Restriction map for pF1KB0179
 (Linear) (Six-base) MAPSORT of: KIBB0179.seq  Check: 4701  from: 1  to: 3660



KIBB0179.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3381    3660 
   Size:       3381     279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1157    1729    3134    3660 
   Size:        364     793     572    1405     526

  Fragments arranged by size: 

                1405     793     572     526     364
 
AleI CACnn'nnGTG

Cuts at:      0    2083    2746    3660 
   Size:       2083     663     914

  Fragments arranged by size: 

                2083     914     663
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     506    3660 
   Size:        506    3154
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2393    2425    3660 
   Size:       2393      32    1235

  Fragments arranged by size: 

                2393    1235      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1749    1774    1803    3660 
   Size:       1749      25      29    1857

  Fragments arranged by size: 

                1857    1749      29      25
 
ApaI G_GGCC'C

Cuts at:      0    2898    3225    3660 
   Size:       2898     327     435

  Fragments arranged by size: 

                2898     435     327
 
ApaLI G'TGCA_C

Cuts at:      0     883    3660 
   Size:        883    2777
 
ApoI r'AATT_y

Cuts at:      0     151     226    1243    3100    3660 
   Size:        151      75    1017    1857     560

  Fragments arranged by size: 

                1857    1017     560     151      75
 
AvaI C'yCGr_G

Cuts at:      0      73     635    3641    3660 
   Size:         73     562    3006      19

  Fragments arranged by size: 

                3006     562      73      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      61      94    3008    3041    3660 
   Size:         61      33    2914      33     619

  Fragments arranged by size: 

                2914     619      61      33      33
 
BanII G_rGCy'C

Cuts at:      0     785     899    1381    2898    3225    3660 
   Size:        785     114     482    1517     327     435

  Fragments arranged by size: 

                1517     785     482     435     327     114
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2325    3042    3660 
   Size:       2325     717     618

  Fragments arranged by size: 

                2325     717     618
 
BbvCI CC'TCA_GC

Cuts at:      0    2148    3660 
   Size:       2148    1512
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1853    1887    2513    2547    3660 
   Size:       1853      34     626      34    1113

  Fragments arranged by size: 

                1853    1113     626      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3036    3660 
   Size:       3036     624
 
BclI T'GATC_A

Cuts at:      0     158    2861    3660 
   Size:        158    2703     799

  Fragments arranged by size: 

                2703     799     158
 
BglII A'GATC_T

Cuts at:      0    1032    3660 
   Size:       1032    2628
 
BlpI GC'TnA_GC

Cuts at:      0    3565    3660 
   Size:       3565      95
 
Bme1580I G_kGCm'C

Cuts at:      0     887    1355    2898    3225    3420    3660 
   Size:        887     468    1543     327     195     240

  Fragments arranged by size: 

                1543     887     468     327     240     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     352     989    1533    1791    2425    2444    2899    3660 
   Size:        352     637     544     258     634      19     455     761

  Fragments arranged by size: 

                 761     637     634     544     455     352     258      19
            
 
BmtI G_CTAG'C

Cuts at:      0    3139    3660 
   Size:       3139     521
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1101    1167    3656    3660 
   Size:       1101      66    2489       4

  Fragments arranged by size: 

                2489    1101      66       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     758    1046    2974    3651    3660 
   Size:        266     492     288    1928     677       9

  Fragments arranged by size: 

                1928     677     492     288     266       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     997    1911    2148    2645    3660 
   Size:        997     914     237     497    1015

  Fragments arranged by size: 

                1015     997     914     497     237
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1330    1696    2779    3660 
   Size:       1330     366    1083     881

  Fragments arranged by size: 

                1330    1083     881     366
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     581    1063    1075    3552    3660 
   Size:        581     482      12    2477     108

  Fragments arranged by size: 

                2477     581     482     108      12
 
BsaBI GATnn'nnATC

Cuts at:      0    1013    1669    3660 
   Size:       1013     656    1991

  Fragments arranged by size: 

                1991    1013     656
 
BsaHI Gr'CG_yC

Cuts at:      0    2629    3660 
   Size:       2629    1031
 
BsaWI w'CCGG_w

Cuts at:      0     372     701    3371    3660 
   Size:        372     329    2670     289

  Fragments arranged by size: 

                2670     372     329     289
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     666     672     696     702    2866    2896    3154    3184 
   Size:        666       6      24       6    2164      30     258      30

Cuts at:   3184    3660 
   Size:        476

  Fragments arranged by size: 

                2164     666     476     258      30      30      24       6
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     761    1093    1909    3660 
   Size:        761     332     816    1751

  Fragments arranged by size: 

                1751     816     761     332
 
BseYI C'CCAG_C

Cuts at:      0     460    1475    1847    2478    2671    3531    3660 
   Size:        460    1015     372     631     193     860     129

  Fragments arranged by size: 

                1015     860     631     460     372     193     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     865    1935    3061    3328    3660 
   Size:        242     623    1070    1126     267     332

  Fragments arranged by size: 

                1126    1070     623     332     267     242
 
BsiHKAI G_wGCw'C

Cuts at:      0     887     899    1134    2090    3467    3660 
   Size:        887      12     235     956    1377     193

  Fragments arranged by size: 

                1377     956     887     235     193      12
 
BsmI GAATG_Cn'

Cuts at:      0    1271    3660 
   Size:       1271    2389
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     265    3660 
   Size:        265    3395
 
Bsp1286I G_dGCh'C

Cuts at:      0     785     887     899    1134    1355    1381    2090    2898 
   Size:        785     102      12     235     221      26     709     808

Cuts at:   2898    3225    3420    3467    3660 
   Size:        327     195      47     193

  Fragments arranged by size: 

                 808     785     709     327     235     221     195     193
                 102      47      26      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3381    3660 
   Size:       3381     279
 
BsrBI CCG'CTC

Cuts at:      0     614     729    3660 
   Size:        614     115    2931

  Fragments arranged by size: 

                2931     614     115
 
BsrDI GCAATG_nn'

Cuts at:      0    1433    3660 
   Size:       1433    2227
 
BsrGI T'GTAC_A

Cuts at:      0    2240    2705    2882    3143    3602    3660 
   Size:       2240     465     177     261     459      58

  Fragments arranged by size: 

                2240     465     459     261     177      58
 
BssSI C'ACGA_G

Cuts at:      0    2620    3660 
   Size:       2620    1040
 
BstEII G'GTnAC_C

Cuts at:      0    3367    3660 
   Size:       3367     293
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1196    1860    3660 
   Size:       1196     664    1800

  Fragments arranged by size: 

                1800    1196     664
 
BstYI r'GATC_y

Cuts at:      0     306    1032    1745    1771    2372    3660 
   Size:        306     726     713      26     601    1288

  Fragments arranged by size: 

                1288     726     713     601     306      26
 
Bsu36I CC'TnA_GG

Cuts at:      0    1280    3660 
   Size:       1280    2380
 
BtgI C'CryG_G

Cuts at:      0      17      53    1505    2801    2870    3178    3660 
   Size:         17      36    1452    1296      69     308     482

  Fragments arranged by size: 

                1452    1296     482     308      69      36      17
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2516    3660 
   Size:       2516    1144
 
BtsI GCAGTG_nn'

Cuts at:      0    2171    3009    3330    3660 
   Size:       2171     838     321     330

  Fragments arranged by size: 

                2171     838     330     321
 
DraIII CAC_nnn'GTG

Cuts at:      0    2320    3660 
   Size:       2320    1340
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1665    3660 
   Size:       1665    1995
 
EaeI y'GGCC_r

Cuts at:      0     917    1445    1877    3660 
   Size:        917     528     432    1783

  Fragments arranged by size: 

                1783     917     528     432
 
EarI CTCTTCn'nnn_

Cuts at:      0     358     773    1256    1325    1851    2450    3427    3660 
   Size:        358     415     483      69     526     599     977     233

  Fragments arranged by size: 

                 977     599     526     483     415     358     233      69
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1822    3660 
   Size:       1822    1838
 
EcoICRI GAG'CTC

Cuts at:      0     897    3660 
   Size:        897    2763
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     364     758    1046    1157    1729    2974    3134 
   Size:        266      98     394     288     111     572    1245     160

Cuts at:   3134    3651    3660 
   Size:        517       9

  Fragments arranged by size: 

                1245     572     517     394     288     266     160     111
                  98       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1807    3660 
   Size:       1807    1853
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49     141     632    1305    2401    2894    2895    2910 
   Size:         49      92     491     673    1096     493       1      15

Cuts at:   2910    3660 
   Size:        750

  Fragments arranged by size: 

                1096     750     673     493     491      92      49      15
                   1
 
EcoRV GAT'ATC

Cuts at:      0     664    2325    3660 
   Size:        664    1661    1335

  Fragments arranged by size: 

                1661    1335     664
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     369    1641    3660 
   Size:        369    1272    2019

  Fragments arranged by size: 

                2019    1272     369
 
HaeII r_GCGC'y

Cuts at:      0    1170    3660 
   Size:       1170    2490
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      17      49      67      99     666     672     698     704 
   Size:         17      32      18      32     567       6      26       6

Cuts at:    704    1756    1788    2250    2282    2806    2838    3660 
   Size:       1052      32     462      32     524      32     822

  Fragments arranged by size: 

                1052     822     567     524     462      32      32      32
                  32      32      26      18      17       6       6
 
HincII GTy'rAC

Cuts at:      0    1530    3660 
   Size:       1530    2130
 
HindIII A'AGCT_T

Cuts at:      0     449    3660 
   Size:        449    3211
 
MfeI C'AATT_G

Cuts at:      0    1717    3660 
   Size:       1717    1943
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     380    2030    3197    3660 
   Size:         20     360    1650    1167     463

  Fragments arranged by size: 

                1650    1167     463     360      20
 
MscI TGG'CCA

Cuts at:      0     919    1447    1879    3660 
   Size:        919     528     432    1781

  Fragments arranged by size: 

                1781     919     528     432
 
MslI CAynn'nnrTG

Cuts at:      0    1372    2081    2083    2746    2869    2986    3660 
   Size:       1372     709       2     663     123     117     674

  Fragments arranged by size: 

                1372     709     674     663     123     117       2
 
MspA1I CmG'CkG

Cuts at:      0      55     849    1479    2880    3660 
   Size:         55     794     630    1401     780

  Fragments arranged by size: 

                1401     794     780     630      55
 
NcoI C'CATG_G

Cuts at:      0      17    1505    2801    2870    3178    3660 
   Size:         17    1488    1296      69     308     482

  Fragments arranged by size: 

                1488    1296     482     308      69      17
 
NheI G'CTAG_C

Cuts at:      0    3135    3660 
   Size:       3135     525
 
NspI r_CATG'y

Cuts at:      0     760    1624    3486    3660 
   Size:        760     864    1862     174

  Fragments arranged by size: 

                1862     864     760     174
 
PasI CC'CwG_GG

Cuts at:      0    2397    2686    3660 
   Size:       2397     289     974

  Fragments arranged by size: 

                2397     974     289
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1803    1923    2622    3179    3393    3459    3629    3660 
   Size:       1803     120     699     557     214      66     170      31

  Fragments arranged by size: 

                1803     699     557     214     170     120      66      31
            
 
PfoI T'CCnGG_A

Cuts at:      0     628     670    3151    3660 
   Size:        628      42    2481     509

  Fragments arranged by size: 

                2481     628     509      42
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     632    1305    2401    3660 
   Size:        141     491     673    1096    1259

  Fragments arranged by size: 

                1259    1096     673     491     141
 
PsiI TTA'TAA

Cuts at:      0     803    3660 
   Size:        803    2857
 
PspOMI G'GGCC_C

Cuts at:      0    2894    3221    3660 
   Size:       2894     327     439

  Fragments arranged by size: 

                2894     439     327
 
PspXI vC'TCGA_Gb

Cuts at:      0      73    3660 
   Size:         73    3587
 
PstI C_TGCA'G

Cuts at:      0    3004    3325    3660 
   Size:       3004     321     335

  Fragments arranged by size: 

                3004     335     321
 
PvuII CAG'CTG

Cuts at:      0     849    2880    3660 
   Size:        849    2031     780

  Fragments arranged by size: 

                2031     849     780
 
SacI G_AGCT'C

Cuts at:      0     899    3660 
   Size:        899    2761
 
SacII CC_GC'GG

Cuts at:      0      56    3660 
   Size:         56    3604
 
SapI GCTCTTCn'nnn_

Cuts at:      0     773    1325    2450    3660 
   Size:        773     552    1125    1210

  Fragments arranged by size: 

                1210    1125     773     552
 
SexAI A'CCwGG_T

Cuts at:      0    2316    3660 
   Size:       2316    1344
 
SfcI C'TryA_G

Cuts at:      0      11    3000    3054    3321    3660 
   Size:         11    2989      54     267     339

  Fragments arranged by size: 

                2989     339     267      54      11
 
SmaI CCC'GGG

Cuts at:      0     637    3660 
   Size:        637    3023
 
SmlI C'TyrA_G

Cuts at:      0      73    1309    1675    2794    3641    3660 
   Size:         73    1236     366    1119     847      19

  Fragments arranged by size: 

                1236    1119     847     366      73      19
 
SphI G_CATG'C

Cuts at:      0    1624    3660 
   Size:       1624    2036
 
StuI AGG'CCT

Cuts at:      0     878    3660 
   Size:        878    2782
 
StyI C'CwwG_G

Cuts at:      0      17    1442    1505    2156    2801    2870    3062    3178 
   Size:         17    1425      63     651     645      69     192     116

Cuts at:   3178    3473    3660 
   Size:        295     187

  Fragments arranged by size: 

                1425     651     645     295     192     187     116      69
                  63      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     656    1810    1829    3432    3660 
   Size:        656    1154      19    1603     228

  Fragments arranged by size: 

                1603    1154     656     228      19
 
TatI w'GTAC_w

Cuts at:      0    1976    2240    2435    2705    2882    3143    3602    3660 
   Size:       1976     264     195     270     177     261     459      58

  Fragments arranged by size: 

                1976     459     270     264     261     195     177      58
            
 
XbaI T'CTAG_A

Cuts at:      0     658    3660 
   Size:        658    3002
 
XhoI C'TCGA_G

Cuts at:      0      73    3641    3660 
   Size:         73    3568      19

  Fragments arranged by size: 

                3568      73      19
 
XmaI C'CCGG_G

Cuts at:      0     635    3660 
   Size:        635    3025
 
XmnI GAAnn'nnTTC

Cuts at:      0    1244    3049    3660 
   Size:       1244    1805     611

  Fragments arranged by size: 

                1805    1244     611
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrGI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NheI
     NspI     PasI    PflMI     PfoI    PpuMI     PsiI   PspOMI    PspXI
     PstI    PvuII     SacI    SacII     SapI    SexAI     SfcI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     XbaI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AscI     AseI    AsiSI    AvrII    BamHI     BanI     BbeI
    BfrBI     BglI    BmgBI    BsaAI    BsiEI    BsiWI    BspEI    BspHI
    BsrFI   BssHII   BstAPI    BstBI  BstZ17I     ClaI    CspCI     DraI
     EagI    EcoRI     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsrI     PvuI
    RsrII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I
     XcmI     ZraI
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