Restriction map for pF1KA1291
 (Linear) (Six-base) MAPSORT of: KIAA1291.seq  Check: 9300  from: 1  to: 3612



KIAA1291.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AccI GT'mk_AC

Cuts at:      0    2174    3612 
   Size:       2174    1438
 
Acc65I G'GTAC_C

Cuts at:      0    3320    3612 
   Size:       3320     292
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     289     552     772     976    1119    3612 
   Size:        187     102     263     220     204     143    2493

  Fragments arranged by size: 

                2493     263     220     204     187     143     102
 
AfeI AGC'GCT

Cuts at:      0      82    3080    3612 
   Size:         82    2998     532

  Fragments arranged by size: 

                2998     532      82
 
AflIII A'CryG_T

Cuts at:      0     216    3612 
   Size:        216    3396
 
AhdI GACnn_n'nnGTC

Cuts at:      0     711    3612 
   Size:        711    2901
 
AleI CACnn'nnGTG

Cuts at:      0    2957    3223    3612 
   Size:       2957     266     389

  Fragments arranged by size: 

                2957     389     266
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210    1021    2036    2307    3612 
   Size:        210     811    1015     271    1305

  Fragments arranged by size: 

                1305    1015     811     271     210
 
ApaLI G'TGCA_C

Cuts at:      0    2153    3612 
   Size:       2153    1459
 
ApoI r'AATT_y

Cuts at:      0    1437    1574    3612 
   Size:       1437     137    2038

  Fragments arranged by size: 

                2038    1437     137
 
AseI AT'TA_AT

Cuts at:      0    3085    3612 
   Size:       3085     527
 
AvaI C'yCGr_G

Cuts at:      0    1316    1781    2897    3612 
   Size:       1316     465    1116     715

  Fragments arranged by size: 

                1316    1116     715     465
 
AvrII C'CTAG_G

Cuts at:      0    2550    3612 
   Size:       2550    1062
 
BamHI G'GATC_C

Cuts at:      0    1359    1632    2133    3612 
   Size:       1359     273     501    1479

  Fragments arranged by size: 

                1479    1359     501     273
 
BanI G'GyrC_C

Cuts at:      0    2019    3320    3612 
   Size:       2019    1301     292

  Fragments arranged by size: 

                2019    1301     292
 
BanII G_rGCy'C

Cuts at:      0    1131    3612 
   Size:       1131    2481
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     628     802     851    1476    1710    2475    3612 
   Size:        628     174      49     625     234     765    1137

  Fragments arranged by size: 

                1137     765     628     625     234     174      49
 
BbvCI CC'TCA_GC

Cuts at:      0    3015    3612 
   Size:       3015     597
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2745    3612 
   Size:       2745     867
 
BclI T'GATC_A

Cuts at:      0     368    3612 
   Size:        368    3244
 
BfrBI ATG'CAT

Cuts at:      0     888    3612 
   Size:        888    2724
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2383    3612 
   Size:       2383    1229
 
BlpI GC'TnA_GC

Cuts at:      0    2622    3612 
   Size:       2622     990
 
Bme1580I G_kGCm'C

Cuts at:      0    1941    2157    3612 
   Size:       1941     216    1455

  Fragments arranged by size: 

                1941    1455     216
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     717    3612 
   Size:        717    2895
 
BmtI G_CTAG'C

Cuts at:      0    2377    3612 
   Size:       2377    1235
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2798    3612 
   Size:       2798     814
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1481    2149    2890    3346    3577    3612 
   Size:        772     709     668     741     456     231      35

  Fragments arranged by size: 

                 772     741     709     668     456     231      35
 
Bpu10I CC'TnA_GC

Cuts at:      0    3015    3612 
   Size:       3015     597
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     174    2030    2843    3612 
   Size:         85      89    1856     813     769

  Fragments arranged by size: 

                1856     813     769      89      85
 
BsaAI yAC'GTr

Cuts at:      0    3209    3612 
   Size:       3209     403
 
BsaBI GATnn'nnATC

Cuts at:      0     463    3612 
   Size:        463    3149
 
BsaWI w'CCGG_w

Cuts at:      0     848    3612 
   Size:        848    2764
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      55      85     247     277     745     775     917     947 
   Size:         55      30     162      30     468      30     142      30

Cuts at:    947    2122    2152    3612 
   Size:       1175      30    1460

  Fragments arranged by size: 

                1460    1175     468     162     142      55      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     337     938    1302    1815    1888    2023    2887 
   Size:        127     210     601     364     513      73     135     864

Cuts at:   2887    3154    3612 
   Size:        267     458

  Fragments arranged by size: 

                 864     601     513     458     364     267     210     135
                 127      73
 
BseYI C'CCAG_C

Cuts at:      0      77     984    1008    1384    1847    2236    2609    2967 
   Size:         77     907      24     376     463     389     373     358

Cuts at:   2967    3612 
   Size:        645

  Fragments arranged by size: 

                 907     645     463     389     376     373     358      77
                  24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1034    1522    1655    2277    3612 
   Size:       1034     488     133     622    1335

  Fragments arranged by size: 

                1335    1034     622     488     133
 
BsiHKAI G_wGCw'C

Cuts at:      0    1323    2157    2702    2720    2964    3612 
   Size:       1323     834     545      18     244     648

  Fragments arranged by size: 

                1323     834     648     545     244      18
 
BsmI GAATG_Cn'

Cuts at:      0    2180    2510    3612 
   Size:       2180     330    1102

  Fragments arranged by size: 

                2180    1102     330
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2972    3553    3612 
   Size:       2972     581      59

  Fragments arranged by size: 

                2972     581      59
 
Bsp1286I G_dGCh'C

Cuts at:      0    1131    1323    1941    2157    2702    2720    2964    3612 
   Size:       1131     192     618     216     545      18     244     648

  Fragments arranged by size: 

                1131     648     618     545     244     216     192      18
            
 
BspHI T'CATG_A

Cuts at:      0      56    3612 
   Size:         56    3556
 
BsrDI GCAATG_nn'

Cuts at:      0     815    1003    3458    3612 
   Size:        815     188    2455     154

  Fragments arranged by size: 

                2455     815     188     154
 
BsrFI r'CCGG_y

Cuts at:      0      86    3612 
   Size:         86    3526
 
BsrGI T'GTAC_A

Cuts at:      0    3073    3612 
   Size:       3073     539
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     913    2061    3612 
   Size:        913    1148    1551

  Fragments arranged by size: 

                1551    1148     913
 
BstBI TT'CG_AA

Cuts at:      0    1554    3612 
   Size:       1554    2058
 
BstEII G'GTnAC_C

Cuts at:      0    3006    3612 
   Size:       3006     606
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     392    1384    2406    3324    3612 
   Size:        392     992    1022     918     288

  Fragments arranged by size: 

                1022     992     918     392     288
 
BstYI r'GATC_y

Cuts at:      0     206     563    1359    1632    2133    3612 
   Size:        206     357     796     273     501    1479

  Fragments arranged by size: 

                1479     796     501     357     273     206
 
BstZ17I GTA'TAC

Cuts at:      0    2175    3612 
   Size:       2175    1437
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      19    1298    3612 
   Size:         19    1279    2314

  Fragments arranged by size: 

                2314    1279      19
 
BtsI GCAGTG_nn'

Cuts at:      0     728    1510    1793    3187    3307    3612 
   Size:        728     782     283    1394     120     305

  Fragments arranged by size: 

                1394     782     728     305     283     120
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3585    3612 
   Size:       3585      27
 
DraI TTT'AAA

Cuts at:      0     120    3401    3612 
   Size:        120    3281     211

  Fragments arranged by size: 

                3281     211     120
 
DraIII CAC_nnn'GTG

Cuts at:      0    2061    3612 
   Size:       2061    1551
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2479    3612 
   Size:       2479    1133
 
EaeI y'GGCC_r

Cuts at:      0     382    2027    2996    3593    3612 
   Size:        382    1645     969     597      19

  Fragments arranged by size: 

                1645     969     597     382      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     314     952    1110    1138    2104    2219    2707    3612 
   Size:        314     638     158      28     966     115     488     905

  Fragments arranged by size: 

                 966     905     638     488     314     158     115      28
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     891    3612 
   Size:        891    2721
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     289     552     772     976    1119    1481    2149 
   Size:        187     102     263     220     204     143     362     668

Cuts at:   2149    2890    3346    3577    3612 
   Size:        741     456     231      35

  Fragments arranged by size: 

                 741     668     456     362     263     231     220     204
                 187     143     102      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1711    2224    2554    3132    3612 
   Size:       1711     513     330     578     480

  Fragments arranged by size: 

                1711     578     513     480     330
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1770    2563    2598    3612 
   Size:       1770     793      35    1014

  Fragments arranged by size: 

                1770    1014     793      35
 
EcoRI G'AATT_C

Cuts at:      0    1437    3612 
   Size:       1437    2175
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1242    3612 
   Size:       1242    2370
 
HaeII r_GCGC'y

Cuts at:      0      84    3082    3435    3612 
   Size:         84    2998     353     177

  Fragments arranged by size: 

                2998     353     177      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     331     363     697     729     951     983 
   Size:         55      32     244      32     334      32     222      32

Cuts at:    983    3109    3141    3612 
   Size:       2126      32     471

  Fragments arranged by size: 

                2126     471     334     244     222      55      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2634    2894    3612 
   Size:       2634     260     718

  Fragments arranged by size: 

                2634     718     260
 
HindIII A'AGCT_T

Cuts at:      0    3394    3612 
   Size:       3394     218
 
HpaI GTT'AAC

Cuts at:      0    2894    3612 
   Size:       2894     718
 
KpnI G_GTAC'C

Cuts at:      0    3324    3612 
   Size:       3324     288
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     821    1434    3612 
   Size:        821     613    2178

  Fragments arranged by size: 

                2178     821     613
 
MscI TGG'CCA

Cuts at:      0     384    2029    2998    3595    3612 
   Size:        384    1645     969     597      17

  Fragments arranged by size: 

                1645     969     597     384      17
 
MslI CAynn'nnrTG

Cuts at:      0    1546    1828    2957    3065    3223    3612 
   Size:       1546     282    1129     108     158     389

  Fragments arranged by size: 

                1546    1129     389     282     158     108
 
MspA1I CmG'CkG

Cuts at:      0      36     802     996    3612 
   Size:         36     766     194    2616

  Fragments arranged by size: 

                2616     766     194      36
 
NdeI CA'TA_TG

Cuts at:      0    2771    3612 
   Size:       2771     841
 
NheI G'CTAG_C

Cuts at:      0    2373    3612 
   Size:       2373    1239
 
NsiI A_TGCA'T

Cuts at:      0     890    3612 
   Size:        890    2722
 
NspI r_CATG'y

Cuts at:      0     246     405    1352    1810    1875    2055    3612 
   Size:        246     159     947     458      65     180    1557

  Fragments arranged by size: 

                1557     947     458     246     180     159      65
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     555    2036    2974    3612 
   Size:        555    1481     938     638

  Fragments arranged by size: 

                1481     938     638     555
 
PfoI T'CCnGG_A

Cuts at:      0     209     749    3612 
   Size:        209     540    2863

  Fragments arranged by size: 

                2863     540     209
 
PpuMI rG'GwC_Cy

Cuts at:      0    2598    3612 
   Size:       2598    1014
 
PsiI TTA'TAA

Cuts at:      0    1880    2338    3612 
   Size:       1880     458    1274

  Fragments arranged by size: 

                1880    1274     458
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2334    2366    3612 
   Size:       2334      32    1246

  Fragments arranged by size: 

                2334    1246      32
 
PstI C_TGCA'G

Cuts at:      0    1452    2841    3416    3612 
   Size:       1452    1389     575     196

  Fragments arranged by size: 

                1452    1389     575     196
 
PvuII CAG'CTG

Cuts at:      0      36     996    3612 
   Size:         36     960    2616

  Fragments arranged by size: 

                2616     960      36
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1110    2104    2707    3612 
   Size:       1110     994     603     905

  Fragments arranged by size: 

                1110     994     905     603
 
SfcI C'TryA_G

Cuts at:      0    1448    1692    2837    3412    3612 
   Size:       1448     244    1145     575     200

  Fragments arranged by size: 

                1448    1145     575     244     200
 
SmaI CCC'GGG

Cuts at:      0    1783    3612 
   Size:       1783    1829
 
SmlI C'TyrA_G

Cuts at:      0     100     153    2045    2858    3612 
   Size:        100      53    1892     813     754

  Fragments arranged by size: 

                1892     813     754     100      53
 
SphI G_CATG'C

Cuts at:      0    1810    3612 
   Size:       1810    1802
 
SspI AAT'ATT

Cuts at:      0    1261    2646    3612 
   Size:       1261    1385     966

  Fragments arranged by size: 

                1385    1261     966
 
StuI AGG'CCT

Cuts at:      0    2126    3612 
   Size:       2126    1486
 
StyI C'CwwG_G

Cuts at:      0    1628    2399    2550    3482    3612 
   Size:       1628     771     151     932     130

  Fragments arranged by size: 

                1628     932     771     151     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      91     699    1000    1558    2176    3612 
   Size:         91     608     301     558     618    1436

  Fragments arranged by size: 

                1436     618     608     558     301      91
 
TatI w'GTAC_w

Cuts at:      0    3073    3612 
   Size:       3073     539
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     745     777    1068    1100    2122    2154    2234    2266 
   Size:        745      32     291      32    1022      32      80      32

Cuts at:   2266    3612 
   Size:       1346

  Fragments arranged by size: 

                1346    1022     745     291      80      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1209    3612 
   Size:       1209    2403
 
XmaI C'CCGG_G

Cuts at:      0    1781    3612 
   Size:       1781    1831
 
XmnI GAAnn'nnTTC

Cuts at:      0    1064    1574    1999    3612 
   Size:       1064     510     425    1613

  Fragments arranged by size: 

                1613    1064     510     425
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     AseI     AvaI    AvrII    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI
     MscI     MslI   MspA1I     NdeI     NheI     NsiI     NspI    PflMI
     PfoI    PpuMI     PsiI     PsrI     PstI    PvuII     SapI     SfcI
     SmaI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AlfI     AloI     ApaI
     AscI    AsiSI     BaeI     BbeI     BcgI    BglII    BmgBI     BsaI
    BsaHI    BsiEI    BsiWI    BspEI    BspMI    BsrBI   BssHII    BssSI
   Bsu36I     BtgI     ClaI     EagI  EcoICRI    EcoRV     FseI     FspI
    FspAI     KasI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI     XhoI     ZraI
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