Restriction map for pF1KA1097
 (Linear) (Six-base) MAPSORT of: KIAA1097.seq  Check: 4102  from: 1  to: 2733



KIAA1097.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0    1082    1168    2733 
   Size:       1082      86    1565

  Fragments arranged by size: 

                1565    1082      86
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     947    1058    1115    1816    2012    2104    2621    2666 
   Size:        947     111      57     701     196      92     517      45

Cuts at:   2666    2733 
   Size:         67

  Fragments arranged by size: 

                 947     701     517     196     111      92      67      57
                  45
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1389    2314    2733 
   Size:       1389     925     419

  Fragments arranged by size: 

                1389     925     419
 
AleI CACnn'nnGTG

Cuts at:      0    1527    2733 
   Size:       1527    1206
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2476    2733 
   Size:       2476     257
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2099    2131    2733 
   Size:       2099      32     602

  Fragments arranged by size: 

                2099     602      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     646    1374    2587    2733 
   Size:        646     728    1213     146

  Fragments arranged by size: 

                1213     728     646     146
 
ApoI r'AATT_y

Cuts at:      0     727    1032    1045    1969    2088    2152    2393    2613 
   Size:        727     305      13     924     119      64     241     220

Cuts at:   2613    2733 
   Size:        120

  Fragments arranged by size: 

                 924     727     305     241     220     120     119      64
                  13
 
AseI AT'TA_AT

Cuts at:      0    1022    2733 
   Size:       1022    1711
 
AvaI C'yCGr_G

Cuts at:      0    1402    2733 
   Size:       1402    1331
 
BanI G'GyrC_C

Cuts at:      0    1257    2733 
   Size:       1257    1476
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     839    1451    2252    2733 
   Size:        839     612     801     481

  Fragments arranged by size: 

                 839     801     612     481
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1498    1532    2733 
   Size:       1498      34    1201

  Fragments arranged by size: 

                1498    1201      34
 
BclI T'GATC_A

Cuts at:      0    1977    2733 
   Size:       1977     756
 
BglII A'GATC_T

Cuts at:      0    1416    2733 
   Size:       1416    1317
 
BlpI GC'TnA_GC

Cuts at:      0    1454    2733 
   Size:       1454    1279
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     359    2733 
   Size:        359    2374
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151    2420    2498    2733 
   Size:        151    2269      78     235

  Fragments arranged by size: 

                2269     235     151      78
 
Bpu10I CC'TnA_GC

Cuts at:      0    1847    2569    2733 
   Size:       1847     722     164

  Fragments arranged by size: 

                1847     722     164
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     707    2733 
   Size:        707    2026
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1399    2733 
   Size:       1399    1334
 
BsaBI GATnn'nnATC

Cuts at:      0    2507    2733 
   Size:       2507     226
 
BsaWI w'CCGG_w

Cuts at:      0    2664    2733 
   Size:       2664      69
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2099    2129    2733 
   Size:       2099      30     604

  Fragments arranged by size: 

                2099     604      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     377     832    2088    2733 
   Size:        377     455    1256     645

  Fragments arranged by size: 

                1256     645     455     377
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    2733 
   Size:       1387    1346
 
BsmI GAATG_Cn'

Cuts at:      0     501    1461    2733 
   Size:        501     960    1272

  Fragments arranged by size: 

                1272     960     501
 
BspHI T'CATG_A

Cuts at:      0     756    2733 
   Size:        756    1977
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     591    2285    2733 
   Size:        591    1694     448

  Fragments arranged by size: 

                1694     591     448
 
BsrDI GCAATG_nn'

Cuts at:      0     301    2733 
   Size:        301    2432
 
BsrGI T'GTAC_A

Cuts at:      0    1667    2010    2327    2733 
   Size:       1667     343     317     406

  Fragments arranged by size: 

                1667     406     343     317
 
BstBI TT'CG_AA

Cuts at:      0      12    1156    2733 
   Size:         12    1144    1577

  Fragments arranged by size: 

                1577    1144      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     119    1529    2733 
   Size:        119    1410    1204

  Fragments arranged by size: 

                1410    1204     119
 
BstYI r'GATC_y

Cuts at:      0     643     795    1178    1416    1827    2508    2733 
   Size:        643     152     383     238     411     681     225

  Fragments arranged by size: 

                 681     643     411     383     238     225     152
 
BtsI GCAGTG_nn'

Cuts at:      0    1375    2470    2733 
   Size:       1375    1095     263

  Fragments arranged by size: 

                1375    1095     263
 
DraI TTT'AAA

Cuts at:      0     357    2733 
   Size:        357    2376
 
EaeI y'GGCC_r

Cuts at:      0    1246    2589    2733 
   Size:       1246    1343     144

  Fragments arranged by size: 

                1343    1246     144
 
EarI CTCTTCn'nnn_

Cuts at:      0     239    1552    2041    2051    2733 
   Size:        239    1313     489      10     682

  Fragments arranged by size: 

                1313     682     489     239      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2371    2733 
   Size:       2371     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     947    1058    1115    1816    2012    2104    2420 
   Size:        151     796     111      57     701     196      92     316

Cuts at:   2420    2498    2621    2666    2733 
   Size:         78     123      45      67

  Fragments arranged by size: 

                 796     701     316     196     151     123     111      92
                  78      67      57      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1232    2733 
   Size:       1232    1501
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2518    2641    2733 
   Size:       2518     123      92

  Fragments arranged by size: 

                2518     123      92
 
EcoRI G'AATT_C

Cuts at:      0     727    2733 
   Size:        727    2006
 
EcoRV GAT'ATC

Cuts at:      0    2084    2733 
   Size:       2084     649
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     457     748    1639    2733 
   Size:        457     291     891    1094

  Fragments arranged by size: 

                1094     891     457     291
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     847     879    1048    1054    1080    1086    1375    1407 
   Size:        847      32     169       6      26       6     289      32

Cuts at:   1407    1863    1870    1895    1902    2250    2282    2300    2332 
   Size:        456       7      25       7     348      32      18      32

Cuts at:   2332    2733 
   Size:        401

  Fragments arranged by size: 

                 847     456     401     348     289     169      32      32
                  32      32      26      25      18       7       7       6
                   6
 
HincII GTy'rAC

Cuts at:      0    1083    2319    2733 
   Size:       1083    1236     414

  Fragments arranged by size: 

                1236    1083     414
 
HindIII A'AGCT_T

Cuts at:      0    2024    2733 
   Size:       2024     709
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     715     827    1501    2733 
   Size:        715     112     674    1232

  Fragments arranged by size: 

                1232     715     674     112
 
MscI TGG'CCA

Cuts at:      0    1248    2591    2733 
   Size:       1248    1343     142

  Fragments arranged by size: 

                1343    1248     142
 
MslI CAynn'nnrTG

Cuts at:      0    1157    1527    2213    2458    2733 
   Size:       1157     370     686     245     275

  Fragments arranged by size: 

                1157     686     370     275     245
 
MspA1I CmG'CkG

Cuts at:      0    2314    2733 
   Size:       2314     419
 
NdeI CA'TA_TG

Cuts at:      0     147    1515    1875    2733 
   Size:        147    1368     360     858

  Fragments arranged by size: 

                1368     858     360     147
 
NspI r_CATG'y

Cuts at:      0     128    2733 
   Size:        128    2605
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1435    2733 
   Size:       1435    1298
 
PfoI T'CCnGG_A

Cuts at:      0     347    2733 
   Size:        347    2386
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2099    2131    2733 
   Size:       2099      32     602

  Fragments arranged by size: 

                2099     602      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2518    2733 
   Size:       2518     215
 
PstI C_TGCA'G

Cuts at:      0    2624    2733 
   Size:       2624     109
 
PvuII CAG'CTG

Cuts at:      0    2314    2733 
   Size:       2314     419
 
SalI G'TCGA_C

Cuts at:      0    1081    2733 
   Size:       1081    1652
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2041    2733 
   Size:       2041     692
 
SexAI A'CCwGG_T

Cuts at:      0    2249    2733 
   Size:       2249     484
 
SfcI C'TryA_G

Cuts at:      0    1484    2040    2620    2733 
   Size:       1484     556     580     113

  Fragments arranged by size: 

                1484     580     556     113
 
SmlI C'TyrA_G

Cuts at:      0     722    1402    2733 
   Size:        722     680    1331

  Fragments arranged by size: 

                1331     722     680
 
SspI AAT'ATT

Cuts at:      0     474    2733 
   Size:        474    2259
 
StyI C'CwwG_G

Cuts at:      0      78     105    2733 
   Size:         78      27    2628

  Fragments arranged by size: 

                2628      78      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2714    2733 
   Size:       2714      19
 
TatI w'GTAC_w

Cuts at:      0    1667    1719    2010    2327    2733 
   Size:       1667      52     291     317     406

  Fragments arranged by size: 

                1667     406     317     291      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     635     667    1163    1195    2733 
   Size:        635      32     496      32    1538

  Fragments arranged by size: 

                1538     635     496      32      32
 
XbaI T'CTAG_A

Cuts at:      0     913    2733 
   Size:        913    1820
 
XhoI C'TCGA_G

Cuts at:      0    1402    2733 
   Size:       1402    1331
 
XmnI GAAnn'nnTTC

Cuts at:      0     731    2733 
   Size:        731    2002
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AlfI     AloI    AlwNI     ApoI
     AseI     AvaI     BanI     BbsI     BcgI     BclI    BglII     BlpI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI     BsgI     BsmI    BspHI    BspMI    BsrDI    BsrGI    BstBI
    BstXI    BstYI     BtsI     DraI     EaeI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NdeI     NspI    PflMI     PfoI
     PpiI    PpuMI     PstI    PvuII     SalI     SapI    SexAI     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI     XbaI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmtI
     BplI    BsaAI    BsaHI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I
     XcmI     XmaI     ZraI
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