Restriction map for pF1KA1111
 (Linear) (Six-base) MAPSORT of: KIAA1111.seq  Check: 7630  from: 1  to: 3180



KIAA1111.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2196    3180 
   Size:       2196     984
 
AccI GT'mk_AC

Cuts at:      0    1895    3180 
   Size:       1895    1285
 
Acc65I G'GTAC_C

Cuts at:      0     875    3180 
   Size:        875    2305
 
AclI AA'CG_TT

Cuts at:      0    1448    3180 
   Size:       1448    1732
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     453     511    1167    1517    1612    1726    2827 
   Size:        439      14      58     656     350      95     114    1101

Cuts at:   2827    3044    3180 
   Size:        217     136

  Fragments arranged by size: 

                1101     656     439     350     217     136     114      95
                  58      14
 
AflIII A'CryG_T

Cuts at:      0      33     179    3180 
   Size:         33     146    3001

  Fragments arranged by size: 

                3001     146      33
 
AleI CACnn'nnGTG

Cuts at:      0    1534    3180 
   Size:       1534    1646
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     532    3180 
   Size:        532    2648
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1704    2737    3180 
   Size:       1704    1033     443

  Fragments arranged by size: 

                1704    1033     443
 
ApaI G_GGCC'C

Cuts at:      0     284    3180 
   Size:        284    2896
 
ApoI r'AATT_y

Cuts at:      0     448     688    1936    2017    2616    3180 
   Size:        448     240    1248      81     599     564

  Fragments arranged by size: 

                1248     599     564     448     240      81
 
AvaI C'yCGr_G

Cuts at:      0    2699    3180 
   Size:       2699     481
 
BamHI G'GATC_C

Cuts at:      0    1058    3180 
   Size:       1058    2122
 
BanI G'GyrC_C

Cuts at:      0     117     862     875    1747    3053    3180 
   Size:        117     745      13     872    1306     127

  Fragments arranged by size: 

                1306     872     745     127     117      13
 
BanII G_rGCy'C

Cuts at:      0     284     361     380    1810    2380    3180 
   Size:        284      77      19    1430     570     800

  Fragments arranged by size: 

                1430     800     570     284      77      19
 
BbeI G_GCGC'C

Cuts at:      0    3057    3180 
   Size:       3057     123
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     355    1436    1462    1714    1937    3030    3180 
   Size:        355    1081      26     252     223    1093     150

  Fragments arranged by size: 

                1093    1081     355     252     223     150      26
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3154    3180 
   Size:       3154      26
 
BglII A'GATC_T

Cuts at:      0     899    1401    3180 
   Size:        899     502    1779

  Fragments arranged by size: 

                1779     899     502
 
BlpI GC'TnA_GC

Cuts at:      0    1165    3180 
   Size:       1165    2015
 
Bme1580I G_kGCm'C

Cuts at:      0     284     479    2398    3180 
   Size:        284     195    1919     782

  Fragments arranged by size: 

                1919     782     284     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2360    3180 
   Size:       2360     820
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     972    2364    2484    2584    3180 
   Size:         76     896    1392     120     100     596

  Fragments arranged by size: 

                1392     896     596     120     100      76
 
Bpu10I CC'TnA_GC

Cuts at:      0    2921    3180 
   Size:       2921     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     871    2176    2816    3180 
   Size:        871    1305     640     364

  Fragments arranged by size: 

                1305     871     640     364
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     783    1195    3180 
   Size:        783     412    1985

  Fragments arranged by size: 

                1985     783     412
 
BsaHI Gr'CG_yC

Cuts at:      0    1075    3054    3180 
   Size:       1075    1979     126

  Fragments arranged by size: 

                1979    1075     126
 
BsaWI w'CCGG_w

Cuts at:      0     380    3180 
   Size:        380    2800
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1261    1291    3180 
   Size:       1261      30    1889

  Fragments arranged by size: 

                1889    1261      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238    2116    2494    2497    2500    2503    2889    2892 
   Size:        238    1878     378       3       3       3     386       3

Cuts at:   2892    2906    3180 
   Size:         14     274

  Fragments arranged by size: 

                1878     386     378     274     238      14       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0    1491    2244    2254    2391    2420    2750    3047    3180 
   Size:       1491     753      10     137      29     330     297     133

  Fragments arranged by size: 

                1491     753     330     297     137     133      29      10
            
 
BsiEI CG_ry'CG

Cuts at:      0      81    3141    3180 
   Size:         81    3060      39

  Fragments arranged by size: 

                3060      81      39
 
BsiHKAI G_wGCw'C

Cuts at:      0     380    1171    2380    3180 
   Size:        380     791    1209     800

  Fragments arranged by size: 

                1209     800     791     380
 
BsmI GAATG_Cn'

Cuts at:      0    2177    3180 
   Size:       2177    1003
 
Bsp1286I G_dGCh'C

Cuts at:      0     284     361     380     479    1171    1810    2380    2398 
   Size:        284      77      19      99     692     639     570      18

Cuts at:   2398    3180 
   Size:        782

  Fragments arranged by size: 

                 782     692     639     570     284      99      77      19
                  18
 
BspEI T'CCGG_A

Cuts at:      0     380    3180 
   Size:        380    2800
 
BspHI T'CATG_A

Cuts at:      0     164     815    3180 
   Size:        164     651    2365

  Fragments arranged by size: 

                2365     651     164
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2196    2306    3180 
   Size:       2196     110     874

  Fragments arranged by size: 

                2196     874     110
 
BsrBI CCG'CTC

Cuts at:      0    2974    3180 
   Size:       2974     206
 
BsrFI r'CCGG_y

Cuts at:      0      76     120     137    1495    2319    3180 
   Size:         76      44      17    1358     824     861

  Fragments arranged by size: 

                1358     861     824      76      44      17
 
BsrGI T'GTAC_A

Cuts at:      0     798    3180 
   Size:        798    2382
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2285    2737    3180 
   Size:       2285     452     443

  Fragments arranged by size: 

                2285     452     443
 
BstBI TT'CG_AA

Cuts at:      0     738    3180 
   Size:        738    2442
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2462    3024    3163    3180 
   Size:       2462     562     139      17

  Fragments arranged by size: 

                2462     562     139      17
 
BstYI r'GATC_y

Cuts at:      0     307     899    1058    1364    1401    1415    1484    3180 
   Size:        307     592     159     306      37      14      69    1696

  Fragments arranged by size: 

                1696     592     307     306     159      69      37      14
            
 
BtgI C'CryG_G

Cuts at:      0     303     443    1290    2632    3180 
   Size:        303     140     847    1342     548

  Fragments arranged by size: 

                1342     847     548     303     140
 
BtsI GCAGTG_nn'

Cuts at:      0     252    1555    2376    2391    3180 
   Size:        252    1303     821      15     789

  Fragments arranged by size: 

                1303     821     789     252      15
 
DraIII CAC_nnn'GTG

Cuts at:      0    1055    3180 
   Size:       1055    2125
 
EaeI y'GGCC_r

Cuts at:      0      74      78     766    1406    1659    2291    2452    3116 
   Size:         74       4     688     640     253     632     161     664

Cuts at:   3116    3138    3180 
   Size:         22      42

  Fragments arranged by size: 

                 688     664     640     632     253     161      74      42
                  22       4
 
EagI C'GGCC_G

Cuts at:      0      78    3138    3180 
   Size:         78    3060      42

  Fragments arranged by size: 

                3060      78      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     649    1186    1612    1846    3180 
   Size:        215     434     537     426     234    1334

  Fragments arranged by size: 

                1334     537     434     426     234     215
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     904    1408    3180 
   Size:        904     504    1772

  Fragments arranged by size: 

                1772     904     504
 
EcoICRI GAG'CTC

Cuts at:      0     378    2378    3180 
   Size:        378    2000     802

  Fragments arranged by size: 

                2000     802     378
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     439     453     511     972    1167    1517    1612 
   Size:         76     363      14      58     461     195     350      95

Cuts at:   1612    1726    2364    2484    2584    2827    3044    3180 
   Size:        114     638     120     100     243     217     136

  Fragments arranged by size: 

                 638     461     363     350     243     217     195     136
                 120     114     100      95      76      58      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     493    1329    2236    3180 
   Size:        493     836     907     944

  Fragments arranged by size: 

                 944     907     836     493
 
EcoO109I rG'GnC_Cy

Cuts at:      0     281    1651    2215    2241    2696    2937    3111    3180 
   Size:        281    1370     564      26     455     241     174      69

  Fragments arranged by size: 

                1370     564     455     281     241     174      69      26
            
 
EcoRI G'AATT_C

Cuts at:      0     448     688    2616    3180 
   Size:        448     240    1928     564

  Fragments arranged by size: 

                1928     564     448     240
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1623    2167    3180 
   Size:       1623     544    1013

  Fragments arranged by size: 

                1623    1013     544
 
FseI GG_CCGG'CC

Cuts at:      0      80    3180 
   Size:         80    3100
 
HaeII r_GCGC'y

Cuts at:      0    2171    3057    3180 
   Size:       2171     886     123

  Fragments arranged by size: 

                2171     886     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1659    1691    1759    1791    2646    2678    2926    2958 
   Size:       1659      32      68      32     855      32     248      32

Cuts at:   2958    3180 
   Size:        222

  Fragments arranged by size: 

                1659     855     248     222      68      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     619    2149    3180 
   Size:        619    1530    1031

  Fragments arranged by size: 

                1530    1031     619
 
KasI G'GCGC_C

Cuts at:      0    3053    3180 
   Size:       3053     127
 
KpnI G_GTAC'C

Cuts at:      0     879    3180 
   Size:        879    2301
 
MluI A'CGCG_T

Cuts at:      0      33    3180 
   Size:         33    3147
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1464    3180 
   Size:       1464    1716
 
MscI TGG'CCA

Cuts at:      0     768    1408    1661    2293    3118    3180 
   Size:        768     640     253     632     825      62

  Fragments arranged by size: 

                 825     768     640     632     253      62
 
MslI CAynn'nnrTG

Cuts at:      0     820    1129    1358    1534    3022    3161    3180 
   Size:        820     309     229     176    1488     139      19

  Fragments arranged by size: 

                1488     820     309     229     176     139      19
 
MspA1I CmG'CkG

Cuts at:      0     650    2737    3051    3180 
   Size:        650    2087     314     129

  Fragments arranged by size: 

                2087     650     314     129
 
NaeI GCC'GGC

Cuts at:      0      78     139    1497    3180 
   Size:         78      61    1358    1683

  Fragments arranged by size: 

                1683    1358      78      61
 
NarI GG'CG_CC

Cuts at:      0    3054    3180 
   Size:       3054     126
 
NcoI C'CATG_G

Cuts at:      0    1290    2632    3180 
   Size:       1290    1342     548

  Fragments arranged by size: 

                1342    1290     548
 
NgoMIV G'CCGG_C

Cuts at:      0      76     137    1495    3180 
   Size:         76      61    1358    1685

  Fragments arranged by size: 

                1685    1358      76      61
 
NspI r_CATG'y

Cuts at:      0     183    2283    3180 
   Size:        183    2100     897

  Fragments arranged by size: 

                2100     897     183
 
PasI CC'CwG_GG

Cuts at:      0    2431    3180 
   Size:       2431     749
 
PciI A'CATG_T

Cuts at:      0     179    3180 
   Size:        179    3001
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1055    1211    3180 
   Size:       1055     156    1969

  Fragments arranged by size: 

                1969    1055     156
 
PfoI T'CCnGG_A

Cuts at:      0     452    1232    1367    3180 
   Size:        452     780     135    1813

  Fragments arranged by size: 

                1813     780     452     135
 
PpuMI rG'GwC_Cy

Cuts at:      0    2215    2696    3180 
   Size:       2215     481     484

  Fragments arranged by size: 

                2215     484     481
 
PspOMI G'GGCC_C

Cuts at:      0     280    3180 
   Size:        280    2900
 
PstI C_TGCA'G

Cuts at:      0    1481    1967    2303    2339    2733    3180 
   Size:       1481     486     336      36     394     447

  Fragments arranged by size: 

                1481     486     447     394     336      36
 
PvuII CAG'CTG

Cuts at:      0    2737    3180 
   Size:       2737     443
 
SacI G_AGCT'C

Cuts at:      0     380    2380    3180 
   Size:        380    2000     800

  Fragments arranged by size: 

                2000     800     380
 
SanDI GG'GwC_CC

Cuts at:      0    2696    3180 
   Size:       2696     484
 
SbfI CC_TGCA'GG

Cuts at:      0    2339    3180 
   Size:       2339     841
 
ScaI AGT'ACT

Cuts at:      0     808    3180 
   Size:        808    2372
 
SexAI A'CCwGG_T

Cuts at:      0     992    1283    3180 
   Size:        992     291    1897

  Fragments arranged by size: 

                1897     992     291
 
SfcI C'TryA_G

Cuts at:      0    1477    1963    2299    2335    2729    2761    3180 
   Size:       1477     486     336      36     394      32     419

  Fragments arranged by size: 

                1477     486     419     394     336      36      32
 
SfoI GGC'GCC

Cuts at:      0    3055    3180 
   Size:       3055     125
 
SmaI CCC'GGG

Cuts at:      0    2701    3180 
   Size:       2701     479
 
SmlI C'TyrA_G

Cuts at:      0     886    2191    2831    3180 
   Size:        886    1305     640     349

  Fragments arranged by size: 

                1305     886     640     349
 
SphI G_CATG'C

Cuts at:      0    2283    3180 
   Size:       2283     897
 
StuI AGG'CCT

Cuts at:      0    1589    2341    3180 
   Size:       1589     752     839

  Fragments arranged by size: 

                1589     839     752
 
StyI C'CwwG_G

Cuts at:      0    1290    1598    1646    2632    3180 
   Size:       1290     308      48     986     548

  Fragments arranged by size: 

                1290     986     548     308      48
 
TatI w'GTAC_w

Cuts at:      0     798     806     882    2966    3180 
   Size:        798       8      76    2084     214

  Fragments arranged by size: 

                2084     798     214      76       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1261    1293    2148    2180    2961    2993    3180 
   Size:       1261      32     855      32     781      32     187

  Fragments arranged by size: 

                1261     855     781     187      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1447    2205    2388    3111    3180 
   Size:       1447     758     183     723      69

  Fragments arranged by size: 

                1447     758     723     183      69
 
XmaI C'CCGG_G

Cuts at:      0    2699    3180 
   Size:       2699     481
 
XmnI GAAnn'nnTTC

Cuts at:      0     413    3180 
   Size:        413    2767
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI
    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BstAPI    BstBI    BstXI    BstYI     BtgI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FseI    HaeII    Hin4I  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfoI     SmaI     SmlI     SphI     StuI     StyI     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BmgBI     BmtI     BplI    BsaAI    BsaBI     BsgI    BsiWI
    BsmBI    BsrDI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI    EcoRV     FspI    FspAI   HincII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     SapI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI
     ZraI
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