Restriction map for pF1KA1973
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AvrII       BamHI       BbrPI       
BbvCI       BcgI        BfrI        BlnI        BsaI        
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BstAPI      Cfr10I      ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SpeI        SphI        Sse8387I    
SspBI       SwaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         2863  GTATAC               GT^[AC][GT]AC
AccIII        204  TCCGGA               T^CCGGA
AflIII       2994  ACGTGT               A^C[GA][CT]GT
AhdI         1098  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        157  GTGCAC               G^TGCAC
AocI         1112  CCTTAGG              CC^T[ACGT]AGG
ApaLI         157  GTGCAC               G^TGCAC
AspEI        1098  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          61  TGCGCA               TGC^GCA
BclI          659  TGATCA               T^GATCA
BglII        2315  AGATCT               A^GATCT
BlpI           27  GCTGAGC              GC^T[ACGT]AGC
BpmI         3307  CTGGAG               CTGGAG(16/14)
Bpu1102I       27  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1162  TACGTA               [CT]AC^GT[GA]
BsaWI         204  TCCGGA               [AT]^CCGG[AT]
BseAI         204  TCCGGA               T^CCGGA
BspEI         204  TCCGGA               T^CCGGA
BspHI        3060  TCATGA               T^CATGA
BsrDI        1122  CATTGC               GCAATG(2/0)
BssSI         676  CACGAG               CACGAG(-5/-1)
Bst1107I     2863  GTATAC               GTA^TAC
BstBI        1015  TTCGAA               TT^CGAA
BstEII       1551  GGTTACC              G^GT[ACGT]ACC
BstPI        1551  GGTTACC              G^GT[ACGT]ACC
BstZ17I      2863  GTATAC               GTA^TAC
Bsu36I       1112  CCTTAGG              CC^T[ACGT]AGG
CelII          27  GCTGAGC              GC^T[ACGT]AGC
DraIII        136  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1098  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1112  CCTTAGG              CC^T[ACGT]AGG
EcoNI        2362  CCTTCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1551  GGTTACC              G^GT[ACGT]ACC
EcoT22I      2437  ATGCAT               ATGCA^T
FbaI          659  TGATCA               T^GATCA
FspI           61  TGCGCA               TGC^GCA
MroI          204  TCCGGA               T^CCGGA
NsiI         2437  ATGCAT               ATGCA^T
NspV         1015  TTCGAA               TT^CGAA
PaeR7I       3244  CTCGAG               C^TCGAG
PstI         3277  CTGCAG               CTGCA^G
RcaI         3060  TCATGA               T^CATGA
SapI         2323  GAAGAGC              GCTCTTC(1/4)
SfuI         1015  TTCGAA               TT^CGAA
SmaI          337  CCCGGG               CCC^GGG
SnaBI        1162  TACGTA               TAC^GTA
SspI         1292  AATATT               AAT^ATT
XbaI         1629  TCTAGA               T^CTAGA
XhoI         3244  CTCGAG               C^TCGAG
XmaCI         337  CCCGGG               C^CCGGG
XmaI          337  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          729  AAATTC               [GA]^AATT[CT]
             1711  AAATTC              
AlwNI          33  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3271  CAGGAGCTG           
ApoI          729  AAATTC               [GA]^AATT[CT]
             1711  AAATTC              
AspI          628  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3020  GACCCCGTC           
BbeI          267  GGCGCC               GGCGC^C
              346  GGCGCC              
BciVI        2614  GGATAC               GTATCC(6/5)
             1001  GTATCC              
BsaBI         453  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              660  GATCAGCATC          
DrdI          252  GACTAGGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3333  GACTTGTGAGTC        
HgaI          376  GACGC                GACGC(5/10)
             2518  GACGC               
KasI          267  GGCGCC               G^GCGCC
              346  GGCGCC              
MaeII        1163  ACGT                 A^CGT
             2994  ACGT                
MamI          453  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              660  GATCAGCATC          
NarI          267  GGCGCC               GG^CGCC
              346  GGCGCC              
NcoI          514  CCATGG               C^CATGG
             1415  CCATGG              
PflFI         628  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3020  GACCCCGTC           
PflMI         555  CCATACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1077  CCAGAATGTGG         
SfoI          267  GGCGCC               GGC^GCC
              346  GGCGCC              
StuI          468  AGGCCT               AGG^CCT
             1329  AGGCCT              
Tth111I       628  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3020  GACCCCGTC           
Van91I        555  CCATACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1077  CCAGAATGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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