Restriction map for pF1KA1097
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspHI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BciVI       BcnI        BfrI        BglI        BlnI        
BmyI        BsaAI       BsaHI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1286I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Tth111I     XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AseI         1021  ATTAAT               AT^TAAT
AsnI         1021  ATTAAT               AT^TAAT
Asp700I       727  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1402  CTCGAG               C^[CT]CG[GA]G
BanI         1257  GGCACC               G^G[CT][GA]CC
BcgI         1509  CGAAGCATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1977  TGATCA               T^GATCA
BglII        1416  AGATCT               A^GATCT
BlpI         1453  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1453  GCTAAGC              GC^T[ACGT]AGC
BsaBI        2503  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1405  GAGACC               GGTCTC(1/5)
BsaWI        2664  TCCGGT               [AT]^CCGG[AT]
BsgI         1366  GTGCAG               GTGCAG(16/14)
BsmFI        1579  GTCCC                GGGAC(10/14)
BsoBI        1402  CTCGAG               C^[CT]CG[GA]G
BspHI         756  TCATGA               T^CATGA
BsrDI         302  CATTGC               GCAATG(2/0)
CelII        1453  GCTAAGC              GC^T[ACGT]AGC
DraI          355  TTTAAA               TTT^AAA
EcoNI        1228  CCTAAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         727  GAATTC               G^AATTC
EcoRV        2082  GATATC               GAT^ATC
FbaI         1977  TGATCA               T^GATCA
HapII        2665  CCGG                 C^CGG
HindIII      2024  AAGCTT               A^AGCTT
HpaII        2665  CCGG                 C^CGG
MamI         2503  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       2312  CAGCTG               C[AC]G^C[GT]G
MspI         2665  CCGG                 C^CGG
NspI          124  GCATGT               [GA]CATG^[CT]
PaeR7I       1402  CTCGAG               C^TCGAG
PflMI        1429  CCAATTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2517  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1021  ATTAAT               AT^TAAT
PstI         2620  CTGCAG               CTGCA^G
PvuII        2312  CAGCTG               CAG^CTG
RcaI          756  TCATGA               T^CATGA
SalI         1081  GTCGAC               G^TCGAC
SapI         2046  GAAGAGC              GCTCTTC(1/4)
SexAI        2249  ACCTGGT              A^CC[AT]GGT
SspI          472  AATATT               AAT^ATT
Van91I       1429  CCAATTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          913  TCTAGA               T^CTAGA
XhoI         1402  CTCGAG               C^TCGAG
XmnI          727  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1081  GTCGAC               GT^[AC][GT]AC
             1167  GTCTAC              
AhdI         1384  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2309  GACCAGCTGTC         
AspEI        1384  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2309  GACCAGCTGTC         
BalI         1246  TGGCCA               TGG^CCA
             2589  TGGCCA              
BsaJI          78  CCTTGG               C^C[ACGT][ACGT]GG
              105  CCAAGG              
BsmI          495  GAATGC               GAATGC(1/-1)
             1461  GCATTC              
BspMI         582  ACCTGC               ACCTGC(4/8)
             2294  GCAGGT              
BstBI          11  TTCGAA               TT^CGAA
             1155  TTCGAA              
BstXI         112  CCAAATCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1522  CCACAAGGGTGG        
DraII        2517  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2640  AGGGCCT             
EaeI         1246  TGGCCA               [CT]^GGCC[GA]
             2589  TGGCCA              
Eam1105I     1384  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2309  GACCAGCTGTC         
EcoO109I     2517  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             2640  AGGGCCT             
EcoT14I        78  CCTTGG               C^C[AT][AT]GG
              105  CCAAGG              
HincII       1081  GTCGAC               GT[CT]^[GA]AC
             2317  GTCAAC              
HindII       1081  GTCGAC               GT[CT]^[GA]AC
             2317  GTCAAC              
MaeII        1209  ACGT                 A^CGT
             2030  ACGT                
MluNI        1246  TGGCCA               TGG^CCA
             2589  TGGCCA              
MscI         1246  TGGCCA               TGG^CCA
             2589  TGGCCA              
NspV           11  TTCGAA               TT^CGAA
             1155  TTCGAA              
PleI         1063  GACTC                GAGTC(4/5)
             2431  GACTC               
SfuI           11  TTCGAA               TT^CGAA
             1155  TTCGAA              
SmlI          722  CTCAAG               C^T[CT][GA]AG
             1402  CTCGAG              
StyI           78  CCTTGG               C^C[AT][AT]GG
              105  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbsI        BbvI        
BfaI        BpmI        BpuAI       BseRI       BsiYI       
BslI        BsmAI       Bsp1407I    BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspBI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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