Restriction map for pF1KB0006
 (Linear) (Six-base) MAPSORT of: KIBB0006.seq  Check: 5159  from: 1  to: 6795



KIBB0006.orf

 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1643    6795 
   Size:       1643    5152
 
AccI GT'mk_AC

Cuts at:      0     944    1673    2027    2410    3449    4022    4501    6795 
   Size:        944     729     354     383    1039     573     479    2294

  Fragments arranged by size: 

                2294    1039     944     729     573     479     383     354
            
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     299     988    2032    3380    3938    4173    4933 
   Size:        293       6     689    1044    1348     558     235     760

Cuts at:   4933    5677    6795 
   Size:        744    1118

  Fragments arranged by size: 

                1348    1118    1044     760     744     689     558     293
                 235       6
 
AflII C'TTAA_G

Cuts at:      0    5113    6795 
   Size:       5113    1682
 
AflIII A'CryG_T

Cuts at:      0     488    1489    6466    6517    6795 
   Size:        488    1001    4977      51     278

  Fragments arranged by size: 

                4977    1001     488     278      51
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1859    2238    6795 
   Size:       1859     379    4557

  Fragments arranged by size: 

                4557    1859     379
 
AleI CACnn'nnGTG

Cuts at:      0     823    2113    2320    3289    3307    3565    4190    6795 
   Size:        823    1290     207     969      18     258     625    2605

  Fragments arranged by size: 

                2605    1290     969     823     625     258     207      18
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     435    4151    6795 
   Size:        435    3716    2644

  Fragments arranged by size: 

                3716    2644     435
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     966    1696    2295    2431    2859    4342    4869 
   Size:         48     918     730     599     136     428    1483     527

Cuts at:   4869    5359    6795 
   Size:        490    1436

  Fragments arranged by size: 

                1483    1436     918     730     599     527     490     428
                 136      48
 
ApaI G_GGCC'C

Cuts at:      0      20    3066    6009    6795 
   Size:         20    3046    2943     786

  Fragments arranged by size: 

                3046    2943     786      20
 
ApaLI G'TGCA_C

Cuts at:      0    1485    4126    6795 
   Size:       1485    2641    2669

  Fragments arranged by size: 

                2669    2641    1485
 
ApoI r'AATT_y

Cuts at:      0    1239    1944    3654    4230    6490    6795 
   Size:       1239     705    1710     576    2260     305

  Fragments arranged by size: 

                2260    1710    1239     705     576     305
 
AseI AT'TA_AT

Cuts at:      0    4607    6795 
   Size:       4607    2188
 
AvaI C'yCGr_G

Cuts at:      0      19    1229    3059    4142    4180    4202    6788    6795 
   Size:         19    1210    1830    1083      38      22    2586       7

  Fragments arranged by size: 

                2586    1830    1210    1083      38      22      19       7
            
 
AvrII C'CTAG_G

Cuts at:      0     207    6795 
   Size:        207    6588
 
BamHI G'GATC_C

Cuts at:      0    4166    6795 
   Size:       4166    2629
 
BanI G'GyrC_C

Cuts at:      0      60    1249    2698    3378    4215    6130    6386    6577 
   Size:         60    1189    1449     680     837    1915     256     191

Cuts at:   6577    6795 
   Size:        218

  Fragments arranged by size: 

                1915    1449    1189     837     680     256     218     191
                  60
 
BanII G_rGCy'C

Cuts at:      0      20    3066    3226    3268    3691    3864    5190    5407 
   Size:         20    3046     160      42     423     173    1326     217

Cuts at:   5407    5681    6009    6795 
   Size:        274     328     786

  Fragments arranged by size: 

                3046    1326     786     423     328     274     217     173
                 160      42      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     976    4062    5017    6541    6795 
   Size:        976    3086     955    1524     254

  Fragments arranged by size: 

                3086    1524     976     955     254
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     850     884    1532    1566    6166    6200    6795 
   Size:        850      34     648      34    4600      34     595

  Fragments arranged by size: 

                4600     850     648     595      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3070    3201    6795 
   Size:       3070     131    3594

  Fragments arranged by size: 

                3594    3070     131
 
BclI T'GATC_A

Cuts at:      0     561    3311    3681    4394    6795 
   Size:        561    2750     370     713    2401

  Fragments arranged by size: 

                2750    2401     713     561     370
 
BfrBI ATG'CAT

Cuts at:      0    1523    6795 
   Size:       1523    5272
 
BglI GCCn_nnn'nGGC

Cuts at:      0    6614    6795 
   Size:       6614     181
 
BglII A'GATC_T

Cuts at:      0    5369    5756    6795 
   Size:       5369     387    1039

  Fragments arranged by size: 

                5369    1039     387
 
BlpI GC'TnA_GC

Cuts at:      0      98    6795 
   Size:         98    6697
 
Bme1580I G_kGCm'C

Cuts at:      0      20      65    1489    1616    2723    3066    4130    4220 
   Size:         20      45    1424     127    1107     343    1064      90

Cuts at:   4220    6009    6795 
   Size:       1789     786

  Fragments arranged by size: 

                1789    1424    1107    1064     786     343     127      90
                  45      20
 
BmgBI CAC'GTC

Cuts at:      0    2760    6795 
   Size:       2760    4035
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     314    1706    4944    5855    6650    6795 
   Size:        111     203    1392    3238     911     795     145

  Fragments arranged by size: 

                3238    1392     911     795     203     145     111
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     113    2978    3068    4061    4883    6795 
   Size:        113    2865      90     993     822    1912

  Fragments arranged by size: 

                2865    1912     993     822     113      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     751     756    2511    2992    3257    3416    3565 
   Size:        480     271       5    1755     481     265     159     149

Cuts at:   3565    3584    3742    3999    4241    4550    5182    5250    6795 
   Size:         19     158     257     242     309     632      68    1545

  Fragments arranged by size: 

                1755    1545     632     481     480     309     271     265
                 257     242     159     158     149      68      19       5
            
 
Bpu10I CC'TnA_GC

Cuts at:      0       4    4942    5141    6342    6795 
   Size:          4    4938     199    1201     453

  Fragments arranged by size: 

                4938    1201     453     199       4
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1806    6795 
   Size:       1806    4989
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     459    1929    2217    2226    3292    4191    5203    5558 
   Size:        459    1470     288       9    1066     899    1012     355

Cuts at:   5558    6795 
   Size:       1237

  Fragments arranged by size: 

                1470    1237    1066    1012     899     459     355     288
                   9
 
BsaAI yAC'GTr

Cuts at:      0    1001    1490    5509    6546    6795 
   Size:       1001     489    4019    1037     249

  Fragments arranged by size: 

                4019    1037    1001     489     249
 
BsaHI Gr'CG_yC

Cuts at:      0    3322    5423    6795 
   Size:       3322    2101    1372

  Fragments arranged by size: 

                3322    2101    1372
 
BsaWI w'CCGG_w

Cuts at:      0    5014    6795 
   Size:       5014    1781
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1839    1869    1975    2005    3312    3342    3846    3876 
   Size:       1839      30     106      30    1307      30     504      30

Cuts at:   3876    4713    4743    6795 
   Size:        837      30    2052

  Fragments arranged by size: 

                2052    1839    1307     837     504     106      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     477     809     896    1247    2147    2287    3386    3603 
   Size:        477     332      87     351     900     140    1099     217

Cuts at:   3603    3794    4130    5546    6795 
   Size:        191     336    1416    1249

  Fragments arranged by size: 

                1416    1249    1099     900     477     351     336     332
                 217     191     140      87
 
BseYI C'CCAG_C

Cuts at:      0     382     571     899    3348    3792    4400    6795 
   Size:        382     189     328    2449     444     608    2395

  Fragments arranged by size: 

                2449    2395     608     444     382     328     189
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     847     890    1104    1176    1185    1392    2816 
   Size:        762      85      43     214      72       9     207    1424

Cuts at:   2816    3678    4883    6447    6576    6795 
   Size:        862    1205    1564     129     219

  Fragments arranged by size: 

                1564    1424    1205     862     762     219     214     207
                 129      85      72      43       9
 
BsiEI CG_ry'CG

Cuts at:      0     792    6795 
   Size:        792    6003
 
BsiHKAI G_wGCw'C

Cuts at:      0    1489    2849    3268    3572    3595    3691    4042    4130 
   Size:       1489    1360     419     304      23      96     351      88

Cuts at:   4130    4187    5407    6795 
   Size:         57    1220    1388

  Fragments arranged by size: 

                1489    1388    1360    1220     419     351     304      96
                  88      57      23
 
BsmI GAATG_Cn'

Cuts at:      0    1896    6795 
   Size:       1896    4899
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2134    5239    6795 
   Size:       2134    3105    1556

  Fragments arranged by size: 

                3105    2134    1556
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      65    1489    1616    2723    2849    3066    3226 
   Size:         20      45    1424     127    1107     126     217     160

Cuts at:   3226    3268    3572    3595    3691    3864    4042    4130    4187 
   Size:         42     304      23      96     173     178      88      57

Cuts at:   4187    4220    5190    5407    5681    6009    6795 
   Size:         33     970     217     274     328     786

  Fragments arranged by size: 

                1424    1107     970     786     328     304     274     217
                 217     178     173     160     127     126      96      88
                  57      45      42      33      23      20
 
BspHI T'CATG_A

Cuts at:      0    1602    6507    6795 
   Size:       1602    4905     288

  Fragments arranged by size: 

                4905    1602     288
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     379     541     770    1643    2345    2744    2843    3824 
   Size:        379     162     229     873     702     399      99     981

Cuts at:   3824    4759    5462    6795 
   Size:        935     703    1333

  Fragments arranged by size: 

                1333     981     935     873     703     702     399     379
                 229     162      99
 
BsrBI CCG'CTC

Cuts at:      0     198     842    6795 
   Size:        198     644    5953

  Fragments arranged by size: 

                5953     644     198
 
BsrDI GCAATG_nn'

Cuts at:      0     219    1523    1791    5648    6795 
   Size:        219    1304     268    3857    1147

  Fragments arranged by size: 

                3857    1304    1147     268     219
 
BsrFI r'CCGG_y

Cuts at:      0    2803    5521    6795 
   Size:       2803    2718    1274

  Fragments arranged by size: 

                2803    2718    1274
 
BsrGI T'GTAC_A

Cuts at:      0    1627    5378    6765    6795 
   Size:       1627    3751    1387      30

  Fragments arranged by size: 

                3751    1627    1387      30
 
BssSI C'ACGA_G

Cuts at:      0    1396    3196    3383    5189    6249    6795 
   Size:       1396    1800     187    1806    1060     546

  Fragments arranged by size: 

                1806    1800    1396    1060     546     187
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     671    2859    6795 
   Size:        671    2188    3936

  Fragments arranged by size: 

                3936    2188     671
 
BstBI TT'CG_AA

Cuts at:      0    1168    6795 
   Size:       1168    5627
 
BstEII G'GTnAC_C

Cuts at:      0    2766    2910    4995    5721    6795 
   Size:       2766     144    2085     726    1074

  Fragments arranged by size: 

                2766    2085    1074     726     144
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1372    3894    5568    6397    6795 
   Size:       1372    2522    1674     829     398

  Fragments arranged by size: 

                2522    1674    1372     829     398
 
BstYI r'GATC_y

Cuts at:      0     844    2298    4166    5369    5756    6795 
   Size:        844    1454    1868    1203     387    1039

  Fragments arranged by size: 

                1868    1454    1203    1039     844     387
 
BstZ17I GTA'TAC

Cuts at:      0    2028    6795 
   Size:       2028    4767
 
BtgI C'CryG_G

Cuts at:      0      68    1385    1773    2943    3089    4569    5057    6203 
   Size:         68    1317     388    1170     146    1480     488    1146

Cuts at:   6203    6795 
   Size:        592

  Fragments arranged by size: 

                1480    1317    1170    1146     592     488     388     146
                  68
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     393    6795 
   Size:        393    6402
 
BtsI GCAGTG_nn'

Cuts at:      0     967    2804    2942    3250    3971    5429    6795 
   Size:        967    1837     138     308     721    1458    1366

  Fragments arranged by size: 

                1837    1458    1366     967     721     308     138
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    5418    5453    6795 
   Size:       5418      35    1342

  Fragments arranged by size: 

                5418    1342      35
 
DraI TTT'AAA

Cuts at:      0    6324    6795 
   Size:       6324     471
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2764    3519    6795 
   Size:       2764     755    3276

  Fragments arranged by size: 

                3276    2764     755
 
EaeI y'GGCC_r

Cuts at:      0      35     789     915    1770    2061    2165    2346    3200 
   Size:         35     754     126     855     291     104     181     854

Cuts at:   3200    3642    4323    4566    5081    5286    5465    5924    6447 
   Size:        442     681     243     515     205     179     459     523

Cuts at:   6447    6675    6795 
   Size:        228     120

  Fragments arranged by size: 

                 855     854     754     681     523     515     459     442
                 291     243     228     205     181     179     126     120
                 104      35
 
EagI C'GGCC_G

Cuts at:      0     789    6795 
   Size:        789    6006
 
EarI CTCTTCn'nnn_

Cuts at:      0      76     275    1311    1601    3099    5665    6185    6795 
   Size:         76     199    1036     290    1498    2566     520     610

  Fragments arranged by size: 

                2566    1498    1036     610     520     290     199      76
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    4159    5919    6795 
   Size:       4159    1760     876

  Fragments arranged by size: 

                4159    1760     876
 
EcoICRI GAG'CTC

Cuts at:      0    3266    3689    5405    6795 
   Size:       3266     423    1716    1390

  Fragments arranged by size: 

                3266    1716    1390     423
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     299     480     751     756     988    2032    2511 
   Size:        293       6     181     271       5     232    1044     479

Cuts at:   2511    2992    3257    3380    3416    3565    3584    3742    3938 
   Size:        481     265     123      36     149      19     158     196

Cuts at:   3938    3999    4173    4241    4550    4933    5182    5250    5677 
   Size:         61     174      68     309     383     249      68     427

Cuts at:   5677    6795 
   Size:       1118

  Fragments arranged by size: 

                1118    1044     481     479     427     383     309     293
                 271     265     249     232     196     181     174     158
                 149     123      68      68      61      36      19       6
                   5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2727    3585    4049    6795 
   Size:       2727     858     464    2746

  Fragments arranged by size: 

                2746    2727     858     464
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16    2733    3133    4356    6006    6795 
   Size:         16    2717     400    1223    1650     789

  Fragments arranged by size: 

                2717    1650    1223     789     400      16
 
EcoRI G'AATT_C

Cuts at:      0    4230    6490    6795 
   Size:       4230    2260     305

  Fragments arranged by size: 

                4230    2260     305
 
EcoRV GAT'ATC

Cuts at:      0    2378    4984    6795 
   Size:       2378    2606    1811

  Fragments arranged by size: 

                2606    2378    1811
 
HaeII r_GCGC'y

Cuts at:      0    2431    4949    6624    6795 
   Size:       2431    2518    1675     171

  Fragments arranged by size: 

                2518    2431    1675     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1692    1724    1845    1877    2224    2256    2458    2490 
   Size:       1692      32     121      32     347      32     202      32

Cuts at:   2490    3042    3074    3312    3344    3673    3705    4125    4136 
   Size:        552      32     238      32     329      32     420      11

Cuts at:   4136    4157    4158    4168    4190    4618    4650    5038    5070 
   Size:         21       1      10      22     428      32     388      32

Cuts at:   5070    5431    5463    5555    5587    5741    5773    6693    6725 
   Size:        361      32      92      32     154      32     920      32

Cuts at:   6725    6795 
   Size:         70

  Fragments arranged by size: 

                1692     920     552     428     420     388     361     347
                 329     238     202     154     121      92      70      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      22      21      11      10
                   1
 
HincII GTy'rAC

Cuts at:      0    1674    2832    6219    6795 
   Size:       1674    1158    3387     576

  Fragments arranged by size: 

                3387    1674    1158     576
 
MfeI C'AATT_G

Cuts at:      0    2477    3494    6740    6795 
   Size:       2477    1017    3246      55

  Fragments arranged by size: 

                3246    2477    1017      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1939    2255    5474    6267    6795 
   Size:        570    1369     316    3219     793     528

  Fragments arranged by size: 

                3219    1369     793     570     528     316
 
MscI TGG'CCA

Cuts at:      0     917    2167    3202    4325    5083    5288    5467    6449 
   Size:        917    1250    1035    1123     758     205     179     982

Cuts at:   6449    6677    6795 
   Size:        228     118

  Fragments arranged by size: 

                1250    1123    1035     982     917     758     228     205
                 179     118
 
MslI CAynn'nnrTG

Cuts at:      0     823    2113    2320    2773    3289    3307    3415    3565 
   Size:        823    1290     207     453     516      18     108     150

Cuts at:   3565    4190    4549    6148    6381    6395    6795 
   Size:        625     359    1599     233      14     400

  Fragments arranged by size: 

                1599    1290     823     625     516     453     400     359
                 233     207     150     108      18      14
 
MspA1I CmG'CkG

Cuts at:      0      58     659     874    1022    1036    3796    4400    4517 
   Size:         58     601     215     148      14    2760     604     117

Cuts at:   4517    6612    6795 
   Size:       2095     183

  Fragments arranged by size: 

                2760    2095     604     601     215     183     148     117
                  58      14
 
NcoI C'CATG_G

Cuts at:      0    1385    2943    6795 
   Size:       1385    1558    3852

  Fragments arranged by size: 

                3852    1558    1385
 
NsiI A_TGCA'T

Cuts at:      0    1525    6795 
   Size:       1525    5270
 
NspI r_CATG'y

Cuts at:      0     492    1523    1620    1634    6470    6599    6795 
   Size:        492    1031      97      14    4836     129     196

  Fragments arranged by size: 

                4836    1031     492     196     129      97      14
 
PasI CC'CwG_GG

Cuts at:      0    2729    2905    5806    6795 
   Size:       2729     176    2901     989

  Fragments arranged by size: 

                2901    2729     989     176
 
PciI A'CATG_T

Cuts at:      0     488    6466    6795 
   Size:        488    5978     329

  Fragments arranged by size: 

                5978     488     329
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      48     359    1886    2777    3337    5228    5284    6002 
   Size:         48     311    1527     891     560    1891      56     718

Cuts at:   6002    6795 
   Size:        793

  Fragments arranged by size: 

                1891    1527     891     793     718     560     311      56
                  48
 
PfoI T'CCnGG_A

Cuts at:      0    3432    4472    6014    6087    6795 
   Size:       3432    1040    1542      73     708

  Fragments arranged by size: 

                3432    1542    1040     708      73
 
PmlI CAC'GTG

Cuts at:      0    1001    1490    5509    6795 
   Size:       1001     489    4019    1286

  Fragments arranged by size: 

                4019    1286    1001     489
 
PpuMI rG'GwC_Cy

Cuts at:      0    2733    3133    4356    6795 
   Size:       2733     400    1223    2439

  Fragments arranged by size: 

                2733    2439    1223     400
 
PshAI GACnn'nnGTC

Cuts at:      0    1469    6795 
   Size:       1469    5326
 
PspOMI G'GGCC_C

Cuts at:      0      16    3062    6005    6795 
   Size:         16    3046    2943     790

  Fragments arranged by size: 

                3046    2943     790      16
 
PspXI vC'TCGA_Gb

Cuts at:      0    1229    4142    6795 
   Size:       1229    2913    2653

  Fragments arranged by size: 

                2913    2653    1229
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5619    5651    6795 
   Size:       5619      32    1144

  Fragments arranged by size: 

                5619    1144      32
 
PstI C_TGCA'G

Cuts at:      0    2741    3170    3257    4770    4884    6795 
   Size:       2741     429      87    1513     114    1911

  Fragments arranged by size: 

                2741    1911    1513     429     114      87
 
PvuII CAG'CTG

Cuts at:      0     874    1022    1036    3796    4400    6795 
   Size:        874     148      14    2760     604    2395

  Fragments arranged by size: 

                2760    2395     874     604     148      14
 
SacI G_AGCT'C

Cuts at:      0    3268    3691    5407    6795 
   Size:       3268     423    1716    1388

  Fragments arranged by size: 

                3268    1716    1388     423
 
SalI G'TCGA_C

Cuts at:      0    1672    6795 
   Size:       1672    5123
 
SanDI GG'GwC_CC

Cuts at:      0    3133    6795 
   Size:       3133    3662
 
SapI GCTCTTCn'nnn_

Cuts at:      0      76    5665    6795 
   Size:         76    5589    1130

  Fragments arranged by size: 

                5589    1130      76
 
SexAI A'CCwGG_T

Cuts at:      0    2613    6795 
   Size:       2613    4182
 
SfcI C'TryA_G

Cuts at:      0     717    1949    2737    3166    3253    4161    4502    4766 
   Size:        717    1232     788     429      87     908     341     264

Cuts at:   4766    4880    6795 
   Size:        114    1915

  Fragments arranged by size: 

                1915    1232     908     788     717     429     341     264
                 114      87
 
SmaI CCC'GGG

Cuts at:      0      21    6795 
   Size:         21    6774
 
SmlI C'TyrA_G

Cuts at:      0    1229    1821    4142    4202    5113    6795 
   Size:       1229     592    2321      60     911    1682

  Fragments arranged by size: 

                2321    1682    1229     911     592      60
 
SrfI GCCC'GGGC

Cuts at:      0      21    6795 
   Size:         21    6774
 
StuI AGG'CCT

Cuts at:      0    2009    6119    6795 
   Size:       2009    4110     676

  Fragments arranged by size: 

                4110    2009     676
 
StyI C'CwwG_G

Cuts at:      0     207     615    1385    2943    5003    6795 
   Size:        207     408     770    1558    2060    1792

  Fragments arranged by size: 

                2060    1792    1558     770     408     207
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     313    1627    2200    3340    4666    4947    4977    5490 
   Size:        313    1314     573    1140    1326     281      30     513

Cuts at:   5490    5815    6795 
   Size:        325     980

  Fragments arranged by size: 

                1326    1314    1140     980     573     513     325     313
                 281      30
 
TatI w'GTAC_w

Cuts at:      0     226     299     637    1627    1919    2665    3122    3182 
   Size:        226      73     338     990     292     746     457      60

Cuts at:   3182    3725    5378    6029    6765    6795 
   Size:        543    1653     651     736      30

  Fragments arranged by size: 

                1653     990     746     736     651     543     457     338
                 292     226      73      60      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1491    1523    1839    1871    1975    2007    2237    2269 
   Size:       1491      32     316      32     104      32     230      32

Cuts at:   2269    3846    3878    4973    5005    5063    5095    6235    6267 
   Size:       1577      32    1095      32      58      32    1140      32

Cuts at:   6267    6795 
   Size:        528

  Fragments arranged by size: 

                1577    1491    1140    1095     528     316     230     104
                  58      32      32      32      32      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0     679    6379    6795 
   Size:        679    5700     416

  Fragments arranged by size: 

                5700     679     416
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1051    2205    5484    6283    6795 
   Size:       1051    1154    3279     799     512

  Fragments arranged by size: 

                3279    1154    1051     799     512
 
XhoI C'TCGA_G

Cuts at:      0    1229    4142    4202    6795 
   Size:       1229    2913      60    2593

  Fragments arranged by size: 

                2913    2593    1229      60
 
XmaI C'CCGG_G

Cuts at:      0      19    6795 
   Size:         19    6776
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    AvrII    BamHI
     BanI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI     SalI    SanDI     SapI    SexAI     SfcI
     SmaI     SmlI     SrfI     StuI     StyI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AloI     AscI    AsiSI
     BaeI     BbeI    BbvCI     BmtI    BsaBI    BsiWI    BspEI   BssHII
   Bsu36I     ClaI   DraIII     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PmeI     PpiI     PsiI     PvuI
    RsrII    SacII     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     SwaI  Tth111I     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com