Restriction map for pF1KB0356
 (Linear) (Six-base) MAPSORT of: KIBB0356.seq  Check: 3747  from: 1  to: 3204



KIBB0356.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0     619    3204 
   Size:        619    2585
 
AclI AA'CG_TT

Cuts at:      0     434    3204 
   Size:        434    2770
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1981    3204 
   Size:        686    1295    1223

  Fragments arranged by size: 

                1295    1223     686
 
AflIII A'CryG_T

Cuts at:      0    2692    2850    3204 
   Size:       2692     158     354

  Fragments arranged by size: 

                2692     354     158
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2364    3204 
   Size:       2364     840
 
AleI CACnn'nnGTG

Cuts at:      0    1670    3204 
   Size:       1670    1534
 
ApaLI G'TGCA_C

Cuts at:      0     995    2603    3204 
   Size:        995    1608     601

  Fragments arranged by size: 

                1608     995     601
 
ApoI r'AATT_y

Cuts at:      0     241     379    1010    1688    1722    2556    2576    2830 
   Size:        241     138     631     678      34     834      20     254

Cuts at:   2830    2926    3204 
   Size:         96     278

  Fragments arranged by size: 

                 834     678     631     278     254     241     138      96
                  34      20
 
AseI AT'TA_AT

Cuts at:      0     124    3204 
   Size:        124    3080
 
AvaI C'yCGr_G

Cuts at:      0    1502    3204 
   Size:       1502    1702
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1292    1325    3204 
   Size:       1292      33    1879

  Fragments arranged by size: 

                1879    1292      33
 
BanII G_rGCy'C

Cuts at:      0    2904    3204 
   Size:       2904     300
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1509    2701    3204 
   Size:       1509    1192     503

  Fragments arranged by size: 

                1509    1192     503
 
BfrBI ATG'CAT

Cuts at:      0    3196    3204 
   Size:       3196       8
 
BglII A'GATC_T

Cuts at:      0    1058    3204 
   Size:       1058    2146
 
Bme1580I G_kGCm'C

Cuts at:      0     999    1994    2607    3204 
   Size:        999     995     613     597

  Fragments arranged by size: 

                 999     995     613     597
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1633    3204 
   Size:       1633    1571
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    3204 
   Size:        206    2998
 
BsaAI yAC'GTr

Cuts at:      0    1029    3204 
   Size:       1029    2175
 
BsaBI GATnn'nnATC

Cuts at:      0    3137    3204 
   Size:       3137      67
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1103    2249    3204 
   Size:       1103    1146     955

  Fragments arranged by size: 

                1146    1103     955
 
BsiHKAI G_wGCw'C

Cuts at:      0     999    2607    3204 
   Size:        999    1608     597

  Fragments arranged by size: 

                1608     999     597
 
BsmI GAATG_Cn'

Cuts at:      0     109    2914    3032    3204 
   Size:        109    2805     118     172

  Fragments arranged by size: 

                2805     172     118     109
 
Bsp1286I G_dGCh'C

Cuts at:      0     999    1994    2607    2904    3204 
   Size:        999     995     613     297     300

  Fragments arranged by size: 

                 999     995     613     300     297
 
BspHI T'CATG_A

Cuts at:      0    3116    3204 
   Size:       3116      88
 
BsrDI GCAATG_nn'

Cuts at:      0     494    2129    3053    3204 
   Size:        494    1635     924     151

  Fragments arranged by size: 

                1635     924     494     151
 
BssSI C'ACGA_G

Cuts at:      0    1190    1607    3204 
   Size:       1190     417    1597

  Fragments arranged by size: 

                1597    1190     417
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2430    3204 
   Size:       2430     774
 
BstBI TT'CG_AA

Cuts at:      0    1068    2493    3066    3204 
   Size:       1068    1425     573     138

  Fragments arranged by size: 

                1425    1068     573     138
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1268    2050    3204 
   Size:       1268     782    1154

  Fragments arranged by size: 

                1268    1154     782
 
BstYI r'GATC_y

Cuts at:      0     332     396     453    1058    1611    1821    3170    3204 
   Size:        332      64      57     605     553     210    1349      34

  Fragments arranged by size: 

                1349     605     553     332     210      64      57      34
            
 
BstZ17I GTA'TAC

Cuts at:      0     620    3204 
   Size:        620    2584
 
Bsu36I CC'TnA_GG

Cuts at:      0    2290    3204 
   Size:       2290     914
 
BtgI C'CryG_G

Cuts at:      0    1083    3204 
   Size:       1083    2121
 
BtsI GCAGTG_nn'

Cuts at:      0    1449    2605    3204 
   Size:       1449    1156     599

  Fragments arranged by size: 

                1449    1156     599
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2321    2356    3204 
   Size:       2321      35     848

  Fragments arranged by size: 

                2321     848      35
 
DraI TTT'AAA

Cuts at:      0     150     296     755    2012    2403    3204 
   Size:        150     146     459    1257     391     801

  Fragments arranged by size: 

                1257     801     459     391     150     146
 
EaeI y'GGCC_r

Cuts at:      0    1336    3204 
   Size:       1336    1868
 
EarI CTCTTCn'nnn_

Cuts at:      0    1472    2294    2995    3204 
   Size:       1472     822     701     209

  Fragments arranged by size: 

                1472     822     701     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1981    3204 
   Size:        686    1295    1223

  Fragments arranged by size: 

                1295    1223     686
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     112    3204 
   Size:        112    3092
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462    1911    3204 
   Size:        462    1449    1293

  Fragments arranged by size: 

                1449    1293     462
 
EcoRI G'AATT_C

Cuts at:      0    1722    3204 
   Size:       1722    1482
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     892    1145    3204 
   Size:        892     253    2059

  Fragments arranged by size: 

                2059     892     253
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     324     356    1603    1635    2350    2382    3204 
   Size:        324      32    1247      32     715      32     822

  Fragments arranged by size: 

                1247     822     715     324      32      32      32
 
MfeI C'AATT_G

Cuts at:      0    1488    3204 
   Size:       1488    1716
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1403    3204 
   Size:       1403    1801
 
MscI TGG'CCA

Cuts at:      0    1338    3204 
   Size:       1338    1866
 
MslI CAynn'nnrTG

Cuts at:      0     370     641    1266    1670    2569    2697    2764    3143 
   Size:        370     271     625     404     899     128      67     379

Cuts at:   3143    3204 
   Size:         61

  Fragments arranged by size: 

                 899     625     404     379     370     271     128      67
                  61
 
NcoI C'CATG_G

Cuts at:      0    1083    3204 
   Size:       1083    2121
 
NsiI A_TGCA'T

Cuts at:      0    3198    3204 
   Size:       3198       6
 
NspI r_CATG'y

Cuts at:      0     369    1486    2084    2983    3196    3204 
   Size:        369    1117     598     899     213       8

  Fragments arranged by size: 

                1117     899     598     369     213       8
 
PpuMI rG'GwC_Cy

Cuts at:      0     462    1911    3204 
   Size:        462    1449    1293

  Fragments arranged by size: 

                1449    1293     462
 
PsiI TTA'TAA

Cuts at:      0     988    2840    3204 
   Size:        988    1852     364

  Fragments arranged by size: 

                1852     988     364
 
PstI C_TGCA'G

Cuts at:      0    2612    3204 
   Size:       2612     592
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1472    3204 
   Size:       1472    1732
 
SfcI C'TryA_G

Cuts at:      0    2242    2608    3204 
   Size:       2242     366     596

  Fragments arranged by size: 

                2242     596     366
 
SmlI C'TyrA_G

Cuts at:      0     221    3204 
   Size:        221    2983
 
SphI G_CATG'C

Cuts at:      0     369    1486    2983    3196    3204 
   Size:        369    1117    1497     213       8

  Fragments arranged by size: 

                1497    1117     369     213       8
 
SspI AAT'ATT

Cuts at:      0     426    1041    1864    1930    3204 
   Size:        426     615     823      66    1274

  Fragments arranged by size: 

                1274     823     615     426      66
 
StyI C'CwwG_G

Cuts at:      0    1083    3204 
   Size:       1083    2121
 
TatI w'GTAC_w

Cuts at:      0     405    1905    3204 
   Size:        405    1500    1299

  Fragments arranged by size: 

                1500    1299     405
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      77     109    3204 
   Size:         77      32    3095

  Fragments arranged by size: 

                3095      77      32
 
XbaI T'CTAG_A

Cuts at:      0    1726    3204 
   Size:       1726    1478
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AhdI     AleI    ApaLI     ApoI
     AseI     AvaI     BaeI    BanII    BciVI    BfrBI    BglII Bme1580I
     BplI    BpuEI    BsaAI    BsaBI    BseRI  BsiHKAI     BsmI Bsp1286I
    BspHI    BsrDI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI    CspCI     DraI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I     MfeI     MmeI     MscI
     MslI     NcoI     NsiI     NspI    PpuMI     PsiI     PstI     SapI
     SfcI     SmlI     SphI     SspI     StyI     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AlfI     AloI
    AlwNI     ApaI     AscI    AsiSI    AvrII    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstEII    BtgZI     ClaI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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