Restriction map for pF1KB0174
 (Linear) (Six-base) MAPSORT of: KIBB0174.seq  Check: 5827  from: 1  to: 4074



KIBB0174.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AatII G_ACGT'C

Cuts at:      0    1710    4074 
   Size:       1710    2364
 
AccI GT'mk_AC

Cuts at:      0     252     668    2072    4074 
   Size:        252     416    1404    2002

  Fragments arranged by size: 

                2002    1404     416     252
 
Acc65I G'GTAC_C

Cuts at:      0    3487    4074 
   Size:       3487     587
 
AclI AA'CG_TT

Cuts at:      0     600     900    1003    4074 
   Size:        600     300     103    3071

  Fragments arranged by size: 

                3071     600     300     103
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     955    1325    2332    2815    3914    4074 
   Size:        955     370    1007     483    1099     160

  Fragments arranged by size: 

                1099    1007     955     483     370     160
 
AfeI AGC'GCT

Cuts at:      0     376    4074 
   Size:        376    3698
 
AflIII A'CryG_T

Cuts at:      0    2541    3028    4074 
   Size:       2541     487    1046

  Fragments arranged by size: 

                2541    1046     487
 
AgeI A'CCGG_T

Cuts at:      0    2763    4074 
   Size:       2763    1311
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3378    4074 
   Size:       3378     696
 
AleI CACnn'nnGTG

Cuts at:      0    1689    4074 
   Size:       1689    2385
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2882    2996    4074 
   Size:       2882     114    1078

  Fragments arranged by size: 

                2882    1078     114
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3496    3528    4074 
   Size:       3496      32     546

  Fragments arranged by size: 

                3496     546      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     396    2310    4074 
   Size:        396    1914    1764

  Fragments arranged by size: 

                1914    1764     396
 
ApaI G_GGCC'C

Cuts at:      0    3961    4074 
   Size:       3961     113
 
ApaLI G'TGCA_C

Cuts at:      0    3683    4074 
   Size:       3683     391
 
ApoI r'AATT_y

Cuts at:      0    1885    1963    3263    3908    4074 
   Size:       1885      78    1300     645     166

  Fragments arranged by size: 

                1885    1300     645     166      78
 
AvaI C'yCGr_G

Cuts at:      0     122    4074 
   Size:        122    3952
 
AvrII C'CTAG_G

Cuts at:      0    1291    4074 
   Size:       1291    2783
 
BamHI G'GATC_C

Cuts at:      0     174    2289    4074 
   Size:        174    2115    1785

  Fragments arranged by size: 

                2115    1785     174
 
BanI G'GyrC_C

Cuts at:      0    2020    3487    4074 
   Size:       2020    1467     587

  Fragments arranged by size: 

                2020    1467     587
 
BanII G_rGCy'C

Cuts at:      0    1589    3555    3961    4074 
   Size:       1589    1966     406     113

  Fragments arranged by size: 

                1966    1589     406     113
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     536    2357    3088    3374    4074 
   Size:        536    1821     731     286     700

  Fragments arranged by size: 

                1821     731     700     536     286
 
BbvCI CC'TCA_GC

Cuts at:      0    1880    4074 
   Size:       1880    2194
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2665    2699    4074 
   Size:       2665      34    1375

  Fragments arranged by size: 

                2665    1375      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2003    4074 
   Size:       2003    2071
 
BclI T'GATC_A

Cuts at:      0    2637    4074 
   Size:       2637    1437
 
BfrBI ATG'CAT

Cuts at:      0    3303    4074 
   Size:       3303     771
 
BglII A'GATC_T

Cuts at:      0    3097    4074 
   Size:       3097     977
 
BlpI GC'TnA_GC

Cuts at:      0     746    2882    4074 
   Size:        746    2136    1192

  Fragments arranged by size: 

                2136    1192     746
 
Bme1580I G_kGCm'C

Cuts at:      0    3687    3961    4074 
   Size:       3687     274     113

  Fragments arranged by size: 

                3687     274     113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     981    2489    2666    4074 
   Size:        981    1508     177    1408

  Fragments arranged by size: 

                1508    1408     981     177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     343    4074 
   Size:        343    3731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     446    3338    4074 
   Size:        232     214    2892     736

  Fragments arranged by size: 

                2892     736     232     214
 
Bpu10I CC'TnA_GC

Cuts at:      0    1880    4074 
   Size:       1880    2194
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1332    1831    4074 
   Size:       1332     499    2243

  Fragments arranged by size: 

                2243    1332     499
 
BsaAI yAC'GTr

Cuts at:      0    1780    4074 
   Size:       1780    2294
 
BsaBI GATnn'nnATC

Cuts at:      0    1772    3302    3755    4074 
   Size:       1772    1530     453     319

  Fragments arranged by size: 

                1772    1530     453     319
 
BsaHI Gr'CG_yC

Cuts at:      0    1707    2937    3564    4074 
   Size:       1707    1230     627     510

  Fragments arranged by size: 

                1707    1230     627     510
 
BsaWI w'CCGG_w

Cuts at:      0    1377    2763    4074 
   Size:       1377    1386    1311

  Fragments arranged by size: 

                1386    1377    1311
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3116    3146    4074 
   Size:       3116      30     928

  Fragments arranged by size: 

                3116     928      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     588     710    1827    2657    3704    4074 
   Size:        588     122    1117     830    1047     370

  Fragments arranged by size: 

                1117    1047     830     588     370     122
 
BseYI C'CCAG_C

Cuts at:      0    3368    4074 
   Size:       3368     706
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224    4074 
   Size:        224    3850
 
BsiHKAI G_wGCw'C

Cuts at:      0    1589    3324    3687    4074 
   Size:       1589    1735     363     387

  Fragments arranged by size: 

                1735    1589     387     363
 
Bsp1286I G_dGCh'C

Cuts at:      0    1589    3324    3555    3687    3961    4074 
   Size:       1589    1735     231     132     274     113

  Fragments arranged by size: 

                1735    1589     274     231     132     113
 
BspEI T'CCGG_A

Cuts at:      0    1377    4074 
   Size:       1377    2697
 
BspHI T'CATG_A

Cuts at:      0      69    2378    2430    2967    3984    4074 
   Size:         69    2309      52     537    1017      90

  Fragments arranged by size: 

                2309    1017     537      90      69      52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     721    4074 
   Size:        721    3353
 
BsrDI GCAATG_nn'

Cuts at:      0      20     181     719     945    1284    2871    3396    4074 
   Size:         20     161     538     226     339    1587     525     678

  Fragments arranged by size: 

                1587     678     538     525     339     226     161      20
            
 
BsrFI r'CCGG_y

Cuts at:      0    2763    4074 
   Size:       2763    1311
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     315    4074 
   Size:        315    3759
 
BstBI TT'CG_AA

Cuts at:      0    2805    4074 
   Size:       2805    1269
 
BstEII G'GTnAC_C

Cuts at:      0     465    4074 
   Size:        465    3609
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2412    2933    3205    4074 
   Size:       2412     521     272     869

  Fragments arranged by size: 

                2412     869     521     272
 
BstYI r'GATC_y

Cuts at:      0      35     174     392     648    2289    3097    4074 
   Size:         35     139     218     256    1641     808     977

  Fragments arranged by size: 

                1641     977     808     256     218     139      35
 
Bsu36I CC'TnA_GG

Cuts at:      0    1109    3376    4074 
   Size:       1109    2267     698

  Fragments arranged by size: 

                2267    1109     698
 
BtgI C'CryG_G

Cuts at:      0    2024    3491    3726    3974    4074 
   Size:       2024    1467     235     248     100

  Fragments arranged by size: 

                2024    1467     248     235     100
 
BtsI GCAGTG_nn'

Cuts at:      0    2866    3489    3687    4074 
   Size:       2866     623     198     387

  Fragments arranged by size: 

                2866     623     387     198
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1053    1088    4074 
   Size:       1053      35    2986

  Fragments arranged by size: 

                2986    1053      35
 
DraI TTT'AAA

Cuts at:      0     114    2159    3404    3416    4074 
   Size:        114    2045    1245      12     658

  Fragments arranged by size: 

                2045    1245     658     114      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     700    2270    2339    4074 
   Size:        700    1570      69    1735

  Fragments arranged by size: 

                1735    1570     700      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2270    4074 
   Size:       2270    1804
 
EcoICRI GAG'CTC

Cuts at:      0    1587    4074 
   Size:       1587    2487
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     446     955    1325    2332    2815    3338    3914 
   Size:        232     214     509     370    1007     483     523     576

Cuts at:   3914    4074 
   Size:        160

  Fragments arranged by size: 

                1007     576     523     509     483     370     232     214
                 160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3870    4074 
   Size:       3870     204
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2619    3372    3824    3957    4074 
   Size:       2619     753     452     133     117

  Fragments arranged by size: 

                2619     753     452     133     117
 
EcoRI G'AATT_C

Cuts at:      0    3263    4074 
   Size:       3263     811
 
EcoRV GAT'ATC

Cuts at:      0    1365    4074 
   Size:       1365    2709
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2374    2512    4074 
   Size:       2374     138    1562

  Fragments arranged by size: 

                2374    1562     138
 
HaeII r_GCGC'y

Cuts at:      0     378    4074 
   Size:        378    3696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2076    2108    2962    2994    2998    3030    3116    3148 
   Size:       2076      32     854      32       4      32      86      32

Cuts at:   3148    3364    3396    3403    3435    3653    3685    4074 
   Size:        216      32       7      32     218      32     389

  Fragments arranged by size: 

                2076     854     389     218     216      86      32      32
                  32      32      32      32      32       7       4
 
HincII GTy'rAC

Cuts at:      0    1322    2708    4074 
   Size:       1322    1386    1366

  Fragments arranged by size: 

                1386    1366    1322
 
KpnI G_GTAC'C

Cuts at:      0    3491    4074 
   Size:       3491     583
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    4074 
   Size:        454    3620
 
MslI CAynn'nnrTG

Cuts at:      0    1565    1689    1778    2429    2931    2966    4074 
   Size:       1565     124      89     651     502      35    1108

  Fragments arranged by size: 

                1565    1108     651     502     124      89      35
 
MspA1I CmG'CkG

Cuts at:      0     403    2065    3607    3775    4074 
   Size:        403    1662    1542     168     299

  Fragments arranged by size: 

                1662    1542     403     299     168
 
NcoI C'CATG_G

Cuts at:      0    3491    3726    4074 
   Size:       3491     235     348

  Fragments arranged by size: 

                3491     348     235
 
NsiI A_TGCA'T

Cuts at:      0    3305    4074 
   Size:       3305     769
 
NspI r_CATG'y

Cuts at:      0    2241    2545    2586    2613    3032    4074 
   Size:       2241     304      41      27     419    1042

  Fragments arranged by size: 

                2241    1042     419     304      41      27
 
PciI A'CATG_T

Cuts at:      0    2541    3028    4074 
   Size:       2541     487    1046

  Fragments arranged by size: 

                2541    1046     487
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2006    2929    4074 
   Size:       2006     923    1145

  Fragments arranged by size: 

                2006    1145     923
 
PfoI T'CCnGG_A

Cuts at:      0    1487    3902    4074 
   Size:       1487    2415     172

  Fragments arranged by size: 

                2415    1487     172
 
PpuMI rG'GwC_Cy

Cuts at:      0    3372    3824    4074 
   Size:       3372     452     250

  Fragments arranged by size: 

                3372     452     250
 
PshAI GACnn'nnGTC

Cuts at:      0    2737    4074 
   Size:       2737    1337
 
PsiI TTA'TAA

Cuts at:      0    1144    4074 
   Size:       1144    2930
 
PspOMI G'GGCC_C

Cuts at:      0    3957    4074 
   Size:       3957     117
 
PspXI vC'TCGA_Gb

Cuts at:      0     122    4074 
   Size:        122    3952
 
PstI C_TGCA'G

Cuts at:      0    1017    3750    4074 
   Size:       1017    2733     324

  Fragments arranged by size: 

                2733    1017     324
 
PvuII CAG'CTG

Cuts at:      0     403    2065    3607    3775    4074 
   Size:        403    1662    1542     168     299

  Fragments arranged by size: 

                1662    1542     403     299     168
 
SacI G_AGCT'C

Cuts at:      0    1589    4074 
   Size:       1589    2485
 
ScaI AGT'ACT

Cuts at:      0     102    3846    4074 
   Size:        102    3744     228

  Fragments arranged by size: 

                3744     228     102
 
SfcI C'TryA_G

Cuts at:      0      44    1013    1156    3738    3746    3870    4074 
   Size:         44     969     143    2582       8     124     204

  Fragments arranged by size: 

                2582     969     204     143     124      44       8
 
SmlI C'TyrA_G

Cuts at:      0     122    1311    1846    4074 
   Size:        122    1189     535    2228

  Fragments arranged by size: 

                2228    1189     535     122
 
SpeI A'CTAG_T

Cuts at:      0    3847    4074 
   Size:       3847     227
 
SspI AAT'ATT

Cuts at:      0     956    1371    1650    4074 
   Size:        956     415     279    2424

  Fragments arranged by size: 

                2424     956     415     279
 
StuI AGG'CCT

Cuts at:      0     143    4074 
   Size:        143    3931
 
StyI C'CwwG_G

Cuts at:      0     995    1291    3153    3491    3726    4074 
   Size:        995     296    1862     338     235     348

  Fragments arranged by size: 

                1862     995     348     338     296     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3585    4074 
   Size:       3585     489
 
TatI w'GTAC_w

Cuts at:      0     100    1497    2059    2776    3844    4074 
   Size:        100    1397     562     717    1068     230

  Fragments arranged by size: 

                1397    1068     717     562     230     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     507     539    4074 
   Size:        507      32    3535

  Fragments arranged by size: 

                3535     507      32
 
XbaI T'CTAG_A

Cuts at:      0    3267    4045    4074 
   Size:       3267     778      29

  Fragments arranged by size: 

                3267     778      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2930    3639    4074 
   Size:       2930     709     435

  Fragments arranged by size: 

                2930     709     435
 
XhoI C'TCGA_G

Cuts at:      0     122    4074 
   Size:        122    3952
 
XmnI GAAnn'nnTTC

Cuts at:      0    1004    1202    1385    2259    2374    4074 
   Size:       1004     198     183     874     115    1700

  Fragments arranged by size: 

                1700    1004     874     198     183     115
 
ZraI GAC'GTC

Cuts at:      0    1708    4074 
   Size:       1708    2366
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     DraI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
     KpnI     MmeI     MslI   MspA1I     NcoI     NsiI     NspI     PciI
    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI
    PvuII     SacI     ScaI     SfcI     SmlI     SpeI     SspI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI    AflII     AscI     AseI    AsiSI     BaeI     BbeI     BglI
    BmgBI     BmtI     BsaI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI
    BsrGI   BssHII    BssSI  BstZ17I    BtgZI     ClaI   DraIII     DrdI
     EaeI     EagI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SwaI  Tth111I     XmaI
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