Restriction map for pF1KA1531
##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AccIII      AclI        AflIII      AgeI        AhdI        
AseI        AsnI        Asp700I     AspEI       AviII       
AvrII       BamHI       BbrPI       BclI        BlnI        
BsaAI       BseAI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BstBI       
ClaI        CpoI        DraI        DrdI        Eam1105I    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        MfeI        MluI        
MroI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SbfI        
ScaI        SexAI       SfuI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        3286  GACGTC               GACGT^C
Acc65I        842  GGTACC               G^GTACC
AcsI         1414  AAATTT               [GA]^AATT[CT]
AflII         544  CTTAAG               C^TTAAG
AlwNI         679  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      2474  AGCGCT               AGC^GCT
ApoI         1414  AAATTT               [GA]^AATT[CT]
Asp718I       842  GGTACC               G^GTACC
BciVI        1146  GTATCC               GTATCC(6/5)
BfrI          544  CTTAAG               C^TTAAG
BglII         411  AGATCT               A^GATCT
BsaBI        1296  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI         299  GCAATG               GCAATG(2/0)
BssSI        2583  CTCGTG               CACGAG(-5/-1)
Bst1107I     1666  GTATAC               GTA^TAC
BstAPI       1764  GCACATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2415  GGTGACC              G^GT[ACGT]ACC
BstPI        2415  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1666  GTATAC               GTA^TAC
DraIII       1657  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2474  AGCGCT               AGC^GCT
EcoO65I      2415  GGTGACC              G^GT[ACGT]ACC
KpnI          842  GGTACC               GGTAC^C
MamI         1296  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          545  TTAA                 T^TAA
NcoI          992  CCATGG               C^CATGG
NheI         1363  GCTAGC               G^CTAGC
SacI         1936  GAGCTC               GAGCT^C
SalI         1429  GTCGAC               G^TCGAC
SapI         3345  GAAGAGC              GCTCTTC(1/4)
SgrAI        1201  CGCCGGTG             C[GA]^CCGG[CT]G
SphI          672  GCATGC               GCATG^C
TaqI         1430  TCGA                 T^CGA
Tru9I         545  TTAA                 T^TAA
Tsp509I      1415  AATT                 ^AATT
TthHB8I      1430  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI         1429  GTCGAC               GT^[AC][GT]AC
             1666  GTATAC              
Alw44I        981  GTGCAC               G^TGCAC
             2875  GTGCAC              
AocI          342  CCTGAGG              CC^T[ACGT]AGG
              965  CCTCAGG             
ApaLI         981  GTGCAC               G^TGCAC
             2875  GTGCAC              
AscI           97  GGCGCGCC             GG^CGCGCC
             2588  GGCGCGCC            
AspI          939  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3283  GACGACGTC           
BalI         1469  TGGCCA               TGG^CCA
             2438  TGGCCA              
BbsI         3330  GAAGAC               GAAGAC(2/6)
             2542  GTCTTC              
BbvCI        1578  CCTCAGC              CCTCAGC(-5/-2)
             2265  GCTGAGG             
BcgI         1065  CGAGCGTGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1535  GCAGCCGCATCG        
BfaI         1364  CTAG                 C^TAG
             2404  CTAG                
BlpI          159  GCTGAGC              GC^T[ACGT]AGC
             1611  GCTGAGC             
Bpu1102I      159  GCTGAGC              GC^T[ACGT]AGC
             1611  GCTGAGC             
BpuAI        3330  GAAGAC               GAAGAC(2/6)
             2542  GTCTTC              
BsaI          448  GAGACC               GGTCTC(1/5)
             1039  GAGACC              
BseRI        1881  CTCCTC               GAGGAG(10/8)
             2260  GAGGAG              
BsiEI        1218  CGGCCG               CG[GA][CT]^CG
             2804  CGGCCG              
Bsp1407I     1280  TGTACA               T^GTACA
             1346  TGTACA              
BsrGI        1280  TGTACA               T^GTACA
             1346  TGTACA              
BstXI         809  CCAGCTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1625  CCAGGGAGGTGG        
BstYI         411  AGATCT               [GA]^GATC[CT]
             2168  GGATCT              
Bsu36I        342  CCTGAGG              CC^T[ACGT]AGG
              965  CCTCAGG             
CelII         159  GCTGAGC              GC^T[ACGT]AGC
             1611  GCTGAGC             
EagI         1218  CGGCCG               C^GGCCG
             2804  CGGCCG              
EclXI        1218  CGGCCG               C^GGCCG
             2804  CGGCCG              
Eco52I       1218  CGGCCG               C^GGCCG
             2804  CGGCCG              
Eco81I        342  CCTGAGG              CC^T[ACGT]AGG
              965  CCTCAGG             
EcoNI         693  CCTCCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1619  CCTTTCCCAGG         
HincII       1429  GTCGAC               GT[CT]^[GA]AC
             2062  GTCAAC              
HindII       1429  GTCGAC               GT[CT]^[GA]AC
             2062  GTCAAC              
KspI         1083  CCGCGG               CCGC^GG
             2615  CCGCGG              
MaeI         1364  CTAG                 C^TAG
             2404  CTAG                
MflI          411  AGATCT               [GA]^GATC[CT]
             2168  GGATCT              
MluNI        1469  TGGCCA               TGG^CCA
             2438  TGGCCA              
MscI         1469  TGGCCA               TGG^CCA
             2438  TGGCCA              
NspI          672  GCATGC               [GA]CATG^[CT]
             1766  ACATGC              
PflFI         939  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3283  GACGACGTC           
SacII        1083  CCGCGG               CCGC^GG
             2615  CCGCGG              
SfiI         2439  GGCCATGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3148  GGCCGGGCAGGCC       
SspBI        1280  TGTACA               T^GTACA
             1346  TGTACA              
StuI         1529  AGGCCT               AGG^CCT
             2930  AGGCCT              
Tth111I       939  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3283  GACGACGTC           
XhoII         411  AGATCT               [GA]^GATC[CT]
             2168  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BsaWI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StyI        
TfiI        TseI        Tsp45I      TspRI       Van91I      
XcmI        XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com