Restriction map for pF1KA1973
 (Circular) (Six-base) MAPSORT of: pF1KA1973.seq  Check: 4207  from: 1  to: 6458



pF1KA1973  6458 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AccI GT'mk_AC

Cuts at:   2972    3496    2972 
   Size:        524    5934
 
Acc65I G'GTAC_C

Cuts at:   3474    3474 
   Size:       6458
 
AclI AA'CG_TT

Cuts at:   6298    6298 
   Size:       6458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1376    2243    2534    2623    4283    4715    5462    5730    1376 
   Size:        867     291      89    1660     432     747     268    2104

  Fragments arranged by size: 

                2104    1660     867     747     432     291     268      89
            
 
AflIII A'CryG_T

Cuts at:   3102    3810    4914    5891    3102 
   Size:        708    1104     977    3669

  Fragments arranged by size: 

                3669    1104     977     708
 
AgeI A'CCGG_T

Cuts at:   4846    4846 
   Size:       6458
 
AhdI GACnn_n'nnGTC

Cuts at:   1211    1211 
   Size:       6458
 
AleI CACnn'nnGTG

Cuts at:   1081    1081 
   Size:       6458
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1870    2186    1870 
   Size:        316    6142
 
AlwNI CAG_nnn'CTG

Cuts at:    146    3384    3763    5330     146 
   Size:       3238     379    1567    1274

  Fragments arranged by size: 

                3238    1567    1274     379
 
ApaLI G'TGCA_C

Cuts at:    265    5228     265 
   Size:       4963    1495
 
ApoI r'AATT_y

Cuts at:    837    1819    3462    5811    6067     837 
   Size:        982    1643    2349     256    1228

  Fragments arranged by size: 

                2349    1643    1228     982     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6458
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6458
 
AvaI C'yCGr_G

Cuts at:    445    2084    3352    3478     445 
   Size:       1639    1268     126    3425

  Fragments arranged by size: 

                3425    1639    1268     126
 
BamHI G'GATC_C

Cuts at:   3483    3483 
   Size:       6458
 
BanI G'GyrC_C

Cuts at:    189     375     454    3474    3888    4136    4171     189 
   Size:        186      79    3020     414     248      35    2476

  Fragments arranged by size: 

                3020    2476     414     248     186      79      35
 
BanII G_rGCy'C

Cuts at:    336     436     734    1229    2909    3272    3353    3472    4502 
   Size:        100     298     495    1680     363      81     119    1030

Cuts at:   4502     336 
   Size:       2292

  Fragments arranged by size: 

                2292    1680    1030     495     363     298     119     100
                  81
 
BbeI G_GCGC'C

Cuts at:    379     458    3892    4140     379 
   Size:         79    3434     248    2697

  Fragments arranged by size: 

                3434    2697     248      79
 
BbsI GAAGACnn'nnnn_

Cuts at:    929    1677    1880    2169    2238     929 
   Size:        748     203     289      69    5149

  Fragments arranged by size: 

                5149     748     289     203      69
 
BciVI GTATCCnnnnn_n'

Cuts at:   1120    2716    4356    5117    6458    1120 
   Size:       1596    1640     761    1341    1120

  Fragments arranged by size: 

                1640    1596    1341    1120     761
 
BclI T'GATC_A

Cuts at:    767    3978     767 
   Size:       3211    3247
 
BfrBI ATG'CAT

Cuts at:   2547    2547 
   Size:       6458
 
BglI GCCn_nnn'nGGC

Cuts at:    571    1224    1724    1987     571 
   Size:        653     500     263    5042

  Fragments arranged by size: 

                5042     653     500     263
 
BglII A'GATC_T

Cuts at:   2423    3973    6010    2423 
   Size:       1550    2037    2871

  Fragments arranged by size: 

                2871    2037    1550
 
BlpI GC'TnA_GC

Cuts at:    136    3570     136 
   Size:       3434    3024
 
Bme1580I G_kGCm'C

Cuts at:    194     245     269    1720    1814    1943    4083    4176    5232 
   Size:         51      24    1451      94     129    2140      93    1056

Cuts at:   5232     194 
   Size:       1420

  Fragments arranged by size: 

                2140    1451    1420    1056     129      94      93      51
                  24
 
BmrI ACTGGGnnnn_n'

Cuts at:   1131    1958    3670    3751    4086    6230    1131 
   Size:        827    1712      81     335    2144    1359

  Fragments arranged by size: 

                2144    1712    1359     827     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4825    4825 
   Size:       6458
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3436    3436 
   Size:       6458
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    214    1050    1072    1671    3634    4513    5005    5303     214 
   Size:        836      22     599    1963     879     492     298    1369

  Fragments arranged by size: 

                1963    1369     879     836     599     492     298      22
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   6162    6162 
   Size:       6458
 
BsaAI yAC'GTr

Cuts at:   1272    3811    1272 
   Size:       2539    3919
 
BsaBI GATnn'nnATC

Cuts at:      6     565     772    3972    6009       6 
   Size:        559     207    3200    2037     455

  Fragments arranged by size: 

                3200    2037     559     455     207
 
BsaHI Gr'CG_yC

Cuts at:    376     455     485    2627    3889    4137    4839    6335     376 
   Size:         79      30    2142    1262     248     702    1496     499

  Fragments arranged by size: 

                2142    1496    1262     702     499     248      79      30
            
 
BsaWI w'CCGG_w

Cuts at:    312    3647    4168    4846    5120    5267    5678     312 
   Size:       3335     521     678     274     147     411    1092

  Fragments arranged by size: 

                3335    1092     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    105     135     457     487     625     655     997    1027    2082 
   Size:         30     322      30     138      30     342      30    1055

Cuts at:   2082    2112     105 
   Size:         30    4451

  Fragments arranged by size: 

                4451    1055     342     322     138      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    126     189     192     597     937    3433     126 
   Size:         63       3     405     340    2496    3151

  Fragments arranged by size: 

                3151    2496     405     340      63       3
 
BseYI C'CCAG_C

Cuts at:    193    1173    1348    2142    3219    3883    4641    5218    5714 
   Size:        980     175     794    1077     664     758     577     496

Cuts at:   5714     193 
   Size:        937

  Fragments arranged by size: 

                1077     980     937     794     758     664     577     496
                 175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    739    2000    2146     739 
   Size:       1261     146    5051

  Fragments arranged by size: 

                5051    1261     146
 
BsiEI CG_ry'CG

Cuts at:    104    4000    4046    5254     104 
   Size:       3896      46    1208    1308

  Fragments arranged by size: 

                3896    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    269     409     734    1268    2966    3272    3353    3472    4250 
   Size:        140     325     534    1698     306      81     119     778

Cuts at:   4250    4440    5232     269 
   Size:        190     792    1495

  Fragments arranged by size: 

                1698    1495     792     778     534     325     306     190
                 140     119      81
 
Bsp1286I G_dGCh'C

Cuts at:    194     245     269     336     409     436     734    1229    1268 
   Size:         51      24      67      73      27     298     495      39

Cuts at:   1268    1720    1814    1943    2909    2966    3272    3353    3472 
   Size:        452      94     129     966      57     306      81     119

Cuts at:   3472    4083    4176    4250    4440    4502    5232     194 
   Size:        611      93      74     190      62     730    1420

  Fragments arranged by size: 

                1420     966     730     611     495     452     306     298
                 190     129     119      94      93      81      74      73
                  67      62      57      51      39      27      24
 
BspEI T'CCGG_A

Cuts at:    312    5678     312 
   Size:       5366    1092
 
BspHI T'CATG_A

Cuts at:   3168    5634    3168 
   Size:       2466    3992
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3508    3722    4024    4405    3508 
   Size:        214     302     381    5561

  Fragments arranged by size: 

                5561     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4750    4804    6288    6453      50 
   Size:       4700      54    1484     165      55

  Fragments arranged by size: 

                4700    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1229    4370    1229 
   Size:       3141    3317
 
BsrFI r'CCGG_y

Cuts at:   4456    4637    4846    4456 
   Size:        181     209    6068

  Fragments arranged by size: 

                6068     209     181
 
BssSI C'ACGA_G

Cuts at:    784    4729    5087     784 
   Size:       3945     358    2155

  Fragments arranged by size: 

                3945    2155     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3835    3835 
   Size:       6458
 
BstBI TT'CG_AA

Cuts at:   1124    4819    1124 
   Size:       3695    2763
 
BstEII G'GTnAC_C

Cuts at:   1659    1659 
   Size:       6458
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2064    2129    2324    3105    2064 
   Size:         65     195     781    5417

  Fragments arranged by size: 

                5417     781     195      65
 
BstYI r'GATC_y

Cuts at:      1     224    1043    2423    3230    3483    3950    3973    4308 
   Size:        223     819    1380     807     253     467      23     335

Cuts at:   4308    4554    5566    5652    5664    6010       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1380    1012     819     807     467     449     346     335
                 253     246     223      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   2973    2973 
   Size:       6458
 
Bsu36I CC'TnA_GG

Cuts at:   1221    1221 
   Size:       6458
 
BtgI C'CryG_G

Cuts at:    478     500     622     668     793    1523     478 
   Size:         22     122      46     125     730    5413

  Fragments arranged by size: 

                5413     730     125     122      46      22
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4408    4589    4752    4408 
   Size:        181     163    6114

  Fragments arranged by size: 

                6114     181     163
 
BtsI GCAGTG_nn'

Cuts at:    636    2168    2514    3701     636 
   Size:       1532     346    1187    3393

  Fragments arranged by size: 

                3393    1532    1187     346
 
DraI TTT'AAA

Cuts at:   3457    3457 
   Size:       6458
 
DraIII CAC_nnn'GTG

Cuts at:    249     249 
   Size:       6458
 
DrdI GACnn_nn'nnGTC

Cuts at:    366    3447    4164    5022     366 
   Size:       3081     717     858    1802

  Fragments arranged by size: 

                3081    1802     858     717
 
EaeI y'GGCC_r

Cuts at:    182     299     417     475     563    1594    1725    2119    2303 
   Size:        117     118      58      88    1031     131     394     184

Cuts at:   2303    4043    4217    4608    4635    5980     182 
   Size:       1740     174     391      27    1345     660

  Fragments arranged by size: 

                1740    1345    1031     660     394     391     184     174
                 131     118     117      88      58      27
 
EagI C'GGCC_G

Cuts at:   4043    4043 
   Size:       6458
 
EarI CTCTTCn'nnn_

Cuts at:   1764    2194    2426    3258    4481    4691    1764 
   Size:        430     232     832    1223     210    3531

  Fragments arranged by size: 

                3531    1223     832     430     232     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    478    1131    4666    4976    5122    6268     478 
   Size:        653    3535     310     146    1146     668

  Fragments arranged by size: 

                3535    1146     668     653     310     146
 
EcoICRI GAG'CTC

Cuts at:    732    3270    3351    3470     732 
   Size:       2538      81     119    3720

  Fragments arranged by size: 

                3720    2538     119      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1376    2243    2534    2623    3436    4283    4715    5462    5730 
   Size:        867     291      89     813     847     432     747     268

Cuts at:   5730    1376 
   Size:       2104

  Fragments arranged by size: 

                2104     867     847     813     747     432     291     268
                  89
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2474    2474 
   Size:       6458
 
EcoO109I rG'GnC_Cy

Cuts at:    468    1023    2081    3127    3597     468 
   Size:        555    1058    1046     470    3329

  Fragments arranged by size: 

                3329    1058    1046     555     470
 
EcoRI G'AATT_C

Cuts at:   3462    3462 
   Size:       6458
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1665    3458    4477    1665 
   Size:       1793    1019    3646

  Fragments arranged by size: 

                3646    1793    1019
 
FspI TGC'GCA

Cuts at:    171    4239     171 
   Size:       4068    2390
 
HaeII r_GCGC'y

Cuts at:    379     458     694    3892    4140    5162    6141     379 
   Size:         79     236    3198     248    1022     979     696

  Fragments arranged by size: 

                3198    1022     979     696     248     236      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    625     657     792     824    1197    1229    1659    1691    4300 
   Size:         32     135      32     373      32     430      32    2609

Cuts at:   4300    4330    4332    4362     625 
   Size:         30       2      30    2721

  Fragments arranged by size: 

                2721    2609     430     373     135      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   3497    3497 
   Size:       6458
 
KasI G'GCGC_C

Cuts at:    375     454    3888    4136     375 
   Size:         79    3434     248    2697

  Fragments arranged by size: 

                3434    2697     248      79
 
KpnI G_GTAC'C

Cuts at:   3478    3478 
   Size:       6458
 
MluI A'CGCG_T

Cuts at:   5891    5891 
   Size:       6458
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1350    1653    2305    3418    5129    5313    1350 
   Size:        303     652    1113    1711     184    2495

  Fragments arranged by size: 

                2495    1711    1113     652     303     184
 
MscI TGG'CCA

Cuts at:    477     565    1596    1727    2121    2305    4219     477 
   Size:         88    1031     131     394     184    1914    2716

  Fragments arranged by size: 

                2716    1914    1031     394     184     131      88
 
MslI CAynn'nnrTG

Cuts at:    763    1081    1369    2074    4447    4574     763 
   Size:        318     288     705    2373     127    2647

  Fragments arranged by size: 

                2647    2373     705     318     288     127
 
MspA1I CmG'CkG

Cuts at:   1556    3219    3390    3569    3883    4243    5256    5501    1556 
   Size:       1663     171     179     314     360    1013     245    2513

  Fragments arranged by size: 

                2513    1663    1013     360     314     245     179     171
            
 
NaeI GCC'GGC

Cuts at:   4639    4639 
   Size:       6458
 
NarI GG'CG_CC

Cuts at:    376     455    3889    4137     376 
   Size:         79    3434     248    2697

  Fragments arranged by size: 

                3434    2697     248      79
 
NcoI C'CATG_G

Cuts at:    622    1523     622 
   Size:        901    5557
 
NgoMIV G'CCGG_C

Cuts at:   4637    4637 
   Size:       6458
 
NsiI A_TGCA'T

Cuts at:   2549    2549 
   Size:       6458
 
NspI r_CATG'y

Cuts at:   3511    4011    4918    3511 
   Size:        500     907    5051

  Fragments arranged by size: 

                5051     907     500
 
PciI A'CATG_T

Cuts at:   4914    4914 
   Size:       6458
 
PflMI CCAn_nnn'nTGG

Cuts at:    669    1191     669 
   Size:        522    5936
 
PmeI GTTT'AAAC

Cuts at:   3457    3457 
   Size:       6458
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1325    1357    5597    5629    1325 
   Size:         32    4240      32    2154

  Fragments arranged by size: 

                4240    2154      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1023    2081    3127    1023 
   Size:       1058    1046    4354

  Fragments arranged by size: 

                4354    1058    1046
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    877     909     877 
   Size:         32    6426
 
PstI C_TGCA'G

Cuts at:   3389    3505    4190    3389 
   Size:        116     685    5657

  Fragments arranged by size: 

                5657     685     116
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6458
 
PvuII CAG'CTG

Cuts at:   1556    3219    3390    3883    4243    1556 
   Size:       1663     171     493     360    3771

  Fragments arranged by size: 

                3771    1663     493     360     171
 
RsrII CG'GwC_CG

Cuts at:   4653    4653 
   Size:       6458
 
SacI G_AGCT'C

Cuts at:    734    3272    3353    3472     734 
   Size:       2538      81     119    3720

  Fragments arranged by size: 

                3720    2538     119      81
 
SalI G'TCGA_C

Cuts at:   3495    3495 
   Size:       6458
 
SanDI GG'GwC_CC

Cuts at:   3127    3127 
   Size:       6458
 
SapI GCTCTTCn'nnn_

Cuts at:   2426    4481    4691    2426 
   Size:       2055     210    4193

  Fragments arranged by size: 

                4193    2055     210
 
SbfI CC_TGCA'GG

Cuts at:   3505    3505 
   Size:       6458
 
ScaI AGT'ACT

Cuts at:   5975    5975 
   Size:       6458
 
SfcI C'TryA_G

Cuts at:     33    1039    1304    3385    3501    4186    5179    5370      33 
   Size:       1006     265    2081     116     685     993     191    1121

  Fragments arranged by size: 

                2081    1121    1006     993     685     265     191     116
            
 
SfoI GGC'GCC

Cuts at:    377     456    3890    4138     377 
   Size:         79    3434     248    2697

  Fragments arranged by size: 

                3434    2697     248      79
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6458
 
SmaI CCC'GGG

Cuts at:    447    3480     447 
   Size:       3033    3425
 
SmlI C'TyrA_G

Cuts at:    229    1029    1051    1650    3352    3613    4528    5020    5282 
   Size:        800      22     599    1702     261     915     492     262

Cuts at:   5282     229 
   Size:       1405

  Fragments arranged by size: 

                1702    1405     915     800     599     492     262     261
                  22
 
SnaBI TAC'GTA

Cuts at:   1272    1272 
   Size:       6458
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6458
 
SphI G_CATG'C

Cuts at:   3511    4011    3511 
   Size:        500    5958
 
SspI AAT'ATT

Cuts at:   1402    1402 
   Size:       6458
 
StuI AGG'CCT

Cuts at:    578    1439     578 
   Size:        861    5597
 
StyI C'CwwG_G

Cuts at:    622     978    1523    2222    2475    3256    3592    3946     622 
   Size:        356     545     699     253     781     336     354    3134

  Fragments arranged by size: 

                3134     781     699     545     356     354     336     253
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1033    1089    1158    1948    2757    4041    4174    4633    4842 
   Size:         56      69     790     809    1284     133     459     209

Cuts at:   4842    1033 
   Size:       2649

  Fragments arranged by size: 

                2649    1284     809     790     459     209     133      69
                  56
 
TatI w'GTAC_w

Cuts at:   5973    5973 
   Size:       6458
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    457     489    5975    6007     457 
   Size:         32    5486      32     908

  Fragments arranged by size: 

                5486     908      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    739    3131    4255     739 
   Size:       2392    1124    2942

  Fragments arranged by size: 

                2942    2392    1124
 
XbaI T'CTAG_A

Cuts at:   1737    3489    1737 
   Size:       1752    4706
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3953    3953 
   Size:       6458
 
XhoI C'TCGA_G

Cuts at:   3352    3352 
   Size:       6458
 
XmaI C'CCGG_G

Cuts at:    445    3478     445 
   Size:       3033    3425
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI    PflMI     PmeI     PpiI    PpuMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI
    BsmBI    BsrGI   BssHII     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI
     PasI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI    SacII
    SexAI     SfiI    SgrAI     SrfI     SwaI     XmnI     ZraI
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