Restriction map for pF1KB0019
##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AccIII      AcyI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AspEI       AviII       
BalI        BamHI       BbeI        BbvCI       BcgI        
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BssHII      BssSI       BstEII      
BstPI       ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       FauI        FseI        FspI        Hin1I       
HpaI        KasI        KspI        MluI        MluNI       
MroI        MscI        NaeI        NarI        NcoI        
NgoMIV      NotI        PacI        PinAI       PmeI        
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        613  GGTACC               G^GTACC
AclI         3434  AACGTT               AA^CGTT
Alw44I       1832  GTGCAC               G^TGCAC
ApaLI        1832  GTGCAC               G^TGCAC
Asp718I       613  GGTACC               G^GTACC
AspI         5242  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        8368  CCTAGG               C^CTAGG
BanII        5971  GAGCCC               G[GA]GC[CT]^C
BbrPI        5360  CACGTG               CAC^GTG
BciVI        7816  GTATCC               GTATCC(6/5)
BglI         1540  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         8368  CCTAGG               C^CTAGG
Bsp1407I     4841  TGTACA               T^GTACA
BsrBI         772  GAGCGG               CCGCTC(-3/-3)
BsrFI         754  ACCGGC               [GA]^CCGG[CT]
BsrGI        4841  TGTACA               T^GTACA
Bst1107I      689  GTATAC               GTA^TAC
BstBI        6707  TTCGAA               TT^CGAA
BstZ17I       689  GTATAC               GTA^TAC
Cfr10I        754  ACCGGC               [GA]^CCGG[CT]
DraIII       8315  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
KpnI          613  GGTACC               GGTAC^C
MfeI          562  CAATTG               C^AATTG
MunI          562  CAATTG               C^AATTG
NheI         1002  GCTAGC               G^CTAGC
NruI          991  TCGCGA               TCG^CGA
NspV         6707  TTCGAA               TT^CGAA
PflFI        5242  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        5360  CACGTG               CAC^GTG
PmlI         5360  CACGTG               CAC^GTG
PshAI        2022  GACAATAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3434  AACGTT               AA^CGTT
SapI         1378  GAAGAGC              GCTCTTC(1/4)
SexAI        5243  ACCAGGT              A^CC[AT]GGT
SfuI         6707  TTCGAA               TT^CGAA
SmaI         1118  CCCGGG               CCC^GGG
SphI         2114  GCATGC               GCATG^C
SspBI        4841  TGTACA               T^GTACA
StuI         2197  AGGCCT               AGG^CCT
SwaI         5393  ATTTAAAT             ATTT^AAAT
Tth111I      5242  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1118  CCCGGG               C^CCGGG
XmaI         1118  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflII        6757  CTTAAG               C^TTAAG
             7353  CTTAAG              
AocI         7073  CCTCAGG              CC^T[ACGT]AGG
             7892  CCTCAGG             
AseI         3361  ATTAAT               AT^TAAT
             5182  ATTAAT              
AsnI         3361  ATTAAT               AT^TAAT
             5182  ATTAAT              
BanI          613  GGTACC               G^G[CT][GA]CC
             7941  GGCACC              
BclI           18  TGATCA               T^GATCA
             6456  TGATCA              
BfrI         6757  CTTAAG               C^TTAAG
             7353  CTTAAG              
BlpI          769  GCTGAGC              GC^T[ACGT]AGC
             2296  GCTCAGC             
Bpu1102I      769  GCTGAGC              GC^T[ACGT]AGC
             2296  GCTCAGC             
BsaAI        4763  CACGTA               [CT]AC^GT[GA]
             5360  CACGTG              
BsaBI         302  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6902  GATCAGGATC          
BsaWI        1865  ACCGGA               [AT]^CCGG[AT]
             5877  TCCGGT              
BsmI         5176  GAATGC               GAATGC(1/-1)
             7158  GAATGC              
BspHI         972  TCATGA               T^CATGA
             1278  TCATGA              
BspLU11I     2836  ACATGT               A^CATGT
             7770  ACATGT              
Bsu36I       7073  CCTCAGG              CC^T[ACGT]AGG
             7892  CCTCAGG             
CelII         769  GCTGAGC              GC^T[ACGT]AGC
             2296  GCTCAGC             
DraII        4682  AGGACCT              [GA]G^G[ACGT]CC[CT]
             7426  AGGTCCC             
EaeI         6972  TGGCCG               [CT]^GGCC[GA]
             8401  CGGCCA              
Eco81I       7073  CCTCAGG              CC^T[ACGT]AGG
             7892  CCTCAGG             
EcoO109I     4682  AGGACCT              [GA]G^G[ACGT]CC[CT]
             7426  AGGTCCC             
EcoRI         659  GAATTC               G^AATTC
             6497  GAATTC              
EcoT22I      2036  ATGCAT               ATGCA^T
             5178  ATGCAT              
FbaI           18  TGATCA               T^GATCA
             6456  TGATCA              
HaeII        7571  AGCGCC               [GA]GCGC^[CT]
             8495  AGCGCC              
HgaI          210  GCGTC                GACGC(5/10)
             5263  GCGTC               
HincII       5614  GTTGAC               GT[CT]^[GA]AC
             7320  GTCAAC              
HindII       5614  GTTGAC               GT[CT]^[GA]AC
             7320  GTCAAC              
MamI          302  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6902  GATCAGGATC          
NsiI         2036  ATGCAT               ATGCA^T
             5178  ATGCAT              
PaeR7I       6674  CTCGAG               C^TCGAG
             7676  CTCGAG              
PpuMI        4682  AGGACCT              [GA]G^G[AT]CC[CT]
             7426  AGGTCCC             
PshBI        3361  ATTAAT               AT^TAAT
             5182  ATTAAT              
RcaI          972  TCATGA               T^CATGA
             1278  TCATGA              
SspI         2221  AATATT               AAT^ATT
             3089  AATATT              
XhoI         6674  CTCGAG               C^TCGAG
             7676  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        EcoNI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XcmI        
XhoII       XmnI        
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