      21     342      40     203      31

Cuts at:   1017    1673    2852    2906    3518    3720    4457    4673    4766 
   Size:        656    1179      54     612     202     737     216      93

Cuts at:   4766    5852    7647 
   Size:       1086    1795

  Fragments arranged by size: 

                1795    1179    1086     737     656     612     342     234
                 216     203     202     122      93      54      40      31
                  24      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     457    1116    1676    4138    4633    6307    6959    7034 
   Size:        457     659     560    2462     495    1674     652      75

Cuts at:   7034    7647 
   Size:        613

  Fragments arranged by size: 

                2462    1674     659     652     613     560     495     457
                  75
 
TatI w'GTAC_w

Cuts at:      0     893     963    4811    5321    5636    6110    6260    7647 
   Size:        893      70    3848     510     315     474     150    1387

  Fragments arranged by size: 

                3848    1387     893     510     474     315     150      70
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1063    1095    1883    1915    2266    2298    2525    2557 
   Size:       1063      32     788      32     351      32     227      32

Cuts at:   2557    3041    3073    4771    4803    7647 
   Size:        484      32    1698      32    2844

  Fragments arranged by size: 

                2844    1698    1063     788     484     351     227      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3429    7237    7647 
   Size:       3429    3808     410

  Fragments arranged by size: 

                3808    3429     410
 
XbaI T'CTAG_A

Cuts at:      0    4236    7647 
   Size:       4236    3411
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1527    1909    2139    2511    4707    5853    7032    7624 
   Size:       1527     382     230     372    2196    1146    1179     592

Cuts at:   7624    7647 
   Size:         23

  Fragments arranged by size: 

                2196    1527    1179    1146     592     382     372     230
                  23
 
XhoI C'TCGA_G

Cuts at:      0    1469    4450    7647 
   Size:       1469    2981    3197

  Fragments arranged by size: 

                3197    2981    1469
 
XmaI C'CCGG_G

Cuts at:      0    2726    7647 
   Size:       2726    4921
 
XmnI GAAnn'nnTTC

Cuts at:      0    7433    7647 
   Size:       7433     214
 
ZraI GAC'GTC

Cuts at:      0    5863    7647 
   Size:       5863    1784
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AseI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
    SanDI     SapI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BsiWI   BssHII  BstZ17I     DraI     FalI     FseI    FspAI  HindIII
     MfeI     MluI     NaeI   NgoMIV     NotI     NruI     PacI     PmeI
     PmlI     PsiI     PvuI    RsrII    SacII     SalI     SbfI     SfiI
     SgfI    SgrAI    SnaBI     SrfI     SspI     SwaI
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