Restriction map for pF1KB0006
 (Circular) (Six-base) MAPSORT of: pF1KB0006.seq  Check: 4786  from: 1  to: 9908



pF1KB0006  9908 bp

 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1751    1751 
   Size:       9908
 
AccI GT'mk_AC

Cuts at:   1052    1781    2135    2518    3557    4130    4609    6946    1052 
   Size:        729     354     383    1039     573     479    2337    4014

  Fragments arranged by size: 

                4014    2337    1039     729     573     479     383     354
            
 
Acc65I G'GTAC_C

Cuts at:   6924    6924 
   Size:       9908
 
AclI AA'CG_TT

Cuts at:   9748    9748 
   Size:       9908
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     407    1096    2140    3488    4046    4281    5041    5785 
   Size:          6     689    1044    1348     558     235     760     744

Cuts at:   5785    7733    8165    8912    9180     401 
   Size:       1948     432     747     268    1129

  Fragments arranged by size: 

                1948    1348    1129    1044     760     747     744     689
                 558     432     268     235       6
 
AflII C'TTAA_G

Cuts at:   5221    5221 
   Size:       9908
 
AflIII A'CryG_T

Cuts at:    596    1597    6574    6625    7260    8364    9341     596 
   Size:       1001    4977      51     635    1104     977    1163

  Fragments arranged by size: 

                4977    1163    1104    1001     977     635      51
 
AgeI A'CCGG_T

Cuts at:   8296    8296 
   Size:       9908
 
AhdI GACnn_n'nnGTC

Cuts at:   1967    2346    1967 
   Size:        379    9529
 
AleI CACnn'nnGTG

Cuts at:    931    2221    2428    3397    3415    3673    4298     931 
   Size:       1290     207     969      18     258     625    6541

  Fragments arranged by size: 

                6541    1290     969     625     258     207      18
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    543    4259     543 
   Size:       3716    6192
 
AlwNI CAG_nnn'CTG

Cuts at:    156    1074    1804    2403    2539    2967    4450    4977    5467 
   Size:        918     730     599     136     428    1483     527     490

Cuts at:   5467    7213    8780     156 
   Size:       1746    1567    1284

  Fragments arranged by size: 

                1746    1567    1483    1284     918     730     599     527
                 490     428     136
 
ApaI G_GGCC'C

Cuts at:    128    3174    6117     128 
   Size:       3046    2943    3919

  Fragments arranged by size: 

                3919    3046    2943
 
ApaLI G'TGCA_C

Cuts at:   1593    4234    8678    1593 
   Size:       2641    4444    2823

  Fragments arranged by size: 

                4444    2823    2641
 
ApoI r'AATT_y

Cuts at:   1347    2052    3762    4338    6598    6912    9261    9517    1347 
   Size:        705    1710     576    2260     314    2349     256    1738

  Fragments arranged by size: 

                2349    2260    1738    1710     705     576     314     256
            
 
AseI AT'TA_AT

Cuts at:     20    4715      20 
   Size:       4695    5213
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9908
 
AvaI C'yCGr_G

Cuts at:    127    1337    3167    4250    4288    4310    6896    6928     127 
   Size:       1210    1830    1083      38      22    2586      32    3107

  Fragments arranged by size: 

                3107    2586    1830    1210    1083      38      32      22
            
 
AvrII C'CTAG_G

Cuts at:    315     315 
   Size:       9908
 
BamHI G'GATC_C

Cuts at:   4274    6933    4274 
   Size:       2659    7249
 
BanI G'GyrC_C

Cuts at:    168    1357    2806    3486    4323    6238    6494    6685    6924 
   Size:       1189    1449     680     837    1915     256     191     239

Cuts at:   6924    7338    7586    7621     168 
   Size:        414     248      35    2455

  Fragments arranged by size: 

                2455    1915    1449    1189     837     680     414     256
                 248     239     191      35
 
BanII G_rGCy'C

Cuts at:    128    3174    3334    3376    3799    3972    5298    5515    5789 
   Size:       3046     160      42     423     173    1326     217     274

Cuts at:   5789    6117    6922    7952     128 
   Size:        328     805    1030    2084

  Fragments arranged by size: 

                3046    2084    1326    1030     805     423     328     274
                 217     173     160      42
 
BbeI G_GCGC'C

Cuts at:   7342    7590    7342 
   Size:        248    9660
 
BbsI GAAGACnn'nnnn_

Cuts at:   1084    4170    5125    6649    1084 
   Size:       3086     955    1524    4343

  Fragments arranged by size: 

                4343    3086    1524     955
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     958     992    1640    1674    6274    6308      90 
   Size:         34     834      34     648      34    4600      34    3690

  Fragments arranged by size: 

                4600    3690     834     648      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3178    3309    7806    8567    9908    3178 
   Size:        131    4497     761    1341    3178

  Fragments arranged by size: 

                4497    3178    1341     761     131
 
BclI T'GATC_A

Cuts at:    669    3419    3789    4502    7428     669 
   Size:       2750     370     713    2926    3149

  Fragments arranged by size: 

                3149    2926    2750     713     370
 
BfrBI ATG'CAT

Cuts at:   1631    1631 
   Size:       9908
 
BglI GCCn_nnn'nGGC

Cuts at:   6722    6722 
   Size:       9908
 
BglII A'GATC_T

Cuts at:   5477    5864    7423    9460    5477 
   Size:        387    1559    2037    5925

  Fragments arranged by size: 

                5925    2037    1559     387
 
BlpI GC'TnA_GC

Cuts at:    206    7020     206 
   Size:       6814    3094
 
Bme1580I G_kGCm'C

Cuts at:    128     173    1597    1724    2831    3174    4238    4328    6117 
   Size:         45    1424     127    1107     343    1064      90    1789

Cuts at:   6117    7533    7626    8682     128 
   Size:       1416      93    1056    1354

  Fragments arranged by size: 

                1789    1424    1416    1354    1107    1064    1056     343
                 127      93      90      45
 
BmgBI CAC'GTC

Cuts at:   2868    2868 
   Size:       9908
 
BmrI ACTGGGnnnn_n'

Cuts at:    219     422    1814    5052    5963    6758    7120    7201    7536 
   Size:        203    1392    3238     911     795     362      81     335

Cuts at:   7536    9680     219 
   Size:       2144     447

  Fragments arranged by size: 

                3238    2144    1392     911     795     447     362     335
                 203      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    221    3086    3176    4169    4991    8275     221 
   Size:       2865      90     993     822    3284    1854

  Fragments arranged by size: 

                3284    2865    1854     993     822      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    588     859     864    2619    3100    3365    3524    3673    3692 
   Size:        271       5    1755     481     265     159     149      19

Cuts at:   3692    3850    4107    4349    4658    5290    5358     588 
   Size:        158     257     242     309     632      68    5138

  Fragments arranged by size: 

                5138    1755     632     481     309     271     265     257
                 242     159     158     149      68      19       5
 
Bpu10I CC'TnA_GC

Cuts at:    112    5050    5249    6450     112 
   Size:       4938     199    1201    3570

  Fragments arranged by size: 

                4938    3570    1201     199
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1914    7084    7963    8455    8753    1914 
   Size:       5170     879     492     298    3069

  Fragments arranged by size: 

                5170    3069     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    567    2037    2325    2334    3400    4299    5311    5666    9612 
   Size:       1470     288       9    1066     899    1012     355    3946

Cuts at:   9612     567 
   Size:        863

  Fragments arranged by size: 

                3946    1470    1066    1012     899     863     355     288
                   9
 
BsaAI yAC'GTr

Cuts at:   1109    1598    5617    6654    7261    1109 
   Size:        489    4019    1037     607    3756

  Fragments arranged by size: 

                4019    3756    1037     607     489
 
BsaBI GATnn'nnATC

Cuts at:      6    7422    9459       6 
   Size:       7416    2037     455

  Fragments arranged by size: 

                7416    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   3430    5531    7339    7587    8289    9785    3430 
   Size:       2101    1808     248     702    1496    3553

  Fragments arranged by size: 

                3553    2101    1808    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   5122    7097    7618    8296    8570    8717    9128    5122 
   Size:       1975     521     678     274     147     411    5902

  Fragments arranged by size: 

                5902    1975     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1947    1977    2083    2113    3420    3450    3954    3984    4821 
   Size:         30     106      30    1307      30     504      30     837

Cuts at:   4821    4851    1947 
   Size:         30    7004

  Fragments arranged by size: 

                7004    1307     837     504     106      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    585     917    1004    1355    2255    2395    3494    3711    3902 
   Size:        332      87     351     900     140    1099     217     191

Cuts at:   3902    4238    5654     585 
   Size:        336    1416    4839

  Fragments arranged by size: 

                4839    1416    1099     900     351     336     332     217
                 191     140      87
 
BseYI C'CCAG_C

Cuts at:    490     679    1007    3456    3900    4508    7333    8091    8668 
   Size:        189     328    2449     444     608    2825     758     577

Cuts at:   8668    9164     490 
   Size:        496    1234

  Fragments arranged by size: 

                2825    2449    1234     758     608     577     496     444
                 328     189
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    870     955     998    1212    1284    1293    1500    2924    3786 
   Size:         85      43     214      72       9     207    1424     862

Cuts at:   3786    4991    6555    6684     870 
   Size:       1205    1564     129    4094

  Fragments arranged by size: 

                4094    1564    1424    1205     862     214     207     129
                  85      72      43       9
 
BsiEI CG_ry'CG

Cuts at:    104     900    7450    7496    8704     104 
   Size:        796    6550      46    1208    1308

  Fragments arranged by size: 

                6550    1308    1208     796      46
 
BsiHKAI G_wGCw'C

Cuts at:   1597    2957    3376    3680    3703    3799    4150    4238    4295 
   Size:       1360     419     304      23      96     351      88      57

Cuts at:   4295    5515    6922    7700    7890    8682    1597 
   Size:       1220    1407     778     190     792    2823

  Fragments arranged by size: 

                2823    1407    1360    1220     792     778     419     351
                 304     190      96      88      57      23
 
BsmI GAATG_Cn'

Cuts at:   2004    2004 
   Size:       9908
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2242    5347    2242 
   Size:       3105    6803
 
Bsp1286I G_dGCh'C

Cuts at:    128     173    1597    1724    2831    2957    3174    3334    3376 
   Size:         45    1424     127    1107     126     217     160      42

Cuts at:   3376    3680    3703    3799    3972    4150    4238    4295    4328 
   Size:        304      23      96     173     178      88      57      33

Cuts at:   4328    5298    5515    5789    6117    6922    7533    7626    7700 
   Size:        970     217     274     328     805     611      93      74

Cuts at:   7700    7890    7952    8682     128 
   Size:        190      62     730    1354

  Fragments arranged by size: 

                1424    1354    1107     970     805     730     611     328
                 304     274     217     217     190     178     173     160
                 127     126      96      93      88      74      62      57
                  45      42      33      23
 
BspEI T'CCGG_A

Cuts at:   9128    9128 
   Size:       9908
 
BspHI T'CATG_A

Cuts at:   1710    6615    9084    1710 
   Size:       4905    2469    2534

  Fragments arranged by size: 

                4905    2534    2469
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    487     649     878    1751    2453    2852    2951    3932    4867 
   Size:        162     229     873     702     399      99     981     935

Cuts at:   4867    5570    6958    7172    7474    7855     487 
   Size:        703    1388     214     302     381    2540

  Fragments arranged by size: 

                2540    1388     981     935     873     703     702     399
                 381     302     229     214     162      99
 
BsrBI CCG'CTC

Cuts at:     50     306     950    8200    8254    9738    9903      50 
   Size:        256     644    7250      54    1484     165      55

  Fragments arranged by size: 

                7250    1484     644     256     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    327    1631    1899    5756    7820     327 
   Size:       1304     268    3857    2064    2415

  Fragments arranged by size: 

                3857    2415    2064    1304     268
 
BsrFI r'CCGG_y

Cuts at:   2911    5629    7906    8087    8296    2911 
   Size:       2718    2277     181     209    4523

  Fragments arranged by size: 

                4523    2718    2277     209     181
 
BsrGI T'GTAC_A

Cuts at:   1735    5486    6873    1735 
   Size:       3751    1387    4770

  Fragments arranged by size: 

                4770    3751    1387
 
BssSI C'ACGA_G

Cuts at:   1504    3304    3491    5297    6357    8179    8537    1504 
   Size:       1800     187    1806    1060    1822     358    2875

  Fragments arranged by size: 

                2875    1822    1806    1800    1060     358     187
 
BstAPI GCAn_nnn'nTGC

Cuts at:    779    2967    7285     779 
   Size:       2188    4318    3402

  Fragments arranged by size: 

                4318    3402    2188
 
BstBI TT'CG_AA

Cuts at:   1276    8269    1276 
   Size:       6993    2915
 
BstEII G'GTnAC_C

Cuts at:   2874    3018    5103    5829    2874 
   Size:        144    2085     726    6953

  Fragments arranged by size: 

                6953    2085     726     144
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1480    4002    5676    6505    1480 
   Size:       2522    1674     829    4883

  Fragments arranged by size: 

                4883    2522    1674     829
 
BstYI r'GATC_y

Cuts at:      1     952    2406    4274    5477    5864    6933    7400    7423 
   Size:        951    1454    1868    1203     387    1069     467      23

Cuts at:   7423    7758    8004    9016    9102    9114    9460       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1868    1454    1203    1069    1012     951     467     449
                 387     346     335     246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   2136    2136 
   Size:       9908
 
BtgI C'CryG_G

Cuts at:    176    1493    1881    3051    3197    4677    5165    6311     176 
   Size:       1317     388    1170     146    1480     488    1146    3773

  Fragments arranged by size: 

                3773    1480    1317    1170    1146     488     388     146
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    501    7858    8039    8202     501 
   Size:       7357     181     163    2207

  Fragments arranged by size: 

                7357    2207     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1075    2912    3050    3358    4079    5537    7151    1075 
   Size:       1837     138     308     721    1458    1614    3832

  Fragments arranged by size: 

                3832    1837    1614    1458     721     308     138
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   5526    5561    5526 
   Size:         35    9873
 
DraI TTT'AAA

Cuts at:   6432    6907    6432 
   Size:        475    9433
 
DrdI GACnn_nn'nnGTC

Cuts at:   2872    3627    7614    8472    2872 
   Size:        755    3987     858    4308

  Fragments arranged by size: 

                4308    3987     858     755
 
EaeI y'GGCC_r

Cuts at:    143     897    1023    1878    2169    2273    2454    3308    3750 
   Size:        754     126     855     291     104     181     854     442

Cuts at:   3750    4431    4674    5189    5394    5573    6032    6555    6783 
   Size:        681     243     515     205     179     459     523     228

Cuts at:   6783    7493    7667    8058    8085    9430     143 
   Size:        710     174     391      27    1345     621

  Fragments arranged by size: 

                1345     855     854     754     710     681     621     523
                 515     459     442     391     291     243     228     205
                 181     179     174     126     104      27
 
EagI C'GGCC_G

Cuts at:    897    7493     897 
   Size:       6596    3312
 
EarI CTCTTCn'nnn_

Cuts at:    184     383    1419    1709    3207    5773    6293    7931    8141 
   Size:        199    1036     290    1498    2566     520    1638     210

Cuts at:   8141     184 
   Size:       1951

  Fragments arranged by size: 

                2566    1951    1638    1498    1036     520     290     210
                 199
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4267    6027    8116    8426    8572    9718    4267 
   Size:       1760    2089     310     146    1146    4457

  Fragments arranged by size: 

                4457    2089    1760    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3374    3797    5513    6920    3374 
   Size:        423    1716    1407    6362

  Fragments arranged by size: 

                6362    1716    1407     423
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    401     407     588     859     864    1096    2140    2619    3100 
   Size:          6     181     271       5     232    1044     479     481

Cuts at:   3100    3365    3488    3524    3673    3692    3850    4046    4107 
   Size:        265     123      36     149      19     158     196      61

Cuts at:   4107    4281    4349    4658    5041    5290    5358    5785    7733 
   Size:        174      68     309     383     249      68     427    1948

Cuts at:   7733    8165    8912    9180     401 
   Size:        432     747     268    1129

  Fragments arranged by size: 

                1948    1129    1044     747     481     479     432     427
                 383     309     271     268     265     249     232     196
                 181     174     158     149     123      68      68      61
                  36      19       6       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2835    3693    4157    2835 
   Size:        858     464    8586

  Fragments arranged by size: 

                8586     858     464
 
EcoO109I rG'GnC_Cy

Cuts at:    124    2841    3241    4464    6114    7047     124 
   Size:       2717     400    1223    1650     933    2985

  Fragments arranged by size: 

                2985    2717    1650    1223     933     400
 
EcoRI G'AATT_C

Cuts at:   4338    6598    6912    4338 
   Size:       2260     314    7334

  Fragments arranged by size: 

                7334    2260     314
 
EcoRV GAT'ATC

Cuts at:   2486    5092    2486 
   Size:       2606    7302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   7927    7927 
   Size:       9908
 
FspI TGC'GCA

Cuts at:   7689    7689 
   Size:       9908
 
HaeII r_GCGC'y

Cuts at:   2539    5057    6732    7342    7590    8612    9591    2539 
   Size:       2518    1675     610     248    1022     979    2856

  Fragments arranged by size: 

                2856    2518    1675    1022     979     610     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1800    1832    1953    1985    2332    2364    2566    2598    3150 
   Size:         32     121      32     347      32     202      32     552

Cuts at:   3150    3182    3420    3452    3781    3813    4233    4244    4265 
   Size:         32     238      32     329      32     420      11      21

Cuts at:   4265    4266    4276    4298    4726    4758    5146    5178    5539 
   Size:          1      10      22     428      32     388      32     361

Cuts at:   5539    5571    5663    5695    5849    5881    6801    6833    7750 
   Size:         32      92      32     154      32     920      32     917

Cuts at:   7750    7780    7782    7812    1800 
   Size:         30       2      30    3896

  Fragments arranged by size: 

                3896     920     917     552     428     420     388     361
                 347     329     238     202     154     121      92      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      30      30      22      21
                  11      10       2       1
 
HincII GTy'rAC

Cuts at:   1782    2940    6327    6947    1782 
   Size:       1158    3387     620    4743

  Fragments arranged by size: 

                4743    3387    1158     620
 
KasI G'GCGC_C

Cuts at:   7338    7586    7338 
   Size:        248    9660
 
KpnI G_GTAC'C

Cuts at:   6928    6928 
   Size:       9908
 
MfeI C'AATT_G

Cuts at:   2585    3602    6848    2585 
   Size:       1017    3246    5645

  Fragments arranged by size: 

                5645    3246    1017
 
MluI A'CGCG_T

Cuts at:   9341    9341 
   Size:       9908
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    678    2047    2363    5582    6375    8579    8763     678 
   Size:       1369     316    3219     793    2204     184    1823

  Fragments arranged by size: 

                3219    2204    1823    1369     793     316     184
 
MscI TGG'CCA

Cuts at:   1025    2275    3310    4433    5191    5396    5575    6557    6785 
   Size:       1250    1035    1123     758     205     179     982     228

Cuts at:   6785    7669    1025 
   Size:        884    3264

  Fragments arranged by size: 

                3264    1250    1123    1035     982     884     758     228
                 205     179
 
MslI CAynn'nnrTG

Cuts at:    931    2221    2428    2881    3397    3415    3523    3673    4298 
   Size:       1290     207     453     516      18     108     150     625

Cuts at:   4298    4657    6256    6489    6503    7897    8024     931 
   Size:        359    1599     233      14    1394     127    2815

  Fragments arranged by size: 

                2815    1599    1394    1290     625     516     453     359
                 233     207     150     127     108      18      14
 
MspA1I CmG'CkG

Cuts at:    166     767     982    1130    1144    3904    4508    4625    6720 
   Size:        601     215     148      14    2760     604     117    2095

Cuts at:   6720    7019    7333    7693    8706    8951     166 
   Size:        299     314     360    1013     245    1123

  Fragments arranged by size: 

                2760    2095    1123    1013     604     601     360     314
                 299     245     215     148     117      14
 
NaeI GCC'GGC

Cuts at:   8089    8089 
   Size:       9908
 
NarI GG'CG_CC

Cuts at:   7339    7587    7339 
   Size:        248    9660
 
NcoI C'CATG_G

Cuts at:   1493    3051    1493 
   Size:       1558    8350
 
NgoMIV G'CCGG_C

Cuts at:   8087    8087 
   Size:       9908
 
NsiI A_TGCA'T

Cuts at:   1633    1633 
   Size:       9908
 
NspI r_CATG'y

Cuts at:    600    1631    1728    1742    6578    6707    6961    7461    8368 
   Size:       1031      97      14    4836     129     254     500     907

Cuts at:   8368     600 
   Size:       2140

  Fragments arranged by size: 

                4836    2140    1031     907     500     254     129      97
                  14
 
PasI CC'CwG_GG

Cuts at:   2837    3013    5914    2837 
   Size:        176    2901    6831

  Fragments arranged by size: 

                6831    2901     176
 
PciI A'CATG_T

Cuts at:    596    6574    8364     596 
   Size:       5978    1790    2140

  Fragments arranged by size: 

                5978    2140    1790
 
PflMI CCAn_nnn'nTGG

Cuts at:    156     467    1994    2885    3445    5336    5392    6110     156 
   Size:        311    1527     891     560    1891      56     718    3954

  Fragments arranged by size: 

                3954    1891    1527     891     718     560     311      56
            
 
PfoI T'CCnGG_A

Cuts at:   3540    4580    6122    6195    3540 
   Size:       1040    1542      73    7253

  Fragments arranged by size: 

                7253    1542    1040      73
 
PmeI GTTT'AAAC

Cuts at:   6907    6907 
   Size:       9908
 
PmlI CAC'GTG

Cuts at:   1109    1598    5617    1109 
   Size:        489    4019    5400

  Fragments arranged by size: 

                5400    4019     489
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   9047    9079    9047 
   Size:         32    9876
 
PpuMI rG'GwC_Cy

Cuts at:   2841    3241    4464    2841 
   Size:        400    1223    8285

  Fragments arranged by size: 

                8285    1223     400
 
PshAI GACnn'nnGTC

Cuts at:   1577    1577 
   Size:       9908
 
PspOMI G'GGCC_C

Cuts at:    124    3170    6113     124 
   Size:       3046    2943    3919

  Fragments arranged by size: 

                3919    3046    2943
 
PspXI vC'TCGA_Gb

Cuts at:   1337    4250    1337 
   Size:       2913    6995
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   5727    5759    5727 
   Size:         32    9876
 
PstI C_TGCA'G

Cuts at:   2849    3278    3365    4878    4992    6955    7640    2849 
   Size:        429      87    1513     114    1963     685    5117

  Fragments arranged by size: 

                5117    1963    1513     685     429     114      87
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9908
 
PvuII CAG'CTG

Cuts at:    982    1130    1144    3904    4508    7333    7693     982 
   Size:        148      14    2760     604    2825     360    3197

  Fragments arranged by size: 

                3197    2825    2760     604     360     148      14
 
RsrII CG'GwC_CG

Cuts at:   8103    8103 
   Size:       9908
 
SacI G_AGCT'C

Cuts at:   3376    3799    5515    6922    3376 
   Size:        423    1716    1407    6362

  Fragments arranged by size: 

                6362    1716    1407     423
 
SalI G'TCGA_C

Cuts at:   1780    6945    1780 
   Size:       5165    4743
 
SanDI GG'GwC_CC

Cuts at:   3241    3241 
   Size:       9908
 
SapI GCTCTTCn'nnn_

Cuts at:    184    5773    7931    8141     184 
   Size:       5589    2158     210    1951

  Fragments arranged by size: 

                5589    2158    1951     210
 
SbfI CC_TGCA'GG

Cuts at:   6955    6955 
   Size:       9908
 
ScaI AGT'ACT

Cuts at:   9425    9425 
   Size:       9908
 
SexAI A'CCwGG_T

Cuts at:   2721    2721 
   Size:       9908
 
SfcI C'TryA_G

Cuts at:     33     825    2057    2845    3274    3361    4269    4610    4874 
   Size:        792    1232     788     429      87     908     341     264

Cuts at:   4874    4988    6951    7636    8629    8820      33 
   Size:        114    1963     685     993     191    1121

  Fragments arranged by size: 

                1963    1232    1121     993     908     792     788     685
                 429     341     264     191     114      87
 
SfoI GGC'GCC

Cuts at:   7340    7588    7340 
   Size:        248    9660
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9908
 
SmaI CCC'GGG

Cuts at:    129    6930     129 
   Size:       6801    3107
 
SmlI C'TyrA_G

Cuts at:   1337    1929    4250    4310    5221    7063    7978    8470    8732 
   Size:        592    2321      60     911    1842     915     492     262

Cuts at:   8732    1337 
   Size:       2513

  Fragments arranged by size: 

                2513    2321    1842     915     911     592     492     262
                  60
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9908
 
SphI G_CATG'C

Cuts at:   6961    7461    6961 
   Size:        500    9408
 
SrfI GCCC'GGGC

Cuts at:    129     129 
   Size:       9908
 
StuI AGG'CCT

Cuts at:   2117    6227    2117 
   Size:       4110    5798
 
StyI C'CwwG_G

Cuts at:    315     723    1493    3051    5111    7042    7396     315 
   Size:        408     770    1558    2060    1931     354    2827

  Fragments arranged by size: 

                2827    2060    1931    1558     770     408     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    421    1735    2308    3448    4774    5055    5085    5598    5923 
   Size:       1314     573    1140    1326     281      30     513     325

Cuts at:   5923    7491    7624    8083    8292     421 
   Size:       1568     133     459     209    2037

  Fragments arranged by size: 

                2037    1568    1326    1314    1140     573     513     459
                 325     281     209     133      30
 
TatI w'GTAC_w

Cuts at:    334     407     745    1735    2027    2773    3230    3290    3833 
   Size:         73     338     990     292     746     457      60     543

Cuts at:   3833    5486    6137    6873    9423     334 
   Size:       1653     651     736    2550     819

  Fragments arranged by size: 

                2550    1653     990     819     746     736     651     543
                 457     338     292      73      60
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1599    1631    1947    1979    2083    2115    2345    2377    3954 
   Size:         32     316      32     104      32     230      32    1577

Cuts at:   3954    3986    5081    5113    5171    5203    6343    6375    9425 
   Size:         32    1095      32      58      32    1140      32    3050

Cuts at:   9425    9457    1599 
   Size:         32    2050

  Fragments arranged by size: 

                3050    2050    1577    1140    1095     316     230     104
                  58      32      32      32      32      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   7705    7705 
   Size:       9908
 
XbaI T'CTAG_A

Cuts at:    787    6487    6939     787 
   Size:       5700     452    3756

  Fragments arranged by size: 

                5700    3756     452
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1159    2313    5592    6391    7403    1159 
   Size:       1154    3279     799    1012    3664

  Fragments arranged by size: 

                3664    3279    1154    1012     799
 
XhoI C'TCGA_G

Cuts at:   1337    4250    4310    1337 
   Size:       2913      60    6935

  Fragments arranged by size: 

                6935    2913      60
 
XmaI C'CCGG_G

Cuts at:    127    6928     127 
   Size:       6801    3107
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AloI     AscI     BaeI    BbvCI     BmtI    BsiWI
   BssHII   Bsu36I     ClaI   DraIII     FseI    FspAI  HindIII     HpaI
     NdeI     NheI     NotI     NruI     PacI     PsiI    SacII     SfiI
    SgrAI    SnaBI     SspI     SwaI     XmnI     ZraI
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